Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,930,677	+G	intergenic (+175/‑264)	kup → / → rbsD	low affinity potassium transport system/RbsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,930,676	1	.	G	99.6%	811.9 / ‑5.8	227	intergenic (+174/‑265)	kup/rbsD	low affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand): ref base . (0/1); major base G (114/111); minor base T (1/0); total (115/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 257 total aligned reads displayed.

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA  >  NC_000913/3930584‑3930763

gTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTc                                                                                           <  1:1483043/93‑1 (MQ=255)

gTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTc                                                                                           >  1:5620353/1‑93 (MQ=255)

gTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGAATGAGTCGTTGATCCTc                                                                                           <  2:4234248/93‑1 (MQ=255)

 tAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑g                                                                                         <  2:466162/93‑1 (MQ=255)

 tAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑g                                                                                         <  2:766370/93‑1 (MQ=255)

 tAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑g                                                                                         <  1:3150683/93‑1 (MQ=255)

   gaagaaGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggc                                                                                       >  2:6069826/1‑91 (MQ=255)

   gaagaaGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggc                                                                                       >  2:3472178/1‑91 (MQ=255)

   gaagaaGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggc                                                                                       >  2:278395/1‑91 (MQ=255)

    aagaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggca                                                                                      >  2:717776/1‑90 (MQ=255)

    aagaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggca                                                                                      >  1:258984/1‑90 (MQ=255)

     agaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggcaa                                                                                     >  1:6057921/1‑89 (MQ=255)

     agaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑ggcaa                                                                                     >  1:5513432/1‑89 (MQ=255)

         gTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGc                                                                                 >  1:1388663/1‑93 (MQ=255)

           tttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGccc                                                                               >  1:1119969/1‑93 (MQ=255)

             tCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGt                                                                             >  2:4077875/1‑93 (MQ=255)

              ccACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTg                                                                            <  2:886240/93‑1 (MQ=255)

               cACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTgg                                                                           >  2:3588794/1‑93 (MQ=255)

               cACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTgg                                                                           >  2:395139/1‑93 (MQ=255)

                cccGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGt                                                                          <  2:710446/92‑1 (MQ=255)

                aCCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGt                                                                          >  1:3095929/1‑93 (MQ=255)

                 ccGGTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTa                                                                         <  2:2156677/93‑1 (MQ=255)

                 ccGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCTGCAAA‑CGCCCGTggct                                                                         >  1:2348632/1‑91 (MQ=255)

                 ccGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTa                                                                         >  2:5011183/1‑93 (MQ=255)

                    cTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTAttt                                                                      >  2:105846/1‑93 (MQ=255)

                     tGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTg                                                                     <  2:3643373/93‑1 (MQ=255)

                      gCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGc                                                                    <  2:3272692/93‑1 (MQ=255)

                       cGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCg                                                                   <  2:4465348/93‑1 (MQ=255)

                        ggTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGt                                                                  <  1:1006440/93‑1 (MQ=255)

                         gtgtGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTc                                                                 <  1:4596389/90‑1 (MQ=255)

                         gTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTc                                                                 <  2:2417911/93‑1 (MQ=255)

                          tCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCATGGTATTTGCGTCt                                                                >  2:5059361/1‑93 (MQ=255)

                          gcTGGAAGGATTAAGTTGCCGGATGATTGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCt                                                                <  1:5335819/92‑1 (MQ=255)

                           cTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTg                                                               <  1:294582/93‑1 (MQ=255)

                            tGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTgc                                                              <  1:6085539/93‑1 (MQ=255)

                              gAAGGATTAAGTTGCCTGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTgcgc                                                            <  2:5175304/93‑1 (MQ=255)

                              gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTgcgc                                                            >  1:4879685/1‑93 (MQ=255)

                              gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTgcgc                                                            >  2:3368522/1‑93 (MQ=255)

                                   aTTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCaa                                                       <  1:5044995/93‑1 (MQ=255)

                                   aTTAAGGTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCaa                                                       <  1:1230621/93‑1 (MQ=255)

                                     tAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGc                                                     <  2:5354330/93‑1 (MQ=255)

                                       aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTg                                                   <  2:2832401/93‑1 (MQ=255)

                                       aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTg                                                   <  2:571306/93‑1 (MQ=255)

                                       aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTg                                                   >  2:1516515/1‑93 (MQ=255)

                                        gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                                  <  2:2613363/93‑1 (MQ=255)

                                        gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                                  <  1:1026651/93‑1 (MQ=255)

                                           gCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACt                                               <  1:70735/93‑1 (MQ=255)

                                            ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACtt                                              >  2:1204140/1‑93 (MQ=255)

                                            ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACtt                                              <  1:1974461/93‑1 (MQ=255)

                                             cGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtg                                             >  1:2742084/1‑93 (MQ=255)

                                              ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                            >  1:3778375/1‑93 (MQ=255)

                                              ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                            >  1:2261050/1‑93 (MQ=255)

                                              cgatgatGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                            <  1:511839/92‑1 (MQ=255)

                                               gatgatGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgct                                           <  1:1298248/93‑1 (MQ=255)

                                                atgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTTTGCGCGGCAAGCTGTACTtgctg                                          >  1:3515323/1‑93 (MQ=255)

                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                         >  2:6007429/1‑93 (MQ=255)

                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                         >  1:2701681/1‑93 (MQ=255)

                                                    tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCCAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                      >  1:1587917/1‑93 (MQ=255)

                                                    tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                      >  2:1883791/1‑93 (MQ=255)

                                                    tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                      >  1:4366129/1‑93 (MQ=255)

                                                        aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                                  <  2:1336969/93‑1 (MQ=255)

                                                        aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                                  >  2:1305530/1‑93 (MQ=255)

                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                 >  2:4730899/1‑93 (MQ=255)

                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                 <  2:4583298/93‑1 (MQ=255)

                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                 <  2:3571410/93‑1 (MQ=255)

                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                                >  2:5860461/1‑93 (MQ=255)

                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                                >  2:173065/1‑93 (MQ=255)

                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                                <  1:4249986/93‑1 (MQ=255)

                                                           ctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTaa                               >  2:193929/1‑93 (MQ=255)

                                                           ctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTaa                               >  1:3348518/1‑93 (MQ=255)

                                                            tcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAt                              <  1:4017112/93‑1 (MQ=255)

                                                            tcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAt                              <  1:964290/93‑1 (MQ=255)

                                                            tcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAt                              <  2:2562552/93‑1 (MQ=255)

                                                             cctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATg                             >  2:5181606/1‑93 (MQ=255)

                                                             cctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATg                             <  2:4849448/93‑1 (MQ=255)

                                                              cttcttTAAGTCGCATGTGTCGTTGATCCTC‑GGAAATCGCCCGAGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAGCGTattac                            <  1:5493840/93‑5 (MQ=255)

                                                               ttcttTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg                           >  2:3059731/1‑93 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          <  2:1157519/93‑1 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          <  2:4517590/93‑1 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          <  1:3179334/93‑1 (MQ=255)

                                                                 cttTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgcg                         >  2:290831/1‑92 (MQ=255)

                                                                  tttATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTg                        >  1:5839353/1‑93 (MQ=255)

                                                                   ttATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGc                       <  1:5427265/93‑1 (MQ=255)

                                                                    tATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCg                      <  1:1834576/93‑1 (MQ=255)

                                                                    tATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCg                      >  2:4438538/1‑93 (MQ=255)

                                                                     ttGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGt                     <  2:3906754/92‑1 (MQ=255)

                                                                       gTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGc                   >  2:948399/1‑93 (MQ=255)

                                                                       gTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTCGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGc                   <  1:4118662/93‑1 (MQ=255)

                                                                          gCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAcc                >  2:4186537/1‑93 (MQ=255)

                                                                            aTGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAg              <  2:4327399/93‑1 (MQ=255)

                                                                             tGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa             >  2:1681635/1‑93 (MQ=255)

                                                                                gTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCa          <  2:3253122/93‑1 (MQ=255)

                                                                                gTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCa          >  1:3115026/1‑93 (MQ=255)

                                                                                 tCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCaa         <  2:2105285/93‑1 (MQ=255)

                                                                                   gTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt       <  1:3673081/93‑1 (MQ=255)

                                                                                    ttGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAttt      >  2:3404202/1‑93 (MQ=255)

                                                                                       aTCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaa   <  1:4860535/93‑1 (MQ=255)

                                                                                        tCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaaa  <  1:5089625/93‑1 (MQ=255)

                                                                                        tCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaaa  >  1:3543562/1‑93 (MQ=255)

                                                                                        tCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaaa  <  2:3990127/93‑1 (MQ=255)

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA  >  NC_000913/3930584‑3930763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC  >  NC_000913/3930584‑3930769

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑G                                                                                               <  SRR4302532.1533047/94‑1 (MQ=60)

CTAGAAGAGGGTTTCCACCGCTTCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGGCGTTGATCCTC‑G                                                                                               <  SRR4302532.4229732/94‑1 (MQ=60)

 TAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GG                                                                                              <  SRR4302532.3255219/94‑1 (MQ=60)

   GAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggca                                                                                            >  SRR4302532.267969/1‑91 (MQ=60)

   GAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggca                                                                                            >  SRR4302532.4292728/1‑91 (MQ=60)

   GAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggca                                                                                            >  SRR4302532.267970/1‑91 (MQ=60)

    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302532.5704760/1‑94 (MQ=60)

    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302532.6267089/1‑94 (MQ=60)

     AGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA                                                                                           >  SRR4302532.1038913/1‑94 (MQ=60)

     AGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA                                                                                           >  SRR4302532.443065/1‑94 (MQ=60)

      GAAGTTTTCCACCGCTGCTGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑C                                                                                         <  SRR4302532.207017/94‑1 (MQ=60)

      GAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑C                                                                                         <  SRR4302532.207015/94‑1 (MQ=60)

        AGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGC                                                                                       >  SRR4302532.1435209/1‑94 (MQ=60)

        AGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGC                                                                                       >  SRR4302532.955528/1‑94 (MQ=60)

          TTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCC                                                                                     <  SRR4302532.505306/94‑1 (MQ=60)

          TTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCC                                                                                     >  SRR4302532.1157368/1‑94 (MQ=60)

            TTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGT                                                                                   <  SRR4302532.3660463/94‑1 (MQ=60)

               CACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGT                                                                                >  SRR4302532.3198578/1‑94 (MQ=60)

               CACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGT                                                                                >  SRR4302532.3700137/1‑94 (MQ=60)

                ACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCTGCAAA‑CGCCCGTGGct                                                                               >  SRR4302532.2426976/1‑92 (MQ=60)

                ACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTA                                                                               >  SRR4302532.3221098/1‑94 (MQ=60)

                ACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTA                                                                               >  SRR4302532.3167743/1‑94 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATT                                                                             <  SRR4302532.2985774/94‑1 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CCCCCGTGGTATT                                                                             >  SRR4302532.4285734/1‑94 (MQ=60)

                       CGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGT                                                                        <  SRR4302532.5718643/94‑1 (MQ=60)

                        GGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTC                                                                       >  SRR4302532.5552521/1‑94 (MQ=60)

                        GGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTC                                                                       <  SRR4302532.1040684/94‑1 (MQ=60)

                         GTGTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCT                                                                      <  SRR4302532.4753081/94‑1 (MQ=60)

                         GTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCT                                                                      <  SRR4302532.5253698/94‑1 (MQ=60)

                          TCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTG                                                                     <  SRR4302532.5031156/94‑1 (MQ=60)

                          GCTGGAAGGATTAAGTTGCCGGATGATTGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTG                                                                     <  SRR4302532.5520871/94‑1 (MQ=60)

                           CTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGC                                                                    <  SRR4302532.304741/94‑1 (MQ=60)

                           CTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGC                                                                    <  SRR4302532.304742/94‑1 (MQ=60)

                            TGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCG                                                                   <  SRR4302532.6295667/94‑1 (MQ=60)

                            TGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCG                                                                   >  SRR4302532.1542944/1‑94 (MQ=60)

                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGC                                                                  >  SRR4302532.4036922/1‑94 (MQ=60)

                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGC                                                                  >  SRR4302532.6098493/1‑94 (MQ=60)

                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGC                                                                  >  SRR4302532.5047458/1‑94 (MQ=60)

                                AGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGC                                                               >  SRR4302532.1334083/1‑94 (MQ=60)

                                  GATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAA                                                             >  SRR4302532.2768089/1‑94 (MQ=60)

                                   ATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAG                                                            <  SRR4302532.5218056/94‑1 (MQ=60)

                                   ATTAAGGTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAG                                                            <  SRR4302532.1271796/94‑1 (MQ=60)

                                      AAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTG                                                         <  SRR4302532.6267180/94‑1 (MQ=60)

                                       AGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGT                                                        <  SRR4302532.1678762/94‑1 (MQ=60)

                                       AGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGT                                                        >  SRR4302532.5000619/1‑94 (MQ=60)

                                        GTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTA                                                       <  SRR4302532.1061380/94‑1 (MQ=60)

                                           GCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTT                                                    <  SRR4302532.73259/94‑1 (MQ=60)

                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                   >  SRR4302532.714718/1‑94 (MQ=60)

                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                   <  SRR4302532.3201553/94‑1 (MQ=60)

                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                   >  SRR4302532.2834201/1‑94 (MQ=60)

                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                   <  SRR4302532.2040051/94‑1 (MQ=60)

                                             CGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGC                                                  >  SRR4302532.3905643/1‑94 (MQ=60)

                                             CGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGC                                                  >  SRR4302532.2336079/1‑94 (MQ=60)

                                             CGGATGAGGGAAACCTCCGTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CTCTCGTGGTATTTGCGTCTGCGCGGCAAGCTGCACTCGC                                                  >  SRR4302532.5688631/1‑94 (MQ=60)

                                             CGGATGACGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGC                                                  >  SRR4302532.4629106/1‑94 (MQ=60)

                                              GGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCT                                                 >  SRR4302532.1437574/1‑94 (MQ=60)

                                              GGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCT                                                 >  SRR4302532.2809251/1‑94 (MQ=60)

                                              CGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCT                                                 <  SRR4302532.529714/94‑1 (MQ=60)

                                               GATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTTTGCGCGGCAAGCTGTACTTGCTG                                                >  SRR4302532.3633022/1‑94 (MQ=60)

                                               GATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTG                                                <  SRR4302532.1341710/94‑1 (MQ=60)

                                                ATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGC                                               >  SRR4302532.2792451/1‑94 (MQ=60)

                                                 TGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCT                                              <  SRR4302532.2121018/94‑1 (MQ=60)

                                                   ATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCCAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGC                                            >  SRR4302532.1641659/1‑94 (MQ=60)

                                                   ATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGC                                            >  SRR4302532.4514224/1‑94 (MQ=60)

                                                   ATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGC                                            >  SRR4302532.2734251/1‑94 (MQ=60)

                                                    TGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCA                                           >  SRR4302532.1134383/1‑94 (MQ=60)

                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                         >  SRR4302532.3539908/1‑94 (MQ=60)

                                                        AACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGT                                       >  SRR4302532.5280954/1‑94 (MQ=60)

                                                          CCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAA                                     >  SRR4302532.3460314/1‑94 (MQ=60)

                                                          CCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAA                                     <  SRR4302532.4392796/94‑1 (MQ=60)

                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAT                                    >  SRR4302532.3386941/1‑94 (MQ=60)

                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAT                                    <  SRR4302532.2036825/94‑1 (MQ=60)

                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAT                                    >  SRR4302532.2386919/1‑94 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                   <  SRR4302532.5329310/94‑1 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                   <  SRR4302532.997197/94‑1 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                   <  SRR4302532.4152473/94‑1 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                   >  SRR4302532.4001856/1‑94 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                   <  SRR4302532.3825355/94‑1 (MQ=60)

                                                             CCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGC                                  <  SRR4302532.3282952/94‑1 (MQ=60)

                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGTG                                 >  SRR4302532.2357307/1‑94 (MQ=60)

                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCG                                 >  SRR4302532.4517586/1‑94 (MQ=60)

                                                              CTTCTTTAAGTCGCATGTGTCGTTGATCCTC‑GGAAATCGCCCGAGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAgcgtattaca                                 <  SRR4302532.5684353/94‑11 (MQ=60)

                                                                TCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCT                               <  SRR4302532.3284918/94‑1 (MQ=60)

                                                                TCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCT                               <  SRR4302532.1300730/94‑1 (MQ=60)

                                                                 CTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTG                              >  SRR4302532.6042019/1‑94 (MQ=60)

                                                                 CTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTG                              >  SRR4302532.5668926/1‑94 (MQ=60)

                                                                   TTATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCG                            <  SRR4302532.5615363/94‑1 (MQ=60)

                                                                    TATGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGT                           <  SRR4302532.1895572/94‑1 (MQ=60)

                                                                      TGTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGC                         >  SRR4302532.3042714/1‑94 (MQ=60)

                                                                       GTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCA                        <  SRR4302532.1207847/94‑1 (MQ=60)

                                                                       GTCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTCGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCA                        <  SRR4302532.4256890/94‑1 (MQ=60)

                                                                        TCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAC                       <  SRR4302532.1347322/94‑1 (MQ=60)

                                                                        TCGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAC                       <  SRR4302532.1058479/94‑1 (MQ=60)

                                                                         CGCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACC                      >  SRR4302532.1928759/1‑94 (MQ=60)

                                                                          GCATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCA                     <  SRR4302532.4801571/94‑1 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                   >  SRR4302532.5275670/1‑94 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                   <  SRR4302532.6053089/94‑1 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                   >  SRR4302532.5841398/1‑94 (MQ=60)

                                                                            ATGAGTCGTTAATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                   <  SRR4302532.5575963/94‑1 (MQ=60)

                                                                             TGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAT                  <  SRR4302532.5092110/94‑1 (MQ=60)

                                                                             TGAGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAT                  <  SRR4302532.6308938/94‑1 (MQ=60)

                                                                               AGTCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCA                >  SRR4302532.3218392/1‑94 (MQ=60)

                                                                                 TCGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAT              <  SRR4302532.4424045/94‑1 (MQ=60)

                                                                                  CGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATT             >  SRR4302532.3307368/1‑94 (MQ=60)

                                                                                  CGTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATT             >  SRR4302532.5645619/1‑94 (MQ=60)

                                                                                   GTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT            <  SRR4302532.3796470/94‑1 (MQ=60)

                                                                                   GTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT            <  SRR4302532.934755/94‑1 (MQ=60)

                                                                                   GTTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT            <  SRR4302532.1688351/94‑1 (MQ=60)

                                                                                    TTGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTG           <  SRR4302532.5420547/94‑1 (MQ=60)

                                                                                     TGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGA          >  SRR4302532.5730743/1‑94 (MQ=60)

                                                                                     TGATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGA          >  SRR4302532.1529804/1‑94 (MQ=60)

                                                                                      GATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAA         <  SRR4302532.1112465/94‑1 (MQ=60)

                                                                                       ATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA        >  SRR4302532.3662110/1‑94 (MQ=60)

                                                                                       ATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA        <  SRR4302532.5027741/94‑1 (MQ=60)

                                                                                       ATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA        >  SRR4302532.3662109/1‑94 (MQ=60)

                                                                                       ATCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA        >  SRR4302532.3663482/1‑94 (MQ=60)

                                                                                        TCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAAT       <  SRR4302532.3221961/94‑1 (MQ=60)

                                                                                        TCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAAT       >  SRR4302532.3676375/1‑94 (MQ=60)

                                                                                        TCCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAAT       <  SRR4302532.5264630/94‑1 (MQ=60)

                                                                                         CCTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATC      <  SRR4302532.3366140/94‑1 (MQ=60)

                                                                                          CTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC     >  SRR4302532.350875/1‑94 (MQ=60)

                                                                                          CTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC     >  SRR4302532.350874/1‑94 (MQ=60)

                                                                                          CTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC     >  SRR4302532.3767528/1‑94 (MQ=60)

                                                                                          CTCGGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC     <  SRR4302532.4421245/94‑1 (MQ=60)

                                                                                           tcgGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCC    <  SRR4302532.2073245/91‑1 (MQ=60)

                                                                                           tcgGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCC    <  SRR4302532.653909/91‑1 (MQ=60)

                                                                                            cgGCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCG   <  SRR4302532.6014712/92‑1 (MQ=60)

                                                                                            G‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC  <  SRR4302532.4945295/94‑1 (MQ=60)

                                                                                            G‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC  >  SRR4302532.984421/1‑94 (MQ=60)

                                                                                            G‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC  <  SRR4302532.5095244/94‑1 (MQ=60)

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAA‑CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC  >  NC_000913/3930584‑3930769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`