Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F13 I0 R1	230	5035.0	9125430	98.4%	8979423	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,678,041	A→C	20.8%	intergenic (+39/‑82)	agn43 → / → CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,678,041	0	A	C	20.8%	50.4 / 8.6	79	intergenic (+39/‑82)	agn43/CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (55/4); new base C (8/7); total (68/11)
Fisher's exact test for biased strand distribution p-value = 7.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

TATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCG  >  NZ_CP022686/4677906‑4678178

tatCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATAATTACCgg                                                                                                                                         >  6:278134/1‑140 (MQ=255)

        tGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑ACCCggc                                                                                                                                >  6:312422/1‑139 (MQ=255)

          ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATATCAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑ACCCGGaag                                                                                                                              >  2:481662/1‑138 (MQ=255)

          ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCAGGACCGGG‑ACCCGGCCg                                                                                                                              >  5:265719/1‑140 (MQ=21)

          ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCTCATGCCCCGG‑ACCCggc                                                                                                                                >  8:757873/1‑137 (MQ=255)

          ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGAGGT‑CCCGGGCAACAAGACCGGG‑ACCCGGACg                                                                                                                              >  2:852573/1‑140 (MQ=255)

          ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCCCTGTGGT‑CCCCGGGATCATTACCGGG‑GCCCCGAcc                                                                                                                              >  5:524158/1‑139 (MQ=21)

          ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGAATTGCCTCTCTGTGGT‑CCCGGTCATCAAGACCGGG‑CCCCGGACg                                                                                                                              >  5:266266/1‑140 (MQ=255)

          ggCGTTCAGGCCGGTTATGACCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGCCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGACAACCAGCCCGGG‑ACCCgggcc                                                                                                                              >  5:101267/1‑137 (MQ=21)

            cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACg                                                                                                                              >  7:723301/1‑138 (MQ=255)

            cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCCAAATGACCGGG‑GCCCGGACg                                                                                                                              >  7:517814/1‑138 (MQ=21)

              ttCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATAAAGACCGGG‑GCCCGGCCggggg                                                                                                                          >  3:369404/1‑140 (MQ=25)

               tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCAAAATGACCGGG‑CCCCGGACGGGGGa                                                                                                                         >  6:862649/1‑140 (MQ=255)

               tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑ACCCGGACGGGGGa                                                                                                                         >  6:343782/1‑140 (MQ=255)

                cAGGCCGGTTATGCCCACAGCATCACCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATTCCCGGG‑CCCCCGAAGGGGGag                                                                                                                        >  3:744836/1‑139 (MQ=21)

                cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGCAGGTTATAACAGTCTGGCCACCCTGAAGGGGCCCGTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCAGAATGACCCGG‑GCCCGGGCGGGGGac                                                                                                                        >  5:1100775/1‑139 (MQ=25)

                cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATGACCGGG‑ACCCGGCCGGGGGaa                                                                                                                        >  5:263148/1‑140 (MQ=255)

                cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCCTTACCCGG‑ACCCCGTCgggggca                                                                                                                        >  1:644861/1‑138 (MQ=25)

                cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTTACGGG‑ACCCGGCCGGGGGaa                                                                                                                        >  3:744829/1‑140 (MQ=255)

                         tATGCCCACAGCATCAACGGCAGCATCGCTGAAGGTTATTACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGTTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCa                                                                                                               <  3:152803/140‑1 (MQ=255)

                               cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCAGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCca                                                                                                         >  5:553191/1‑140 (MQ=255)

                               cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATTCCCGGG‑ACCCGGACGGGGGAAAACCtttaatagc                                                                                                           >  8:542792/1‑132 (MQ=25)

                                  aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCcacaa                                                                                                      >  2:1104023/1‑139 (MQ=255)

                                  aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑CCCCGGACCGGGGAAAAACTTTAAGTGCCACAt                                                                                                      >  1:753635/1‑140 (MQ=25)

                                    cATCAACGGCCGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCCGG‑CCCCGGCCGGGGGCAAACGTTTGAGTGGCACAt                                                                                                      >  7:199590/1‑138 (MQ=37)

                                    cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGGCA‑GATGGACGGG‑ACCCCGGCCGGGGGAAACGGTTGAGTTCCCCAt                                                                                                      >  7:243088/1‑138 (MQ=18)

                                    cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCCGGG‑ACCCGGCCGGGGGAAAACGTTTCAGTGCCACAt                                                                                                      >  8:742474/1‑138 (MQ=255)

                                     aacAACGCCAGCCGCGCTTATGGTAAAAACTGTCCGCCCAAACTCAATGTCACCTTCTTAAAATTCTCCATTCCCTGTCTGTGGC‑CCCGGTCATCTTGCCGGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCa                                                                                                   <  2:838806/138‑1 (MQ=255)

                                              agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAAAGTTTGAGTGCCACATTCACTGACAtta                                                                                          >  5:694672/1‑139 (MQ=255)

                                                cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCCGG‑ACCCGGGCGGGGGAAAACGTTTCAGTGCCACAGTCACTGACATTcac                                                                                        >  2:888844/1‑140 (MQ=255)

                                                cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGCTCATTGCCCGG‑GCCCGGGCGGGGGGAAACGGTTTAGTGCCCCAGTGACTGACAtta                                                                                          >  8:222998/1‑137 (MQ=21)

                                                cagcGCTGAAGGTTATAACAGTAAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCTCCAGCCCGGG‑CCCCGGACGGCGTAAAACGTTTAACTGGCACATTCACTGaaaatg                                                                                          >  8:250605/1‑133 (MQ=25)

                                                             tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCGGGCAACATGACCGCG‑CCCCGGGCGGGGGAAAACAATTAAGTTCCCCAATACCAGAAATAAACAAAAACA‑TGaaat                                                                          >  6:233993/1‑140 (MQ=255)

                                                               taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGGCCGGG‑ACCCGGGCGGGGGAAAACGTTTCAGTGGCACAGTAACTGACATTCACCATAACA‑TGctat                                                                          >  7:255954/1‑138 (MQ=255)

                                                                 aCAGACCGGCCACACTCAATGTCACATTCTGAAATTTCTGCATTGCCTGTGTGTGGC‑CCCGGTCACCA‑GCCCGGGCCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATtcat                                                                      <  4:1111318/140‑1 (MQ=255)

                                                                  cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATGACCGGG‑ACCCGGGCGGGGGAAAACGGTTCAGTGCCACATTCACTGACATTCACAATAACA‑TTATATtaata                                                                     >  6:473292/1‑139 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑GCCCGGCCGGGGGAAAACGTTTAAGTGCCACATTCACTGACATTCACAATAAAA‑TGATATTCATCAcc                                                                  >  1:888488/1‑139 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACg                                                                  >  6:1006608/1‑140 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTCATTACCGGG‑GCCCGGACGGGGGAAAACGGTTCAGTGGCCCATTCACTGACCTTAACAAAAACA‑TGATATTAATCACg                                                                  >  1:608423/1‑140 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCACATGCCCCGG‑CCCCGGCCGGGGGAAAACCTTTCAGGGCCCCATTCACTGACATTCACAAAAACA‑TGATATTCATcgcg                                                                  >  3:1037652/1‑140 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCAAGCTTACCGGG‑GACCCGACGGGGGAAAACCTTTAAGTGCCACAATCAATGTCATTAACACAAACA‑TTAGATTAATAACg                                                                  >  1:749052/1‑140 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGCTCATGACCCGG‑ACCCGGCAGGGGGTAAACGTTTCAGTGCCACAGTCACTGACATTCACCATTACA‑TGATTTtaatcaaa                                                                  >  1:1035661/1‑138 (MQ=255)

                                                                     tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTACTCATGACCGGG‑GCCCGGGCGGGGGAAAACGGTTCAGTGCCCCCTTCACTGACCTTAACAATAACA‑TGTAATTCATCACg                                                                  >  6:262310/1‑140 (MQ=255)

                                                                      cAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCAGGTCATCAAGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTAACAATAACA‑TGATATTCATCACgc                                                                 >  6:913133/1‑139 (MQ=255)

                                                                       aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑GCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAAAA‑TGATATTCATCAcc                                                                  >  7:156867/1‑137 (MQ=255)

                                                                         gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATTACCGGG‑TCCCGGAACGGGGCAAAAGTTTGAGTTGCCCCTTCAGAGTCAATGACAATAACA‑ATATTTTACTCCCGGAGt                                                              >  5:537731/1‑140 (MQ=255)

                                                                         gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCAAGACCCGG‑CCCCCGCCGGGGGAAAAAGTTTGAGTGGCCCATTAAATGACATTGCCAATAACA‑TGATTTTAAACAAGGAGt                                                              >  3:1010069/1‑140 (MQ=255)

                                                                         gCCACACTCAATGTGACCTTCTGATAATTCTGCATGCCCTCTGTGTGGC‑CCCGGTAATCATCACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGGGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGa                                                                <  8:168427/138‑1 (MQ=255)

                                                                           cacacaGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGCCCGGG‑ACCCGGAAGGGGGAAAACGTTTCAGTGCCACATTCACGGACATTAGCAATAACA‑TGATATTCATCACGGAGt                                                              >  8:645182/1‑138 (MQ=255)

                                                                           cacaCTTATTGTGACATTCTTATATTTCTGCATTGCCTCTCTGTGGG‑CCCGGTCATCATCCCGGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGt                                                              <  8:723301/138‑1 (MQ=255)

                                                                             cacTTAATGTGACATTCTGAAAATTCTGCCTTCCCGCTCTGGGGT‑CCCGGGCAAACTGCCCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACt                                                          <  4:691060/140‑1 (MQ=255)

                                                                             cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑GCCCGGACGGGGGAAAACGTTTGAGTGGCACATTCACGGACATTCACAATAACA‑TGATATTAATAACGGAGTGACt                                                          >  3:64249/1‑140 (MQ=255)

                                                                              acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCAAGACCGGG‑ACCCGGGGCGGGGAAAACGTTTAAGGGCCACATTCACAGAGATTCACAATAAAA‑TTATATTAATAACGGAGTTAcaa                                                         >  4:371245/1‑138 (MQ=255)

                                                                                  aaTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGtt                                                     >  4:638988/1‑140 (MQ=255)

                                                                                        aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCCGTCCTTCTTACCGGG‑ACCCGGGAGGGCGCAAACGTTTGACTGACCCAAAGACTGGAATT‑ACCAAAACATTTATATTAATCCCGGATGGACAATGTTACactt                                               >  2:39753/1‑137 (MQ=255)

                                                                                        aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATTACCCGG‑ACCCGGCCCGGGGAAAACGTTTCAATGCCCCATTACCAGACATTCACAAAAACA‑AGATATTAATCAAGGCATTACTATGTTACAAAt                                               >  6:339695/1‑140 (MQ=255)

                                                                                         ccTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACCGGG‑ACCCGGCCGGGGGGAAACGTTTAAGTGCCACAGTCACTGGCATTCAAAATAACA‑ATATAATAAAAACGCCGTGAGCATGTTACAGa                                                >  8:139482/1‑138 (MQ=255)

                                                                                          cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑ACCCGGACGGGGGATAACGTTTCAGTGCCCCATTGACTGCCATTCACAATAACA‑TGATATTAATCACGGAGTGACTATGTTACAGATat                                             >  6:1014641/1‑139 (MQ=255)

                                                                                            tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCAATACCCGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCCCATTCCCAGCCATTAACAATAAAA‑AAATATTAAACACGGGGGTACGATTTTACAAATAGtg                                           >  2:383529/1‑139 (MQ=255)

                                                                                            tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTAATGACCCGG‑ACCCCGCCGGGGGAAAACGTTTAAGTTCCCCAATCACAGACAATCACAAAAAAA‑AGATATTAATCAACGAGTGACTATTTTTCAGATatta                                           >  6:219839/1‑137 (MQ=255)

                                                                                            tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTAATAAATACCCGG‑ACCCAGACGGGGGAAAACATTTCATTGCCCCATTCACTGACAATAACAAAAACA‑TAAAAATAATAACCGAGTGACTATTTTACAGATAGTc                                           >  3:466709/1‑140 (MQ=255)

                                                                                            tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCATGACCGGG‑GACCGGGCGGGGGGAAAAGGTTAAGTGCCACATTAACTGACATTCACAATAAAA‑TGATATTAATAACGGAGTGACTATGTTTAAGATAGta                                           >  6:704868/1‑139 (MQ=255)

                                                                                            tCTGAAAATTCTGCATTGCCTCTCTGTCGT‑CCCCGGAATCAAAAACGGG‑ACACGGAAGGGTGAAACAGATTCAGTGCCACAATCACAGACATTAACAATAACA‑TCATATTAATCTCGGAGAGACTATGTTACAGaatgta                                           >  4:900303/1‑135 (MQ=255)

                                                                                              ttaTAATTCTCCATTGCCGCTCTGGGGG‑CCGGGTCATCCGGCCGGGG‑CCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                         <  4:862857/138‑1 (MQ=255)

                                                                                              tGATAATTCTGCATTGCCTCTCTGTGGTGCCCGGGC‑TCATGGCCCGG‑GCCCGCGCGGGGGGAAACGGTTAAGGGTCACCATAACAGACAATAACAATAACA‑ATATATTAAACACGGCGTGGGAATGTTAAAGaaattg                                           >  8:782619/1‑133 (MQ=255)

                                                                                              tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTAACTGACATTCACAATAACA‑TGATATAGATCACGGAGTGACTATGTTTCAGATTGtg                                           >  8:677347/1‑137 (MQ=255)

                                                                                              tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATAATGACCGGG‑ACCCGGCCGGGGGAAAACGTTTGAGTGCCACCATAACTGACAATCACAATAACA‑TGATATTAATAACGGAGTGACATTTTTACAGATATTCgg                                         >  4:742619/1‑140 (MQ=255)

                                                                                              tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑GCCCCGGCCGGGGATAAAGTTTCAGTGGCCCAATCACTGACAATCCAAATAACA‑TGAGATTACTCACGGGGTGAAGATGTTTCAGATAGtg                                           >  8:372663/1‑137 (MQ=255)

                                                                                              tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATAATGACCGGG‑ACCCGGCCGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTAAAGATAGta                                           >  8:185808/1‑137 (MQ=255)

                                                                                                      ttctaTTGCCTGTCTGTGCC‑CCCGGACATCATCCCCGGT‑ACCCTGAAGGTGTAAAACGTTTCAGTGCCAAATTCACTGACTTTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                   <  7:549954/134‑1 (MQ=255)

                                                                                                        gCATTGCCTGTGTGTTGT‑CCCGGTCATCATCACCGGT‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAtt                               <  4:643096/140‑1 (MQ=255)

                                                                                                                tctctGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGTCGGGGGAAAACGTTTAAGTGCCCCATTCACTGACATTAACAATAACA‑TGATATTAATCACGGAGTGACTATGTTACTGATATTCGGCGCGCTGATTctggtg                         >  8:253922/1‑138 (MQ=255)

                                                                                                                 ctctGTGGT‑CCCGGTCATAATGACCGGG‑CCCCGGACGGGGGAAAACCTTTCATTTCCACATTAAAATAAATTCACCATAAAA‑TGAAAATAATAAATGTGTGCCTATGTTACAACTAGTCGGCCTCCTGATTatggagagg                      >  4:444382/1‑134 (MQ=255)

                                                                                                                   ctGTGGT‑CCCCGTGATCATGACCGGG‑CCCCCGGCGGGGGGAAACATTTAAGTTCCCCATTAACTGCAATTCACAATAACA‑TGATATTAATAACGGAGTGACTATGTTAAAGATATTAGGCGCGCAGAATCTGCTGCTCGc                    >  2:108508/1‑140 (MQ=255)

                                                                                                                         t‑CCCCGTGATAATGACCCGG‑ACCCGGACGGGGGAAAACGTTTAATTGCCCCATTCACATACATTAACAAAAACA‑ATATATTAATAAACGACTGAATATGTTAAAGAAATTAGGCGCGCTGATTATGCTGATAGCAGGAtt              >  6:840383/1‑140 (MQ=255)

                                                                                                                              ggTCATCATGCCCGGC‑CCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGcc          <  4:164894/140‑1 (MQ=255)

                                                                                                                                   tcatGACCGGG‑ACCCGGACGGGGGAAATCGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTTATCGAAGGATTTGCCAttct     >  1:852064/1‑140 (MQ=255)

                                                                                                                                   taattaCCGGG‑ACACGGACGGGGGAAAACTTTTCAGTTCCACAGTCACTGACATTCACAATAACA‑TGATATTCATAACTGAGAGAATATGTTAAAGATAGTCGGCGCGCTGATTCTGCTGATCGCATGAATTGCAAttcg     >  1:852059/6‑139 (MQ=255)

                                                                                                                                     attaCCGGT‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct     <  8:134291/135‑1 (MQ=255)

                                                                                                                                     atGACGGGG‑ACCCGGACGGGGGAAAACGTATCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc   <  4:859580/140‑1 (MQ=255)

                                                                                                                                      tGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGACATTCTTCg  >  3:1046551/1‑140 (MQ=255)

TATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCG  >  NZ_CP022686/4677906‑4678178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A