Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,313,133	T→C	P542P (CCT→CCC)	yojN →	putative 2‑component sensor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,313,133	0	T	C	99.6%	835.3 / ‑6.8	226	P542P (CCT→CCC)	yojN	putative 2‑component sensor protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (109/116); total (109/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACG  >  NC_000913/2313043‑2313222

gAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTtgcccgc                                                                                         >  2:1836751/1‑93 (MQ=255)

 aaCATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTtgcccgcc                                                                                        >  1:1032169/1‑93 (MQ=255)

 aaCATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTtgcccgcc                                                                                        >  1:5366105/1‑93 (MQ=255)

  aCATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCa                                                                                       >  1:424975/1‑93 (MQ=255)

     tGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGtcatca                                                                                    >  2:5631469/1‑93 (MQ=255)

      gCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCaa                                                                                   <  2:3407532/93‑1 (MQ=255)

      gCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCaa                                                                                   <  2:5737284/93‑1 (MQ=255)

      gCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCaa                                                                                   <  2:4942117/93‑1 (MQ=255)

       cTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAg                                                                                  <  1:3366535/93‑1 (MQ=255)

        ttGCGGTTGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGc                                                                                 >  1:5520433/1‑93 (MQ=255)

        ttGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGc                                                                                 <  2:1028338/93‑1 (MQ=255)

            ggATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaa                                                                             <  2:6323048/93‑1 (MQ=255)

            ggATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaa                                                                             >  2:4766394/1‑93 (MQ=255)

             gatgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaaa                                                                            >  1:5149455/1‑93 (MQ=255)

             gacgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTaaa                                                                            >  1:815832/4‑93 (MQ=255)

              atgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAg                                                                           >  2:5295310/1‑93 (MQ=255)

               tgatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAgg                                                                          <  1:2654739/93‑1 (MQ=255)

                gatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGt                                                                         >  1:6474905/1‑93 (MQ=255)

                gatAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGt                                                                         >  1:3533503/1‑93 (MQ=255)

                 atAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTc                                                                        >  1:1758120/1‑93 (MQ=255)

                 atAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTc                                                                        <  1:5064515/93‑1 (MQ=255)

                  tAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCt                                                                       <  2:6395033/93‑1 (MQ=255)

                     cTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCa                                                                    <  2:4064678/93‑1 (MQ=255)

                     cTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCa                                                                    <  2:2148967/93‑1 (MQ=255)

                      tGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCaa                                                                   <  2:1099250/93‑1 (MQ=255)

                       ggAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCagc                                                                  >  2:2213445/1‑91 (MQ=255)

                        gAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAAct                                                                 >  1:5253744/1‑93 (MQ=255)

                         aaaaGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActg                                                                <  2:5207898/93‑1 (MQ=255)

                          aaaGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActgc                                                               >  2:1785188/1‑93 (MQ=255)

                          aaaGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActgc                                                               <  1:5218509/93‑1 (MQ=255)

                           aaGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAActgct                                                              <  1:1916977/93‑1 (MQ=255)

                             gTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGa                                                            <  2:2375571/93‑1 (MQ=255)

                             gTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGa                                                            <  1:3726294/93‑1 (MQ=255)

                             gTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGa                                                            >  1:2350931/1‑93 (MQ=255)

                                 gaCGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTaa                                                        <  2:1396735/93‑1 (MQ=255)

                                  aCGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAAc                                                       >  2:2880604/1‑93 (MQ=255)

                                  aCGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAAc                                                       <  1:2692643/93‑1 (MQ=255)

                                   cGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACa                                                      <  2:4602352/93‑1 (MQ=255)

                                   cGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACa                                                      >  1:1216528/1‑93 (MQ=255)

                                   cGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACa                                                      <  2:3640179/93‑1 (MQ=255)

                                    ggTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACaa                                                     <  2:595794/93‑1 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    >  2:4076510/1‑93 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    <  1:5394899/93‑1 (MQ=255)

                                     gTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAat                                                    >  1:6593872/1‑93 (MQ=255)

                                      tGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatc                                                   >  1:5588803/1‑93 (MQ=255)

                                       gCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAatca                                                  <  1:152611/93‑1 (MQ=255)

                                          gTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCt                                               <  2:4013441/93‑1 (MQ=255)

                                          gTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCt                                               >  2:1245434/1‑93 (MQ=255)

                                          gTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCt                                               >  1:2560197/1‑93 (MQ=255)

                                           ttCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTg                                              >  1:3181267/1‑93 (MQ=255)

                                            tctcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGa                                             >  1:3241412/1‑93 (MQ=255)

                                            tctcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGa                                             <  2:3527290/93‑1 (MQ=255)

                                            tctcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGa                                             <  2:6254308/93‑1 (MQ=255)

                                             ctcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaa                                            >  2:4721203/1‑93 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           >  2:6119720/1‑93 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           >  2:5478119/1‑93 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           <  2:128194/93‑1 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           >  1:6434399/1‑93 (MQ=255)

                                              tcCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGaaa                                           <  1:3036659/93‑1 (MQ=255)

                                                cGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGc                                         <  2:4382599/93‑1 (MQ=255)

                                                cGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGc                                         <  2:3785228/93‑1 (MQ=255)

                                                 gTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGca                                        >  1:2070090/1‑93 (MQ=255)

                                                  tGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGcac                                       <  1:2419615/93‑1 (MQ=255)

                                                     aGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACg                                    >  1:697077/1‑93 (MQ=255)

                                                      ggATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGa                                   <  2:6573846/93‑1 (MQ=255)

                                                       gATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGat                                  <  1:1135058/93‑1 (MQ=255)

                                                       gATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGat                                  >  2:5923376/1‑93 (MQ=255)

                                                       gATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGat                                  <  2:2549387/93‑1 (MQ=255)

                                                       gATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGat                                  >  1:4947552/1‑93 (MQ=255)

                                                        aTTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGata                                 >  1:412656/1‑93 (MQ=255)

                                                        aTTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGata                                 <  2:3870192/93‑1 (MQ=255)

                                                         tttAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGatat                                <  1:4311212/93‑1 (MQ=255)

                                                         tttAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGatat                                >  1:1514091/1‑93 (MQ=255)

                                                           tAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATgc                              >  2:249000/1‑93 (MQ=255)

                                                           tAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATgc                              >  1:1370603/1‑93 (MQ=255)

                                                            aaTTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATgcg                             >  2:2773196/1‑93 (MQ=255)

                                                               tgatgaAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGcgccg                          >  1:171356/1‑93 (MQ=255)

                                                                gatgaAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGcgccgc                         >  1:6137671/1‑93 (MQ=255)

                                                                     aGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGa                    <  2:5159739/93‑1 (MQ=255)

                                                                     aGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGa                    <  2:5556498/93‑1 (MQ=255)

                                                                      gTTGTGCCTTCAGTGTTGCCCGCCATCACGCGTAAAGGTCTGCAACTGCTGATTACCAATCACCTGACAGCACACGATATGCGCCGCGGCGAt                   <  1:5701252/93‑1 (MQ=255)

                                                                      gTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAt                   <  2:1339300/93‑1 (MQ=255)

                                                                      gTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAt                   >  1:4689939/1‑93 (MQ=255)

                                                                      gTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAt                   >  1:3794790/1‑93 (MQ=255)

                                                                        tgtgCCTGCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATcg                 >  1:6311887/1‑93 (MQ=255)

                                                                         gtgCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATcgc                <  2:23557/93‑1 (MQ=255)

                                                                           gCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGa              <  2:2443072/93‑1 (MQ=255)

                                                                           gCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGa              >  2:3123937/1‑93 (MQ=255)

                                                                             cTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATg            <  1:1827412/93‑1 (MQ=255)

                                                                              ttCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGc           >  2:1656750/1‑93 (MQ=255)

                                                                              ttCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGc           >  2:1767368/1‑93 (MQ=255)

                                                                              ttCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGc           <  1:3349484/93‑1 (MQ=255)

                                                                                cAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCt         >  1:1310117/1‑93 (MQ=255)

                                                                                cAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCt         <  2:424975/93‑1 (MQ=255)

                                                                                 aGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCtt        >  2:1998117/1‑93 (MQ=255)

                                                                                  gtgtTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTa       <  1:5911902/93‑1 (MQ=255)

                                                                                  gtgtTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTa       <  1:489985/93‑1 (MQ=255)

                                                                                   tgtTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTac      <  2:4343575/93‑1 (MQ=255)

                                                                                     ttGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTacga    <  2:1578926/93‑1 (MQ=255)

                                                                                     ttGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTacga    >  2:684482/1‑93 (MQ=255)

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACG  >  NC_000913/2313043‑2313222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  NC_000913/2313043‑2313226

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCC                                                                                            >  SRR4302547.1052462/1‑94 (MQ=60)

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCC                                                                                            >  SRR4302547.5476825/1‑94 (MQ=60)

 AACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCA                                                                                           >  SRR4302547.432850/1‑94 (MQ=60)

 AACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCA                                                                                           >  SRR4302547.5416583/1‑94 (MQ=60)

  ACATGCTTGCGGAGGATAGCTGGAAAAGTGAGACGGTGCTTTGCGCCGTGCAGGATTTAATTGATGAAGTTGTTCCTTCAGTGTTGCCCGCCAT                                                                                          <  SRR4302547.1162406/94‑1 (MQ=60)

    ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        <  SRR4302547.4842794/94‑1 (MQ=60)

    ATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCA                                                                                        <  SRR4302547.1555030/94‑1 (MQ=60)

     TGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAA                                                                                       <  SRR4302547.3660893/94‑1 (MQ=60)

       CTTGCGGTTGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGC                                                                                     >  SRR4302547.5634392/1‑94 (MQ=60)

       CTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGC                                                                                     <  SRR4302547.3434910/94‑1 (MQ=60)

            GGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAA                                                                                >  SRR4302547.4619475/1‑94 (MQ=60)

            GGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAA                                                                                >  SRR4302547.5255504/1‑94 (MQ=60)

            GGACGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAA                                                                                >  SRR4302547.831324/1‑94 (MQ=60)

             GATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAG                                                                               >  SRR4302547.2821648/1‑94 (MQ=60)

             GATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAG                                                                               >  SRR4302547.1553362/1‑94 (MQ=60)

              ATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGG                                                                              >  SRR4302547.6685312/1‑94 (MQ=60)

               TGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGT                                                                             <  SRR4302547.2708096/94‑1 (MQ=60)

               TGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGT                                                                             >  SRR4302547.3605658/1‑94 (MQ=60)

               TGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGT                                                                             >  SRR4302547.4170448/1‑94 (MQ=60)

               TGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGT                                                                             >  SRR4302547.6609719/1‑94 (MQ=60)

                GATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTC                                                                            <  SRR4302547.2543971/94‑1 (MQ=60)

                GATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTC                                                                            >  SRR4302547.1792939/1‑94 (MQ=60)

                 ATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCT                                                                           <  SRR4302547.5169025/94‑1 (MQ=60)

                   AGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGC                                                                         >  SRR4302547.5118658/1‑94 (MQ=60)

                    GCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCA                                                                        >  SRR4302547.2044739/1‑94 (MQ=60)

                    GCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCA                                                                        <  SRR4302547.6765530/94‑1 (MQ=60)

                       GGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACT                                                                     >  SRR4302547.5361921/1‑94 (MQ=60)

                          AAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCT                                                                  <  SRR4302547.5325920/94‑1 (MQ=60)

                           AAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTG                                                                 <  SRR4302547.1954939/94‑1 (MQ=60)

                           AAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTG                                                                 <  SRR4302547.3824217/94‑1 (MQ=60)

                            AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                >  SRR4302547.2398577/1‑94 (MQ=60)

                            AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                >  SRR4302547.868257/1‑94 (MQ=60)

                            AGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGA                                                                >  SRR4302547.6141735/1‑94 (MQ=60)

                             GTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGAT                                                               <  SRR4302547.3802360/94‑1 (MQ=60)

                              TGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATT                                                              >  SRR4302547.2745415/1‑94 (MQ=60)

                               GAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTA                                                             >  SRR4302547.828168/1‑94 (MQ=60)

                               GAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTA                                                             <  SRR4302547.3431254/94‑1 (MQ=60)

                               GAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTA                                                             <  SRR4302547.637145/94‑1 (MQ=60)

                                AGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAA                                                            >  SRR4302547.11035/1‑94 (MQ=60)

                                AGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAA                                                            <  SRR4302547.3117571/94‑1 (MQ=60)

                                 GACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAAC                                                           <  SRR4302547.97429/94‑1 (MQ=60)

                                  ACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACA                                                          <  SRR4302547.2746695/94‑1 (MQ=60)

                                  ACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACA                                                          >  SRR4302547.1241086/1‑94 (MQ=60)

                                   CGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAA                                                         <  SRR4302547.5494319/94‑1 (MQ=60)

                                   CGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAA                                                         >  SRR4302547.5802695/1‑94 (MQ=60)

                                    GGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAAT                                                        >  SRR4302547.6731154/1‑94 (MQ=60)

                                     GTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATC                                                       <  SRR4302547.5506349/94‑1 (MQ=60)

                                     GTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATC                                                       >  SRR4302547.5704089/1‑94 (MQ=60)

                                       GCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCAT                                                     <  SRR4302547.155393/94‑1 (MQ=60)

                                        CTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATC                                                    >  SRR4302547.1493278/1‑94 (MQ=60)

                                         TGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCT                                                   >  SRR4302547.2611897/1‑94 (MQ=60)

                                          GTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTG                                                  >  SRR4302547.3244666/1‑94 (MQ=60)

                                           TTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGA                                                 >  SRR4302547.3306107/1‑94 (MQ=60)

                                             CTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAA                                               >  SRR4302547.6568509/1‑94 (MQ=60)

                                             CTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAA                                               >  SRR4302547.5049398/1‑94 (MQ=60)

                                              TCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAG                                              <  SRR4302547.3097074/94‑1 (MQ=60)

                                              TCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAG                                              >  SRR4302547.460730/1‑94 (MQ=60)

                                                CGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCA                                            <  SRR4302547.5349247/94‑1 (MQ=60)

                                                CGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCA                                            >  SRR4302547.2111414/1‑94 (MQ=60)

                                                  TGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACC                                          >  SRR4302547.1279339/1‑94 (MQ=60)

                                                  TGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACA                                          <  SRR4302547.2468597/94‑1 (MQ=60)

                                                  TGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACA                                          >  SRR4302547.3546685/1‑94 (MQ=60)

                                                  TGCAGGATTTAATTGATGAAGTTGTGCATTCAGTGTTTCCCGCCATCAAGCGTAAAGGTCTCCAACTGCTGCTTAACAATCATCTGAAAGCACA                                          >  SRR4302547.5322599/1‑94 (MQ=60)

                                                  TGCAGGATTTAATTGATGAAGGTGTGCCTTCAGTGTTGTCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACA                                          >  SRR4302547.1782620/1‑94 (MQ=60)

                                                    CAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACG                                        >  SRR4302547.709964/1‑94 (MQ=60)

                                                      GGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGAT                                      >  SRR4302547.5050010/1‑94 (MQ=60)

                                                       GATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATA                                     <  SRR4302547.1157979/94‑1 (MQ=60)

                                                       GATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATA                                     >  SRR4302547.420313/1‑94 (MQ=60)

                                                        ATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATAT                                    >  SRR4302547.1544264/1‑94 (MQ=60)

                                                         TTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATG                                   <  SRR4302547.4399904/94‑1 (MQ=60)

                                                          TTAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGC                                  >  SRR4302547.1398103/1‑94 (MQ=60)

                                                           TAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCG                                 <  SRR4302547.6558985/94‑1 (MQ=60)

                                                           TAATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCG                                 >  SRR4302547.3320215/1‑94 (MQ=60)

                                                             ATTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCA                               >  SRR4302547.1275253/1‑94 (MQ=60)

                                                              TTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCG                              <  SRR4302547.3614323/94‑1 (MQ=60)

                                                              TTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCG                              >  SRR4302547.2332814/1‑94 (MQ=60)

                                                              TTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCG                              <  SRR4302547.3277594/94‑1 (MQ=60)

                                                              TTGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCG                              >  SRR4302547.174492/1‑94 (MQ=60)

                                                               TGATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGC                             >  SRR4302547.6264991/1‑94 (MQ=60)

                                                                GATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCG                            <  SRR4302547.5735865/94‑1 (MQ=60)

                                                                 ATGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGG                           >  SRR4302547.3312735/1‑94 (MQ=60)

                                                                  TGAAGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGC                          <  SRR4302547.2671282/94‑1 (MQ=60)

                                                                     AGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAT                       >  SRR4302547.3872153/1‑94 (MQ=60)

                                                                     AGTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGAT                       >  SRR4302547.4787069/1‑94 (MQ=60)

                                                                      GTTGTGCCTTCAGTGTTGCCCGCCATCACGCGTAAAGGTCTGCAACTGCTGATTACCAATCACCTGACAGCACACGATATGCGCCGCGGCGATC                      <  SRR4302547.5819014/94‑1 (MQ=60)

                                                                      GTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATC                      <  SRR4302547.5133678/94‑1 (MQ=60)

                                                                      GTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATC                      <  SRR4302547.2896721/94‑1 (MQ=60)

                                                                      GTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATC                      <  SRR4302547.5338001/94‑1 (MQ=60)

                                                                      GTTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATC                      >  SRR4302547.2415825/1‑94 (MQ=60)

                                                                      GTTGTGCCTTCAGTGTTGCCCGACATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATC                      >  SRR4302547.2509128/1‑94 (MQ=60)

                                                                       TTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCG                     <  SRR4302547.1379233/94‑1 (MQ=60)

                                                                       TTGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCG                     <  SRR4302547.5205418/94‑1 (MQ=60)

                                                                       TTGTGCCTGCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCG                     >  SRR4302547.6443118/1‑94 (MQ=60)

                                                                        TGTGCCTTCCGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGC                    >  SRR4302547.6600309/1‑94 (MQ=60)

                                                                        TGTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGC                    >  SRR4302547.5540593/1‑94 (MQ=60)

                                                                         GTGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCG                   >  SRR4302547.635707/1‑94 (MQ=60)

                                                                          TGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGA                  <  SRR4302547.2389744/94‑1 (MQ=60)

                                                                          TGCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGA                  <  SRR4302547.6620103/94‑1 (MQ=60)

                                                                           GCCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGAT                 <  SRR4302547.5615193/94‑1 (MQ=60)

                                                                            CCTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATG                >  SRR4302547.4278875/1‑94 (MQ=60)

                                                                             CTTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGC               <  SRR4302547.1863532/94‑1 (MQ=60)

                                                                              TTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCC              >  SRR4302547.6025259/1‑94 (MQ=60)

                                                                              TTCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCC              <  SRR4302547.3417400/94‑1 (MQ=60)

                                                                               TCAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCT             >  SRR4302547.1336466/1‑94 (MQ=60)

                                                                                CAGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTT            <  SRR4302547.1835918/94‑1 (MQ=60)

                                                                                 AGTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTA           >  SRR4302547.1922061/1‑94 (MQ=60)

                                                                                  GTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTAC          <  SRR4302547.6034351/94‑1 (MQ=60)

                                                                                  GTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTAC          <  SRR4302547.498936/94‑1 (MQ=60)

                                                                                  GTGTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTAC          <  SRR4302547.4093530/94‑1 (MQ=60)

                                                                                    GTTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGA        <  SRR4302547.1578631/94‑1 (MQ=60)

                                                                                     TTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGAC       <  SRR4302547.4374380/94‑1 (MQ=60)

                                                                                     TTGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGAC       <  SRR4302547.1816010/94‑1 (MQ=60)

                                                                                      TGCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACG      >  SRR4302547.5671267/1‑94 (MQ=60)

                                                                                       GCCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGT     >  SRR4302547.4660951/1‑94 (MQ=60)

                                                                                        CCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTA    >  SRR4302547.2014382/1‑94 (MQ=60)

                                                                                        CCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTA    >  SRR4302547.3848147/1‑94 (MQ=60)

                                                                                        CCCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTA    >  SRR4302547.2448269/1‑94 (MQ=60)

                                                                                         CCGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTAT   >  SRR4302547.6760909/1‑94 (MQ=60)

                                                                                          CGCCATCAGGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  <  SRR4302547.3771385/94‑1 (MQ=60)

                                                                                          CGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  SRR4302547.2909805/1‑94 (MQ=60)

                                                                                          CGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  SRR4302547.4202092/1‑94 (MQ=60)

GAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTAAAGGTCTGCAACTGCTGATTAACAATCATCTGAAAGCACACGATATGCGCCGCGGCGATCGCGATGCCTTACGACGTATT  >  NC_000913/2313043‑2313226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`