Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,896,819	+C	coding (622/657 nt)	ygcU ←	putative oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,896,818	1	.	C	99.6%	855.8 / ‑3.8	231	coding (623/657 nt)	ygcU	putative oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (129/101); total (129/102)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 251 total aligned reads displayed.

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑C‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTT  >  NC_000913/2896726‑2896905

tCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTg                                                                                           >  1:2194838/1‑93 (MQ=255)

 ccAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑c                                                                                         <  2:5427869/93‑1 (MQ=255)

 ccAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑c                                                                                         <  2:4697089/93‑1 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                      >  2:2234603/1‑90 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                      <  1:3937997/93‑4 (MQ=255)

    ggATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccga                                                                                      >  1:4696897/1‑90 (MQ=255)

     gATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑ccgat                                                                                     >  2:3101810/1‑89 (MQ=255)

       tataGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatga                                                                                   <  2:3743024/93‑1 (MQ=255)

       tataGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatga                                                                                   <  2:6047699/93‑1 (MQ=255)

       tataGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatga                                                                                   >  1:2355687/1‑93 (MQ=255)

        ataGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatgat                                                                                  <  2:5098220/93‑1 (MQ=255)

          aGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatgatga                                                                                <  2:705140/93‑1 (MQ=255)

          aGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatgatga                                                                                <  1:2387546/93‑1 (MQ=255)

           gCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatgatgat                                                                               >  1:4839747/1‑93 (MQ=255)

           gCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑gatgatgat                                                                               >  2:4398277/1‑93 (MQ=255)

            ccGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑Gatgatgatg                                                                              >  2:4817492/1‑93 (MQ=255)

             cGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGAtgt                                                                             <  2:2282383/93‑1 (MQ=255)

             cGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGAtgt                                                                             >  1:2495088/1‑93 (MQ=255)

               tAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGa                                                                           <  1:4613572/93‑1 (MQ=255)

                   agagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGaa                                                                       <  1:635459/93‑1 (MQ=255)

                   agagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGaa                                                                       >  2:2014456/1‑93 (MQ=255)

                    gagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAAt                                                                      >  2:3409142/1‑93 (MQ=255)

                    gagGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAAt                                                                      >  2:9559/1‑93 (MQ=255)

                     agGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATg                                                                     <  1:5656528/93‑1 (MQ=255)

                     agGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATg                                                                     <  2:6273445/93‑1 (MQ=255)

                     agGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATg                                                                     >  1:2595498/1‑93 (MQ=255)

                      ggTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGt                                                                    >  1:1493674/1‑93 (MQ=255)

                        ttgttTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCACTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTcc                                                                  >  2:3565072/1‑93 (MQ=255)

                         tgttTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCg                                                                 <  1:6237087/93‑1 (MQ=255)

                         tgttTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCg                                                                 <  1:1433779/93‑1 (MQ=255)

                           ttttCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCggg                                                               <  1:2315860/93‑1 (MQ=255)

                              tCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCa                                                            >  1:208508/1‑93 (MQ=255)

                               ccGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAg                                                           <  1:5535026/93‑1 (MQ=255)

                                cGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGc                                                          >  2:3186392/1‑93 (MQ=255)

                                cGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGc                                                          <  1:5219110/93‑1 (MQ=255)

                                cGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGc                                                          <  1:2014843/93‑1 (MQ=255)

                                 ggggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGcc                                                         >  2:3921154/1‑93 (MQ=255)

                                  gggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCcg                                                        <  1:5335284/93‑1 (MQ=255)

                                  gggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCcg                                                        >  2:5519317/1‑93 (MQ=255)

                                   ggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCcgc                                                       <  2:1638404/93‑1 (MQ=255)

                                    gTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCcgcg                                                      >  1:3657025/1‑93 (MQ=255)

                                          ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGt                                                >  1:6297197/1‑93 (MQ=255)

                                           tAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTg                                               >  1:3622766/1‑93 (MQ=255)

                                           tAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTg                                               <  2:6081575/93‑1 (MQ=255)

                                             aaGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGc                                             >  2:1368897/1‑93 (MQ=255)

                                              aGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCa                                            >  1:1760355/1‑93 (MQ=255)

                                              aGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCa                                            <  2:3265620/93‑1 (MQ=255)

                                                  tttCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCATCCGCGCGGCGAGGCACGtt                                        <  1:6484366/93‑1 (MQ=255)

                                                    tCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGtttt                                      <  2:3301394/93‑1 (MQ=255)

                                                    tCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGtttt                                      <  1:4495577/93‑1 (MQ=255)

                                                      aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTa                                    >  2:4129525/1‑93 (MQ=255)

                                                      aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTa                                    >  2:4649236/1‑93 (MQ=255)

                                                      aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTa                                    >  1:1659232/1‑93 (MQ=255)

                                                      aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTa                                    >  1:2699085/1‑93 (MQ=255)

                                                      aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTa                                    >  2:566653/1‑93 (MQ=255)

                                                       cTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTaa                                   >  2:76008/1‑93 (MQ=255)

                                                       cTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTaa                                   >  2:2984827/1‑93 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAt                                  >  2:5199693/1‑93 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAt                                  >  1:1133548/1‑93 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAt                                  >  1:5832385/1‑93 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAt                                  <  1:6513569/93‑1 (MQ=255)

                                                        tGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAt                                  >  2:1223327/1‑93 (MQ=255)

                                                          ttACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTTAttc                                >  2:4513747/1‑91 (MQ=255)

                                                          ttACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGc                                <  2:1884460/93‑1 (MQ=255)

                                                           tACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCg                               <  2:428768/93‑1 (MQ=255)

                                                           tACTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCg                               >  1:4373484/1‑93 (MQ=255)

                                                           tACTTCAGTGATATAGCACAATGCACCTTCGTTG‑CAGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCg                               <  2:5125641/93‑1 (MQ=255)

                                                            aCTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgt                              <  1:1846988/93‑1 (MQ=255)

                                                             cTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgtg                             >  2:1096591/1‑93 (MQ=255)

                                                             cTTCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCgtg                             >  1:6085196/1‑93 (MQ=255)

                                                               tCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGtgtg                           <  1:781446/93‑1 (MQ=255)

                                                               tCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGtgtg                           >  1:5558611/1‑93 (MQ=255)

                                                               tCAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGtgtg                           >  1:1725936/1‑93 (MQ=255)

                                                                cAGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGa                          >  1:4022642/1‑93 (MQ=255)

                                                                 aGTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGAc                         >  1:3117671/1‑93 (MQ=255)

                                                                  gTGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACg                        >  1:3199014/1‑93 (MQ=255)

                                                                   tGATATAGCACAATGCGCCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACgg                       <  1:3816140/93‑1 (MQ=255)

                                                                   tGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACgg                       >  2:1902791/1‑93 (MQ=255)

                                                                   tGATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACgg                       >  2:5032458/1‑93 (MQ=255)

                                                                    gATATAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGt                      <  1:5939274/93‑1 (MQ=255)

                                                                     atatAGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTg                     >  2:4765885/1‑93 (MQ=255)

                                                                      tataGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGc                    >  2:1550981/1‑93 (MQ=255)

                                                                       ataGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGcc                   <  2:3343468/93‑1 (MQ=255)

                                                                       ataGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGcc                   >  1:2123577/1‑93 (MQ=255)

                                                                        taGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCa                  >  1:1040739/1‑93 (MQ=255)

                                                                        taGCACAATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCa                  <  2:2466540/93‑1 (MQ=255)

                                                                            acaATGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAg              >  1:4076590/1‑93 (MQ=255)

                                                                                tGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCaa          >  2:6502159/1‑93 (MQ=255)

                                                                                tGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCaa          <  2:1009660/93‑1 (MQ=255)

                                                                                tGCACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCaa          >  2:2661207/1‑93 (MQ=255)

                                                                                  cACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATa        <  2:45352/93‑1 (MQ=255)

                                                                                  cACCTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATa        >  1:1951907/1‑93 (MQ=255)

                                                                                    ccTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACt      >  1:6387867/1‑93 (MQ=255)

                                                                                    ccTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACt      <  2:2400246/93‑1 (MQ=255)

                                                                                    ccTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACt      >  2:4323859/1‑93 (MQ=255)

                                                                                     cTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTg     <  1:5064589/93‑1 (MQ=255)

                                                                                     cTTCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTg     >  2:203292/1‑93 (MQ=255)

                                                                                       tCGTTGCC‑GNTGATGATGTGACGAATGTCCGGGCCAGCCGCNCGGNGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCt   >  2:5415297/1‑93 (MQ=255)

                                                                                       tCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCt   <  2:2828015/93‑1 (MQ=255)

                                                                                       tCGTTGCC‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCt   <  1:3006315/93‑1 (MQ=255)

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑C‑GATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTT  >  NC_000913/2896726‑2896905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACC  >  NC_000913/2896726‑2896912

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑C                                                                                               >  SRR4302547.2593751/1‑94 (MQ=60)

 CCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑CC                                                                                              >  SRR4302547.5510209/1‑94 (MQ=60)

  CAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAATATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑Ccg                                                                                             <  SRR4302547.5694345/94‑3 (MQ=60)

  CAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑Ccg                                                                                             >  SRR4302547.5964777/1‑92 (MQ=60)

   AGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑Ccga                                                                                            >  SRR4302547.4794187/1‑91 (MQ=60)

    GGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGAT                                                                                            <  SRR4302547.4018067/94‑1 (MQ=60)

     GATATAGCCGTAGCAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATG                                                                                           >  SRR4302547.2029088/1‑94 (MQ=60)

      ATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGA                                                                                          >  SRR4302547.2403423/1‑94 (MQ=60)

       TATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGAT                                                                                         <  SRR4302547.406492/94‑1 (MQ=60)

       TATAGCCGTAGAAGAGCTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGAT                                                                                         >  SRR4302547.6056151/1‑94 (MQ=60)

        ATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACACTGCACCTTCGTTGCCGATGATG                                                                                        >  SRR4302547.3706191/1‑94 (MQ=60)

        ATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATG                                                                                        >  SRR4302547.315025/1‑94 (MQ=60)

        ATAGCCGTAGAAGAGGTTGTTTTCCGGGGTAAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATG                                                                                        <  SRR4302547.4473623/94‑1 (MQ=60)

          AGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGAT                                                                                      >  SRR4302547.4940058/1‑94 (MQ=60)

          AGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGAT                                                                                      <  SRR4302547.239220/94‑1 (MQ=60)

          AGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGAT                                                                                      <  SRR4302547.2435916/94‑1 (MQ=60)

            CCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGT                                                                                    >  SRR4302547.2545498/1‑94 (MQ=60)

             CGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTG                                                                                   >  SRR4302547.2082122/1‑94 (MQ=60)

             CGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTG                                                                                   <  SRR4302547.1799210/94‑1 (MQ=60)

               TAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGAC                                                                                 <  SRR4302547.4709077/94‑1 (MQ=60)

                AGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACG                                                                                >  SRR4302547.2706555/1‑94 (MQ=60)

                  AAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAA                                                                              >  SRR4302547.2015641/1‑94 (MQ=60)

                   AGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAAT                                                                             <  SRR4302547.647191/94‑1 (MQ=60)

                    GAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATG                                                                            >  SRR4302547.2647831/1‑94 (MQ=60)

                    GAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATG                                                                            <  SRR4302547.4431273/94‑1 (MQ=60)

                    GAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATG                                                                            <  SRR4302547.6115124/94‑1 (MQ=60)

                     AGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGT                                                                           >  SRR4302547.1523472/1‑94 (MQ=60)

                     AGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGT                                                                           <  SRR4302547.5773295/94‑1 (MQ=60)

                         TGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGG                                                                       <  SRR4302547.1462444/94‑1 (MQ=60)

                         TGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGG                                                                       <  SRR4302547.6366664/94‑1 (MQ=60)

                          GTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGG                                                                      <  SRR4302547.2130671/94‑1 (MQ=60)

                           TTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGC                                                                     <  SRR4302547.2362807/94‑1 (MQ=60)

                             TTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCA                                                                   >  SRR4302547.212438/1‑94 (MQ=60)

                               CCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGC                                                                 <  SRR4302547.5649259/94‑1 (MQ=60)

                                CGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCC                                                                <  SRR4302547.2054930/94‑1 (MQ=60)

                                CGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCC                                                                <  SRR4302547.5326528/94‑1 (MQ=60)

                                 GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCG                                                               <  SRR4302547.5332902/94‑1 (MQ=60)

                                 GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCG                                                               <  SRR4302547.24922/94‑1 (MQ=60)

                                  GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302547.3281835/1‑94 (MQ=60)

                                  GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              <  SRR4302547.5445222/94‑1 (MQ=60)

                                  GGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGC                                                              >  SRR4302547.637564/1‑94 (MQ=60)

                                   GGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCG                                                             >  SRR4302547.3731636/1‑94 (MQ=60)

                                     TGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCG                                                           >  SRR4302547.5661891/1‑94 (MQ=60)

                                     TGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCG                                                           <  SRR4302547.2858784/94‑1 (MQ=60)

                                     TGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCG                                                           >  SRR4302547.6648547/1‑94 (MQ=60)

                                       AATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGC                                                         >  SRR4302547.3864113/1‑94 (MQ=60)

                                       AATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTAGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGC                                                         <  SRR4302547.2229324/94‑1 (MQ=60)

                                        ATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCG                                                        >  SRR4302547.4749203/1‑94 (MQ=60)

                                         TTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGT                                                       >  SRR4302547.3553652/1‑94 (MQ=60)

                                         TTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGT                                                       >  SRR4302547.6428096/1‑94 (MQ=60)

                                          TTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTG                                                      >  SRR4302547.3696672/1‑94 (MQ=60)

                                          TTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTG                                                      >  SRR4302547.6276195/1‑94 (MQ=60)

                                           TAAAGATTTTCACTGTTACTCCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGG                                                     <  SRR4302547.5171757/94‑1 (MQ=60)

                                             AAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCA                                                   >  SRR4302547.3968900/1‑94 (MQ=60)

                                             AAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCA                                                   >  SRR4302547.1795252/1‑94 (MQ=60)

                                                 TTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTT                                               <  SRR4302547.1536674/94‑1 (MQ=60)

                                                  TTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCATCCGCGCGGCGAGGCACGTTT                                              <  SRR4302547.6619363/94‑1 (MQ=60)

                                                   TTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTT                                             <  SRR4302547.5551771/94‑1 (MQ=60)

                                                    TCACTGTTACTTCAGTGATATAGTACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTT                                            <  SRR4302547.5887097/94‑1 (MQ=60)

                                                    TCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTT                                            >  SRR4302547.1936471/1‑94 (MQ=60)

                                                    TCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTT                                            <  SRR4302547.4588428/94‑1 (MQ=60)

                                                    TCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTT                                            >  SRR4302547.6771368/1‑94 (MQ=60)

                                                    TCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTT                                            >  SRR4302547.5212194/1‑94 (MQ=60)

                                                     CACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTA                                           <  SRR4302547.1513549/94‑1 (MQ=60)

                                                     CACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTA                                           >  SRR4302547.1691958/1‑94 (MQ=60)

                                                     CACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTA                                           >  SRR4302547.2753267/1‑94 (MQ=60)

                                                     CACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTA                                           >  SRR4302547.2063029/1‑94 (MQ=60)

                                                      ACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAA                                          >  SRR4302547.2243353/1‑94 (MQ=60)

                                                      ACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAA                                          >  SRR4302547.4934015/1‑94 (MQ=60)

                                                      ACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAA                                          >  SRR4302547.1395634/1‑94 (MQ=60)

                                                       CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302547.6327290/1‑94 (MQ=60)

                                                       CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302547.5953067/1‑94 (MQ=60)

                                                       CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302547.1156439/1‑94 (MQ=60)

                                                       CTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAAT                                         >  SRR4302547.679487/1‑94 (MQ=60)

                                                        TGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGGACGTTTTTAATG                                        >  SRR4302547.4604507/1‑94 (MQ=60)

                                                        TGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATG                                        <  SRR4302547.1125429/94‑1 (MQ=60)

                                                        TGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATG                                        <  SRR4302547.6649163/94‑1 (MQ=60)

                                                        TGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATG                                        >  SRR4302547.4110349/1‑94 (MQ=60)

                                                          TTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCG                                      >  SRR4302547.4463360/1‑94 (MQ=60)

                                                          TTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCG                                      >  SRR4302547.4414961/1‑94 (MQ=60)

                                                          TTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTCTTAATGCG                                      <  SRR4302547.6444435/94‑1 (MQ=60)

                                                           TACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGT                                     >  SRR4302547.6474232/1‑94 (MQ=60)

                                                            ACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTG                                    >  SRR4302547.6552971/1‑94 (MQ=60)

                                                            ACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTG                                    >  SRR4302547.6211439/1‑94 (MQ=60)

                                                            ACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTG                                    <  SRR4302547.1883503/94‑1 (MQ=60)

                                                             CTTCAGTGATATAGCACAAAGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTCATGCGTGT                                   >  SRR4302547.2727252/1‑94 (MQ=60)

                                                              TTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTG                                  <  SRR4302547.5747500/94‑1 (MQ=60)

                                                              TTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTG                                  >  SRR4302547.1759850/1‑94 (MQ=60)

                                                              TTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTG                                  >  SRR4302547.5673268/1‑94 (MQ=60)

                                                              TTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTG                                  >  SRR4302547.2461997/1‑94 (MQ=60)

                                                              TTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTG                                  >  SRR4302547.4087064/1‑94 (MQ=60)

                                                               TCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGA                                 <  SRR4302547.796221/94‑1 (MQ=60)

                                                               TCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGA                                 >  SRR4302547.3184151/1‑94 (MQ=60)

                                                               TCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGA                                 >  SRR4302547.4104379/1‑94 (MQ=60)

                                                                CAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGAC                                >  SRR4302547.3179469/1‑94 (MQ=60)

                                                                 AGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               >  SRR4302547.3262796/1‑94 (MQ=60)

                                                                 AGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               <  SRR4302547.1768538/94‑1 (MQ=60)

                                                                 AGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACG                               >  SRR4302547.4936142/1‑94 (MQ=60)

                                                                   TGATATAGCACAATGCGCCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGT                             <  SRR4302547.3893895/94‑1 (MQ=60)

                                                                    GATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTG                            >  SRR4302547.1773740/1‑94 (MQ=60)

                                                                    GATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTG                            <  SRR4302547.6062427/94‑1 (MQ=60)

                                                                      TATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCC                          >  SRR4302547.2165902/1‑94 (MQ=60)

                                                                       ATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGTCAGCCCCGCGGCGAGGCACGTTTTTAATGCGTGTGACGCTGCCA                         >  SRR4302547.1594118/1‑94 (MQ=60)

                                                                       ATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCA                         >  SRR4302547.903994/1‑94 (MQ=60)

                                                                       ATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCA                         >  SRR4302547.1061258/1‑94 (MQ=60)

                                                                       ATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCA                         >  SRR4302547.193426/1‑94 (MQ=60)

                                                                        TAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCAT                        <  SRR4302547.1426400/94‑1 (MQ=60)

                                                                         AGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATC                       <  SRR4302547.5048712/94‑1 (MQ=60)

                                                                          GCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCA                      <  SRR4302547.3839442/94‑1 (MQ=60)

                                                                           CACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAG                     >  SRR4302547.4159425/1‑94 (MQ=60)

                                                                           CACAAAGCACCTTCGTTGCCGGTGATGATGTGACGAATGGCCGGGCCAGCCGCCCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAG                     <  SRR4302547.2221349/94‑1 (MQ=60)

                                                                            ACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGC                    >  SRR4302547.3106809/1‑94 (MQ=60)

                                                                             CAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCC                   >  SRR4302547.2412204/1‑94 (MQ=60)

                                                                             CAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCC                   >  SRR4302547.1757246/1‑94 (MQ=60)

                                                                              AATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCA                  >  SRR4302547.459288/1‑94 (MQ=60)

                                                                               ATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAA                 <  SRR4302547.65347/94‑1 (MQ=60)

                                                                                 GCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATA               >  SRR4302547.1990624/1‑94 (MQ=60)

                                                                                   ACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACT             >  SRR4302547.2389484/1‑94 (MQ=60)

                                                                                   ACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACT             >  SRR4302547.6521149/1‑94 (MQ=60)

                                                                                     CTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGC           <  SRR4302547.5169102/94‑1 (MQ=60)

                                                                                       TCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTT         <  SRR4302547.3066101/94‑1 (MQ=60)

                                                                                       TCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTT         >  SRR4302547.6500093/1‑94 (MQ=60)

                                                                                        CGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTC        >  SRR4302547.1728292/1‑94 (MQ=60)

                                                                                        CGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTC        >  SRR4302547.1570422/1‑94 (MQ=60)

                                                                                         GTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCC       <  SRR4302547.4191836/94‑1 (MQ=60)

                                                                                          TTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCA      <  SRR4302547.1396230/94‑1 (MQ=60)

                                                                                           tgcCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAG     <  SRR4302547.4657531/91‑1 (MQ=60)

                                                                                            gcCGATGATGTTGTGACGAATGCCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGA    <  SRR4302547.4778974/92‑1 (MQ=60)

                                                                                            gcCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGA    >  SRR4302547.3766179/3‑94 (MQ=60)

                                                                                            gcCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGA    >  SRR4302547.1769057/3‑94 (MQ=60)

                                                                                            gcCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGA    <  SRR4302547.5610957/92‑1 (MQ=60)

                                                                                            C‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCGATCAGCCAATACTGCTTCCAGAC   >  SRR4302547.3131266/1‑94 (MQ=60)

                                                                                              CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACC  >  SRR4302547.333249/1‑94 (MQ=60)

TCCAGGATATAGCCGTAGAAGAGGTTGTTTTCCGGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTG‑CGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACC  >  NC_000913/2896726‑2896912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`