Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,705,052 T→C V37A (GTA→GCA)  b1629 → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,705,0520TC100.0% 527.5 / NA 159V37A (GTA→GCA) b1629putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/109);  total (50/109)

Only 100 of 196 total aligned reads displayed.

CATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGC  >  NC_000913/1704962‑1705141
                                                                                           |                                                                                         
  ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCCGGCAccc                                                                                       >  2:419380/1‑93 (MQ=255)
  ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCAccc                                                                                       <  1:3889752/93‑1 (MQ=255)
  ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCAccc                                                                                       <  2:1041254/93‑1 (MQ=255)
   tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACTGTACACCCCT‑GCGCCAGGCAcccc                                                                                      <  1:2181534/93‑1 (MQ=255)
   tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCAcccc                                                                                      <  2:4970668/93‑1 (MQ=255)
   tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCAcccc                                                                                      <  1:6402673/93‑1 (MQ=255)
    cAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCt                                                                                     <  2:3315390/93‑1 (MQ=255)
    cAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCt                                                                                     <  1:2676742/93‑1 (MQ=255)
     aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTg                                                                                    <  2:1053069/93‑1 (MQ=255)
     aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTg                                                                                    <  1:6223588/93‑1 (MQ=255)
      gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGGACACCCCT‑GCGCCAGGCACCCCTgg                                                                                   >  2:2132510/1‑93 (MQ=255)
       aaaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCCGgc                                                                                  >  1:3767691/1‑93 (MQ=255)
        aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCCCCCCTGgcc                                                                                 >  1:1045212/1‑92 (MQ=255)
         aaaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCCGgcgc                                                                                >  2:3926221/1‑93 (MQ=255)
          aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCa                                                                               <  1:4150949/93‑1 (MQ=255)
           aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGgcgccg                                                                              >  2:4620349/1‑91 (MQ=255)
           aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAg                                                                              >  2:606255/1‑93 (MQ=255)
           aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAg                                                                              <  1:6072679/93‑1 (MQ=255)
            ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGc                                                                             <  1:1684076/93‑1 (MQ=255)
                aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAGCCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGttt                                                                         <  2:1176170/93‑1 (MQ=255)
                aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCCGCGttt                                                                         >  2:5292251/1‑93 (MQ=255)
                aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGttt                                                                         >  1:4066527/1‑93 (MQ=255)
                aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGttt                                                                         <  2:4864817/93‑1 (MQ=255)
                aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGttt                                                                         >  2:2897004/1‑93 (MQ=255)
                 atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCCGGCACCCCCGGCGCAGCGgttt                                                                        >  1:3674162/1‑93 (MQ=255)
                   cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGt                                                                      <  2:2279200/93‑1 (MQ=255)
                    tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGGCCGGCACCCCCGGGGCAGCGGTTTGtt                                                                     >  1:6290261/1‑93 (MQ=255)
                     gggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTa                                                                    >  2:822418/1‑93 (MQ=255)
                      ggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTAt                                                                   >  1:3250520/1‑93 (MQ=255)
                        aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGGCCCAAGGGACCCCT‑GCGCGCCGttttttttttt                                                                 >  2:456126/1‑92 (MQ=255)
                        aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTc                                                                 >  2:5210001/1‑93 (MQ=255)
                         tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTcc                                                                <  2:323513/93‑1 (MQ=255)
                         tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTcc                                                                <  1:5551015/93‑1 (MQ=255)
                         tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCCCGCGCAGCGGTtttttttttc                                                                >  1:5758332/1‑91 (MQ=255)
                           tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCCGGCACCCCCGGCGCCGCGGTTTTTTTTTTCAc                                                              >  2:3579100/1‑93 (MQ=255)
                           tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTcccc                                                              >  2:2706583/1‑91 (MQ=255)
                            cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACt                                                             >  2:653526/1‑93 (MQ=255)
                             aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGTCGCAGCGTTTTGTTATTCCACTg                                                            <  2:3865810/93‑1 (MQ=255)
                             aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                            <  2:726125/93‑1 (MQ=255)
                               cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAAGCACCCCCCTCCCGGCGTGTTGTTTTTTCAccgta                                                          >  2:3513373/1‑89 (MQ=255)
                                 gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAc                                                        >  1:5351705/1‑93 (MQ=255)
                                 gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAc                                                        >  2:3178910/1‑93 (MQ=255)
                                  cggcATCCATCCACCGTAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                       <  1:867483/93‑1 (MQ=255)
                                  cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                       <  1:4704421/93‑1 (MQ=255)
                                  cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                       <  1:4230816/93‑1 (MQ=255)
                                  cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                       <  2:5718276/93‑1 (MQ=255)
                                   ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCAACTGAAACAg                                                      >  1:950231/1‑93 (MQ=255)
                                    gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCCGGCGCAGCGGTTTTTTTTTTCACTTAAACAGc                                                     >  1:2603920/1‑93 (MQ=255)
                                     catccatcCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                    >  1:4344498/1‑93 (MQ=255)
                                     catccatcCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                    <  1:2283650/93‑1 (MQ=255)
                                      atccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGGCAGGCCCCCCTGGCGCCGCGTTTTGTTATTTCACTCAAACAGCat                                                   >  2:1435879/1‑93 (MQ=255)
                                        ccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCatat                                                 <  1:5920782/93‑1 (MQ=255)
                                        ccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACACCT‑GCGACAGGCAACCCCGGCGCTCCGGTTTGTTTTTCCACTGGAACAAAatat                                                 >  1:2018353/1‑93 (MQ=255)
                                         catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                <  2:4392851/93‑1 (MQ=255)
                                         catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                <  2:3798088/93‑1 (MQ=255)
                                         catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                <  1:3480747/93‑1 (MQ=255)
                                         catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                <  2:2004844/93‑1 (MQ=255)
                                         catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCCCCAGGGACCCCTGGCGCAGCGTTTTGTTATTCCACTGACACAGCATAtt                                                >  1:5108783/1‑93 (MQ=255)
                                          atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGTCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                               <  2:5907921/93‑1 (MQ=255)
                                          atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                               <  1:5162463/93‑1 (MQ=255)
                                          atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                               <  1:3956098/93‑1 (MQ=255)
                                          atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                               <  2:5701548/93‑1 (MQ=255)
                                           tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                              <  2:3286592/93‑1 (MQ=255)
                                           tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                              <  1:3157373/93‑1 (MQ=255)
                                           tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                              <  2:1636237/93‑1 (MQ=255)
                                               ccGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGGCAGGGACCCCCGGCGCAGCGGTTTGTTATTTCACTGAAACAGCATATTGGCGCt                                          >  2:403754/1‑93 (MQ=255)
                                                 ggAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGa                                        <  1:733454/93‑1 (MQ=255)
                                                   agaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAg                                      >  2:5796788/1‑93 (MQ=255)
                                                   agaTGAAAACCCAGTCCAACGGTACACCCCT‑GCCCCCGGCCCCCCCCCCGCCGCGGTTTGTTATTTCCCTTAAACAGCATATTTGGGCTGAAg                                      >  2:4819722/1‑93 (MQ=255)
                                                    gaTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCCGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                     >  2:1925779/1‑93 (MQ=255)
                                                     aTGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                    <  1:177877/93‑1 (MQ=255)
                                                      tGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                   <  2:1161312/93‑1 (MQ=255)
                                                      tGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                   <  2:615622/93‑1 (MQ=255)
                                                       gAAAACCCAGTCCAACGGTACACCCCT‑GCGCCCGGCACCCCCCGCGCAGCGTTTTGTTTTTGCACTGAAAAAACATATTTGCGCTGAAggggg                                  >  2:2592328/1‑90 (MQ=255)
                                                       gAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                  <  1:4611530/93‑1 (MQ=255)
                                                       gAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                  <  2:3118685/93‑1 (MQ=255)
                                                          aaCCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt                               >  2:5414933/1‑93 (MQ=255)
                                                          aaCCCAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt                               <  2:6456357/93‑1 (MQ=255)
                                                            accAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgt                             <  2:4107113/92‑1 (MQ=255)
                                                              cAGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCCGCGGTTTGATATTCCACTGAAACAGCATATTGGCGCTGAAGGTGCGGTGTGc                           >  1:2095698/1‑93 (MQ=255)
                                                              cAGGCCAACGGTTCCCCCCT‑GCGCCAGTCTCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTTAGTTGTGc                           <  1:1923469/93‑1 (MQ=255)
                                                               aGTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg                          <  2:2506525/93‑1 (MQ=255)
                                                                gTCCAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGt                         <  1:46347/93‑1 (MQ=255)
                                                                  ccAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTa                       <  1:3423473/93‑1 (MQ=255)
                                                                   cAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                      <  2:4489166/93‑1 (MQ=255)
                                                                   cAACGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                      <  1:1569998/93‑1 (MQ=255)
                                                                     aCGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                    <  2:1513419/93‑1 (MQ=255)
                                                                      cGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGt                   <  2:3743649/93‑1 (MQ=255)
                                                                      cGGTACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGt                   <  1:5633967/93‑1 (MQ=255)
                                                                         tACACCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCgg                <  2:4751508/93‑1 (MQ=255)
                                                                           cacCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCg              >  2:608086/1‑93 (MQ=255)
                                                                           cacCCCT‑GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCg              <  1:2041413/93‑1 (MQ=255)
                                                                                  ggcgcCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg        <  2:5377995/92‑1 (MQ=255)
                                                                                   gcgcCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt       >  1:1104156/1‑93 (MQ=255)
                                                                                       cAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg   <  2:2096513/93‑1 (MQ=255)
                                                                                        aGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc  >  2:1851549/1‑93 (MQ=255)
                                                                                        aGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc  >  2:123549/1‑93 (MQ=255)
                                                                                        aGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc  >  1:4811943/1‑93 (MQ=255)
                                                                                        aGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc  <  1:2987175/93‑1 (MQ=255)
                                                                                        aGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc  <  2:702511/93‑1 (MQ=255)
                                                                                           |                                                                                         
CATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCT‑GCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGC  >  NC_000913/1704962‑1705141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGG  >  NC_000913/1704959‑1705145
                                                                                             |                                                                                             
CTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGC                                                                                               >  SRR4302534.1133927/1‑94 (MQ=60)
  GCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCAC                                                                                             <  SRR4302534.746257/94‑1 (MQ=60)
   CATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGCACC                                                                                            >  SRR4302534.3090564/1‑94 (MQ=60)
     TTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCC                                                                                          <  SRR4302534.3994173/94‑1 (MQ=60)
      TCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACTGTACACCCCTGCGCCAGGCACCCCT                                                                                         <  SRR4302534.2239198/94‑1 (MQ=60)
      TCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCT                                                                                         <  SRR4302534.6057768/94‑1 (MQ=60)
      TCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCT                                                                                         <  SRR4302534.6575534/94‑1 (MQ=60)
       CAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTG                                                                                        <  SRR4302534.2747512/94‑1 (MQ=60)
       CAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGGACCCCTG                                                                                        >  SRR4302534.4225632/1‑94 (MQ=60)
        AGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCACCGGTACACCCCTGCGCCAGGCACCCCTGG                                                                                       <  SRR4302534.2731560/94‑1 (MQ=60)
        AGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGG                                                                                       <  SRR4302534.6392586/94‑1 (MQ=60)
         GAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGC                                                                                      >  SRR4302534.3868842/1‑94 (MQ=60)
          AAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCCCCCCTGGCC                                                                                     >  SRR4302534.1072222/1‑94 (MQ=60)
           AAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGC                                                                                    >  SRR4302534.3294969/1‑94 (MQ=60)
             AAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAG                                                                                  <  SRR4302534.4262998/94‑1 (MQ=60)
              AATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCccgcgccgc                                                                                 >  SRR4302534.4881712/1‑85 (MQ=60)
              AATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGC                                                                                 <  SRR4302534.6237792/94‑1 (MQ=60)
              AATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGC                                                                                 >  SRR4302534.916274/1‑94 (MQ=60)
               ATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCG                                                                                <  SRR4302534.1727877/94‑1 (MQ=60)
               ATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCG                                                                                <  SRR4302534.4396906/94‑1 (MQ=60)
                TAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGT                                                                               >  SRR4302534.6348212/1‑94 (MQ=60)
                TAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGT                                                                               >  SRR4302534.6612144/1‑94 (MQ=60)
                 AAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTT                                                                              <  SRR4302534.5171671/94‑1 (MQ=60)
                  AAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTT                                                                             >  SRR4302534.4175632/1‑94 (MQ=60)
                   AATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGCACCCCCGGCGCAGCGGTTT                                                                            >  SRR4302534.3772930/1‑94 (MQ=60)
                   AATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGCGCCGCGGTTT                                                                            >  SRR4302534.1818107/1‑94 (MQ=60)
                     TCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGCGCAGCGGTTTGT                                                                          >  SRR4302534.4062216/1‑94 (MQ=60)
                      CTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGGCCGGCACCCCCGGGGCAGCGGTTTGTT                                                                         >  SRR4302534.6460445/1‑94 (MQ=60)
                      CTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCTCCCCTGGCGCAGCGTTTTGTT                                                                         <  SRR4302534.4821387/94‑1 (MQ=60)
                      CTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTT                                                                         <  SRR4302534.6401168/94‑1 (MQ=60)
                        GGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTAT                                                                       >  SRR4302534.3337080/1‑94 (MQ=60)
                          gcatcCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTC                                                                     <  SRR4302534.4771941/89‑1 (MQ=60)
                           ATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCccgcgcagcggttttttttttc                                                                    >  SRR4302534.5914529/1‑72 (MQ=60)
                           ATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCC                                                                    <  SRR4302534.2183089/94‑1 (MQ=60)
                            TTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCA                                                                   <  SRR4302534.5700870/94‑1 (MQ=60)
                              TCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGGTTTGTTATTtccct                                                                 >  SRR4302534.2963872/1‑89 (MQ=60)
                               CAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTG                                                                >  SRR4302534.5329152/1‑94 (MQ=60)
                                AACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGA                                                               >  SRR4302534.5522893/1‑94 (MQ=60)
                                AACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGCGCCGCGGTTTGTTTTTTCACTGA                                                               >  SRR4302534.4055562/1‑94 (MQ=60)
                                   GGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAC                                                            >  SRR4302534.5496000/1‑94 (MQ=60)
                                    GCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACA                                                           <  SRR4302534.6534541/94‑1 (MQ=60)
                                     CGGCATCCATCCACCGTAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAG                                                          <  SRR4302534.889385/94‑1 (MQ=60)
                                     CGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAG                                                          <  SRR4302534.4344844/94‑1 (MQ=60)
                                     CGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAG                                                          <  SRR4302534.4832128/94‑1 (MQ=60)
                                     CGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCAACTGAAACAG                                                          >  SRR4302534.974430/1‑94 (MQ=60)
                                      GGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGCGCAGCGGTTTTTTTTTTCACTTAAACAGC                                                         >  SRR4302534.2672821/1‑94 (MQ=60)
                                       GCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCA                                                        >  SRR4302534.4461840/1‑94 (MQ=60)
                                        CATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAT                                                       <  SRR4302534.2344250/94‑1 (MQ=60)
                                        CATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGCGCAGCGTTTTGTTATTTCACTGAAACAGCAT                                                       >  SRR4302534.1711581/1‑94 (MQ=60)
                                           CCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATT                                                    <  SRR4302534.6081535/94‑1 (MQ=60)
                                           CCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCCCCAGGGACCCCTGGCGCAGCGTTTTGTTATTCCACTGACACAGCATATT                                                    >  SRR4302534.5246647/1‑94 (MQ=60)
                                            CATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTG                                                   <  SRR4302534.3574309/94‑1 (MQ=60)
                                             ATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGG                                                  <  SRR4302534.4062024/94‑1 (MQ=60)
                                             ATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGG                                                  <  SRR4302534.5301636/94‑1 (MQ=60)
                                             ATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGG                                                  <  SRR4302534.856095/94‑1 (MQ=60)
                                              TCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTTCACTGAAACAGCATATTGGC                                                 >  SRR4302534.4278903/1‑94 (MQ=60)
                                              TCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGC                                                 <  SRR4302534.3240536/94‑1 (MQ=60)
                                                CACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGACCCCCGGCGCCGCGGTTTGTTttttcccttaaacaacatatttgtgc                                               >  SRR4302534.258185/1‑68 (MQ=54)
                                                 CCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCT                                              <  SRR4302534.3751677/94‑1 (MQ=60)
                                                 ACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCT                                              <  SRR4302534.2923029/94‑1 (MQ=60)
                                                  CCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTG                                             <  SRR4302534.3515026/94‑1 (MQ=60)
                                                    GGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAA                                           <  SRR4302534.751589/94‑1 (MQ=60)
                                                    GGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAA                                           <  SRR4302534.2084897/94‑1 (MQ=60)
                                                        ATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTG                                       <  SRR4302534.182565/94‑1 (MQ=60)
                                                          GAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAG                                     <  SRR4302534.4736690/94‑1 (MQ=60)
                                                             AACCCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCCGGCGCAGCGGTTTGTTTTTTCACTTAAAAAACATATTGGCGCTGGAGGGGAGGTG                                  >  SRR4302534.4736319/1‑94 (MQ=40)
                                                                CCAGTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCCGCGGTTTGATATTCCACTGAAACAGCATATTGGCGCTGAAGGTGCGGTGTGC                               >  SRR4302534.2150674/1‑94 (MQ=60)
                                                                 CAGGCCAACGGTTCCCCCCTGCGCCAGTCTCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTTAGTTGTGCG                              <  SRR4302534.1973748/94‑1 (MQ=60)
                                                                  AGTCCAACGGTACACCCCTGCGCCAGGCACCCCCCGCGCCGCGGTTTTTTTTTTCACTGAAACAGCATATTTGCGCTGGAGGGGAGGTGTGCGT                             >  SRR4302534.5972090/1‑94 (MQ=23)
                                                                   GTCCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTT                            <  SRR4302534.47587/94‑1 (MQ=60)
                                                                     CCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAG                          <  SRR4302534.3515458/94‑1 (MQ=60)
                                                                     CCAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAG                          <  SRR4302534.2318769/94‑1 (MQ=60)
                                                                      CAACGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGC                         <  SRR4302534.1611114/94‑1 (MQ=60)
                                                                       AACGGTACACCCCTGCGCCCGGCACCCCCGGCGCAGCGTTTTGTTATTCCACTGAAAaaccaaattggggctgaaggggggtgttgggttaggg                        >  SRR4302534.4972345/1‑57 (MQ=60)
                                                                         CGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTC                      <  SRR4302534.5786452/94‑1 (MQ=60)
                                                                         CGGTACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTC                      <  SRR4302534.5837212/94‑1 (MQ=60)
                                                                             ACACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTTGCGCTGAAGGGGAGTTCTGCGTTAGCGTCAGCG                  >  SRR4302534.2043733/1‑94 (MQ=60)
                                                                              CACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGA                 <  SRR4302534.1590116/94‑1 (MQ=60)
                                                                              CACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGA                 <  SRR4302534.2094774/94‑1 (MQ=60)
                                                                               ACCCCTGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGAT                >  SRR4302534.5282095/1‑94 (MQ=60)
                                                                                   CTGCGCCAGTTACCCCTGGCGCAGCGCTTTGTTATTCCACTGAAAGAGCATATTGGTGCTGAGGGTGAGTTGTGCGTTAGCGTCCGGGATAAAG            >  SRR4302534.4073272/1‑94 (MQ=60)
                                                                                    TGCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGT           >  SRR4302534.1132933/1‑94 (MQ=60)
                                                                                     GCGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTA          >  SRR4302534.5738961/1‑94 (MQ=60)
                                                                                      CGCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTAT         <  SRR4302534.4740844/94‑1 (MQ=60)
                                                                                       GCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCAATAAAGTATT        <  SRR4302534.6175534/94‑1 (MQ=60)
                                                                                       GCCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGAGAGTTGTGAGTTAGCGTCGGCGATAAAGTATT        >  SRR4302534.353876/1‑94 (MQ=60)
                                                                                        CCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTG       >  SRR4302534.2103556/1‑94 (MQ=60)
                                                                                        CCAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTG       <  SRR4302534.2617099/94‑1 (MQ=60)
                                                                                         CAGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGC      >  SRR4302534.4942580/1‑94 (MQ=60)
                                                                                          AGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCG     >  SRR4302534.6119495/1‑94 (MQ=60)
                                                                                          AGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCG     <  SRR4302534.1932171/94‑1 (MQ=60)
                                                                                          AGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCG     >  SRR4302534.2664510/1‑94 (MQ=60)
                                                                                          AGGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCG     <  SRR4302534.3065291/94‑1 (MQ=60)
                                                                                           GGCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGC    >  SRR4302534.259916/1‑94 (MQ=60)
                                                                                            GCACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCG   <  SRR4302534.524306/94‑1 (MQ=60)
                                                                                             CACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGG  <  SRR4302534.4370797/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGG  >  NC_000913/1704959‑1705145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑