Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,896,819	+C	coding (622/657 nt)	ygcU ←	putative oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,896,818	1	.	C	99.6%	889.0 / ‑3.8	246	coding (623/657 nt)	ygcU	putative oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (160/85); total (160/86)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 263 total aligned reads displayed.

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCT  >  NC_000913/2896726‑2896904

   aGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccg                                                                                     >  1:2096857/1‑91 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                    >  2:3469981/1‑90 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                    >  2:2630888/1‑90 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                    >  2:754873/1‑90 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                    >  1:2346973/1‑90 (MQ=255)

     gATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccgat                                                                                   <  1:2049023/93‑5 (MQ=255)

       tataGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatga                                                                                  >  2:4809582/1‑93 (MQ=255)

       aataGGCGTAGAAGAGTTTGTTTTGCGGGGTGTATTTAAAACTTTTCACTGTTACTACAGTGATATAGCACAATGCACCTTCGTTGCCgatga                                                                                  <  1:549626/92‑1 (MQ=255)

         taGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatgatg                                                                                >  2:5328658/1‑93 (MQ=255)

         taGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatgatg                                                                                >  2:1291472/1‑93 (MQ=255)

         taGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatgatg                                                                                <  1:3733652/93‑1 (MQ=255)

          aGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatgatga                                                                               <  2:5690230/93‑1 (MQ=255)

          aGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatgatga                                                                               <  2:4727234/93‑1 (MQ=255)

          aGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCgatgatga                                                                               >  1:876868/1‑93 (MQ=255)

            ccGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGatgatgatg                                                                             >  2:3710011/1‑93 (MQ=255)

             cGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCATTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGACGATGATGAtgt                                                                            >  2:2586206/1‑93 (MQ=255)

             cGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGAtgt                                                                            <  2:862194/93‑1 (MQ=255)

               tAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGa                                                                          >  2:1615515/1‑93 (MQ=255)

               tAGAAGAGGTTGTTTTCCGGGGCGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGa                                                                          >  2:4519604/1‑93 (MQ=255)

                agaagaGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGAc                                                                         >  1:2865482/1‑93 (MQ=255)

                   agagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGaa                                                                      <  2:5895191/93‑1 (MQ=255)

                    gagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAAt                                                                     >  2:2280210/1‑93 (MQ=255)

                    gagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAAt                                                                     >  1:1237765/1‑93 (MQ=255)

                        ttgttTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTcc                                                                 <  2:4024632/93‑1 (MQ=255)

                         tgttTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCg                                                                >  2:4628875/1‑93 (MQ=255)

                           ttttCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCggg                                                              >  2:1925549/1‑93 (MQ=255)

                            tttCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGc                                                             >  1:1148648/1‑93 (MQ=255)

                             ttCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGcc                                                            <  1:4213581/93‑1 (MQ=255)

                             ttCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGcc                                                            <  2:3805168/93‑1 (MQ=255)

                              tCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCa                                                           <  1:5721069/93‑1 (MQ=255)

                                 ggggTGAATTTAAAGATTTTTACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGcc                                                        >  1:1269200/1‑93 (MQ=255)

                                  gggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCcg                                                       >  1:3548821/1‑93 (MQ=255)

                                   ggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCcgc                                                      >  1:5077758/1‑93 (MQ=255)

                                    gTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGGCCGGGCCAGCcgcg                                                     >  1:5945739/1‑93 (MQ=255)

                                     tGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCcgcgc                                                    <  2:3602080/93‑1 (MQ=255)

                                       aaTTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCgcgg                                                  >  1:591118/1‑93 (MQ=255)

                                       aaTTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCgcgg                                                  >  2:2857220/1‑93 (MQ=255)

                                        aTTTAAAGATTTTCACTGTTACTTGAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGGCAGCCGCgcggg                                                 >  1:492404/1‑92 (MQ=255)

                                        aTTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCgcggc                                                 >  2:2734830/1‑93 (MQ=255)

                                         tttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGG‑CAGCCGCGCGGCGt                                               >  2:2817775/1‑93 (MQ=255)

                                          ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGt                                               <  2:5164375/93‑1 (MQ=255)

                                          ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGt                                               <  1:1072862/93‑1 (MQ=255)

                                           tAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCgcggcgcg                                              >  1:3315803/1‑91 (MQ=255)

                                           tAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTg                                              >  1:3185890/1‑93 (MQ=255)

                                             aaGATTTTCACTGTTACTTCCGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGc                                            <  1:4060286/93‑1 (MQ=255)

                                              aGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCa                                           >  2:1018079/1‑93 (MQ=255)

                                              aGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCa                                           >  2:1519994/1‑93 (MQ=255)

                                               gATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCAc                                          <  2:3187815/93‑1 (MQ=255)

                                                 ttttCACTGTTCCTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGt                                        <  2:3430127/93‑1 (MQ=255)

                                                 ttttCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGt                                        >  1:5343818/1‑93 (MQ=255)

                                                   ttCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGttt                                      <  2:5402284/93‑1 (MQ=255)

                                                   ttCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGttt                                      >  2:4785734/1‑93 (MQ=255)

                                                       cTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCCCGTTTTTat                                  >  1:2555037/1‑92 (MQ=255)

                                                       cTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTaa                                  >  1:852549/1‑93 (MQ=255)

                                                       cTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTaa                                  <  1:3467449/93‑1 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAt                                 >  2:313460/1‑93 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAACCGCGCGGCGTGGCACGTTTTTAAt                                 >  1:2644151/1‑93 (MQ=255)

                                                          ttACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGc                               >  2:4332189/1‑93 (MQ=255)

                                                          ttACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGc                               >  1:64422/1‑93 (MQ=255)

                                                           tACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCg                              >  1:3610013/1‑93 (MQ=255)

                                                           tACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCg                              <  1:3098678/93‑1 (MQ=255)

                                                            aCTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgt                             >  1:6106786/1‑93 (MQ=255)

                                                             cTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgtg                            <  2:20800/93‑1 (MQ=255)

                                                             cTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgtg                            >  1:2379120/1‑93 (MQ=255)

                                                              ttCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgtgt                           <  2:2034525/93‑1 (MQ=255)

                                                               tCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGtgtg                          >  2:4193063/1‑93 (MQ=255)

                                                                cAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGa                         >  2:2374630/1‑93 (MQ=255)

                                                                cAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGa                         >  2:16419/1‑93 (MQ=255)

                                                                 aGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGAc                        <  1:1374262/93‑1 (MQ=255)

                                                                  gTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACg                       >  1:5646482/1‑93 (MQ=255)

                                                                   tGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACgg                      <  2:3284616/93‑1 (MQ=255)

                                                                   ggATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACgg                      <  2:1050430/92‑1 (MQ=255)

                                                                    gATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACggg                     >  2:4993081/1‑92 (MQ=255)

                                                                    gATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGt                     >  2:4113872/1‑93 (MQ=255)

                                                                      tataGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGc                   <  1:3935371/93‑1 (MQ=255)

                                                                       ataGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGcc                  >  1:4348596/1‑93 (MQ=255)

                                                                        taGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCa                 >  2:2446632/1‑93 (MQ=255)

                                                                        taGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCa                 <  2:1159407/93‑1 (MQ=255)

                                                                         aGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCAt                <  1:3263116/93‑1 (MQ=255)

                                                                          gCACAATGCTCCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATc               <  2:4383525/93‑1 (MQ=255)

                                                                          gCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATc               >  2:6117321/1‑93 (MQ=255)

                                                                            acaATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAg             >  1:5145749/1‑93 (MQ=255)

                                                                            acaATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAg             <  1:1968163/93‑1 (MQ=255)

                                                                            acaATGCACCTTCGTTGCCGATGAAGATATGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAg             <  2:5469277/93‑1 (MQ=255)

                                                                             caATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGc            >  2:5054699/1‑93 (MQ=255)

                                                                                tGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCaa         <  2:1724376/93‑1 (MQ=255)

                                                                                tGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCaa         <  1:3790997/93‑1 (MQ=255)

                                                                                 gCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAAt        <  2:5605262/93‑1 (MQ=255)

                                                                                  cACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATa       >  1:941709/1‑93 (MQ=255)

                                                                                  cACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATa       >  1:1578646/1‑93 (MQ=255)

                                                                                  cACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATa       <  1:5430559/93‑1 (MQ=255)

                                                                                  cACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATa       <  2:1988914/93‑1 (MQ=255)

                                                                                    ccTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACt     >  1:4698007/1‑93 (MQ=255)

                                                                                    ccTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAACACt     >  1:299303/1‑93 (MQ=255)

                                                                                     cTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTg    >  1:4528791/1‑93 (MQ=255)

                                                                                     cTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTg    >  1:4570223/1‑93 (MQ=255)

                                                                                     cTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTg    <  1:6029922/93‑1 (MQ=255)

                                                                                      ttCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGc   >  1:1766895/1‑93 (MQ=255)

                                                                                      ttCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGc   >  2:1303999/1‑93 (MQ=255)

                                                                                       tCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCt  >  2:459186/1‑93 (MQ=255)

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCT  >  NC_000913/2896726‑2896904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACC  >  NC_000913/2896727‑2896912

CCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑CC                                                                                              >  SRR4302532.5265861/1‑94 (MQ=60)

 CAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑Ccg                                                                                             >  SRR4302532.3743999/1‑92 (MQ=60)

 CAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑Ccg                                                                                             >  SRR4302532.2166801/1‑92 (MQ=60)

  AGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑Ccga                                                                                            >  SRR4302532.2425245/1‑91 (MQ=60)

  AGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑Ccga                                                                                            >  SRR4302532.1693936/1‑91 (MQ=60)

    GATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATG                                                                                           >  SRR4302532.1016245/1‑94 (MQ=60)

    GATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATG                                                                                           <  SRR4302532.2117252/94‑1 (MQ=60)

     ATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGA                                                                                          >  SRR4302532.6079705/1‑94 (MQ=60)

      AATAGGCGTAGAAGAGTTTGTTTTGCGGGGTGTATTTAAAACTTTTCACTGTTACTACAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGAT                                                                                         <  SRR4302532.568798/94‑1 (MQ=57)

       ATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATG                                                                                        <  SRR4302532.1886197/94‑1 (MQ=60)

        TAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGA                                                                                       <  SRR4302532.3859084/94‑1 (MQ=60)

        TAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGA                                                                                       >  SRR4302532.906863/1‑94 (MQ=60)

         AGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGAT                                                                                      >  SRR4302532.2410061/1‑94 (MQ=60)

         AGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGAT                                                                                      >  SRR4302532.2033773/1‑94 (MQ=60)

           CCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGT                                                                                    >  SRR4302532.1932629/1‑94 (MQ=60)

            CGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTG                                                                                   >  SRR4302532.3636083/1‑94 (MQ=60)

            CGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTG                                                                                   >  SRR4302532.4097613/1‑94 (MQ=60)

              TAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGAC                                                                                 >  SRR4302532.2961321/1‑94 (MQ=60)

               AGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACG                                                                                >  SRR4302532.3528382/1‑94 (MQ=60)

               AGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACG                                                                                <  SRR4302532.5650252/94‑1 (MQ=60)

                GAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGA                                                                               >  SRR4302532.4295659/1‑94 (MQ=60)

                GAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGA                                                                               >  SRR4302532.5630160/1‑94 (MQ=60)

                  AGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAAT                                                                             <  SRR4302532.3202811/94‑1 (MQ=60)

                  AGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAAT                                                                             >  SRR4302532.1279168/1‑94 (MQ=60)

                   GAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATG                                                                            >  SRR4302532.939929/1‑94 (MQ=60)

                     GGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTC                                                                          >  SRR4302532.6130974/1‑94 (MQ=60)

                      GTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCC                                                                         >  SRR4302532.4818600/1‑94 (MQ=60)

                      GTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCC                                                                         <  SRR4302532.390261/94‑1 (MQ=60)

                        TGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAACGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGG                                                                       <  SRR4302532.3910854/94‑1 (MQ=60)

                          TTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGC                                                                     >  SRR4302532.6328810/1‑94 (MQ=60)

                          TTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGC                                                                     >  SRR4302532.1186994/1‑94 (MQ=60)

                            TTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCA                                                                   <  SRR4302532.4355011/94‑1 (MQ=60)

                            TTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCA                                                                   >  SRR4302532.6264961/1‑94 (MQ=60)

                             TCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAG                                                                  <  SRR4302532.5920350/94‑1 (MQ=60)

                              CCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGC                                                                 >  SRR4302532.545434/1‑94 (MQ=60)

                               CGTGGTGAATTGAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTG‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCC                                                                <  SRR4302532.2980498/94‑1 (MQ=60)

                               CGGGGTGAATTTAAAGATTTTTACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCC                                                                >  SRR4302532.1311660/1‑94 (MQ=60)

                                GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCG                                                               >  SRR4302532.3667560/1‑94 (MQ=60)

                                GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCG                                                               >  SRR4302532.3833718/1‑94 (MQ=60)

                                 GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302532.96092/1‑94 (MQ=60)

                                 GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302532.3340190/1‑94 (MQ=60)

                                 GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302532.5252328/1‑94 (MQ=60)

                                 GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302532.2657465/1‑94 (MQ=60)

                                 GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302532.5958072/1‑94 (MQ=60)

                                  GGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGGCCGGGCCAGCCGCG                                                             >  SRR4302532.6151452/1‑94 (MQ=60)

                                   GTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGC                                                            >  SRR4302532.1286489/1‑94 (MQ=60)

                                     GAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGG                                                          >  SRR4302532.3140942/1‑94 (MQ=60)

                                     GAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGG                                                          >  SRR4302532.611744/1‑94 (MQ=60)

                                      AATTTAAAGATTTTCATTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTAC‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGC                                                         <  SRR4302532.1439162/94‑1 (MQ=60)

                                      AATTTAAAGATTTTCACTGTTACTTGAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGGCAGCCGCGCGGG                                                         >  SRR4302532.509607/1‑94 (MQ=60)

                                       ATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCG                                                        >  SRR4302532.5310636/1‑94 (MQ=60)

                                       ATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCG                                                        >  SRR4302532.5860801/1‑94 (MQ=60)

                                       ATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCG                                                        <  SRR4302532.3667309/94‑1 (MQ=60)

                                        TTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCGCCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGT                                                       <  SRR4302532.1619720/94‑1 (MQ=60)

                                         TTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTG                                                      <  SRR4302532.1108890/94‑1 (MQ=60)

                                         TTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTG                                                      >  SRR4302532.5888073/1‑94 (MQ=60)

                                         TTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTG                                                      >  SRR4302532.3291664/1‑94 (MQ=60)

                                         TTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGCG                                                      >  SRR4302532.3426361/1‑94 (MQ=60)

                                          TAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGG                                                     <  SRR4302532.1364579/94‑1 (MQ=60)

                                          TAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGG                                                     <  SRR4302532.1764802/94‑1 (MQ=60)

                                           AAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGC                                                    <  SRR4302532.977777/94‑1 (MQ=60)

                                           AAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGC                                                    >  SRR4302532.3694996/1‑94 (MQ=60)

                                            AAGATTTTCACTGTTACTTCCGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCA                                                   <  SRR4302532.4196761/94‑1 (MQ=60)

                                            AAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCA                                                   <  SRR4302532.2858829/94‑1 (MQ=60)

                                            AAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCA                                                   <  SRR4302532.5031069/94‑1 (MQ=60)

                                             AGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCAC                                                  >  SRR4302532.2107675/1‑94 (MQ=60)

                                               ATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGT                                                >  SRR4302532.5529215/1‑94 (MQ=60)

                                                  TTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTT                                             >  SRR4302532.6251233/1‑94 (MQ=60)

                                                  TTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTT                                             >  SRR4302532.5146396/1‑94 (MQ=60)

                                                  TTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTT                                             <  SRR4302532.1138916/94‑1 (MQ=60)

                                                  TTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGCGGCAGGTTTT                                             >  SRR4302532.1347596/1‑94 (MQ=60)

                                                   TCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTT                                            >  SRR4302532.3300097/1‑94 (MQ=60)

                                                    CACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTA                                           >  SRR4302532.396146/1‑94 (MQ=60)

                                                     ACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCCCGTTTTTAT                                          >  SRR4302532.2640256/1‑94 (MQ=60)

                                                     ACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAA                                          >  SRR4302532.881859/1‑94 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302532.2577241/1‑94 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         <  SRR4302532.2167554/94‑1 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302532.986011/1‑94 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         <  SRR4302532.3502059/94‑1 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         <  SRR4302532.3583577/94‑1 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAACCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302532.2732820/1‑94 (MQ=60)

                                                      CTGTTACTTCAGTGATATAGCACAATGCACCTCCGTT‑GCCGATGATGATGTGACGAATGTCCCGGCCCGCCGCTGGGCGTGGCACGatctttat                                         >  SRR4302532.2577242/1‑86 (MQ=60)

                                                       TGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATG                                        >  SRR4302532.4610077/1‑94 (MQ=60)

                                                        GTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGC                                       >  SRR4302532.66726/1‑94 (MQ=60)

                                                         TTACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCG                                      >  SRR4302532.3730712/1‑94 (MQ=60)

                                                          TACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGT                                     >  SRR4302532.6317543/1‑94 (MQ=60)

                                                          TACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGT                                     <  SRR4302532.3201427/94‑1 (MQ=60)

                                                           ACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTG                                    >  SRR4302532.900882/1‑94 (MQ=60)

                                                           ACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTG                                    >  SRR4302532.1220630/1‑94 (MQ=60)

                                                           ACTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTG                                    >  SRR4302532.2458403/1‑94 (MQ=60)

                                                            CTTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGT                                   <  SRR4302532.6211844/94‑1 (MQ=60)

                                                             TTCAGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTG                                  >  SRR4302532.4264652/1‑94 (MQ=60)

                                                                AGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGTATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               <  SRR4302532.2533907/94‑1 (MQ=60)

                                                                AGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTGCGGGCCAGCCGCGCGGCGTGGCACGatttttatttgtatgacc                               >  SRR4302532.5584857/1‑76 (MQ=60)

                                                                AGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               <  SRR4302532.1420305/94‑1 (MQ=60)

                                                                AGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               >  SRR4302532.5842882/1‑94 (MQ=60)

                                                                AGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               >  SRR4302532.2445257/1‑94 (MQ=60)

                                                                AGTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               <  SRR4302532.1201129/94‑1 (MQ=60)

                                                                 GTGATATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGG                              >  SRR4302532.4434392/1‑94 (MQ=60)

                                                                     TATAGCACAATGCTCCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCC                          <  SRR4302532.4067969/94‑1 (MQ=60)

                                                                     TATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCC                          >  SRR4302532.2980389/1‑94 (MQ=60)

                                                                     TATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCC                          >  SRR4302532.6218755/1‑94 (MQ=60)

                                                                     TATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCC                          <  SRR4302532.4067968/94‑1 (MQ=60)

                                                                     TATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCC                          >  SRR4302532.4495588/1‑94 (MQ=60)

                                                                      ATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCT                         >  SRR4302532.5379013/1‑94 (MQ=60)

                                                                      ATAGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCA                         >  SRR4302532.2133605/1‑94 (MQ=60)

                                                                        AGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATC                       >  SRR4302532.3372519/1‑94 (MQ=60)

                                                                        AGCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATC                       <  SRR4302532.3371632/94‑1 (MQ=60)

                                                                         GCACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCA                      <  SRR4302532.5800520/94‑1 (MQ=60)

                                                                          CACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAG                     >  SRR4302532.5322766/1‑94 (MQ=60)

                                                                          CACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAG                     >  SRR4302532.2656530/1‑94 (MQ=60)

                                                                           ACAATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGC                    <  SRR4302532.2033547/94‑1 (MQ=60)

                                                                              ATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAA                 >  SRR4302532.4655127/1‑94 (MQ=60)

                                                                              ATGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGCGCCATCAGCCAA                 >  SRR4302532.4726043/1‑94 (MQ=60)

                                                                               TGCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAAT                <  SRR4302532.3918899/94‑1 (MQ=60)

                                                                                GCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATA               >  SRR4302532.842102/1‑94 (MQ=60)

                                                                                GCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATA               >  SRR4302532.3151100/1‑94 (MQ=60)

                                                                                GCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATA               >  SRR4302532.1632097/1‑94 (MQ=60)

                                                                                GCACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATA               >  SRR4302532.973973/1‑94 (MQ=60)

                                                                                 CACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATAC              <  SRR4302532.4980345/94‑1 (MQ=60)

                                                                                 CACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATAC              <  SRR4302532.5618773/94‑1 (MQ=60)

                                                                                  ACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACT             >  SRR4302532.4858259/1‑94 (MQ=60)

                                                                                  ACCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAACACT             >  SRR4302532.309628/1‑94 (MQ=60)

                                                                                   CCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTG            >  SRR4302532.4850758/1‑94 (MQ=60)

                                                                                   CCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTG            >  SRR4302532.4726108/1‑94 (MQ=60)

                                                                                   CCTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTG            >  SRR4302532.4683366/1‑94 (MQ=60)

                                                                                    CTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGC           <  SRR4302532.6238170/94‑1 (MQ=60)

                                                                                    CTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGC           >  SRR4302532.1826108/1‑94 (MQ=60)

                                                                                    CTTCGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGC           >  SRR4302532.3015286/1‑94 (MQ=60)

                                                                                       CGTT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTC        >  SRR4302532.3200996/1‑94 (MQ=60)

                                                                                         gtggcCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCC       <  SRR4302532.2164993/89‑1 (MQ=60)

                                                                                         TT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCA      <  SRR4302532.3443746/94‑1 (MQ=60)

                                                                                         TT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCA      <  SRR4302532.6254406/94‑1 (MQ=60)

                                                                                         TT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCA      >  SRR4302532.5469675/1‑94 (MQ=60)

                                                                                         TT‑GCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTCCCATCAGCCAATACTGCTTCCA      >  SRR4302532.5469673/1‑94 (MQ=60)

                                                                                           tgcCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAG     >  SRR4302532.4621583/4‑94 (MQ=60)

                                                                                              CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACC  <  SRR4302532.831756/94‑1 (MQ=60)

CCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTT‑G‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACC  >  NC_000913/2896727‑2896912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`