Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,957,092 +CC intergenic (+26/+58) ilvC → / ← ppiC ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,0911.C94.0% 735.5 / 45.6 284intergenic (+25/+59)ilvC/ppiCketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base . (1/16);  new base C (26/241);  total (27/257)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*NC_0009133,957,0912.C94.0% 732.1 / 45.6 284intergenic (+25/+59)ilvC/ppiCketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base . (1/16);  new base C (26/241);  total (27/257)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 325 total aligned reads displayed.

TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑A‑‑C‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT  >  NC_000913/3956999‑3957173
                                                                                                ||                                                                                        
ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑a                                                                                            <  2:416138/93‑1 (MQ=255)
   aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑A‑‑ccc                                                                                       >  1:2276808/1‑91 (MQ=255)
   aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑A‑‑ccc                                                                                       <  2:3396574/93‑3 (MQ=255)
     cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑A‑‑C‑‑c‑‑ctc                                                                                 <  2:6233183/93‑1 (MQ=255)
       ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑‑C‑‑C‑‑c‑‑ctctc                                                                               >  1:2733040/1‑91 (MQ=39)
           ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCcac                                                                             <  1:5826047/93‑1 (MQ=38)
            gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCcaca                                                                            <  1:1461405/93‑1 (MQ=38)
            gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCcaca                                                                            <  2:5457041/93‑1 (MQ=38)
             taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCGCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACag                                                                           <  2:4871523/93‑1 (MQ=37)
             taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACag                                                                           <  2:6882380/93‑1 (MQ=37)
              aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACagg                                                                          <  2:7087050/93‑1 (MQ=37)
               aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACaggg                                                                         <  1:7046871/93‑1 (MQ=37)
               aGAAACTGCGCGGCTATAGGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACaggg                                                                         <  2:2837807/93‑1 (MQ=37)
                 aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCC‑C‑‑C‑‑T‑‑CTCCCACagggag                                                                       >  2:3020715/1‑93 (MQ=25)
                  aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACagggaga                                                                      <  1:1719211/93‑1 (MQ=37)
                  aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACagggaga                                                                      <  1:392174/93‑1 (MQ=37)
                   aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACagggagag                                                                     <  2:6011678/93‑1 (MQ=37)
                    cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACagggagagg                                                                    <  2:3957728/93‑1 (MQ=25)
                      gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACagggagaggga                                                                  <  2:2197818/93‑1 (MQ=25)
                        gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAgggagagggaga                                                                <  2:5132755/93‑1 (MQ=25)
                             tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGGGAGGGAGAAAaca                                                           >  1:1363061/1‑93 (MQ=21)
                             tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAaca                                                           <  2:2326069/93‑1 (MQ=21)
                             tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑‑ACCC‑‑T‑‑CTCCCCCACGGAGAGGGAGGAAaca                                                           >  1:4418928/1‑93 (MQ=21)
                              atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAacac                                                          <  2:129043/93‑1 (MQ=21)
                               tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACt                                                         <  1:8809767/93‑1 (MQ=21)
                                atGACAGATATGAATCGTATTGCTGTTGAGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTc                                                        <  1:3559358/93‑1 (MQ=18)
                                atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTc                                                        <  1:5194721/93‑1 (MQ=21)
                                 tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCC‑‑‑ACCC‑‑C‑‑CTCCCCCCGGGAGGGGGAGAAAAAAcac‑a                                                      >  1:4222671/1‑93 (MQ=21)
                                 tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGGGAAAACACTC‑a                                                      >  2:1518190/1‑93 (MQ=21)
                                 tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑a                                                      <  1:5630665/93‑1 (MQ=21)
                                 tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑a                                                      <  1:5724714/93‑1 (MQ=21)
                                  gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑aa                                                     <  2:4429771/93‑1 (MQ=21)
                                  gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑aa                                                     <  1:894070/93‑1 (MQ=21)
                                   aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑g                                                   <  1:6662402/93‑1 (MQ=21)
                                   aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑g                                                   <  1:4681242/93‑1 (MQ=21)
                                   aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑g                                                   <  2:5521833/93‑1 (MQ=21)
                                      gATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑ggcc                                                <  2:2938767/93‑4 (MQ=21)
                                       atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑ggcct                                               <  2:166480/93‑5 (MQ=21)
                                       atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑ggcct                                               <  1:995486/93‑5 (MQ=21)
                                         atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTtc                                              <  1:5452262/93‑1 (MQ=18)
                                         atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTtc                                              <  2:7851172/93‑1 (MQ=18)
                                         atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTtc                                              <  1:1126468/93‑1 (MQ=18)
                                         atGAAACGTATTGCTGTTGCGGATTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTtc                                              <  2:1391428/93‑1 (MQ=18)
                                            aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTcc                                           <  1:119487/93‑1 (MQ=18)
                                            aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTcc                                           <  2:2448187/93‑1 (MQ=18)
                                             aaCGTATTGCTGTTGCGGGTTAAGTGCTCGCTGATGCCCTCGCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCt                                          <  2:3699910/93‑1 (MQ=18)
                                             aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCt                                          <  1:1551275/93‑1 (MQ=18)
                                             aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTACCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCt                                          <  1:3372674/93‑1 (MQ=18)
                                              aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCC‑C‑‑C‑‑T‑‑CTCCCACAGGGAGAGCGAGAAAACACTC‑AAGGCCTTCTCCTg                                         <  1:5934435/93‑1 (MQ=18)
                                              aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTg                                         <  1:7681497/93‑1 (MQ=18)
                                                gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGga                                       <  1:7911160/93‑1 (MQ=18)
                                                gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGga                                       <  1:7239352/93‑1 (MQ=18)
                                                gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGga                                       <  1:2012298/93‑1 (MQ=18)
                                                 tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGgag                                      <  1:6000293/93‑1 (MQ=18)
                                                 tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑C‑‑CTCCCCCCGGGAGAGGGGGAAAACACTC‑AAGGCCTTCTCCTGgag                                      >  1:5963737/1‑93 (MQ=18)
                                                  aTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGgaga                                     <  2:8477131/93‑1 (MQ=18)
                                                   ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGaa                                    <  1:7474172/93‑1 (MQ=18)
                                                    tGCTGTTGCGGGTTAGTTGCGCGCAGATCCCCTCCCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACCCTC‑AAGGCCTTGTCCTGGAGAAg                                   <  1:6410893/93‑1 (MQ=12)
                                                    tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAg                                   <  1:4178416/93‑1 (MQ=18)
                                                    tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAg                                   <  2:8357754/93‑1 (MQ=18)
                                                    tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑A‑‑C‑‑CCCCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAg                                   <  2:5289923/93‑1 (MQ=21)
                                                     gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAgg                                  <  2:7438012/93‑1 (MQ=18)
                                                     gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAgg                                  <  1:2617012/93‑1 (MQ=18)
                                                       tGTTGCGGGTTAAGTGCGCGCTGATGTCCTCCCCCCGCCCC‑‑C‑‑C‑‑CTCCCCCAGGGGGGGGGGGAAAAACATC‑AG‑GCCATGTACTGGAGAAGGcg                                >  2:8667422/1‑92 (MQ=255)
                                                       cgtcgCGGGTTGAGCGCGCGCTGGTGCCCTCCCCCGG‑‑‑A‑‑CGGT‑‑CGCCCCCAGGGAGAGGGAGAAAACTCTC‑AAGGCGGTCTCCGGGAGAAGGcc                                <  2:1261663/89‑1 (MQ=9)
                                                        gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCt                               <  1:7560202/93‑1 (MQ=18)
                                                         ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑‑ACCC‑‑C‑‑CTCCCCCACGGAGGGGGGGAAAAAACTCAAA‑GCCTTCTCCTGGAGAAGGGCtt                              >  2:7220414/1‑93 (MQ=16)
                                                              ggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                         <  2:1816290/93‑1 (MQ=18)
                                                                ttAAGTGCGCGCTGATGCCCTCACCCCGCC‑C‑‑C‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                       <  1:3089720/93‑1 (MQ=18)
                                                                  aaGTGCGCGCTGATGGCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCCCGGGGAGAGGGAGAAAACAATC‑AAGGGCCTCTACTGGAGAAGGCCTTGCTATTAGt                     >  2:3057309/1‑93 (MQ=12)
                                                                  aaGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt                     <  1:6191010/93‑1 (MQ=18)
                                                                  aaGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt                     <  2:8775408/93‑1 (MQ=18)
                                                                  aaGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt                     <  2:6126222/93‑1 (MQ=18)
                                                                   aGTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt                    <  1:6328566/93‑1 (MQ=18)
                                                                    gTGCGCGCTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                   <  1:2478700/93‑1 (MQ=18)
                                                                      gcgcgcTGATGCCCTCCCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                 <  1:4062273/93‑1 (MQ=21)
                                                                       cgcgcTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                <  1:3721547/93‑1 (MQ=21)
                                                                        gcgcTGATGCCCTCCCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt               <  1:6760860/93‑1 (MQ=21)
                                                                          gcTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc             <  1:4540303/93‑1 (MQ=21)
                                                                          gcTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc             <  2:792120/93‑1 (MQ=21)
                                                                          gcTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc             <  1:7608668/93‑1 (MQ=21)
                                                                          gcTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc             <  2:4588967/93‑1 (MQ=21)
                                                                          gcTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACCCAAA‑GCCCTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc             >  2:7576633/1‑93 (MQ=18)
                                                                           cTGATGCCCTCCCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa            <  2:577530/93‑1 (MQ=21)
                                                                           cTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa            <  1:2835850/93‑1 (MQ=21)
                                                                           cTGATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa            <  1:689705/93‑1 (MQ=21)
                                                                             gATGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc          <  2:1322408/93‑1 (MQ=25)
                                                                               tGCCCTCGCCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc        <  1:6249037/93‑1 (MQ=25)
                                                                               tGCCCTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc        <  1:4905634/93‑1 (MQ=25)
                                                                               atcccTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGTCCTTGCTATTAGTTGCGGTACAGCAc        <  2:7043881/91‑1 (MQ=25)
                                                                                 cccTCACCCCGCC‑C‑‑C‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt      <  2:6533556/93‑1 (MQ=25)
                                                                                 cccTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt      <  2:495042/93‑1 (MQ=25)
                                                                                 cccTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt      <  1:7259317/93‑1 (MQ=25)
                                                                                   cTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCCCAGGGGGAGGGGGAAAACAATC‑AAGGCCTTCTCCTGGAGAAGGGCTTGCTATTAGTTGCGGGACAGCACCTTa    >  2:2097231/1‑93 (MQ=255)
                                                                                   cTCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTa    >  1:839994/1‑93 (MQ=37)
                                                                                    tCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa   <  1:7374503/93‑1 (MQ=37)
                                                                                    tCACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa   >  1:8613684/1‑93 (MQ=37)
                                                                                     cACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat  <  2:3335861/93‑1 (MQ=37)
                                                                                     cACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat  <  2:6702259/93‑1 (MQ=37)
                                                                                     cACCCCG‑‑‑ACCC‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat  >  2:4599892/1‑93 (MQ=37)
                                                                                                ||                                                                                        
TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑‑A‑‑C‑‑T‑‑CTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT  >  NC_000913/3956999‑3957173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 211 total aligned reads displayed.
TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC  >  NC_000913/3956999‑3957184
                                                                                                 |                                                                                              
TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑C                                                                                                <  SRR4302541.388605/94‑1 (MQ=60)
  GAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CCC                                                                                              >  SRR4302541.2298827/1‑94 (MQ=60)
   AGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CCCT                                                                                             <  SRR4302541.1935223/94‑1 (MQ=60)
    GCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CCCTC                                                                                            <  SRR4302541.6400567/94‑1 (MQ=60)
    GCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCATCACCCCG‑‑A‑‑CCCTC                                                                                            <  SRR4302541.8449885/94‑1 (MQ=60)
       GGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCC                                                                                           <  SRR4302541.195478/94‑1 (MQ=60)
       GGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCC                                                                                           <  SRR4302541.7011321/94‑1 (MQ=60)
        GTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCC                                                                                          <  SRR4302541.66745/94‑1 (MQ=60)
         TAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCA                                                                                         <  SRR4302541.5833069/94‑1 (MQ=60)
          AGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCAC                                                                                        <  SRR4302541.4674095/94‑1 (MQ=60)
          AGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCAC                                                                                        <  SRR4302541.3367643/94‑1 (MQ=60)
           GGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACA                                                                                       <  SRR4302541.5885288/94‑1 (MQ=60)
            GTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAG                                                                                      <  SRR4302541.1475699/94‑1 (MQ=60)
            GTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAG                                                                                      <  SRR4302541.3128576/94‑1 (MQ=60)
            GTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAG                                                                                      <  SRR4302541.7253708/94‑1 (MQ=60)
             TAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGG                                                                                     <  SRR4302541.6944817/94‑1 (MQ=60)
             TAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGG                                                                                     <  SRR4302541.755045/94‑1 (MQ=60)
               AGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGA                                                                                   <  SRR4302541.7190267/94‑1 (MQ=60)
               AGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGA                                                                                   <  SRR4302541.6157266/94‑1 (MQ=60)
               AGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGA                                                                                   <  SRR4302541.7121808/94‑1 (MQ=60)
               AGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGA                                                                                   <  SRR4302541.5031033/94‑1 (MQ=60)
               AGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGA                                                                                   <  SRR4302541.8276709/94‑1 (MQ=60)
                GAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAG                                                                                  <  SRR4302541.4298846/94‑1 (MQ=60)
                GAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGTAG                                                                                  <  SRR4302541.6036616/94‑1 (MQ=60)
                GAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAG                                                                                  <  SRR4302541.2924610/94‑1 (MQ=60)
                GAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAG                                                                                  <  SRR4302541.4136525/94‑1 (MQ=60)
                GAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAG                                                                                  <  SRR4302541.4021915/94‑1 (MQ=60)
                GAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAG                                                                                  <  SRR4302541.4008546/94‑1 (MQ=60)
                 AAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA                                                                                 <  SRR4302541.7945716/94‑1 (MQ=60)
                  AACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAG                                                                                <  SRR4302541.1736025/94‑1 (MQ=60)
                  AACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAG                                                                                <  SRR4302541.4334426/94‑1 (MQ=60)
                  AACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAG                                                                                <  SRR4302541.5802793/94‑1 (MQ=60)
                  AACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAG                                                                                <  SRR4302541.395806/94‑1 (MQ=60)
                    CTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCT‑‑ACCCTCTCCCACGGGGAGAGGG                                                                              <  SRR4302541.3586873/94‑1 (MQ=60)
                    CTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGG                                                                              <  SRR4302541.72333/94‑1 (MQ=60)
                    CTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGG                                                                              <  SRR4302541.2412230/94‑1 (MQ=60)
                      GCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAG                                                                            <  SRR4302541.3147268/94‑1 (MQ=60)
                      GCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAG                                                                            <  SRR4302541.163473/94‑1 (MQ=60)
                        GCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAA                                                                          <  SRR4302541.3661673/94‑1 (MQ=60)
                          GGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGGGAAAA                                                                        >  SRR4302541.1461423/1‑94 (MQ=60)
                           GCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAAC                                                                       <  SRR4302541.7324777/94‑1 (MQ=60)
                            CTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGGGAGGGAGAAAACA                                                                      >  SRR4302541.1376497/1‑94 (MQ=60)
                            CTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑ACCCTCTCCCCCACGGAGAGGGAGGAAACA                                                                      >  SRR4302541.4463270/1‑94 (MQ=60)
                             TATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCGCTCACCCCG‑‑A‑‑Cccccgcccccccggacggggggggaaaaa                                                                   >  SRR4302541.8517180/1‑65 (MQ=60)
                             TATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACAC                                                                     <  SRR4302541.3821956/94‑1 (MQ=60)
                               TATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC                                                                   <  SRR4302541.8903839/94‑1 (MQ=60)
                                ATGACAGATATGAATCGTATTGCTGTTGAGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A                                                                 <  SRR4302541.3595376/94‑1 (MQ=57)
                                ATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A                                                                 <  SRR4302541.5248180/94‑1 (MQ=60)
                                 TGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA                                                                <  SRR4302541.5688262/94‑1 (MQ=60)
                                 TGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA                                                                <  SRR4302541.5783075/94‑1 (MQ=60)
                                 TGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA                                                                <  SRR4302541.7435597/94‑1 (MQ=60)
                                 TGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA                                                                <  SRR4302541.152718/94‑1 (MQ=60)
                                  GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCGCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                              <  SRR4302541.1248178/94‑1 (MQ=60)
                                  GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AA‑G                                                              <  SRR4302541.5324136/94‑1 (MQ=60)
                                  GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                              <  SRR4302541.902297/94‑1 (MQ=60)
                                  GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                              <  SRR4302541.906802/94‑1 (MQ=60)
                                  GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                              <  SRR4302541.8100555/94‑1 (MQ=60)
                                  GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                              <  SRR4302541.906803/94‑1 (MQ=60)
                                   ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AA‑GG                                                             <  SRR4302541.6275750/94‑1 (MQ=60)
                                   ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GG                                                             <  SRR4302541.6730762/94‑1 (MQ=60)
                                   ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GG                                                             <  SRR4302541.4728919/94‑1 (MQ=60)
                                    CAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GGC                                                            <  SRR4302541.6024087/94‑1 (MQ=60)
                                       ATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTT                                                          <  SRR4302541.1004747/94‑1 (MQ=60)
                                       ATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTT                                                          <  SRR4302541.2988182/94‑1 (MQ=60)
                                         ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                        <  SRR4302541.1137098/94‑1 (MQ=60)
                                         ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                        <  SRR4302541.2276866/94‑1 (MQ=60)
                                         ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                        <  SRR4302541.5508203/94‑1 (MQ=60)
                                         ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                        <  SRR4302541.1430421/94‑1 (MQ=60)
                                         ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                        <  SRR4302541.4920552/94‑1 (MQ=60)
                                         ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                        <  SRR4302541.7652071/94‑1 (MQ=60)
                                          TGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTC                                                       <  SRR4302541.2895246/94‑1 (MQ=60)
                                           GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCC                                                      <  SRR4302541.2070582/94‑1 (MQ=60)
                                           GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCC                                                      <  SRR4302541.6318827/94‑1 (MQ=60)
                                           GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCC                                                      <  SRR4302541.6453792/94‑1 (MQ=60)
                                           GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCC                                                      <  SRR4302541.7544/94‑1 (MQ=60)
                                            AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCT                                                     <  SRR4302541.8700810/94‑1 (MQ=60)
                                            AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCT                                                     <  SRR4302541.120438/94‑1 (MQ=60)
                                            AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCT                                                     <  SRR4302541.7686941/94‑1 (MQ=60)
                                            AAACGTATGGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCGCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCT                                                     <  SRR4302541.899251/94‑1 (MQ=47)
                                             AACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTG                                                    <  SRR4302541.1566831/94‑1 (MQ=60)
                                             AACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTACCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTG                                                    <  SRR4302541.3406306/94‑1 (MQ=60)
                                             AACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCACCCACCGGGCGAGGGGGAAAACAATC‑AAGGACTTCTCCTG                                                    >  SRR4302541.2749479/1‑94 (MQ=37)
                                              ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGCGAGAAAACACTC‑AAGGCCTTCTCCTGG                                                   <  SRR4302541.5994885/94‑1 (MQ=47)
                                              ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGG                                                   <  SRR4302541.7763157/94‑1 (MQ=60)
                                              ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGG                                                   <  SRR4302541.2779411/94‑1 (MQ=60)
                                                GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAG                                                 <  SRR4302541.7998201/94‑1 (MQ=60)
                                                GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAG                                                 <  SRR4302541.7316558/94‑1 (MQ=60)
                                                GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAG                                                 <  SRR4302541.2031785/94‑1 (MQ=60)
                                                GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAG                                                 <  SRR4302541.7316557/94‑1 (MQ=60)
                                                GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCCCTCCCCCCGGGAGAGGGGGAAAACACTC‑AAGGCCTTCTCCTGGAG                                                 >  SRR4302541.6024523/1‑94 (MQ=52)
                                                 TATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGA                                                <  SRR4302541.6061759/94‑1 (MQ=60)
                                                  ATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAA                                               <  SRR4302541.5652214/94‑1 (MQ=60)
                                                   TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                              <  SRR4302541.8862739/94‑1 (MQ=60)
                                                   TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                              <  SRR4302541.7554121/94‑1 (MQ=60)
                                                   TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                              <  SRR4302541.6085729/94‑1 (MQ=60)
                                                   TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑Ccctctccccccgggaggggggggaaaaaaccaaagcctcctcctgggaacg                                             >  SRR4302541.6172175/1‑43 (MQ=60)
                                                    TGCTGTTGCGGGTTAGTTGCGCGCAGATCCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACCCTC‑AAGGCCTTGTCCTGGAGAAGG                                             <  SRR4302541.6476277/94‑1 (MQ=13)
                                                    TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGG                                             <  SRR4302541.4219859/94‑1 (MQ=60)
                                                    TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGG                                             <  SRR4302541.1185358/94‑1 (MQ=60)
                                                    TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGG                                             <  SRR4302541.6476279/94‑1 (MQ=60)
                                                    TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGG                                             <  SRR4302541.2816585/94‑1 (MQ=60)
                                                     GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGTCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGC                                            <  SRR4302541.4478876/94‑1 (MQ=49)
                                                     GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGC                                            <  SRR4302541.7617963/94‑1 (MQ=60)
                                                     GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGC                                            <  SRR4302541.2643149/94‑1 (MQ=60)
                                                     GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑ACCCTCTCCCACAGGGAGAGGGGGAAAACACTCAAA‑GCCTTCTCCTCGAGAAGGC                                            >  SRR4302541.1480779/1‑94 (MQ=45)
                                                      CTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCC                                           <  SRR4302541.4699923/94‑1 (MQ=60)
                                                       TGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCT                                          <  SRR4302541.7537097/94‑1 (MQ=60)
                                                       TGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCT                                          <  SRR4302541.6930083/94‑1 (MQ=60)
                                                       CGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCT                                          <  SRR4302541.5646958/94‑1 (MQ=60)
                                                        GTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTT                                         <  SRR4302541.7640995/94‑1 (MQ=60)
                                                         TTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTG                                        <  SRR4302541.2325009/94‑1 (MQ=60)
                                                         TTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTG                                        <  SRR4302541.7010772/94‑1 (MQ=60)
                                                          TGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGC                                       <  SRR4302541.6320419/94‑1 (MQ=60)
                                                            CGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CCCTCCCACAGGGAGAGGGAGGAAAAACTC‑AACGCCTTCTCCTGGAGAAGGCCCTGCTT                                     >  SRR4302541.5904319/1‑94 (MQ=21)
                                                             gcggggtaaggtccccccggatcccctccccccaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCT                                      <  SRR4302541.4400611/58‑1 (MQ=60)
                                                             GGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTAT                                    <  SRR4302541.8579872/94‑1 (MQ=60)
                                                             GGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTAT                                    <  SRR4302541.5784832/94‑1 (MQ=60)
                                                               GTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTA                                  <  SRR4302541.6625119/94‑1 (MQ=60)
                                                                TTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAG                                 <  SRR4302541.3121057/94‑1 (MQ=49)
                                                                TTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAG                                 <  SRR4302541.2511607/94‑1 (MQ=60)
                                                                  AAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTT                               <  SRR4302541.6254275/94‑1 (MQ=60)
                                                                   AGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTG                              <  SRR4302541.6393269/94‑1 (MQ=60)
                                                                   AGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAAAACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTG                              <  SRR4302541.6045166/94‑1 (MQ=52)
                                                                    GTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGC                             <  SRR4302541.2734736/94‑1 (MQ=60)
                                                                    GTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGATGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGC                             >  SRR4302541.7517516/1‑94 (MQ=50)
                                                                    GTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGC                             <  SRR4302541.2503555/94‑1 (MQ=60)
                                                                      GCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGG                           <  SRR4302541.4102366/94‑1 (MQ=60)
                                                                      GCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGG                           <  SRR4302541.2352052/94‑1 (MQ=60)
                                                                      GCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGG                           <  SRR4302541.4102368/94‑1 (MQ=60)
                                                                       CGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGT                          <  SRR4302541.5980132/94‑1 (MQ=60)
                                                                       CGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGT                          <  SRR4302541.3759057/94‑1 (MQ=60)
                                                                        GCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                         <  SRR4302541.6833158/94‑1 (MQ=60)
                                                                        GCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                         <  SRR4302541.1692941/94‑1 (MQ=60)
                                                                        GCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                         <  SRR4302541.8743319/94‑1 (MQ=60)
                                                                         CGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAC                        <  SRR4302541.3719733/94‑1 (MQ=60)
                                                                          GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                       <  SRR4302541.7689973/94‑1 (MQ=60)
                                                                          GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                       <  SRR4302541.4586195/94‑1 (MQ=60)
                                                                          GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                       <  SRR4302541.786188/94‑1 (MQ=60)
                                                                           CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                      <  SRR4302541.2864394/94‑1 (MQ=60)
                                                                           CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                      <  SRR4302541.696199/94‑1 (MQ=60)
                                                                            TGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                     <  SRR4302541.6312317/94‑1 (MQ=60)
                                                                            TGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                     <  SRR4302541.5235228/94‑1 (MQ=60)
                                                                               TGCCCTCGCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                  <  SRR4302541.6312877/94‑1 (MQ=60)
                                                                               TGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                  <  SRR4302541.4955681/94‑1 (MQ=60)
                                                                               TGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                  <  SRR4302541.4302363/94‑1 (MQ=60)
                                                                               TGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                  <  SRR4302541.3816771/94‑1 (MQ=60)
                                                                                GCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCT                 <  SRR4302541.5062429/94‑1 (MQ=60)
                                                                                 CCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                <  SRR4302541.1235232/94‑1 (MQ=60)
                                                                                 CCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                <  SRR4302541.7336756/94‑1 (MQ=60)
                                                                                  CCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA               >  SRR4302541.893588/1‑94 (MQ=60)
                                                                                  CCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA               >  SRR4302541.847611/1‑94 (MQ=60)
                                                                                  CCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA               <  SRR4302541.6801813/94‑1 (MQ=60)
                                                                                   CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGTAACTTAA              >  SRR4302541.4253689/1‑94 (MQ=60)
                                                                                   CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA              >  SRR4302541.8706049/1‑94 (MQ=60)
                                                                                   CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA              <  SRR4302541.6731197/94‑1 (MQ=60)
                                                                                   CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA              >  SRR4302541.5364847/1‑94 (MQ=60)
                                                                                    TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTGTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT             >  SRR4302541.4544586/1‑94 (MQ=60)
                                                                                    TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT             <  SRR4302541.7453328/94‑1 (MQ=60)
                                                                                    TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT             <  SRR4302541.7741799/94‑1 (MQ=60)
                                                                                     CACCCCG‑‑ACCCTCTCCCACAGGGGGAGGGAGAAAACACTCAAA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG            >  SRR4302541.3628026/1‑94 (MQ=60)
                                                                                     CACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACAATC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG            >  SRR4302541.2000348/1‑94 (MQ=60)
                                                                                      ACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA           >  SRR4302541.6167682/1‑94 (MQ=60)
                                                                                       CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTActgct          >  SRR4302541.4597086/1‑89 (MQ=60)
                                                                                       CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT          >  SRR4302541.4597087/1‑94 (MQ=60)
                                                                                       CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT          <  SRR4302541.4764290/94‑1 (MQ=60)
                                                                                       CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT          >  SRR4302541.6659317/1‑94 (MQ=60)
                                                                                        cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCTTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA           <  SRR4302541.2853937/85‑1 (MQ=60)
                                                                                        cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA           <  SRR4302541.360408/85‑1 (MQ=60)
                                                                                        cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA           <  SRR4302541.2109238/85‑1 (MQ=60)
                                                                                        cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA           <  SRR4302541.8448566/85‑1 (MQ=60)
                                                                                        CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG         >  SRR4302541.7847217/1‑94 (MQ=60)
                                                                                        CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG         >  SRR4302541.8856836/1‑94 (MQ=60)
                                                                                        CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG         <  SRR4302541.6193187/94‑1 (MQ=60)
                                                                                         CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT        >  SRR4302541.2331216/1‑94 (MQ=60)
                                                                                         CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT        >  SRR4302541.1151706/1‑94 (MQ=60)
                                                                                         CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT        <  SRR4302541.5738462/94‑1 (MQ=60)
                                                                                         CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT        <  SRR4302541.5577235/94‑1 (MQ=60)
                                                                                            cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG       >  SRR4302541.1613918/6‑94 (MQ=60)
                                                                                            cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG       <  SRR4302541.2102475/89‑1 (MQ=60)
                                                                                            cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG       >  SRR4302541.6795447/6‑94 (MQ=60)
                                                                                            cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG       >  SRR4302541.4343062/6‑94 (MQ=60)
                                                                                             gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA      >  SRR4302541.2470044/5‑94 (MQ=60)
                                                                                             gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA      >  SRR4302541.1727015/5‑94 (MQ=60)
                                                                                             gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA      <  SRR4302541.4709492/90‑1 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     <  SRR4302541.3027814/91‑1 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     <  SRR4302541.6247723/91‑1 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     <  SRR4302541.2249576/91‑1 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     >  SRR4302541.5144739/4‑94 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     >  SRR4302541.3358031/4‑94 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     >  SRR4302541.3199953/4‑94 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     >  SRR4302541.7190769/4‑94 (MQ=60)
                                                                                              accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT     <  SRR4302541.4610988/91‑1 (MQ=60)
                                                                                              C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT   >  SRR4302541.5473153/1‑94 (MQ=60)
                                                                                              C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT   >  SRR4302541.7152971/1‑94 (MQ=60)
                                                                                              C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT   <  SRR4302541.4871365/94‑1 (MQ=60)
                                                                                              C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGCTACAGCACCTTAATGATGTGATAT   <  SRR4302541.2143925/94‑1 (MQ=60)
                                                                                                 CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCATCTTAATGATGTGATATC  >  SRR4302541.6638924/1‑94 (MQ=60)
                                                                                                 CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC  <  SRR4302541.5645218/94‑1 (MQ=60)
                                                                                                 CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC  <  SRR4302541.3637781/94‑1 (MQ=60)
                                                                                                 CTCGCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC  <  SRR4302541.5076625/94‑1 (MQ=60)
                                                                                                 |                                                                                              
TTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC  >  NC_000913/3956999‑3957184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑