Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F2 I0 R1 233 6461.8 9239822 98.3% 9082745 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,041 A→C 17.1% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0410AC17.1% 77.2 / 3.0 97intergenic (+39/‑82)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (71/5);  new base C (9/8);  total (84/13)
Fisher's exact test for biased strand distribution p-value = 1.86e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCG  >  NZ_CP022686/4677904‑4678178
                                                                                                                                          |                                                                                                                                            
aaTATCACGCTGGGGGTTCAGGCGGGTTATGCCCACAGCATCAACGGCAGACGCGCTGAAGGTTATAACAGTCAGGCCACCCTTAAGGTGACCTTCTCATAATTCTGCAGTGCCGCTCTGTGGT‑CCCGGTCATCATGAcc                                                                                                                                            <  8:914293/140‑1 (MQ=255)
aaTATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGAATTGCCTCTCTGTGGTGCCCGGTCA‑CATGaac                                                                                                                                            >  8:415357/1‑138 (MQ=18)
       cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑A‑ccc                                                                                                                                   >  8:184499/1‑140 (MQ=255)
       cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGACCGGG‑A‑cgc                                                                                                                                   >  3:346582/1‑138 (MQ=255)
          tGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGAGCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑T‑CCCggc                                                                                                                                >  2:757593/1‑139 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTTAATGAGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCAGAATGACCGGG‑G‑CCCGGCCg                                                                                                                              >  2:247131/1‑140 (MQ=21)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATGCCCGGG‑A‑CCCGGACg                                                                                                                              >  7:617829/1‑140 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGCCg                                                                                                                              >  2:495826/1‑140 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATAATGACCGGG‑A‑CCCGGGCg                                                                                                                              >  8:142557/1‑140 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCAACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACGTAATGTGCCCTTCTGATAATTCTTCATTGCCTCTCTGTGTT‑CCCGGTAATAATGACCGGG‑T‑CCCGGACg                                                                                                                              >  8:345982/1‑140 (MQ=21)
              cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑ccccgcag                                                                                                                              >  5:986929/1‑133 (MQ=255)
              cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGACTAATGCCCGGG‑A‑CCCGGACg                                                                                                                              >  5:6603/1‑138 (MQ=21)
              cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCGTCATGACCCGG‑c‑ccccgcac                                                                                                                              >  5:6601/1‑133 (MQ=21)
                            aTGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATGACCGGG‑G‑CCCGGACGGGGGAAAACGttta                                                                                                                >  6:685854/1‑137 (MQ=255)
                               cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGGCCGGG‑A‑CCCGGGCGGGGGAAAACGGTTAAGTgg                                                                                                           >  1:650065/1‑139 (MQ=37)
                               cccACACCATCACCGGCAGCTGCGTTTATGGTTATACCAGCCCGGCCACATTCATTGTCAACTTCTTATATTTCTCCATGCCCCGTCTGGGGC‑CCCGGCCATCACCCCGGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGt                                                                                                             <  6:638897/138‑1 (MQ=255)
                                   caGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCAAGACCCGG‑A‑CCCGGACCGGGGAAAACGATTCCGTGCcacc                                                                                                       >  7:703362/1‑139 (MQ=37)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATACTTAAAGGG‑T‑CCCGGACGGTGGTTAACGGTTAAGttcagcat                                                                                                      >  3:47895/1‑133 (MQ=21)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCCGGG‑C‑CCCGGGCGGGGGGAAACGGGTCAGTGCCCCAt                                                                                                      >  7:1009873/1‑140 (MQ=37)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAt                                                                                                      >  4:1062250/1‑140 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAt                                                                                                      >  4:52859/1‑140 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCCGG‑C‑CCCGGACGGGGGAAAACGTTTAAGTGGCCCAt                                                                                                      >  3:859204/1‑140 (MQ=37)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAATGACCGGG‑A‑CCCGGCCCGGGGGAAACCTTTAAGTggcccaa                                                                                                      >  4:200779/1‑134 (MQ=25)
                                         cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CTCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACt                                                                                                 >  2:1087484/1‑140 (MQ=255)
                                         cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTAAcc                                                                                                 >  2:946881/1‑139 (MQ=255)
                                         cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCCGACGGGGGAAAACGTTTAAGTGCCACATTAACt                                                                                                 >  2:495959/1‑140 (MQ=255)
                                              gcagcaGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAAATCTGCATTGCCTCTCTGTGGT‑CCCCGTGATAATTACCGGG‑A‑CCCGCAAGGGGGACAACCAATCACTGCCACCTTAACAGaa                                                                                              >  5:828508/1‑137 (MQ=21)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACCTTTCAGTGCCACCTTACCAGACAtta                                                                                          >  2:1154602/1‑139 (MQ=37)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑C‑CCCGGCCGGGGGAAAACGTTTAAGTGCCACAATCACTGACaata                                                                                          >  2:204210/1‑137 (MQ=37)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGCTCATGACCCGG‑A‑CCCGGACGGGGGGAAACGTTTCAGTGCCCCATTCAATGACaata                                                                                          >  4:3826/1‑137 (MQ=37)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAATTCCCGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTCACTGACaa                                                                                            >  5:266029/1‑137 (MQ=37)
                                                 gctgcgcTGATGTTTATAACAGTCCGGACACACTTATTGTCACCTTCTTATAATTCTGAATTCCCTCTGTGTGGG‑CCCGGCCATCATGCCGGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTca                                                                                         <  4:419906/137‑1 (MQ=255)
                                                 gcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCAAGACCGGG‑A‑CACGGACGGGGGAAAAACTTTCAGGGCCACAATAACTGACATTca                                                                                         >  1:856831/1‑140 (MQ=37)
                                                  cagcGCTGAAGGTTATAACAGTCAGGCCACACTGCATGTGACCGTCTGATAATTCTGCATTTCCTCTCTGTGGT‑CCCGGTCATGAAGCCCGGG‑C‑CCCGGCCGGGGGACAACGTGTCAGTGACCCATTCACTGAccatc                                                                                          >  6:1062095/1‑134 (MQ=25)
                                                  cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGAACATGACCGGG‑A‑CCCGGACGGGGGAAAAAGGTTCAGTTCCACCTTCAGAGACATTc                                                                                          >  5:661366/1‑138 (MQ=37)
                                                   agcTCTGAAGGGTAACACAGTCCGGCCACACTGAATGTGAACTTCAGATATTTCTTCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGTG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTcaca                                                                                       <  7:680123/140‑1 (MQ=255)
                                                      gcTGATGTTTAAAACAGCCCGCCCACACTGATTGGTACCTTCTTATAATTCTGATTTGCCTCTCTGTGGC‑CCCGGCCATCATGCCGGGT‑C‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATa                                                                                   <  2:529872/140‑1 (MQ=255)
                                                       cTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑C‑CCCCGGCGGGGGGAAACGTTTAAGTGGCACATTCACTGACATTAACa‑aa                                                                                    >  5:1082259/1‑137 (MQ=255)
                                                           cggTTATAACAGTTATCCCACTCTCATTTTAACTTTCTTTTAATTTTGCATTGCCACTTTTGGGC‑CCGGGAAATCAGCACGGGC‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATg                                                                              <  4:667783/139‑1 (MQ=255)
                                                               tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑G‑CCCGGCCGGGGGCAAACGGTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGatat                                                                          >  2:1167806/1‑140 (MQ=255)
                                                               tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGGCGGGGGAAAACGTTTCAGTGCCACAATGACTGACATTCACA‑AAAACATGaaat                                                                          >  4:543671/1‑140 (MQ=38)
                                                               tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CACGGTCAAGATGACCCGG‑A‑CCCCGACGGGGGAAAAAGTTTCAGTGCCACAATAACGGACATTCACA‑ATAACAttaaat                                                                          >  8:452188/1‑135 (MQ=255)
                                                               tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCAGTGCCTCTCTGTGGT‑CCCGGGCATCCAGACCGGG‑A‑CCCGGCCGGGGGCAAACGGTTACGTGCCACATTCAATGACAGTAACA‑ATAAAATGatat                                                                          >  4:828930/1‑140 (MQ=37)
                                                                ataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATGACCGGG‑A‑CCCGGACGGGGGGAAACGGTTCAGTGACCCATTCACAGACATTCACA‑ATAACATGATAtt                                                                         >  2:272674/1‑140 (MQ=255)
                                                                 taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCCTGCCCGGG‑G‑CCCGGGCGGGGGGGAAAGTTTAAGTGCCCCATTCACTGACCTTAACA‑ATAAAATTatat                                                                          >  6:514573/1‑138 (MQ=255)
                                                                     ctccAGCCCACACTTTAGGTCCCCTTCTTATATTTCTCCATGCCCTCTCTGTGTC‑CCGGGCCATCATGCCGGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATtcatca                                                                    <  4:632337/137‑1 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATTGACGGG‑C‑CACGGACGGGGGAAAACGTTTCAGTGACCCATTCACTGAAAATCACA‑ATAAAATTATATTCATCACg                                                                  >  7:839680/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACg                                                                  >  2:917338/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑C‑CCCGGTCGGGGGAAAACGTTTCAGTGCCCCATTCACTGTCATTCACA‑ATAACATGATTTTCATAACg                                                                  >  7:183471/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTACTCATGACCGGG‑G‑CCCGGGCGGGGGAAAACGGTTAAGAGCCACCTTCACGGTCCTTCACA‑ATAAAAAGATATTCATCACg                                                                  >  7:352890/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATGATGCCCGGG‑G‑CCCCGCCGGGGGGAAAACTTTAAGTGCCCCAGTCACTGACCTT‑ACACATAAAATGATATTAATAAcc                                                                  >  1:1007929/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATCATGACCGGG‑A‑CCCCGCCGGGGGGAAACGTTTAAGTGGCCCATTCACTGCCAATCAAA‑AAAAAATGATATTAATCAcc                                                                  >  1:822855/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGTCGGGGGTAAACGTTTGAGTGCCACATTAACAGACATTCACA‑ATAACATTATTTTAATAACg                                                                  >  3:924774/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑C‑CCCGGCCGGGGGGAAACCTTTCAGTGGCCCAATAACAGACATTAACA‑AAAACAAGATATTCATAAcc                                                                  >  3:530078/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGACTCTCTGTGGT‑CCCGGGGATCATTACCCGG‑C‑CCCGGGCGGGGGAAAACGGTTCAGGGCCCCATTCACTGAGCTTCACC‑AAAACATTATATTCATCACg                                                                  >  7:184373/1‑140 (MQ=255)
                                                                       gccccccACCCTTAAGGTGACATTCTTATAATACTCACTCGCCGCTCGGGGGC‑CCGGCTCAACA‑GCCCGGG‑CCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATtcatca                                                                    <  6:444051/133‑1 (MQ=255)
                                                                         cggCCACACTTATGGGCACCTTATTATAATTCGGAAGGGCCGCCCTGGGGC‑CCGGGCCATCATGCCCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGa                                                                <  2:1127722/139‑1 (MQ=255)
                                                                         aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTGATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACGGACATTCACA‑ATAACAAGATATTCATGACg                                                                  >  6:1027439/1‑138 (MQ=255)
                                                                         aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑A‑CCCGGCAGGGGGCCAAAGTTTCAGTGCCACATTGCATGACATTCACA‑ATACAATGGTATtcaaaccg                                                                  >  6:1030561/1‑133 (MQ=255)
                                                                           gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGCTGATGGACGGG‑T‑ACCGGTCGGGGGAAAACGTTTCAGTGGCCCCATAACTGTCAGTGACA‑ATAACAAGATATTCATAAccgt                                                                >  6:924372/1‑135 (MQ=255)
                                                                            ccACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATTACCCGG‑G‑CCCGGGCGGGGGAGAACGGTTCAGTGCCACCTTAACTGACAATAGCA‑ATAACAAGATATTCAACACGGAggg                                                             >  4:526402/1‑138 (MQ=255)
                                                                               cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCTACATGAACGGG‑A‑ACCGGACGGGGGGAAACGTTTAATTGGCACATTAACTGACATTCACA‑AAAACATGATATTAATAACGGAGTGAcg                                                          >  1:149790/1‑139 (MQ=255)
                                                                               cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑G‑CCCCGCCGGGGGAAAACGTTTAAGTGCCCCCTTCACTGACATTAACA‑ATAAAAAGATATTAATAACGGAGTGACt                                                          >  2:1174955/1‑140 (MQ=255)
                                                                               cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCCGG‑A‑CCCGGACGGGGGCAAACGGTTCAGTGCCACATTCACTGACCATAAAA‑ACAACCTGATATTCCTCACGGAGTGAcg                                                          >  2:310314/1‑139 (MQ=255)
                                                                               attttattgtcACTTTCTGATATTTGTCTATTCCCTCTCTGTGGG‑CCGGGACAACTTTCCTGTG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACt                                                          <  3:1060643/133‑1 (MQ=255)
                                                                                 cTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAAACATGACCGGG‑A‑CCCGGAGCGGGGAAAAAGTTTAAGTTCCAAATTAAATTAAATTCACA‑ATAAAAAGAAATTAAAAAAGGAGTGACt                                                          >  6:863699/1‑138 (MQ=255)
                                                                                     aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGGCAACA‑GACCGGC‑C‑CCCCGGCGGGGGGAAACGTTTAACGGGCACCATCACGGGCATTACCA‑AAAAAAAGATATTAATAACCGGGTGAGTATGttt                                                    >  7:201148/1‑139 (MQ=255)
                                                                                     aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCAAAATTACCGGG‑A‑CCCGGCCGGGGGCAAACGTTTCAGTGCCACATTCACAGAACTTCACA‑AATACATTATATTAAAAACGGAGTTAcgtagtga                                                    >  2:144876/1‑133 (MQ=255)
                                                                                     aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATAATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACCTTCACTGACATTCACA‑ATAACAATATATTCATCAACGAGTGACTATGTTa                                                    >  7:361668/1‑140 (MQ=255)
                                                                                         gACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCAACATGACCGGG‑C‑CCCGGACGGGGGAAAACGTTTAAGTGACACAATAAAGGACATTCACA‑ATAACATGAAATTAATCACGGAGGGACTATTGTACAGa                                                >  3:1039809/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGGAAACGTTTCAGTGCCACATTAACTGACATTCACA‑ATAACATGATATTCATCAAGGAGTTACTATGTTAAAGAt                                               >  2:281177/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGttt                                                                                                                 >  3:58127/1‑75 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGttt                                                                                                                 <  4:58127/75‑1 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACCTTAACTGACATTCACA‑ATAACATGATATTAATCACGGAGTGACTATGTTACAGAt                                               >  7:62403/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAATAACTGACATTCAAA‑ATAACATTATATTCAACACGGAGTTAATATGTTACAGAt                                               >  7:62408/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑C‑CCCCGCCGGGGGAAAACCTTTCCGTGCCCCATTAACTGCCATTCACA‑ATAAAAAGAATTTAATACCGGAGTGACTATGTTACACAt                                               >  2:443551/1‑140 (MQ=255)
                                                                                            cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGAACGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTAACTTACATTCACA‑AAAACATGATATTAATCACGGAGTGACTATTTTACAGAt                                               >  6:772581/1‑138 (MQ=255)
                                                                                            cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCACAATGAACCGG‑G‑CCCCGCCGGGGGCAAACGATTAAAGGCCCCATTCACTGACATTCCAA‑ATAACATGATATTAATCACCGAGTTGATAGTTTACAGAt                                               >  5:496403/1‑138 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGATCATGACCGGG‑C‑CCCGGCCGGGGGAAAAAGTTTACTTGCCACAATCCCGGACATTCACC‑ATAAAATTAAATTCATGACGGATTGACTATTTTAAAGATATTc                                           >  8:83165/1‑140 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑A‑CCCGGCCGGGGGTAAACGTTTCAGTGCCCCATTCAATGACATTGACA‑ATAACATGATATTCATCACGGAGTGACGATGTTACAGATAGtg                                           >  2:1107336/1‑139 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑C‑CCCCGGCGGGGGGAAACGTTTCAGTGCCCAAATAACAGCCATTCAAC‑ATAACATGATATTCATGACGGAGTGACAATGTTACAGATAGTc                                           >  3:1102525/1‑140 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTAACTGGT‑CCCGAACGTCAAGCCACGG‑A‑AACGGCCGGGGGAAAAAGTTTCAGTCCCACATAAACACACAAAAACA‑ATAACATCATATTAATGACGGAGTTACAATATTTAAGATAgaa                                           >  4:974918/1‑138 (MQ=255)
                                                                                                tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATAATTACCCGG‑A‑CCCGGTCGGGGGAAAACGTTTCACTGCCACATTCACTGACATTGACA‑ATAAAATGATATTAATCACTTTGTGACTATGTTAAAtatattt                                           >  6:683376/1‑135 (MQ=255)
                                                                                                     aTTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGCCGGGGGAAAACGGTTCAGTGCCACATTCACTGACATTCACC‑ATAACATGATATTCATAACGGAGTGACTATGTTACAGATAGTCGgcgcgc                                    >  4:875134/1‑140 (MQ=255)
                                                                                                      ttCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTAACAGACATTAACA‑ATAAAAAGATAATAAAAACGGAGTGACTATTTTAAAGATATTAGgcgcgca                                   >  2:1118143/1‑139 (MQ=255)
                                                                                                         tGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACCGGG‑A‑CCCCGGCGGGGGAAAACGTTTAAGTGGCACCTTCACTGACATTCACA‑ATAAAATTATATTAATAACGGAGGGACTATGTTAAAGATAGTCGGCGCGCTGAt                                >  1:742153/1‑140 (MQ=255)
                                                                                                         tGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑ACCGGGCGGGGGAAAACGTTTCAGTGCCACATTCACGGACATTAACA‑ATAACATGATTTTCATAACGGAGTGACTATGTTACAGATAGTCGGCGGGCTGAt                                >  4:115276/1‑140 (MQ=255)
                                                                                                         tGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGTCCGGG‑A‑CCCGGCCGGGGGGAAACGTTTGAGTGCCACATTCACTGACATTCACA‑ATAACATGAATTTCCTCACGGAGTGAGTATGTTACAGCTATTCGGGCCGCTGAt                                >  4:276731/1‑140 (MQ=255)
                                                                                                                     ctGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGGTTAAGTGCCCCAATCACAGACAATCACA‑ATAACAATATATTCATCACGGAGTGACTATGTTTCAGATAGTCGGGGCGCGGATTATGGTGATCgg                    >  2:59698/1‑139 (MQ=255)
                                                                                                                       gtgGT‑CCCGGTCATCATGACCGGG‑G‑CCCGGACGGGGGAAAACGTTTAAGTGGCACATTCACTGACATTCACA‑AAAACATGATATTAATCACGGAGTGACGTTGTGACAGATAGTCGGCGCGCGGATTCTGCGTATCGc                    >  6:80508/1‑138 (MQ=255)
                                                                                                                        gggT‑CCGGGCCATCA‑GCCCGGGCA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCa                   <  5:639837/137‑1 (MQ=255)
                                                                                                                          gT‑CCCCGTCCTCATGACCGGG‑C‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAATCACA‑ATAACATGATAATAATCACGGAGTGGCTATGTTACAGATAGTTGGCGCGCTGATTATGCAGTTCGCAGGAt               >  8:136933/1‑140 (MQ=255)
                                                                                                                           t‑CCCGGGCCTAATGACCGGG‑C‑CCCGGCCGGGGGGAAACGTTTCAGTGCCACATTCACAGACATTAACC‑AATACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCGGCTTAAGCGGGTCGCAGGGtt              >  4:312221/1‑140 (MQ=255)
                                                                                                                             ccccGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTGACTGACATTAACA‑ATAACATGATATTCATCACGGAGTTAATATGTTAAAGATAGTAGGCGCGCTGATTATGCAGATCGCAGGAt               >  6:691698/1‑138 (MQ=255)
                                                                                                                               gggTCATCAGCACGGGG‑A‑CCCGGACGGGGGAAATCGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGc           <  8:535147/139‑1 (MQ=255)
                                                                                                                                      catGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGAGGATCGCAGGCTTTGCCAttctt    >  8:808281/1‑140 (MQ=255)
                                                                                                                                        tGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCg  >  1:475665/1‑140 (MQ=255)
                                                                                                                                        tGACCGGG‑A‑CCCGGACGGGGGAAAACGGTTCAGGGCCCCAATCACTGACATTCACA‑AAAACATGATATTCAGCCCCGAGTGACTATGTTACAGATAGTCGGCGCGCTGAATCTGCTGATCGCAGGATTTGCCTtactt    >  5:47270/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                            
AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCG  >  NZ_CP022686/4677904‑4678178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A