Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F2 I0 R1	193	6708.0	8620653	98.5%	8491343	136.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,678,033	T→G	40.1%	intergenic (+31/‑90)	agn43 → / → CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,678,033	0	T	G	40.1%	13.8 / 26.4	76	intergenic (+31/‑90)	agn43/CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (41/4); new base G (28/2); total (69/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTG  >  NZ_CP022686/4677901‑4678168

gAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCCTCATg                                                                                                                                    >  6:977970/1‑140 (MQ=255)

  aaaTATCACCCTGGGGGTTCTGGCGGTTTTTGCCCACAGCATCAACGGCAGCTGCGCTTATGTTTATAACTGTCAGGCCACACTTAATGTGACCTTCTTATATTTCTGCATTGCCTCTCTGTGGT‑CCCGGTaatcatccc                                                                                                                                  <  2:1035409/140‑4 (MQ=255)

  aaaTATACCCGTGGGCGTTCTTGCGGGTTATTCCCACATAATCAACGTCTGCAGGGATGAAGTTTATAAATGTCAGGCCAAACTTATTTTGACATTATTATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGtcaaattccc                                                                                                                                  <  2:503289/140‑8 (MQ=255)

        cACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCCTGACC‑GGGAc                                                                                                                           >  4:989835/1‑140 (MQ=255)

          cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCAAGAAC‑GGGGccc                                                                                                                         >  1:209112/1‑140 (MQ=255)

               ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCAACAAGACC‑GCGACCCGGagg                                                                                                                    >  7:643402/1‑138 (MQ=25)

               ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTGCCCGGGC‑TCATGCCC‑GGGGCCCCGA‑Cg                                                                                                                   >  3:355670/1‑140 (MQ=21)

               ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGA‑Cg                                                                                                                   >  3:783152/1‑140 (MQ=255)

               ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCAAGCCC‑CGGGCCCGGaag                                                                                                                    >  3:420823/1‑138 (MQ=21)

               ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACC‑GGGACCCGGC‑Cg                                                                                                                   >  7:689606/1‑140 (MQ=255)

               ggCGTTCAGGCCGGTTATGCCAACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTCATGAAC‑GGGACCCGGA‑Cg                                                                                                                   >  3:580932/1‑140 (MQ=255)

                    tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGCCCCGGC‑Cgggggg                                                                                                              >  1:910306/1‑139 (MQ=255)

                     cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATACTGACC‑CGGACCCCGC‑CGGGGGaa                                                                                                             >  8:378266/1‑140 (MQ=25)

                     cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATAATGACC‑GGGACCCGGA‑CGGGGGaa                                                                                                             >  4:35191/1‑140 (MQ=255)

                     cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCGTCATTCCCGGGGACCGGCA‑Cgggggt                                                                                                              >  2:924040/1‑139 (MQ=21)

                     cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGC‑CGGGGGaa                                                                                                             >  4:427123/1‑140 (MQ=255)

                     cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATAATGACC‑GGGCCCCGGC‑CGGGGGaa                                                                                                             >  4:1012485/1‑140 (MQ=255)

                             ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCAC‑GGGACCCCGC‑CGGGGGCAAACggata                                                                                                     >  2:598415/1‑136 (MQ=25)

                                  cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTCATGACC‑GGGACCCCGA‑CGGGGGAAAACGTTTAAGTGc                                                                                                >  4:762299/1‑140 (MQ=255)

                                      caGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCAAAAAGACC‑GGGGCCCGGG‑CGGGGGGGAACGGTTAACTGCcaca                                                                                            >  8:540875/1‑140 (MQ=21)

                                       aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCTCATGCCC‑GGGACCCGGG‑CGGGGGTAAACCGTTGAGTggcccaa                                                                                           >  3:499741/1‑134 (MQ=25)

                                       aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATAATGACC‑GGGGCCCGGA‑CGGGGGAAAACGGTTCAGGGCcacct                                                                                           >  4:193660/1‑138 (MQ=37)

                                       aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAATGACC‑GGGGACCGGA‑AGGGGGTAAACGTTTAAGTGCcacaa                                                                                           >  7:124615/1‑139 (MQ=25)

                                           tCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTGCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCACATTACC‑GGGACCCGGA‑CGGGGGAAAACGTTTGAGTGCCACAttaca                                                                                       >  6:944787/1‑137 (MQ=37)

                                               cGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCGTCAAGACC‑GCGACCCGGA‑CGGGGGAAAAACTTTAAGTGCCCAATTCACAGAc                                                                                   >  1:866149/1‑140 (MQ=25)

                                               cGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCAACAAGACC‑GGGAACCGGA‑CGGGGGAAAAACTTTACCTTCCACATTAACAGAc                                                                                   >  1:870545/1‑140 (MQ=21)

                                                 gcagcaGCGCTGAATGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCAGCAAGAAA‑CGGCCCCGGC‑CGGGGGTAAACGTTTCCGTGGAACATTAAcagccaa                                                                                 >  5:357171/1‑134 (MQ=255)

                                                   agcagtGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGAAC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCCCATAAACTGACAtta                                                                               >  4:115417/1‑139 (MQ=37)

                                                   agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCCGTCAACCTTACC‑CGGACCCCGA‑CGGGGGGAAACGTTTAAGTGCCCCATTAAATGACAttg                                                                               >  4:170957/1‑139 (MQ=25)

                                                   agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACCTTCACTGACATTc                                                                               >  4:87093/1‑140 (MQ=255)

                                                   agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCGCTCTGTGGT‑CCCGGGCATCCTGCCC‑GGGCCCCCGC‑GGGGGGAAACCGTTTCAGTGCCACATTCACTGACCTTc                                                                               >  6:10197/1‑140 (MQ=37)

                                                       gcgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGGCC‑GGGACCCCGA‑CGGGGGAAAACGTTTCAGTGACACATTAACTGCCATTCACaa                                                                           >  1:846332/1‑140 (MQ=255)

                                                        cgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGGTTCAGTGCCCCAATCACTGACATTCACAAt                                                                          >  8:784182/1‑140 (MQ=255)

                                                               ggTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTTATAATGACA‑GGGGCCCGGA‑CGGGGGAAAAAGTTTCAGTGACACATTCACTGACCTTCACAAGAAAATgt                                                                   >  2:897514/1‑139 (MQ=38)

                                                                  tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGTT‑CCCGGTCAGAATGACC‑GGGACCCGGA‑CGGGGGTAAACGTTTCCGTGCCACCTTAACTGACTATCACAATAACAAGatat                                                                >  8:807821/1‑140 (MQ=37)

                                                                  tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCAAATTCACGGACATTCACAAAAACATGatat                                                                >  8:680018/1‑140 (MQ=255)

                                                                  tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCC‑GGGACCCGGC‑CCGGGGAAAACGTTTCAGTGCCCCATTCACTGACATTCACAATAACATGatat                                                                >  4:397171/1‑140 (MQ=255)

                                                                  tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACAGACATTCACAATAACATGatat                                                                >  4:1076982/1‑140 (MQ=255)

                                                                       cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGAAC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTTCCACATTCACTGACATTCACAATAACATGATATtaatc                                                           >  7:640500/1‑140 (MQ=255)

                                                                       cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑CGGACCCCGA‑CGGGGGAAAACGTTTAAGTGCCACATTCACTGACATTCACAATAACATGATATtaata                                                           >  6:337733/1‑139 (MQ=255)

                                                                         gTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGC‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAAATACATGATATTCATCAc                                                         >  6:789694/1‑140 (MQ=255)

                                                                          tCAGGCCACACTTAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACC‑GGGAACCGGA‑CTGGGGAAAACGTTTAAGTGCCACATTAAAGTAACTTCACAATAACATGATAATCATAAcc                                                        >  6:935058/1‑139 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATGACC‑CGGCCCCGGC‑CGGGGGCAAACCTTTCAGTGCCACAATCACAGAACATAAAAAAAACATGCTATTAATAACg                                                        >  1:1060637/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCAAGACC‑GGGACCCGGC‑CCGGGGTAAACGTTTAAGTGCCACATTCACTGACATTCACAATAACATGATATTAATCACg                                                        >  3:769302/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCAAGACC‑GGGACCCGGA‑CCGGGGAAAACGTTTCACGGCCCCCTTAACTGACATTCAAAATAACATTATATTCATAACg                                                        >  2:412355/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCAGGACC‑GGGACCCGGA‑CGGGGGAAACCGTTTCAGTGCCACAATGACTGACATTCACAATAACATGATATTAATGACg                                                        >  4:428186/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGGCC‑GGGGCCCGGC‑AGGGGGTAAACGTTTCAGTGCCCCAGACACTGGCCTTCTCAATAACAAGATATtcaaaacg                                                        >  6:962322/1‑135 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GCGCCCCCGACCGGGGGAAAAC‑TTTCAGTGCCACAATAACTGACATTAAAAAAAACATGATATtaataaac                                                        >  7:58245/1‑136 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCAATCAC‑GGGCCCCGGG‑CGGGGGTAAAAGTTTAAGTGCCACATTGACAGACATTCACAATAACAAGATGATAATCACg                                                        >  2:1089000/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGATCATCGCC‑GGGGCCCCGC‑CGGGGGGAATCGGATCATGGGCCCCTTCACGAACATTCACAATAACATGATATTAATCACg                                                        >  8:658118/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGG‑CGGGGGGAAACGTTTCAGTGCCACATTTACTGACAGTCACAATAACAATATATTAATCACg                                                        >  6:498036/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGCCC‑GGGGCCCGGA‑CGGGGGAAAACGTTTAAGTGCCCCCTTAACTGCCATTCACAATAACATGATATTAATAACg                                                        >  2:1093734/1‑140 (MQ=255)

                                                                          tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGACC‑GGGCCCCGGG‑CGGGGGAAAACGGTTCCGAGCCACCTTCACTGGCATTAACAAAAACATGATATtcatgaag                                                        >  4:1071983/1‑136 (MQ=255)

                                                                                  catttattgtgAACTTTTTATAATTTTTCATTCCCTCTGTGTGGT‑CCTGGTCATAATTCCG‑GGTACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                <  6:798788/133‑1 (MQ=255)

                                                                                  cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTAATACCGGAgggcct                                                >  8:340662/1‑135 (MQ=255)

                                                                                  atcttaaTGTGACATTCTGATATTTTTGCATTGCCTCTGTGGGGC‑CCGGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                <  8:124615/135‑1 (MQ=255)

                                                                                  aacttattgtgACCTTCTTATATTTCTCCATTGCCTCTCGGGGGC‑CCCGTGCCTCATGCCC‑GGCACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                <  6:509338/133‑1 (MQ=255)

                                                                                   acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATTACC‑GGGCACCGGC‑CGGGGGCAAACGGTTGAGGGGCACCTTCACTGACATTCACAATTACATGAGATTCATCACGGAGTGagtt                                               >  5:74285/1‑137 (MQ=255)

                                                                                   acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATAATGACC‑GGGACCCGGA‑CGGGGGGAAACGTTTCAGTGGCCCATTAACTGACATTCACAATAACATGATATTAATAACGGAGTGACTa                                               >  4:521875/1‑140 (MQ=255)

                                                                                             aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATCATGTCC‑CGGACCCCGA‑CGGGGGCAAACCGTTCAGTGCCACAATAACAGACATTCACAATAACAACATAATAAACACGGAGTTACAATTTTaaataa                                     >  2:199114/1‑135 (MQ=255)

                                                                                               cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACC‑GGGCCCCGGC‑CGGGGGCAAAACTTTCAGTTCCACATTCACTGCAATTCACAATAAAATGATATTAATCAAGGAGTGGATATGTTAAACATat                                   >  7:438464/1‑139 (MQ=255)

                                                                                               cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGACC‑CGGACCCGGA‑CGGGGGAAAACGTTTAAGTGCCACATTCACAGACATTCACAATAACATGATATTAATCACGGAGTGCCTATGTTAAAAATAg                                   >  8:452979/1‑140 (MQ=255)

                                                                                               cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACC‑GGGGCCCGGA‑CGGGGGGAAACGTTTAAGTGGCACATTCACAGACCATAACAAAAAAATGATATTAATAACGGAGTGACTTTGTTTCAGAttt                                   >  5:325004/1‑138 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCACATTACC‑GGGACCCGGG‑CGGGGGAAAACGGTTCCGTGCCACAGTCAAGGACAATAACAAAAAAATGATATATATCACGGAGGGACTAATTTAGAAATAGta                                 >  6:174560/1‑139 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGTAAACGGTTCAGTGCCACATTAACTGACATTAACAATAACAATATATTAATAACGGAGTGACTATGTTACAGATatta                                 >  3:986983/1‑137 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGACC‑GGGACCCGGA‑AGGGGGAAAAAGTTTCAGTTACACATTAACTGACATTCACAATTACATGATATTAATCACGGATTTACTATTTTAAAGATattt                                 >  2:663407/1‑137 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGATCATGAAC‑CGGACCCGGG‑CGGGGGAAAAAGTTTACGTTCCAAATTCACAGGCATTAAAAATAACATTAAATTAATCAAGGATGGACAATGTTACAtatattg                                 >  2:784796/1‑137 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGCCCCGGA‑CGGGGGGAAACGTTTAAGTGCCCCATTAACTGACATTCACAATAACATTATATTAATAACGGAGTGACTATGTTACAGATAGta                                 >  7:1000890/1‑139 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGACC‑GGGACCCCGA‑CGGGGGAAAACGTTTAAGTTCCACATTCACAGCAATTAACAAAAACATGATATTAATAAACGAGTGACTATTTTACAGATAGta                                 >  1:1078527/1‑139 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCATGACC‑GGGGACCGGG‑CGGGGGAAAACGTTTTAGGGCCACCTTAACGGACATTAAACATAAAATGTTTTTCATCAAGGGGTGCCTATTGTACAGAGAGtg                                 >  5:772458/1‑139 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCCTCATGACC‑GGGCCCCCGC‑CGGGGGAAAACGTTTCAGTTCCACAATAACGGACATTAACAATAAAATGATATTAATCACGGTGTGCCTATGTTTCAGaatgta                                 >  2:353372/1‑135 (MQ=255)

                                                                                                 tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAAAGACC‑CGGGCCCCGA‑CGGGGGAAAAACGTTCAGTGGCCCAATCACAGACATTAACAATAACATGATATTAATAACGGAGTGTCTATGTTTAAGATAGTc                                 >  5:485640/1‑140 (MQ=255)

                                                                                                   ttaaaaTTCTGATTGGCCTCCGTGGGGC‑CGCGGTCATCATGCCG‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                               <  2:1010512/136‑1 (MQ=255)

                                                                                                            tGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGA‑CCGGGGAAAACGGTTCAGTGCCACATTCACTGACAATCCCAATAACAAGATATTAATAAAGGAGTTAATATGTTACAGATATTAGGCGCGCTTAt                      >  6:944924/1‑140 (MQ=255)

                                                                                                                      ctctGGTGT‑CCCGGCCCACACGACG‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATc            <  6:949396/140‑1 (MQ=255)

                                                                                                                         tgtgGG‑CCCGGGCATCATGACC‑GGGGCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCa         <  8:250739/140‑1 (MQ=255)

                                                                                                                                 cccgTCATCAAGACC‑GGGACCCGGA‑CGGGGGAAAAAGTTTCAGTGCCACATTAACTGACATTCAAAATAAAATTATATTAAAAAAGGATTGACTATTTTTCAGATATTAGGCGCGCGGCTTCTTCTGATAGCAGGAtttt  >  2:544700/4‑139 (MQ=255)

GAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTG  >  NZ_CP022686/4677901‑4678168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A