Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,313,133	T→C	P542P (CCT→CCC)	yojN →	putative 2‑component sensor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,313,133	0	T	C	99.5%	769.4 / ‑4.6	211	P542P (CCT→CCC)	yojN	putative 2‑component sensor protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (114/96); total (114/97)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 226 total aligned reads displayed.

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACG  >  NC_000913/2313043‑2313222

gAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTtgcccgc                                                                                         >  1:1208664/1‑93 (MQ=255)

 aaCATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTtgcccgcc                                                                                        <  1:4695874/93‑1 (MQ=255)

   cATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCAt                                                                                      <  2:5849140/93‑1 (MQ=255)

    aTGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATc                                                                                     >  1:1769151/1‑93 (MQ=255)

    aTGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATc                                                                                     <  1:1669219/93‑1 (MQ=255)

     tGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCa                                                                                    >  1:4043986/1‑93 (MQ=255)

     tGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCa                                                                                    >  1:5602572/1‑93 (MQ=255)

      gCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCaa                                                                                   >  2:4027191/1‑93 (MQ=255)

        ttGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGc                                                                                 >  1:1296206/1‑93 (MQ=255)

        ttGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGc                                                                                 >  1:880085/1‑93 (MQ=255)

         tGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCg                                                                                <  1:141775/93‑1 (MQ=255)

          gCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGt                                                                               <  1:1866627/93‑1 (MQ=255)

            ggATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaa                                                                             >  1:1897737/1‑93 (MQ=255)

            ggATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaa                                                                             >  2:5676573/1‑93 (MQ=255)

            ggATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaa                                                                             >  1:4835578/1‑93 (MQ=255)

            ggATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGGGTTGCCCGCCATCAAGCGTaa                                                                             <  2:2878374/93‑1 (MQ=255)

             gatgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaaa                                                                            >  2:1329119/1‑93 (MQ=255)

             gatgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaaa                                                                            <  2:3643554/93‑1 (MQ=255)

               tgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAgg                                                                          >  1:2770489/1‑93 (MQ=255)

               tgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAgg                                                                          >  1:677827/1‑93 (MQ=255)

               tgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAgg                                                                          >  2:3180473/1‑93 (MQ=255)

               tgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAgg                                                                          <  2:5062042/93‑1 (MQ=255)

                 atAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTc                                                                        <  2:1070837/93‑1 (MQ=255)

                  tAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCt                                                                       >  2:5824882/1‑93 (MQ=255)

                  tAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCt                                                                       >  1:5217834/1‑93 (MQ=255)

                      tGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCaa                                                                   <  1:4990315/93‑1 (MQ=255)

                      tGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCaa                                                                   >  1:4086313/1‑93 (MQ=255)

                      tGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCaa                                                                   >  1:1139053/1‑93 (MQ=255)

                       ggAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAAc                                                                  >  2:1809855/1‑93 (MQ=255)

                          aaaGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActgc                                                               >  2:3517463/1‑93 (MQ=255)

                          aaaGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActgc                                                               >  2:5190539/1‑93 (MQ=255)

                            aGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActgctg                                                             >  1:3666187/1‑93 (MQ=255)

                             gTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGa                                                            >  1:2803187/1‑93 (MQ=255)

                             gTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGa                                                            >  1:3910855/1‑93 (MQ=255)

                              tGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGAt                                                           <  2:5155976/93‑1 (MQ=255)

                                 gaCGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTaa                                                        >  2:4269781/1‑93 (MQ=255)

                                  gcGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAAc                                                       <  1:3606040/92‑1 (MQ=255)

                                   cGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACa                                                      >  2:172247/1‑93 (MQ=255)

                                    ggTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACaa                                                     <  2:569466/93‑1 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    >  2:2936676/1‑93 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    >  2:3675417/1‑93 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    >  1:4930074/1‑93 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    <  2:2720693/93‑1 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    >  2:6013319/1‑93 (MQ=255)

                                      tGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatc                                                   >  1:94592/1‑93 (MQ=255)

                                       gCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatca                                                  >  1:3051971/1‑93 (MQ=255)

                                       gCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatca                                                  <  1:4645688/93‑1 (MQ=255)

                                        cTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatcat                                                 >  2:2104598/1‑93 (MQ=255)

                                        cTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatcat                                                 >  1:5913253/1‑93 (MQ=255)

                                        cTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatcat                                                 <  2:605414/93‑1 (MQ=255)

                                         tGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatcatc                                                >  1:1044446/1‑93 (MQ=255)

                                         tGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatcatc                                                >  2:1658799/1‑93 (MQ=255)

                                         tGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatcatc                                                >  2:1217263/1‑93 (MQ=255)

                                          gTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCt                                               >  1:250051/1‑93 (MQ=255)

                                           ttttcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTg                                              <  1:2272928/90‑1 (MQ=255)

                                             ctcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaa                                            <  1:4277238/93‑1 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           >  1:796532/1‑93 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           >  1:4190867/1‑93 (MQ=255)

                                               ccGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAg                                          >  2:2825310/1‑93 (MQ=255)

                                                cGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGc                                         <  2:3552707/93‑1 (MQ=255)

                                                 gTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGca                                        <  2:3094003/93‑1 (MQ=255)

                                                   gCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGcaca                                      >  2:1022787/1‑93 (MQ=255)

                                                   gCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGcaca                                      >  2:4800576/1‑93 (MQ=255)

                                                   gCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGcaca                                      >  1:5749015/1‑93 (MQ=255)

                                                   gCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGcaca                                      <  2:5942940/93‑1 (MQ=255)

                                                   gCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGGCTGCAACTGCTGATTAACAATCATCTGAAAGcaca                                      >  2:3446791/1‑93 (MQ=255)

                                                    cAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCacac                                     <  1:4958268/93‑1 (MQ=255)

                                                    cAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAACCacac                                     >  2:5269835/1‑93 (MQ=255)

                                                     aGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACg                                    >  2:4310640/1‑93 (MQ=255)

                                                      ggATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGa                                   >  2:3300776/1‑93 (MQ=255)

                                                       gATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGat                                  <  1:3148893/93‑1 (MQ=255)

                                                        aTTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAACGCCCACGata                                 >  2:4692804/1‑93 (MQ=255)

                                                         tttAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGatat                                <  1:477634/93‑1 (MQ=255)

                                                          ttAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATg                               <  1:2959795/93‑1 (MQ=255)

                                                          ttAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATg                               <  1:3111274/93‑1 (MQ=255)

                                                           tAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATgc                              <  1:2666127/93‑1 (MQ=255)

                                                           tAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATgc                              <  2:1430415/93‑1 (MQ=255)

                                                             aTTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATgcgc                            >  1:2301295/1‑93 (MQ=255)

                                                                 atgaAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCcgcg                        <  2:2007247/93‑1 (MQ=255)

                                                                 atgaAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCcgcg                        >  2:1831453/1‑93 (MQ=255)

                                                                    aaGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCgcggcg                     >  2:4999864/1‑93 (MQ=255)

                                                                      gTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAt                   <  1:309347/93‑1 (MQ=255)

                                                                       ttGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATc                  <  2:5294340/93‑1 (MQ=255)

                                                                       ttGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATc                  <  2:4158840/93‑1 (MQ=255)

                                                                        tgtgCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATcg                 >  2:3250560/1‑93 (MQ=255)

                                                                         gtgCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATcgc                >  1:371222/1‑93 (MQ=255)

                                                                         gtgCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATcgc                >  2:11609/1‑93 (MQ=255)

                                                                         gtgCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATcgc                >  1:565159/1‑93 (MQ=255)

                                                                            ccTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGAt             <  1:2218408/93‑1 (MQ=255)

                                                                             cTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGAtc            >  2:2847686/1‑92 (MQ=255)

                                                                             cTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATg            <  1:3411577/93‑1 (MQ=255)

                                                                              ttCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGc           >  1:288805/1‑93 (MQ=255)

                                                                               tCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGcc          >  1:160863/1‑93 (MQ=255)

                                                                                cAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCt         <  1:3284918/93‑1 (MQ=255)

                                                                                   tgtTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACATTCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTac      <  2:3880762/93‑1 (MQ=255)

                                                                                   tgtTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTac      <  1:1951793/93‑1 (MQ=255)

                                                                                     ttGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTacga    <  2:3929699/93‑1 (MQ=255)

                                                                                     ttGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTacga    >  2:2573530/1‑93 (MQ=255)

                                                                                     ttGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTacga    >  1:351764/1‑93 (MQ=255)

                                                                                     ttGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTAATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTacga    >  1:608519/1‑93 (MQ=255)

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACG  >  NC_000913/2313043‑2313222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  NC_000913/2313042‑2313226

CGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGC                                                                                             >  SRR4302532.1249108/1‑94 (MQ=60)

 GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCC                                                                                            >  SRR4302532.3583713/1‑94 (MQ=60)

 GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCC                                                                                            >  SRR4302532.3218173/1‑94 (MQ=60)

  AACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCA                                                                                           <  SRR4302532.4856044/94‑1 (MQ=60)

  AACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGGGGTGTTTTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCA                                                                                           >  SRR4302532.3127387/1‑94 (MQ=60)

  AACATGCTTGCGGATGATAGCTGGAAAAGTGAGACCGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAATTTTGCCCGCCA                                                                                           >  SRR4302532.4994157/1‑94 (MQ=60)

    CATGCTTGCGGATGATAGCTGGCAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTCTTGCCCGCCATC                                                                                         >  SRR4302532.4494265/1‑94 (MQ=60)

    CATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATC                                                                                         <  SRR4302532.3090912/94‑1 (MQ=60)

    CATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATC                                                                                         >  SRR4302532.1828449/1‑94 (MQ=60)

    CATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATC                                                                                         >  SRR4302532.1773123/1‑94 (MQ=60)

     ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGCTGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        >  SRR4302532.3811663/1‑94 (MQ=60)

     ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        >  SRR4302532.4180158/1‑94 (MQ=60)

     ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        >  SRR4302532.234704/1‑94 (MQ=60)

     ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        <  SRR4302532.1725703/94‑1 (MQ=60)

     ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        >  SRR4302532.5797048/1‑94 (MQ=60)

        CTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGC                                                                                     >  SRR4302532.5008019/1‑94 (MQ=60)

        CTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGC                                                                                     >  SRR4302532.910181/1‑94 (MQ=60)

        CTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGC                                                                                     >  SRR4302532.1339585/1‑94 (MQ=60)

        CTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTCCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGC                                                                                     >  SRR4302532.1632101/1‑94 (MQ=60)

          TGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGT                                                                                   <  SRR4302532.146560/94‑1 (MQ=60)

           GCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTA                                                                                  <  SRR4302532.1928468/94‑1 (MQ=60)

            CGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAA                                                                                 >  SRR4302532.5002018/1‑94 (MQ=60)

            CGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAA                                                                                 >  SRR4302532.2342459/1‑94 (MQ=60)

            CGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAA                                                                                 >  SRR4302532.1960660/1‑94 (MQ=60)

             GGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAA                                                                                >  SRR4302532.3194114/1‑94 (MQ=60)

             GGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAA                                                                                >  SRR4302532.485662/1‑94 (MQ=60)

              GATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAG                                                                               >  SRR4302532.3211776/1‑94 (MQ=60)

              GATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAG                                                                               <  SRR4302532.1693688/94‑1 (MQ=60)

               ATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGG                                                                              >  SRR4302532.701137/1‑94 (MQ=60)

               ATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGG                                                                              >  SRR4302532.2863515/1‑94 (MQ=60)

                TGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGT                                                                             >  SRR4302532.2823353/1‑94 (MQ=60)

                  ATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCT                                                                           >  SRR4302532.5397787/1‑94 (MQ=60)

                  ATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCT                                                                           <  SRR4302532.4132228/94‑1 (MQ=60)

                    AGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGC                                                                         <  SRR4302532.4101343/94‑1 (MQ=60)

                     GCAGGAAAATTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCA                                                                        <  SRR4302532.6322510/94‑1 (MQ=60)

                      CTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAA                                                                       >  SRR4302532.1177027/1‑94 (MQ=60)

                      CTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAA                                                                       >  SRR4302532.4223483/1‑94 (MQ=60)

                       TGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAAC                                                                      <  SRR4302532.5161360/94‑1 (MQ=60)

                        GGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACT                                                                     <  SRR4302532.2395319/94‑1 (MQ=60)

                        GGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACT                                                                     <  SRR4302532.88786/94‑1 (MQ=60)

                         gaaattttagctGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCGCTTAGTGTTCCCCGCCATCATGCGTAAAGGTCTGCAACTG                                                                    <  SRR4302532.4403105/82‑1 (MQ=60)

                           AAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCT                                                                  <  SRR4302532.4746586/94‑1 (MQ=60)

                           AAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATGGAGGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCT                                                                  <  SRR4302532.2055726/94‑1 (MQ=60)

                            AAGTGAGTCGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCAACAAGCGTAAAGGTCTGCAACTGCTT                                                                 <  SRR4302532.1999152/94‑1 (MQ=60)

                            AAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTG                                                                 >  SRR4302532.3789326/1‑94 (MQ=60)

                            AAGGGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTG                                                                 <  SRR4302532.1999153/94‑1 (MQ=60)

                             AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                >  SRR4302532.2897097/1‑94 (MQ=60)

                             AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                >  SRR4302532.4042703/1‑94 (MQ=60)

                             AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                <  SRR4302532.3035852/94‑1 (MQ=60)

                             AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                <  SRR4302532.2701212/94‑1 (MQ=60)

                                 AGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAA                                                            >  SRR4302532.329233/1‑94 (MQ=60)

                                  GACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAAC                                                           <  SRR4302532.1924872/94‑1 (MQ=60)

                                   GCGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACA                                                          <  SRR4302532.3726571/94‑1 (MQ=60)

                                   ACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACA                                                          >  SRR4302532.6137941/1‑94 (MQ=60)

                                     GGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAAT                                                        >  SRR4302532.5099367/1‑94 (MQ=60)

                                     GGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAAT                                                        >  SRR4302532.885231/1‑94 (MQ=60)

                                      GTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATC                                                       >  SRR4302532.97889/1‑94 (MQ=60)

                                      GTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGACTGCAACTGCTGATTAACAATC                                                       >  SRR4302532.97887/1‑94 (MQ=60)

                                       TGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCA                                                      >  SRR4302532.3152726/1‑94 (MQ=60)

                                        GCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCAT                                                     <  SRR4302532.4804080/94‑1 (MQ=60)

                                        GCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCAT                                                     >  SRR4302532.6118027/1‑94 (MQ=60)

                                         CTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATC                                                    >  SRR4302532.1079653/1‑94 (MQ=60)

                                         CTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATC                                                    <  SRR4302532.5133987/94‑1 (MQ=60)

                                          TGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCT                                                   >  SRR4302532.258754/1‑94 (MQ=60)

                                           GTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTG                                                  <  SRR4302532.2804697/94‑1 (MQ=60)

                                            TTTTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGA                                                 <  SRR4302532.2348352/94‑1 (MQ=60)

                                            GTCTCCGTGCAGGTTTTAATTGTTGAAGTTGTGCCTTCAGTGTTGCCCGCCATCTAGCGTAATGGTCAGCAACTGCTGTTTTACAACCATCAGA                                                 >  SRR4302532.5676184/1‑94 (MQ=48)

                                             TCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAA                                                >  SRR4302532.2351158/1‑94 (MQ=60)

                                              CTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAA                                               >  SRR4302532.823773/1‑94 (MQ=60)

                                              CTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAA                                               >  SRR4302532.4331491/1‑94 (MQ=60)

                                              CTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAA                                               <  SRR4302532.4421256/94‑1 (MQ=60)

                                               TCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAG                                              <  SRR4302532.141063/94‑1 (MQ=60)

                                               TCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAG                                              <  SRR4302532.5450873/94‑1 (MQ=60)

                                                CCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGC                                             >  SRR4302532.1217788/1‑94 (MQ=60)

                                                   TGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACA                                          >  SRR4302532.5949131/1‑94 (MQ=60)

                                                     CAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACG                                        <  SRR4302532.5128351/94‑1 (MQ=60)

                                                     CAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACG                                        <  SRR4302532.5127689/94‑1 (MQ=60)

                                                     CAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACG                                        >  SRR4302532.6033532/1‑94 (MQ=60)

                                                     CAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACG                                        >  SRR4302532.1380091/1‑94 (MQ=60)

                                                        GATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATA                                     <  SRR4302532.1950593/94‑1 (MQ=60)

                                                        GATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATA                                     >  SRR4302532.5165742/1‑94 (MQ=60)

                                                        GATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATA                                     <  SRR4302532.3253364/94‑1 (MQ=60)

                                                         ATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATAT                                    <  SRR4302532.503303/94‑1 (MQ=60)

                                                          TTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATG                                   <  SRR4302532.494290/94‑1 (MQ=60)

                                                          TTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATG                                   <  SRR4302532.2934130/94‑1 (MQ=60)

                                                          TTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATG                                   <  SRR4302532.494289/94‑1 (MQ=60)

                                                          TTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATG                                   >  SRR4302532.3712968/1‑94 (MQ=60)

                                                           TTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGC                                  <  SRR4302532.3214482/94‑1 (MQ=60)

                                                           TTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGC                                  <  SRR4302532.3057836/94‑1 (MQ=60)

                                                            TAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCG                                 <  SRR4302532.2920931/94‑1 (MQ=60)

                                                            TAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCG                                 <  SRR4302532.2893442/94‑1 (MQ=60)

                                                            TAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCG                                 <  SRR4302532.2755615/94‑1 (MQ=60)

                                                             AATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGC                                >  SRR4302532.2377777/1‑94 (MQ=60)

                                                              ATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCC                               >  SRR4302532.184200/1‑94 (MQ=60)

                                                              ATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAAAACAAGATATGCGCC                               >  SRR4302532.5946625/1‑94 (MQ=60)

                                                               TTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCG                              <  SRR4302532.727029/94‑1 (MQ=60)

                                                                 GATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCG                            <  SRR4302532.3347837/94‑1 (MQ=60)

                                                                  ATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGG                           <  SRR4302532.6120439/94‑1 (MQ=60)

                                                                    gaaagtggGCCTTCAGGGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCG                         <  SRR4302532.4855441/86‑1 (MQ=60)

                                                                      AGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAT                       >  SRR4302532.4029210/1‑94 (MQ=60)

                                                                       GTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATC                      <  SRR4302532.320071/94‑1 (MQ=60)

                                                                         TGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGC                    >  SRR4302532.584872/1‑94 (MQ=60)

                                                                         TGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGC                    >  SRR4302532.384120/1‑94 (MQ=60)

                                                                          GTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCG                   >  SRR4302532.3764709/1‑94 (MQ=60)

                                                                            GCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGAT                 <  SRR4302532.3495728/94‑1 (MQ=60)

                                                                             CCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATG                <  SRR4302532.2292051/94‑1 (MQ=60)

                                                                              CTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGC               >  SRR4302532.298764/1‑94 (MQ=60)

                                                                              CTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGC               <  SRR4302532.3525834/94‑1 (MQ=60)

                                                                               TTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCC              <  SRR4302532.1113520/94‑1 (MQ=60)

                                                                               TTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCC              >  SRR4302532.4383557/1‑94 (MQ=60)

                                                                               TTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCC              >  SRR4302532.166212/1‑94 (MQ=60)

                                                                                 CAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTT            <  SRR4302532.422113/94‑1 (MQ=60)

                                                                                 CAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTT            <  SRR4302532.3394195/94‑1 (MQ=60)

                                                                                    TGTTGCCCGCTATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACG         <  SRR4302532.1270009/94‑1 (MQ=60)

                                                                                    TGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACG         <  SRR4302532.4228524/94‑1 (MQ=60)

                                                                                    TGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACG         <  SRR4302532.2016659/94‑1 (MQ=60)

                                                                                     GTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGA        >  SRR4302532.363943/1‑94 (MQ=60)

                                                                                     GTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTAATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGA        >  SRR4302532.629743/1‑94 (MQ=60)

                                                                                      TTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGAC       <  SRR4302532.5840718/94‑1 (MQ=60)

                                                                                        GCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGT     <  SRR4302532.3714364/94‑1 (MQ=60)

                                                                                          CCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTGT   >  SRR4302532.650910/1‑94 (MQ=60)

                                                                                          CCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTAT   >  SRR4302532.3699815/1‑94 (MQ=60)

                                                                                          CCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTAT   >  SRR4302532.3014777/1‑94 (MQ=60)

                                                                                           CGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  SRR4302532.5288912/1‑94 (MQ=60)

CGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  NC_000913/2313042‑2313226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`