Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,790	T→C	S404P (TCT→CCT)	b1605 →	putative arginine/ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,790	0	T	C	98.5%	684.4 / ‑2.5	197	S404P (TCT→CCT)	b1605	putative arginine/ornithine antiporter
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (95/98); total (97/100)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 202 total aligned reads displayed.

GGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGAT  >  NC_000913/1678700‑1678879

ggCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCccct                                                                                         <  2:1477446/93‑1 (MQ=255)

 gcgcGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTg                                                                                        <  1:3049970/93‑1 (MQ=255)

  cgcgTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTgg                                                                                       >  2:4826172/1‑93 (MQ=255)

   gcgTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGt                                                                                      <  2:5399183/93‑1 (MQ=255)

   gcgTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGt                                                                                      <  2:4227978/93‑1 (MQ=255)

    cgTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTc                                                                                     >  1:4396891/1‑93 (MQ=255)

    cgTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTc                                                                                     <  2:3081978/93‑1 (MQ=255)

      ttCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCa                                                                                   >  2:2466371/1‑93 (MQ=255)

      ttCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCa                                                                                   >  1:5506774/1‑93 (MQ=255)

      ttCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCa                                                                                   >  2:5788948/1‑93 (MQ=255)

      ttCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCa                                                                                   >  1:2475492/1‑93 (MQ=255)

      ttCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCa                                                                                   >  1:2814927/1‑93 (MQ=255)

       tCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCaa                                                                                  >  2:2280564/1‑93 (MQ=255)

       tCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCaa                                                                                  >  1:4989353/1‑93 (MQ=255)

       tCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCaa                                                                                  <  1:4582045/93‑1 (MQ=255)

       tCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCaa                                                                                  <  2:2775352/93‑1 (MQ=255)

       tCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCaa                                                                                  <  1:1150241/93‑1 (MQ=255)

        cTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAAt                                                                                 <  2:4882517/93‑1 (MQ=255)

        cTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAAt                                                                                 >  2:3037259/1‑93 (MQ=255)

         ttGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATg                                                                                >  1:1709371/1‑93 (MQ=255)

          tGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGCCCAATGc                                                                               >  2:4417082/1‑93 (MQ=255)

           gCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGcc                                                                              >  2:2378095/1‑92 (MQ=255)

                aaaTCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGc                                                                         <  1:2070058/93‑1 (MQ=255)

                 aaTCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCt                                                                        <  2:767753/93‑1 (MQ=255)

                 aaTCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCt                                                                        <  1:5602386/93‑1 (MQ=255)

                   tCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGt                                                                      <  1:2555179/93‑1 (MQ=255)

                     gCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTa                                                                    <  1:3423197/93‑1 (MQ=255)

                     gCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTGATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTa                                                                    <  2:2699606/93‑1 (MQ=255)

                       aaCCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATc                                                                  <  2:3679263/93‑1 (MQ=255)

                        aCCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATcc                                                                 >  2:2200290/1‑93 (MQ=255)

                         cccGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCg                                                                >  1:5221324/1‑93 (MQ=255)

                         cccGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCg                                                                >  1:4050689/1‑93 (MQ=255)

                          ccGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgt                                                               >  1:2634876/1‑93 (MQ=255)

                          ccGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgt                                                               <  2:3587230/93‑1 (MQ=255)

                           cGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgtt                                                              <  1:4411950/93‑1 (MQ=255)

                           cGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgtt                                                              <  2:2952664/93‑1 (MQ=255)

                           cGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgtt                                                              >  1:3788966/1‑93 (MQ=255)

                            gTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgttg                                                             <  1:5592453/93‑1 (MQ=255)

                             tCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgttgt                                                            <  2:1304226/93‑1 (MQ=255)

                             tCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgttgt                                                            >  2:1925335/1‑93 (MQ=255)

                              ccACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCgttgtt                                                           >  2:5169986/1‑93 (MQ=255)

                               cACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTc                                                          <  1:1262506/93‑1 (MQ=255)

                               cACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTc                                                          <  1:952985/93‑1 (MQ=255)

                                aCTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCt                                                         >  1:490711/1‑93 (MQ=255)

                                aCTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCt                                                         >  1:5778678/1‑93 (MQ=255)

                                 cTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTg                                                        <  1:1590748/93‑1 (MQ=255)

                                   gCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTa                                                      <  1:2124494/93‑1 (MQ=255)

                                    cATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTAt                                                     <  1:4665296/93‑1 (MQ=255)

                                     aTAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATg                                                    >  2:2881966/1‑93 (MQ=255)

                                      tAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGc                                                   <  2:478058/93‑1 (MQ=255)

                                       aaaGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCa                                                  <  1:2585547/93‑1 (MQ=255)

                                            ggTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCggg                                             <  1:5678231/93‑1 (MQ=255)

                                              tAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGgc                                           <  2:4696922/93‑1 (MQ=255)

                                               aGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGgct                                          <  1:2380949/93‑1 (MQ=255)

                                               aGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGGTTTCCGTTGTTCTGTATGCACCGGGgct                                          >  1:5073403/1‑93 (MQ=255)

                                               aGGTGTCGGTGCCTGCATTTATGGCTTATGGGTATTTGACGCCCCTGGTCCAATGCACTTGCTGTTATTCGTTGTTCTGTATGCACCGGGgct                                          >  1:5299565/1‑93 (MQ=255)

                                                 gtgtCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGgctgc                                        <  1:3614911/93‑1 (MQ=255)

                                                  tgtCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGgctgct                                       <  2:2626110/93‑1 (MQ=255)

                                                    tCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTgg                                     >  1:4110300/1‑93 (MQ=255)

                                                    tCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCACTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTgg                                     <  2:4544585/93‑1 (MQ=255)

                                                      ggTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGtt                                   <  2:4072274/93‑1 (MQ=255)

                                                      ggTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGtt                                   >  1:2719706/1‑93 (MQ=255)

                                                       gTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGttt                                  >  2:613083/1‑93 (MQ=255)

                                                        tGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGtttt                                 >  2:1084523/1‑93 (MQ=255)

                                                         gCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTc                                >  2:1225076/1‑93 (MQ=255)

                                                         gCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTc                                >  2:179283/1‑93 (MQ=255)

                                                          ccTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTcc                               <  1:1941542/93‑1 (MQ=255)

                                                          ccTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTcc                               >  2:3336794/1‑93 (MQ=255)

                                                          ccTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGCTTTcc                               >  2:4206555/1‑93 (MQ=255)

                                                           cTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCt                              <  1:3907139/93‑1 (MQ=255)

                                                           cTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCt                              <  1:3852804/93‑1 (MQ=255)

                                                           cTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCt                              <  1:587498/93‑1 (MQ=255)

                                                             gCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGt                            >  2:4388397/1‑93 (MQ=255)

                                                             gCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGt                            <  2:1636638/93‑1 (MQ=255)

                                                              cATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTa                           <  2:6039850/93‑1 (MQ=255)

                                                              cATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTa                           <  1:967408/93‑1 (MQ=255)

                                                                tttATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTAcg                         <  1:1875861/93‑1 (MQ=255)

                                                                    tGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTAcgcgcg                     >  2:2240415/1‑93 (MQ=255)

                                                                     ggCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACgcgcgc                    >  1:3526620/1‑93 (MQ=255)

                                                                     ggCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACgcgcgc                    <  2:593140/93‑1 (MQ=255)

                                                                      gCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCa                   >  1:1090753/1‑93 (MQ=255)

                                                                        ttATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCaaa                 >  2:1617983/1‑93 (MQ=255)

                                                                         tATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCaaaa                >  2:3661535/1‑93 (MQ=255)

                                                                          aTGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAAc               <  1:772265/93‑1 (MQ=255)

                                                                           tGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACg              >  1:6043109/1‑93 (MQ=255)

                                                                           tGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACg              <  1:2406832/93‑1 (MQ=255)

                                                                             gTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGGAAAACGCa            >  1:5619237/1‑93 (MQ=255)

                                                                             gTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCa            >  1:3881466/1‑93 (MQ=255)

                                                                             gTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCa            >  1:2193477/1‑93 (MQ=255)

                                                                             gTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCa            >  2:834825/1‑93 (MQ=255)

                                                                              ttATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCAt           <  1:59516/93‑1 (MQ=255)

                                                                              ttATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCAt           <  2:5064475/93‑1 (MQ=255)

                                                                               tATTGTACGCCCCTGGTCCAGTGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATa          <  1:1008824/93‑1 (MQ=255)

                                                                               tATTGTACGCCCCTGGTCCAATGCACTTGTTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATa          >  2:728351/1‑93 (MQ=255)

                                                                               tATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATa          <  2:3704799/93‑1 (MQ=255)

                                                                               tATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATa          >  1:5378521/1‑93 (MQ=255)

                                                                                 ttGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATAcc        <  1:5053672/93‑1 (MQ=255)

                                                                                  tGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATAccc       >  2:4705828/1‑93 (MQ=255)

                                                                                    tACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCAt     >  1:484103/1‑93 (MQ=255)

                                                                                      cGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGa   >  1:3428479/1‑93 (MQ=255)

GGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGAT  >  NC_000913/1678700‑1678879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATAATG  >  NC_000913/1678697‑1678883

GTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCC                                                                                               <  SRR4302532.685664/94‑1 (MQ=60)

  CGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCT                                                                                             >  SRR4302532.3983608/1‑94 (MQ=60)

    GCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGG                                                                                           <  SRR4302532.3150651/94‑1 (MQ=60)

      GCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTC                                                                                         >  SRR4302532.4546499/1‑94 (MQ=60)

        GTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCA                                                                                       >  SRR4302532.2557938/1‑94 (MQ=60)

        GTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCA                                                                                       >  SRR4302532.5697795/1‑94 (MQ=60)

        GTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCA                                                                                       >  SRR4302532.2909149/1‑94 (MQ=60)

         TTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAA                                                                                      >  SRR4302532.3977026/1‑94 (MQ=60)

         TTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAA                                                                                      >  SRR4302532.5160366/1‑94 (MQ=60)

         TTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGCTATTGTACGCCCCTGGTCCAA                                                                                      >  SRR4302532.6249747/1‑94 (MQ=60)

          TCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAAT                                                                                     <  SRR4302532.1188636/94‑1 (MQ=60)

          TCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAAT                                                                                     <  SRR4302532.4738275/94‑1 (MQ=60)

           CTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATG                                                                                    >  SRR4302532.1766996/1‑94 (MQ=60)

               CTGAAAGTCACAACCCGTCCACTGCATAATGCGGTAGTCGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACT                                                                                <  SRR4302532.2746637/94‑1 (MQ=60)

               CTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACT                                                                                <  SRR4302532.403919/94‑1 (MQ=60)

                   AAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCT                                                                            <  SRR4302532.2139045/94‑1 (MQ=60)

                    AATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTG                                                                           <  SRR4302532.5796855/94‑1 (MQ=60)

                      TCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTT                                                                         <  SRR4302532.2680039/94‑1 (MQ=60)

                      TCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTT                                                                         <  SRR4302532.2640406/94‑1 (MQ=60)

                       CGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTA                                                                        <  SRR4302532.2149244/94‑1 (MQ=60)

                        GCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTAT                                                                       <  SRR4302532.3537869/94‑1 (MQ=60)

                         CAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATC                                                                      >  SRR4302532.4757625/1‑94 (MQ=60)

                          AACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCC                                                                     >  SRR4302532.2234005/1‑94 (MQ=60)

                          AACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAAAGCACTTGCTGTTATCC                                                                     <  SRR4302532.5824392/94‑1 (MQ=60)

                           ACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCG                                                                    >  SRR4302532.4186996/1‑94 (MQ=60)

                           ACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCG                                                                    >  SRR4302532.5401383/1‑94 (MQ=60)

                            CCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGT                                                                   >  SRR4302532.5794849/1‑94 (MQ=60)

                            CCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGT                                                                   >  SRR4302532.2723159/1‑94 (MQ=60)

                             CCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTT                                                                  <  SRR4302532.5483977/94‑1 (MQ=60)

                             CCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTT                                                                  >  SRR4302532.3916789/1‑94 (MQ=60)

                             CCGTCCACTGCATAAAGCGGTAGATGTCGGTTCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCTCTTGCTGTTATCCGTT                                                                  <  SRR4302532.3103390/94‑1 (MQ=60)

                              CGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTG                                                                 >  SRR4302532.5258235/1‑94 (MQ=60)

                              CGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTG                                                                 <  SRR4302532.4562246/94‑1 (MQ=60)

                              CGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTG                                                                 >  SRR4302532.5813606/1‑94 (MQ=60)

                              CGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTG                                                                 <  SRR4302532.1591159/94‑1 (MQ=60)

                               GTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGT                                                                <  SRR4302532.5786581/94‑1 (MQ=60)

                                TCCACTGTATAAAGCGGTAGGTGTCGGCGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTT                                                               <  SRR4302532.1100464/94‑1 (MQ=60)

                                  CACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGTTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCT                                                             <  SRR4302532.5923857/94‑1 (MQ=60)

                                  CACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCT                                                             >  SRR4302532.507864/1‑94 (MQ=60)

                                  CACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCT                                                             <  SRR4302532.985547/94‑1 (MQ=60)

                                  CACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCT                                                             >  SRR4302532.5979698/1‑94 (MQ=60)

                                  CACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCT                                                             <  SRR4302532.1304736/94‑1 (MQ=60)

                                   ACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTG                                                            >  SRR4302532.2137000/1‑94 (MQ=60)

                                    CTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGT                                                           <  SRR4302532.1644596/94‑1 (MQ=60)

                                     TGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTA                                                          <  SRR4302532.165962/94‑1 (MQ=60)

                                      GCATAAAGCGGTAGGTGTCTGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTAT                                                         <  SRR4302532.4248118/94‑1 (MQ=60)

                                      GCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTAT                                                         <  SRR4302532.2195469/94‑1 (MQ=60)

                                       CATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATG                                                        <  SRR4302532.4824354/94‑1 (MQ=60)

                                        ATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGC                                                       <  SRR4302532.98979/94‑1 (MQ=60)

                                         TAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCA                                                      <  SRR4302532.5197960/94‑1 (MQ=60)

                                          AAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCAC                                                     <  SRR4302532.2671970/94‑1 (MQ=60)

                                           AAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACC                                                    <  SRR4302532.3650735/94‑1 (MQ=60)

                                            AGCGGTAGGTGTCGGTTCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCG                                                   <  SRR4302532.4546823/94‑1 (MQ=60)

                                            AGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCG                                                   <  SRR4302532.3538937/94‑1 (MQ=60)

                                             GCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGG                                                  <  SRR4302532.2569546/94‑1 (MQ=60)

                                              CGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGG                                                 <  SRR4302532.692926/94‑1 (MQ=60)

                                               GGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGG                                                <  SRR4302532.1187411/94‑1 (MQ=60)

                                               GGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGG                                                <  SRR4302532.5876477/94‑1 (MQ=60)

                                               GGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGG                                                >  SRR4302532.5235192/1‑94 (MQ=60)

                                               GGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGG                                                <  SRR4302532.5014126/94‑1 (MQ=60)

                                               GGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGC                                                >  SRR4302532.5455584/1‑94 (MQ=60)

                                                GTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATACGTTGTTCTGTATGCACCGGGGC                                               >  SRR4302532.3598605/1‑94 (MQ=60)

                                                 TAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCT                                              <  SRR4302532.2326973/94‑1 (MQ=60)

                                                 TAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGGTTTCCGTTGTTCTGTATGCACCGGGGCT                                              >  SRR4302532.5247795/1‑94 (MQ=60)

                                                 TAGGTGTCGGTGCCTGCATTTATGGCTTATGGGTATTTGACGCCCCTGGTCCAATGCACTTGCTGTTATTCGTTGTTCTGTATGCACCGGGGCT                                              >  SRR4302532.5482661/1‑94 (MQ=60)

                                                 TAGATGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCT                                              <  SRR4302532.2870777/94‑1 (MQ=60)

                                                  AGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTG                                             <  SRR4302532.2460280/94‑1 (MQ=60)

                                                   GGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGC                                            >  SRR4302532.2655626/1‑94 (MQ=60)

                                                    GTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCT                                           <  SRR4302532.3735811/94‑1 (MQ=60)

                                                      GTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGG                                         >  SRR4302532.4248274/1‑94 (MQ=60)

                                                      GTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGG                                         >  SRR4302532.5602052/1‑94 (MQ=60)

                                                        CGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTT                                       >  SRR4302532.2811179/1‑94 (MQ=60)

                                                          GTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTT                                     >  SRR4302532.3848524/1‑94 (MQ=60)

                                                          GTGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTT                                     >  SRR4302532.6152557/1‑94 (MQ=60)

                                                           TGCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTC                                    >  SRR4302532.5134729/1‑94 (MQ=60)

                                                            GCCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCC                                   <  SRR4302532.4648730/94‑1 (MQ=60)

                                                             CCTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCT                                  <  SRR4302532.2006097/94‑1 (MQ=60)

                                                              CTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTG                                 <  SRR4302532.4038888/94‑1 (MQ=60)

                                                              CTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTG                                 <  SRR4302532.607998/94‑1 (MQ=60)

                                                              CTGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTG                                 <  SRR4302532.3982880/94‑1 (MQ=60)

                                                               TGCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGT                                <  SRR4302532.4165803/94‑1 (MQ=60)

                                                                GCATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTA                               <  SRR4302532.2480639/94‑1 (MQ=60)

                                                                 CATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTAC                              <  SRR4302532.1000422/94‑1 (MQ=60)

                                                                 CATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTAC                              <  SRR4302532.3268045/94‑1 (MQ=60)

                                                                  ATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACG                             <  SRR4302532.59922/94‑1 (MQ=60)

                                                                  ATTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACG                             <  SRR4302532.2552105/94‑1 (MQ=60)

                                                                   TTTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGC                            <  SRR4302532.1937996/94‑1 (MQ=60)

                                                                    TTATGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCG                           >  SRR4302532.1983462/1‑94 (MQ=60)

                                                                       TGGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGC                        >  SRR4302532.3644679/1‑94 (MQ=60)

                                                                        GGCTTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCA                       >  SRR4302532.1127248/1‑94 (MQ=60)

                                                                         cgTTATGGTTATATTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTATATGCACGGGGGCTGCTGGTTTTCCTGTACGCGCGAAA                      <  SRR4302532.2478990/92‑1 (MQ=60)

                                                                           TTATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAA                    >  SRR4302532.1260245/1‑94 (MQ=60)

                                                                            TATGGTTATTGTACGCCCGTGGTACAATGCACTTGCTGTTATCCGTTGTTCTGTATGCGCCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAAC                   <  SRR4302532.5713393/94‑1 (MQ=60)

                                                                             ATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACG                  >  SRR4302532.6251772/1‑94 (MQ=60)

                                                                             ATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACG                  <  SRR4302532.798910/94‑1 (MQ=60)

                                                                             ATGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACG                  <  SRR4302532.1888725/94‑1 (MQ=60)

                                                                              TGGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGC                 <  SRR4302532.2486920/94‑1 (MQ=60)

                                                                               GGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGGAAAACGCA                >  SRR4302532.5814212/1‑94 (MQ=60)

                                                                               GGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCA                >  SRR4302532.2266556/1‑94 (MQ=60)

                                                                               GGTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCA                >  SRR4302532.4012480/1‑94 (MQ=60)

                                                                                GTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCAT               <  SRR4302532.2777064/94‑1 (MQ=60)

                                                                                GTTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCAT               >  SRR4302532.6208547/1‑94 (MQ=60)

                                                                                 TTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATA              >  SRR4302532.5564829/1‑94 (MQ=60)

                                                                                 TTATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATA              <  SRR4302532.61663/94‑1 (MQ=60)

                                                                                  TATTGTACGCCCCTGGTCCAGTGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATAC             <  SRR4302532.1043135/94‑1 (MQ=60)

                                                                                   ATTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACC            <  SRR4302532.4997199/94‑1 (MQ=60)

                                                                                    TTGTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCC           <  SRR4302532.5227120/94‑1 (MQ=60)

                                                                                      GTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCAT         >  SRR4302532.501007/1‑94 (MQ=60)

                                                                                      GTACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCAT         <  SRR4302532.860392/94‑1 (MQ=60)

                                                                                        ACGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGA       >  SRR4302532.3543344/1‑94 (MQ=60)

                                                                                         CGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGAT      >  SRR4302532.4429589/1‑94 (MQ=60)

                                                                                         CGCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACACATGAT      >  SRR4302532.1009560/1‑94 (MQ=60)

                                                                                          GCCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATA     >  SRR4302532.3273817/1‑94 (MQ=60)

                                                                                           CCCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATAA    <  SRR4302532.6055618/94‑1 (MQ=60)

                                                                                            CCCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATAAT   <  SRR4302532.3124729/94‑1 (MQ=60)

                                                                                             CCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATAATG  >  SRR4302532.5614606/1‑94 (MQ=60)

                                                                                             CCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATAATG  <  SRR4302532.2275440/94‑1 (MQ=60)

GTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGTTATCCGTTGTTCTGTATGCACCGGGGCTGCTGGTTTTCCTGTACGCGCGCAAAACGCATACCCATGATAATG  >  NC_000913/1678697‑1678883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`