Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,683,566	(C)_6→7	coding (1047/1404 nt)	fumC ←	fumarase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,683,560	1	.	C	91.6%	698.4 / 55.3	227	coding (1053/1404 nt)	fumC	fumarase C
Reads supporting (aligned to +/- strand): ref base . (10/9); new base C (104/104); total (114/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 255 total aligned reads displayed.

TTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCA  >  NC_000913/1683469‑1683651

  tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c                                                                                           >  2:8371227/1‑93 (MQ=255)

   ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑c                                                                                         >  1:2173403/1‑93 (MQ=255)

   ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑c                                                                                         <  1:977361/93‑1 (MQ=255)

    cATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGCACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑cc                                                                                        >  1:6328874/1‑93 (MQ=255)

      tGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑cccc                                                                                      <  2:2057362/93‑2 (MQ=255)

       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑cccca                                                                                     >  1:210275/1‑91 (MQ=255)

       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CC‑C‑CCCAt                                                                                     <  2:4662775/93‑1 (MQ=255)

        ccATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑ccccat                                                                                    >  2:3754929/1‑90 (MQ=255)

         cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑cc‑c‑ccccatg                                                                                   >  2:8594563/1‑89 (MQ=255)

           tCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGtt                                                                                  >  1:2521173/1‑93 (MQ=255)

           tCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGtt                                                                                  >  1:3040446/1‑93 (MQ=255)

              gCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGAt                                                                               <  2:6230800/93‑1 (MQ=255)

              gCCAGCAAGCGCACCGATTGCAGGAAATTGGTGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGAt                                                                               <  2:2112707/93‑1 (MQ=255)

               ccAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATc                                                                              >  2:5137565/1‑93 (MQ=255)

                cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCg                                                                             <  2:4836636/93‑1 (MQ=255)

                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGc                                                                            >  1:1223202/1‑93 (MQ=255)

                  gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGcc                                                                           >  2:1921935/1‑93 (MQ=255)

                  gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGcc                                                                           <  2:5932539/93‑1 (MQ=255)

                  gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGcc                                                                           <  2:6416193/93‑1 (MQ=255)

                   cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCa                                                                          <  1:3279186/93‑1 (MQ=255)

                   cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCa                                                                          >  1:7422785/1‑93 (MQ=255)

                   cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CC‑C‑CCCATGTTGATCGCCAc                                                                         <  1:402661/93‑1 (MQ=255)

                     aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcg                                                                        >  2:3312110/1‑93 (MQ=255)

                      gcgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgt                                                                       >  2:7607495/1‑93 (MQ=255)

                      gcgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgt                                                                       <  1:7661502/93‑1 (MQ=255)

                        gcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgtcg                                                                     <  1:8103218/93‑1 (MQ=255)

                         cACCGATTGCGGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgtcgt                                                                    <  1:4716725/93‑1 (MQ=255)

                         cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgtcgt                                                                    >  1:5354348/1‑93 (MQ=255)

                         cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgtcgt                                                                    >  1:4753069/1‑93 (MQ=255)

                         cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAcgtcgt                                                                    >  1:5528162/1‑93 (MQ=255)

                          aCCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGtt                                                                   >  2:3561777/1‑93 (MQ=255)

                            cGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTcc                                                                 >  2:5526874/1‑93 (MQ=255)

                             gATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTccc                                                                <  1:8578034/93‑1 (MQ=255)

                              aTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTcccc                                                               >  2:116419/1‑93 (MQ=255)

                              aTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTcccc                                                               <  2:5104829/93‑1 (MQ=255)

                                  cAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATc                                                            >  2:7926199/1‑93 (MQ=255)

                                    ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCAcc                                                         <  2:1691190/93‑1 (MQ=255)

                                    ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCAcc                                                         >  2:698799/1‑93 (MQ=255)

                                     gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                        <  2:23724/93‑1 (MQ=255)

                                     gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                        >  1:3136756/1‑93 (MQ=255)

                                       aaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                      >  2:5805797/1‑93 (MQ=255)

                                       aaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                      >  1:2545664/1‑93 (MQ=255)

                                         ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAca                                                    >  1:3688036/1‑93 (MQ=255)

                                          tgtgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                   >  1:8809204/1‑93 (MQ=255)

                                           gtgtATCCCCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                  <  2:1602515/93‑1 (MQ=255)

                                           gtgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                  >  2:4397517/1‑93 (MQ=255)

                                            tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagca                                                 >  1:5458915/1‑93 (MQ=255)

                                              gATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                               <  1:8708872/93‑1 (MQ=255)

                                              gATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                               <  2:4222145/93‑1 (MQ=255)

                                               gtCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag                                              <  2:2595907/92‑1 (MQ=255)

                                               aTCACCATTGGACGGAAGACGTTCAGTTCCAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTTATCGCCACGTCGTTCCCCATCACCTGCCCGCagag                                              >  1:5946945/1‑93 (MQ=255)

                                               aTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                             >  1:3914531/1‑93 (MQ=255)

                                                 cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                            >  2:7401026/1‑93 (MQ=255)

                                                  aCCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAt                                           >  1:4479715/1‑93 (MQ=255)

                                                  aCCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAt                                           >  2:676670/1‑93 (MQ=255)

                                                   ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                          >  2:3788866/1‑93 (MQ=255)

                                                   ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                          <  1:5726981/93‑1 (MQ=255)

                                                   ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                          <  1:51023/93‑1 (MQ=255)

                                                   ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                          <  1:3218708/93‑1 (MQ=255)

                                                    cATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                         >  1:206086/1‑93 (MQ=255)

                                                    cATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                         >  2:2854238/1‑93 (MQ=255)

                                                     aTTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                        >  1:1651201/1‑93 (MQ=255)

                                                     aTTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                        <  2:364569/93‑1 (MQ=255)

                                                     aTTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                        >  2:7748311/1‑93 (MQ=255)

                                                      ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGtt                                       <  2:5595458/93‑1 (MQ=255)

                                                      ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGtt                                       <  1:644980/93‑1 (MQ=255)

                                                            ggAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                 <  2:4590079/93‑1 (MQ=255)

                                                             gAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                >  1:2872117/1‑93 (MQ=255)

                                                             gAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                >  2:4528574/1‑93 (MQ=255)

                                                               acaCGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTca                              >  2:1169952/3‑93 (MQ=255)

                                                               aGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCAGGTCGTTCCCCATCACCTGCCAGCAGAGCATGGTTAATGCCTca                              >  1:1542814/1‑93 (MQ=255)

                                                                  cGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                           <  1:3568449/93‑1 (MQ=255)

                                                                  cGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                           <  1:1022727/93‑1 (MQ=255)

                                                                  cGTTCAGTTCAAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                           <  1:6111422/93‑1 (MQ=255)

                                                                  cGTTCAGTTCAAAGTTACCGGAAGCG‑‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                          >  2:6558657/1‑93 (MQ=255)

                                                                        gttcaAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGtt                     <  2:3110621/93‑1 (MQ=255)

                                                                          tcaAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                   >  2:1486288/1‑93 (MQ=255)

                                                                          tcaAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                   >  1:7538188/1‑93 (MQ=255)

                                                                           caAAGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                  <  2:4722073/93‑1 (MQ=255)

                                                                            aaaGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                 <  1:1874931/93‑1 (MQ=255)

                                                                            aaaGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                 >  2:823186/1‑93 (MQ=255)

                                                                            aaaGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                 >  2:7672310/1‑93 (MQ=255)

                                                                             aaGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                <  2:4951038/93‑1 (MQ=255)

                                                                             aaGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                <  2:5853411/93‑1 (MQ=255)

                                                                             aaGTTACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                <  1:2956624/93‑1 (MQ=255)

                                                                                ttACCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCAc             <  1:6284254/93‑1 (MQ=255)

                                                                                ttAACGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCAc             <  2:8753720/93‑1 (MQ=255)

                                                                                  aCCGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACtt           >  1:6739741/1‑93 (MQ=255)

                                                                                  aCCGGAAGCG‑‑CC‑CACCCATGTTTATCGCCACGTCGGTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACtt           <  2:6334444/93‑1 (MQ=255)

                                                                                   ccGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACttt          >  1:6558789/1‑93 (MQ=255)

                                                                                    cGGAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc         <  2:1835397/93‑1 (MQ=255)

                                                                                     ggAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcc        >  1:3272809/1‑93 (MQ=255)

                                                                                     ggAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcc        <  2:5358566/93‑1 (MQ=255)

                                                                                      gAAGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc       <  1:8462733/93‑1 (MQ=255)

                                                                                       aaGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcccc      >  1:467557/1‑93 (MQ=255)

                                                                                       aaGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcccc      <  1:4139780/93‑1 (MQ=255)

                                                                                       aaGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcccc      <  2:8042072/93‑1 (MQ=255)

                                                                                       aaGCGC‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTAAATGCCTCACACTGTGTTGGGTTCACTTTcccc      >  1:7031949/1‑93 (MQ=255)

                                                                                        aGCG‑‑CCAC‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTCGGTTCACTTTCCCCg     >  2:8554100/1‑93 (MQ=255)

                                                                                          cg‑‑cC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCa  <  2:41160/93‑1 (MQ=255)

TTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CC‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCA  >  NC_000913/1683469‑1683651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGA  >  NC_000913/1683475‑1683658

TGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCca                                                                                            >  SRR4302541.211879/1‑92 (MQ=60)

 GCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCcat                                                                                           >  SRR4302541.2187472/1‑91 (MQ=60)

   CATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGT                                                                                          >  SRR4302541.3214939/1‑94 (MQ=60)

   CATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGT                                                                                          <  SRR4302541.8695212/94‑1 (MQ=60)

   CATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGT                                                                                          >  SRR4302541.6872060/1‑94 (MQ=60)

   CATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGT                                                                                          >  SRR4302541.4296217/1‑94 (MQ=60)

   CATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGT                                                                                          >  SRR4302541.4451788/1‑94 (MQ=60)

   CATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGT                                                                                          >  SRR4302541.4461603/1‑94 (MQ=60)

    ATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCCATGT                                                                                          >  SRR4302541.6142662/1‑94 (MQ=60)

    ATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTT                                                                                         >  SRR4302541.3071168/1‑94 (MQ=60)

    ATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTT                                                                                         <  SRR4302541.3983950/94‑1 (MQ=60)

    ATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTT                                                                                         >  SRR4302541.2546543/1‑94 (MQ=60)

    ATCCGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTT                                                                                         >  SRR4302541.5621249/1‑94 (MQ=60)

      CTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGA                                                                                       >  SRR4302541.4102483/1‑94 (MQ=60)

      CTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGA                                                                                       <  SRR4302541.4768801/94‑1 (MQ=60)

       TGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGAT                                                                                      >  SRR4302541.5148005/1‑94 (MQ=60)

       TGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGAT                                                                                      >  SRR4302541.75066/1‑94 (MQ=60)

       TGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGAT                                                                                      <  SRR4302541.7539670/94‑1 (MQ=60)

          CAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGC                                                                                   >  SRR4302541.1235189/1‑94 (MQ=60)

           AGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCC                                                                                  >  SRR4302541.5620951/1‑94 (MQ=60)

           AGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCC                                                                                  >  SRR4302541.5884126/1‑94 (MQ=60)

           AGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCC                                                                                  >  SRR4302541.637800/1‑94 (MQ=60)

            GCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCA                                                                                 >  SRR4302541.7502155/1‑94 (MQ=60)

             CAAGCTCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCAC                                                                                <  SRR4302541.3229710/94‑1 (MQ=60)

             CAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCAC                                                                                >  SRR4302541.6808891/1‑94 (MQ=60)

             CAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCAC                                                                                <  SRR4302541.3312246/94‑1 (MQ=60)

             CAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACG                                                                               <  SRR4302541.406390/94‑1 (MQ=60)

              AAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACG                                                                               >  SRR4302541.1154171/1‑94 (MQ=60)

              AAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACG                                                                               >  SRR4302541.946225/1‑94 (MQ=60)

               AGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGT                                                                              <  SRR4302541.4807646/94‑1 (MQ=60)

                GCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTC                                                                             <  SRR4302541.7743045/94‑1 (MQ=60)

                GCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTC                                                                             <  SRR4302541.3745998/94‑1 (MQ=60)

                 CGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCG                                                                            <  SRR4302541.2091905/94‑1 (MQ=60)

                 CGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCG                                                                            <  SRR4302541.4391648/94‑1 (MQ=60)

                  GCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGT                                                                           >  SRR4302541.4801631/1‑94 (MQ=60)

                  GCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGT                                                                           >  SRR4302541.5409363/1‑94 (MQ=60)

                  GCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGT                                                                           >  SRR4302541.5584755/1‑94 (MQ=60)

                  GCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGT                                                                           <  SRR4302541.8192035/94‑1 (MQ=60)

                   CACCGATTGCGGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTT                                                                          <  SRR4302541.4764815/94‑1 (MQ=60)

                     CCGATTGCAGGAAATTGTGGATCACCATGGGACGGAAGACGTTCAATTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCC                                                                       <  SRR4302541.8451035/94‑1 (MQ=60)

                      CGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCC                                                                       >  SRR4302541.6117264/1‑94 (MQ=60)

                       GATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCC                                                                      <  SRR4302541.8670137/94‑1 (MQ=60)

                        ATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCA                                                                     >  SRR4302541.2933504/1‑94 (MQ=60)

                            CAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCCTGTTGATCGCCACGTCGTTCCCCATCAC                                                                 >  SRR4302541.3090460/1‑94 (MQ=60)

                              GGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCT                                                               >  SRR4302541.3168453/1‑94 (MQ=60)

                                AAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGA                                                             >  SRR4302541.2571206/1‑94 (MQ=60)

                                 AATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAC                                                            <  SRR4302541.140233/94‑1 (MQ=60)

                                  ATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACA                                                           >  SRR4302541.3725358/1‑94 (MQ=60)

                                   TTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAG                                                          >  SRR4302541.8903269/1‑94 (MQ=60)

                                     GTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCA                                                        >  SRR4302541.5514911/1‑94 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCCAAGTTACCGGAAGCGC‑CCCCCCATGTTTATCGCCACGTCGTTCCCCATCACCTGCCCGCAGAG                                                     >  SRR4302541.6007545/1‑94 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAG                                                     >  SRR4302541.3306997/1‑94 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAG                                                     <  SRR4302541.8802048/94‑1 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAG                                                     <  SRR4302541.2044779/94‑1 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAG                                                     <  SRR4302541.7911710/94‑1 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAG                                                     <  SRR4302541.6731228/94‑1 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAG                                                     <  SRR4302541.7148519/94‑1 (MQ=60)

                                        GATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGC                                                    >  SRR4302541.3953605/1‑94 (MQ=60)

                                          TCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCA                                                   <  SRR4302541.622394/94‑1 (MQ=60)

                                          TCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAT                                                  >  SRR4302541.3281721/1‑94 (MQ=60)

                                           CACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAT                                                  >  SRR4302541.4524857/1‑94 (MQ=60)

                                           CACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAT                                                  >  SRR4302541.1723360/1‑94 (MQ=60)

                                            ACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATG                                                 <  SRR4302541.295036/94‑1 (MQ=60)

                                            ACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCGCGTCGTTCCCCATCACCTGACAGCAGAGCATGG                                                >  SRR4302541.8559915/1‑94 (MQ=60)

                                             CCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGG                                                >  SRR4302541.207644/1‑94 (MQ=60)

                                             CCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGG                                                <  SRR4302541.8574558/94‑1 (MQ=60)

                                             CCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGG                                                <  SRR4302541.5785361/94‑1 (MQ=60)

                                             CCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGG                                                <  SRR4302541.3251222/94‑1 (MQ=60)

                                             CCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGG                                                <  SRR4302541.51420/94‑1 (MQ=60)

                                              CATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGT                                               >  SRR4302541.1667506/1‑94 (MQ=60)

                                               ATTGGGCGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGT                                               <  SRR4302541.1775222/94‑1 (MQ=60)

                                                TTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTA                                             <  SRR4302541.651073/94‑1 (MQ=60)

                                                TTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTA                                             <  SRR4302541.2727761/94‑1 (MQ=60)

                                                TTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTA                                             <  SRR4302541.6326203/94‑1 (MQ=60)

                                                  GGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAT                                           >  SRR4302541.7694541/1‑94 (MQ=60)

                                                   GACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATG                                          <  SRR4302541.8876282/94‑1 (MQ=60)

                                                    ACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGC                                         >  SRR4302541.3282098/1‑94 (MQ=60)

                                                      GGAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCT                                       >  SRR4302541.2900947/1‑94 (MQ=60)

                                                       GAAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTC                                      >  SRR4302541.3986473/1‑94 (MQ=60)

                                                        AAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCAGGTCGTTCCCCATCACCTGCCAGCAGAGCATGGTTAATGCCTCA                                     >  SRR4302541.1558303/1‑94 (MQ=60)

                                                        AAGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCC                                     >  SRR4302541.7379143/1‑94 (MQ=60)

                                                         AGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCAC                                    >  SRR4302541.2562733/1‑94 (MQ=60)

                                                         AGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCAC                                    >  SRR4302541.5819428/1‑94 (MQ=60)

                                                         AGACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCAC                                    >  SRR4302541.2440752/1‑94 (MQ=60)

                                                          GACGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACA                                   >  SRR4302541.4019131/1‑94 (MQ=60)

                                                            CGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACT                                 <  SRR4302541.3226936/94‑1 (MQ=60)

                                                            CGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACT                                 <  SRR4302541.1032177/94‑1 (MQ=60)

                                                            CGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACT                                 <  SRR4302541.3604592/94‑1 (MQ=60)

                                                            CGTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACT                                 <  SRR4302541.6173834/94‑1 (MQ=60)

                                                            CGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTG                                >  SRR4302541.6920140/1‑94 (MQ=60)

                                                             GTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTG                                <  SRR4302541.8170683/94‑1 (MQ=60)

                                                             GTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTG                                <  SRR4302541.6467277/94‑1 (MQ=60)

                                                             GTTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTG                                <  SRR4302541.3528640/94‑1 (MQ=60)

                                                              TTCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGT                               <  SRR4302541.3018242/94‑1 (MQ=60)

                                                               TCAGTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGCG                              >  SRR4302541.5303326/1‑94 (MQ=60)

                                                                  GTTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTG                           >  SRR4302541.2510007/1‑94 (MQ=60)

                                                                   TTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGG                          >  SRR4302541.7618762/1‑94 (MQ=60)

                                                                   TTCAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGG                          >  SRR4302541.2114631/1‑94 (MQ=60)

                                                                     CAAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGT                        <  SRR4302541.1474935/94‑1 (MQ=60)

                                                                     CAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTT                       >  SRR4302541.4729554/1‑94 (MQ=60)

                                                                     CAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTT                       <  SRR4302541.5414822/94‑1 (MQ=60)

                                                                      AAAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTT                       <  SRR4302541.1892964/94‑1 (MQ=60)

                                                                       AAGTTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTC                      <  SRR4302541.2986350/94‑1 (MQ=60)

                                                                          TTACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACT                   <  SRR4302541.6348448/94‑1 (MQ=60)

                                                                           TACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTT                  <  SRR4302541.4292593/94‑1 (MQ=60)

                                                                           TACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTT                  >  SRR4302541.6811686/1‑94 (MQ=60)

                                                                           TACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTT                 <  SRR4302541.3340170/94‑1 (MQ=60)

                                                                            ACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTT                 >  SRR4302541.6625843/1‑94 (MQ=60)

                                                                            ACCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTT                 >  SRR4302541.6625844/1‑94 (MQ=60)

                                                                             CCGGAAGCGCCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTT                 <  SRR4302541.552553/94‑1 (MQ=60)

                                                                             CCGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTC                >  SRR4302541.1626210/1‑94 (MQ=60)

                                                                              CGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCC               >  SRR4302541.3305821/1‑94 (MQ=60)

                                                                              CGGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACACCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCC               >  SRR4302541.2720087/1‑94 (MQ=60)

                                                                               GGAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCC              >  SRR4302541.3144721/1‑94 (MQ=60)

                                                                                GAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCC             <  SRR4302541.4760078/94‑1 (MQ=60)

                                                                                GAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCC             <  SRR4302541.8554021/94‑1 (MQ=60)

                                                                                GAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCC             <  SRR4302541.3848464/94‑1 (MQ=60)

                                                                                GAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCC             >  SRR4302541.472009/1‑94 (MQ=60)

                                                                                GAAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTAAATGCCTCACACTGTGTTGGGTTCACTTTCCCC             >  SRR4302541.7106704/1‑94 (MQ=60)

                                                                                 AAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCG            <  SRR4302541.4180684/94‑1 (MQ=60)

                                                                                 AAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCG            >  SRR4302541.2256634/1‑94 (MQ=60)

                                                                                 AAGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCG            >  SRR4302541.2774339/1‑94 (MQ=60)

                                                                                  AGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGG           <  SRR4302541.683568/94‑1 (MQ=60)

                                                                                  AGCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGG           <  SRR4302541.3959289/94‑1 (MQ=60)

                                                                                   GCGC‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGC          >  SRR4302541.5882622/1‑94 (MQ=60)

                                                                                     cgcCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCA         <  SRR4302541.2354106/91‑1 (MQ=60)

                                                                                     cgcCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCA         >  SRR4302541.5694928/4‑94 (MQ=60)

                                                                                     cgcCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCA         >  SRR4302541.7241403/4‑94 (MQ=60)

                                                                                     C‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATG       <  SRR4302541.3017182/94‑1 (MQ=60)

                                                                                     C‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATG       <  SRR4302541.1297615/94‑1 (MQ=60)

                                                                                     C‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATG       >  SRR4302541.3745989/1‑94 (MQ=60)

                                                                                     C‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATG       <  SRR4302541.3487533/94‑1 (MQ=60)

                                                                                      gcCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCAT        >  SRR4302541.3369046/3‑94 (MQ=60)

                                                                                        CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGT      >  SRR4302541.6852082/1‑94 (MQ=60)

                                                                                        CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGA      <  SRR4302541.7366519/94‑1 (MQ=60)

                                                                                         CCCCCATGTTGATCGCCACGTCGTTCCCCATCCCCTGACAGCAGAGCATGGTTAATACCTCACACTGAGTTGGGTTCACTTTCCCCGGCATGAT     >  SRR4302541.2703826/1‑94 (MQ=60)

                                                                                         CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGAT     >  SRR4302541.4784547/1‑94 (MQ=60)

                                                                                          CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATT    <  SRR4302541.8298136/94‑1 (MQ=60)

                                                                                          CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATT    <  SRR4302541.8877412/94‑1 (MQ=60)

                                                                                          CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTCTTCGGGTCACTTTACCCGGCATGATT    >  SRR4302541.7859814/1‑94 (MQ=60)

                                                                                           CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTG   >  SRR4302541.3268439/1‑94 (MQ=60)

                                                                                           CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGCCATGATTG   >  SRR4302541.8738701/1‑94 (MQ=60)

                                                                                            CCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGA  >  SRR4302541.8137108/1‑94 (MQ=60)

                                                                                            CCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGA  <  SRR4302541.1691935/94‑1 (MQ=60)

TGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGA  >  NC_000913/1683475‑1683658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`