Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,008,480 +A coding (637/654 nt) yedK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,008,4761.A98.0% 724.4 / 5.5 195coding (633/654 nt)yedKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base A (84/107);  total (87/108)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 213 total aligned reads displayed.

AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563
                                                                                              |                                                                                         
 gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑a                                                                                          >  1:1284935/1‑93 (MQ=255)
 gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGGAAT‑GTT‑a                                                                                          >  2:229372/1‑93 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa‑aaa                                                                                     <  1:5307996/93‑2 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa‑aaa                                                                                     >  1:4331428/1‑92 (MQ=255)
        agaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa‑aaacca                                                                                  >  2:438505/1‑89 (MQ=255)
        agaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa‑aaacca                                                                                  <  2:2446036/93‑5 (MQ=255)
        agaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa‑aaacca                                                                                  <  1:5686371/93‑5 (MQ=255)
        agaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa‑aaacca                                                                                  <  1:4975522/93‑5 (MQ=255)
          aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAgg                                                                                <  2:5406616/93‑1 (MQ=255)
          aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAgg                                                                                <  2:2565506/93‑1 (MQ=255)
          aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAgg                                                                                <  1:3117072/93‑1 (MQ=255)
           agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAggg                                                                               <  2:3981935/93‑1 (MQ=255)
            gCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGgc                                                                              <  2:426743/93‑1 (MQ=255)
            gCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGgc                                                                              <  1:576461/93‑1 (MQ=255)
             ccTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGgcg                                                                             >  2:4565065/1‑93 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGTACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGgcgc                                                                            >  1:2661338/1‑93 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGgcgc                                                                            >  1:1367704/1‑93 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGcgcg                                                                           <  2:5729393/93‑1 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGcgcg                                                                           <  1:3354844/93‑1 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGcgcg                                                                           <  1:775044/93‑1 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGGATCGCGCGCGGAGGGTAAT‑GTTAAA‑AA‑CCAGGGcgcg                                                                           >  2:2267155/1‑93 (MQ=255)
                cAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCgg                                                                          <  2:2737355/93‑1 (MQ=255)
                 aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGa                                                                         <  1:354522/93‑1 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAg                                                                        >  1:2336889/1‑93 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAg                                                                        <  1:5190405/93‑1 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAg                                                                        >  1:2182577/1‑93 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAg                                                                        >  1:916445/1‑93 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGt                                                                       <  1:4324790/93‑1 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGt                                                                       <  1:4714136/93‑1 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGt                                                                       <  2:3095323/93‑1 (MQ=255)
                     aTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTa                                                                     <  1:4948776/93‑1 (MQ=255)
                      tCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTat                                                                    >  2:1265296/1‑92 (MQ=255)
                         cgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTc                                                                 <  2:4322003/93‑1 (MQ=255)
                          ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCa                                                                >  2:1701318/1‑93 (MQ=255)
                            ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAAc                                                              <  2:4528567/93‑1 (MQ=255)
                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCt                                                            <  1:5816579/93‑1 (MQ=255)
                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCt                                                            >  1:3514467/1‑93 (MQ=255)
                               gTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTg                                                           >  2:631466/1‑93 (MQ=255)
                                tGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGt                                                          >  1:338373/1‑93 (MQ=255)
                                 ggCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGtt                                                         <  2:5624992/93‑1 (MQ=255)
                                  gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGttt                                                        >  2:739548/1‑93 (MQ=255)
                                   cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTg                                                       <  1:5425025/93‑1 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      >  1:4669113/1‑93 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      >  1:163128/1‑93 (MQ=255)
                                     gtgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                                     <  1:3282680/93‑1 (MQ=255)
                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    <  1:2785176/93‑1 (MQ=255)
                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑AACAA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    >  1:2367764/1‑93 (MQ=255)
                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  2:5657367/93‑1 (MQ=255)
                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  2:4959278/93‑1 (MQ=255)
                                        ttCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGGTA‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGCTTGATAAg                                                  >  2:5475687/1‑93 (MQ=255)
                                        ttCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAg                                                  <  1:424355/93‑1 (MQ=255)
                                        ttCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAg                                                  >  1:1867138/1‑93 (MQ=255)
                                         tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGc                                                 <  1:4265770/93‑1 (MQ=255)
                                         tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGc                                                 <  2:873883/93‑1 (MQ=255)
                                           cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                                               <  1:6034573/93‑1 (MQ=255)
                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              <  1:3187585/93‑1 (MQ=255)
                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCACGGCGCGGAGTTAATTCAACCTGTTTGATAAGccgg                                              >  2:3286243/1‑91 (MQ=255)
                                              cAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGga                                            >  2:3760031/1‑93 (MQ=255)
                                                aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgaga                                          <  1:4509370/93‑1 (MQ=255)
                                                aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgaga                                          >  1:5254414/1‑93 (MQ=255)
                                                 aCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                         >  1:5232607/1‑93 (MQ=255)
                                                  ccAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatt                                        >  2:3512115/1‑93 (MQ=255)
                                                   cAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatta                                       >  2:2017442/1‑93 (MQ=255)
                                                   cAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatta                                       <  2:5595124/93‑1 (MQ=255)
                                                    aGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattat                                      >  1:191442/1‑93 (MQ=255)
                                                     gTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattatt                                     <  2:5405703/93‑1 (MQ=255)
                                                     gTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattatt                                     <  1:5931110/93‑1 (MQ=255)
                                                      ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTg                                    <  2:1583640/93‑1 (MQ=255)
                                                      ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTg                                    <  1:2252721/93‑1 (MQ=255)
                                                      ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTg                                    <  1:1004666/93‑1 (MQ=255)
                                                       tctcCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGa                                   <  2:772051/93‑1 (MQ=255)
                                                            tGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaaa                              >  2:4728618/1‑92 (MQ=255)
                                                             ggCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaaca                             <  2:616052/93‑1 (MQ=255)
                                                             ggCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaaca                             <  2:2071264/93‑1 (MQ=255)
                                                              gCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaacaa                            <  1:1593464/93‑1 (MQ=255)
                                                              gCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaacaa                            <  2:767047/93‑1 (MQ=255)
                                                                aCCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          >  1:1436419/1‑93 (MQ=255)
                                                                aCCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          <  2:5275430/93‑1 (MQ=255)
                                                                aCCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          <  1:3738304/93‑1 (MQ=255)
                                                                 cccGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                         <  2:4141307/93‑1 (MQ=255)
                                                                  ccGGTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                        <  1:6109751/93‑1 (MQ=255)
                                                                     gTATCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                     >  1:5597073/1‑93 (MQ=255)
                                                                       aTCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc                   >  1:213318/1‑93 (MQ=255)
                                                                       aTCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc                   <  2:2258688/93‑1 (MQ=255)
                                                                        tCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                  >  1:5148107/1‑93 (MQ=255)
                                                                        tCGCGCGCGGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                  >  1:5975445/1‑93 (MQ=255)
                                                                         cgcgcgcgGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCAt                 <  1:997840/93‑1 (MQ=255)
                                                                          gcgcgcgGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATa                >  2:3696126/1‑93 (MQ=255)
                                                                               cgGTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  1:4428961/93‑1 (MQ=255)
                                                                                ggTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAt          >  2:4774999/1‑93 (MQ=255)
                                                                                 gTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         <  2:3235101/93‑1 (MQ=255)
                                                                                 gTGGGTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         <  2:951245/93‑1 (MQ=255)
                                                                                   gggTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       <  1:4137184/93‑1 (MQ=255)
                                                                                   gggTAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       >  1:3627455/1‑93 (MQ=255)
                                                                                   gggTAAT‑GTT‑AA‑AAGCCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       <  2:5903911/93‑1 (MQ=255)
                                                                                      tAAT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAt    >  2:3933158/1‑93 (MQ=255)
                                                                                       aaT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAtt   <  1:3801534/93‑1 (MQ=255)
                                                                                        aT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  >  2:3433348/1‑93 (MQ=255)
                                                                                        aT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  >  2:3856118/1‑93 (MQ=255)
                                                                                        aT‑GTTAAA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  >  1:2575964/1‑93 (MQ=255)
                                                                                              |                                                                                         
AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑AA‑AA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  NC_000913/2008388‑2008573
                                                                                              |                                                                                             
GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AA‑AAA                                                                                              >  SRR4302532.4477759/1‑94 (MQ=60)
 TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AA‑AAAC                                                                                             <  SRR4302532.5491520/94‑1 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAG                                                                                           <  SRR4302532.5884848/94‑1 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAG                                                                                           >  SRR4302532.3333057/1‑94 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAG                                                                                           >  SRR4302532.3070170/1‑94 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAG                                                                                           >  SRR4302532.5992699/1‑94 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAG                                                                                           <  SRR4302532.5146078/94‑1 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAG                                                                                           >  SRR4302532.1498180/1‑94 (MQ=60)
     GAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGG                                                                                          <  SRR4302532.4125358/94‑1 (MQ=60)
     GAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGAGCGGTGGGTAATGTTAAA‑AACCAGG                                                                                          <  SRR4302532.6117952/94‑1 (MQ=60)
      AAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGG                                                                                         <  SRR4302532.3220487/94‑1 (MQ=60)
       AGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGC                                                                                        >  SRR4302532.4474661/1‑94 (MQ=60)
       AGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGC                                                                                        <  SRR4302532.5136239/94‑1 (MQ=60)
        GCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCG                                                                                       <  SRR4302532.291534/94‑1 (MQ=60)
        GCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCG                                                                                       <  SRR4302532.596560/94‑1 (MQ=60)
        GCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCG                                                                                       <  SRR4302532.3333482/94‑1 (MQ=60)
         CCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGTACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGC                                                                                      >  SRR4302532.2750659/1‑94 (MQ=60)
         CCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGC                                                                                      >  SRR4302532.1413513/1‑94 (MQ=60)
           TCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGG                                                                                    <  SRR4302532.3466855/94‑1 (MQ=60)
           TCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGG                                                                                    <  SRR4302532.801740/94‑1 (MQ=60)
            CAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGA                                                                                   >  SRR4302532.2614344/1‑94 (MQ=60)
             AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAG                                                                                  <  SRR4302532.366795/94‑1 (MQ=60)
             AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAG                                                                                  >  SRR4302532.2255405/1‑94 (MQ=60)
             AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAG                                                                                  >  SRR4302532.947794/1‑94 (MQ=60)
             AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAG                                                                                  >  SRR4302532.2414740/1‑94 (MQ=60)
              GAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGT                                                                                 <  SRR4302532.5369287/94‑1 (MQ=60)
              GAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGT                                                                                 >  SRR4302532.2439000/1‑94 (MQ=60)
               AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTT                                                                                >  SRR4302532.3226443/1‑94 (MQ=60)
               AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTT                                                                                <  SRR4302532.4470927/94‑1 (MQ=60)
               AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTT                                                                                <  SRR4302532.4874983/94‑1 (MQ=60)
                AATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTA                                                                               >  SRR4302532.3043888/1‑94 (MQ=60)
                 ATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAA                                                                              <  SRR4302532.5399172/94‑1 (MQ=60)
                 ATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAA                                                                              <  SRR4302532.5118606/94‑1 (MQ=60)
                   CGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATT                                                                            >  SRR4302532.2778815/1‑94 (MQ=60)
                    GCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTC                                                                           <  SRR4302532.3855796/94‑1 (MQ=60)
                    GCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTC                                                                           <  SRR4302532.343428/94‑1 (MQ=60)
                      GGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAA                                                                         >  SRR4302532.5975759/1‑94 (MQ=60)
                      GGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAA                                                                         <  SRR4302532.1985005/94‑1 (MQ=60)
                         CAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCT                                                                      <  SRR4302532.2724179/94‑1 (MQ=60)
                         CAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCT                                                                      >  SRR4302532.5407347/1‑94 (MQ=60)
                         CAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCT                                                                      >  SRR4302532.3632136/1‑94 (MQ=60)
                          AGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTG                                                                     <  SRR4302532.6018615/94‑1 (MQ=60)
                          AGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTG                                                                     <  SRR4302532.4960165/94‑1 (MQ=60)
                           GTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGT                                                                    >  SRR4302532.350106/1‑94 (MQ=60)
                           GTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGT                                                                    >  SRR4302532.350104/1‑94 (MQ=60)
                               CTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGA                                                                >  SRR4302532.4828307/1‑94 (MQ=60)
                               CTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGA                                                                <  SRR4302532.5613054/94‑1 (MQ=60)
                               CTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGA                                                                >  SRR4302532.168574/1‑94 (MQ=60)
                                TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGTT                                                               >  SRR4302532.533725/1‑94 (MQ=60)
                                TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGAT                                                               <  SRR4302532.4551680/94‑1 (MQ=60)
                                TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGAT                                                               >  SRR4302532.1685110/1‑94 (MQ=60)
                                 GTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATA                                                              <  SRR4302532.3391884/94‑1 (MQ=60)
                                 GTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AACAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATA                                                              >  SRR4302532.2446730/1‑94 (MQ=60)
                                  TGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAA                                                             >  SRR4302532.6110553/1‑94 (MQ=60)
                                  TGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAA                                                             <  SRR4302532.2878602/94‑1 (MQ=60)
                                  TGTTCCAGCAAACCAGTGCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAA                                                             <  SRR4302532.230738/94‑1 (MQ=60)
                                   GTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAG                                                            >  SRR4302532.1464326/1‑94 (MQ=60)
                                   GTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAG                                                            >  SRR4302532.1928992/1‑94 (MQ=60)
                                    TTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGC                                                           <  SRR4302532.439056/94‑1 (MQ=60)
                                     TCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCC                                                          <  SRR4302532.4409357/94‑1 (MQ=60)
                                      CCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCT                                                         <  SRR4302532.6155551/94‑1 (MQ=60)
                                       CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        <  SRR4302532.6242989/94‑1 (MQ=60)
                                       CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        <  SRR4302532.6242988/94‑1 (MQ=60)
                                        AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       <  SRR4302532.962486/94‑1 (MQ=60)
                                        AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       <  SRR4302532.5736928/94‑1 (MQ=60)
                                        AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       <  SRR4302532.3293415/94‑1 (MQ=60)
                                           AAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGA                                                    >  SRR4302532.5435565/1‑94 (MQ=60)
                                            AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                   >  SRR4302532.5413044/1‑94 (MQ=60)
                                            AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                   <  SRR4302532.1300920/94‑1 (MQ=60)
                                            AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                   <  SRR4302532.4663334/94‑1 (MQ=60)
                                              CCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTA                                                 >  SRR4302532.4598961/1‑94 (MQ=60)
                                               CAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTAT                                                <  SRR4302532.441703/94‑1 (MQ=60)
                                               CAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTAT                                                <  SRR4302532.1603196/94‑1 (MQ=60)
                                               CAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTAT                                                >  SRR4302532.197887/1‑94 (MQ=60)
                                                AGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATT                                               >  SRR4302532.428460/1‑94 (MQ=60)
                                                AGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATT                                               <  SRR4302532.456443/94‑1 (MQ=60)
                                                 GTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTG                                              >  SRR4302532.5425944/1‑94 (MQ=60)
                                                 GTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTG                                              <  SRR4302532.6136424/94‑1 (MQ=60)
                                                 GTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTG                                              >  SRR4302532.5850852/1‑94 (MQ=60)
                                                  TTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGA                                             <  SRR4302532.1038855/94‑1 (MQ=60)
                                                  TTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGA                                             <  SRR4302532.2327492/94‑1 (MQ=60)
                                                   TCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAT                                            <  SRR4302532.2159369/94‑1 (MQ=60)
                                                   TCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAT                                            <  SRR4302532.5574162/94‑1 (MQ=60)
                                                      CCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAA                                         >  SRR4302532.1399472/1‑94 (MQ=60)
                                                         GGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAA                                      >  SRR4302532.4863810/1‑94 (MQ=60)
                                                         GGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAA                                      <  SRR4302532.4142706/94‑1 (MQ=60)
                                                          GCACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAT                                     <  SRR4302532.1647405/94‑1 (MQ=60)
                                                           CACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATA                                    >  SRR4302532.1484803/1‑94 (MQ=60)
                                                            ACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   <  SRR4302532.3863912/94‑1 (MQ=60)
                                                            ACCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   >  SRR4302532.3483063/1‑94 (MQ=60)
                                                             CCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACT                                  <  SRR4302532.817113/94‑1 (MQ=60)
                                                             CCCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGACTGGAGATTATTGATCAACAATACT                                  >  SRR4302532.689715/1‑94 (MQ=60)
                                                              CCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTG                                 >  SRR4302532.3903249/1‑94 (MQ=60)
                                                              CCGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTG                                 <  SRR4302532.6320640/94‑1 (MQ=60)
                                                               CGGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGC                                >  SRR4302532.546087/1‑94 (MQ=60)
                                                               CGGTATCGAGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGC                                >  SRR4302532.6240746/1‑94 (MQ=60)
                                                                GGTATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               >  SRR4302532.5791372/1‑94 (MQ=60)
                                                                  TATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTC                             >  SRR4302532.220576/1‑94 (MQ=60)
                                                                   ATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302532.6181987/1‑94 (MQ=60)
                                                                   ATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302532.5325208/1‑94 (MQ=60)
                                                                   ATCGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302532.2221340/1‑94 (MQ=60)
                                                                     CGCGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATA                          <  SRR4302532.1031843/94‑1 (MQ=60)
                                                                       CGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                        <  SRR4302532.5893573/94‑1 (MQ=60)
                                                                       CGCGCGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                        <  SRR4302532.5217670/94‑1 (MQ=60)
                                                                       CGCGCAGTGGGTAAGGTTAAA‑AACCAGGGCGCGGAGTTAATTCATCCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                        <  SRR4302532.441173/94‑1 (MQ=60)
                                                                           CGGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302532.4579846/94‑1 (MQ=60)
                                                                            GGTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATC                   >  SRR4302532.3779455/1‑94 (MQ=60)
                                                                             GTGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCT                  <  SRR4302532.235379/94‑1 (MQ=60)
                                                                             GTGGGTAATGTTAAA‑AACCAGGGAGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCT                  <  SRR4302532.3656991/94‑1 (MQ=60)
                                                                              TGGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTC                 >  SRR4302532.3749090/1‑94 (MQ=60)
                                                                               GGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                >  SRR4302532.1937651/1‑94 (MQ=60)
                                                                               GGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                <  SRR4302532.1785650/94‑1 (MQ=60)
                                                                               GGGTAATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                <  SRR4302532.4275963/94‑1 (MQ=60)
                                                                                   AATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            >  SRR4302532.2662015/1‑94 (MQ=60)
                                                                                   AATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            <  SRR4302532.6079549/94‑1 (MQ=60)
                                                                                   AATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            <  SRR4302532.3929845/94‑1 (MQ=60)
                                                                                    ATGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG           >  SRR4302532.393105/1‑94 (MQ=60)
                                                                                     TGTTAAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGA          <  SRR4302532.646754/94‑1 (MQ=60)
                                                                                       ttaAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA        <  SRR4302532.2528535/91‑1 (MQ=60)
                                                                                       ttaAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA        >  SRR4302532.187348/4‑94 (MQ=60)
                                                                                       ttaAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA        >  SRR4302532.187349/4‑94 (MQ=60)
                                                                                       tgaAA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA        <  SRR4302532.4779190/91‑1 (MQ=60)
                                                                                           A‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGA    <  SRR4302532.68814/94‑1 (MQ=60)
                                                                                              ACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  SRR4302532.567244/1‑94 (MQ=60)
                                                                                              |                                                                                             
GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AA‑AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  NC_000913/2008388‑2008573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑