Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F35 I0 R1	304	9232.4	10623078	98.3%	10442485	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,678,041	A→C	21.6%	intergenic (+39/‑82)	agn43 → / → CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,678,041	0	A	C	21.6%	65.4 / 12.9	103	intergenic (+39/‑82)	agn43/CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (66/4); new base C (9/12); total (87/16)
Fisher's exact test for biased strand distribution p-value = 1.08e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 105 total aligned reads displayed.

CCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCC  >  NZ_CP022686/4677911‑4678180

cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCAACATTC‑CCGGGAccc                                                                                                                                       >  2:1154641/1‑140 (MQ=255)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCC‑CGGCAACAAGa‑cccggaccc‑ggccc                                                                                                                                 >  1:923440/1‑137 (MQ=21)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCCTCATGC‑CCGGGACCC‑GGGC‑g                                                                                                                                >  1:468855/1‑140 (MQ=255)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGGccccgccc                                                                                                                                  >  8:349662/1‑135 (MQ=255)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑g                                                                                                                                >  8:107768/1‑140 (MQ=255)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCCGGCCCC‑GGAC‑g                                                                                                                                >  5:767954/1‑140 (MQ=255)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGGATCATTA‑CCGGGGccccgccg                                                                                                                                  >  1:282/1‑135 (MQ=21)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCAAGG‑CCCGGACCC‑ggccc                                                                                                                                 >  5:420851/1‑137 (MQ=21)

     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCAA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCATGA‑CCGGGACCC‑ggg                                                                                                                                   >  4:828113/1‑137 (MQ=255)

       cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCAGCATGA‑CCGGGACCC‑GGAC‑g                                                                                                                                >  4:502947/1‑138 (MQ=255)

       cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTAATCATGA‑CCGGGACCC‑GGAC‑g                                                                                                                                >  3:120427/1‑138 (MQ=255)

          tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATAATGA‑CCGGGACCC‑CGCC‑g‑ggggt                                                                                                                          >  6:1247348/1‑139 (MQ=255)

          tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGCAACATTA‑CCCGGCCCC‑GGGC‑g‑ggggg                                                                                                                          >  7:1182692/1‑139 (MQ=21)

           cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTGATCATGA‑CCGGGACCC‑AGGC‑G‑GGGGaa                                                                                                                         >  7:155875/1‑140 (MQ=255)

           cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTGATAATGA‑CCGGGGCCC‑GGCC‑G‑GGGGaa                                                                                                                         >  5:210844/1‑140 (MQ=255)

           cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGCATCATGA‑CCCGGACCC‑GGAC‑G‑GGGGaa                                                                                                                         >  2:645082/1‑140 (MQ=255)

           cAGGCCGGTTATGCCCACAGCATCAACGGCAGAAGCGCTGAAGGTGATAACAGTCAGGCCA‑CACTGAATGTGACCTTCGGATAATTCTGCATTGCCTCTCTGTGGT‑CAC‑CGTCAAGAATA‑CCCGGCCCC‑CGTC‑g‑gggggt                                                                                                                         >  1:1191960/1‑138 (MQ=21)

             ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGGATCATGT‑CCGGGTCCC‑GGAg‑g‑ggggca                                                                                                                         >  4:167826/1‑136 (MQ=25)

                  tttATCCCCACCTCATCAACGGCAGCGGCGGTGATGTTTATAACCGCCCGCCCA‑CACTTATGGTCCCCTTCTTATATTTGTGAATTGCCTCTCTGTGGC‑CCC‑GGCAATCTTCC‑CGGGGACCC‑GGAC‑G‑GGGGAAAACGt                                                                                                                    <  4:331755/137‑1 (MQ=255)

                          cacaGCATCAACGGCAGCAGCGCTGAAGGATATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGAAAATTCTGCATTGCCTCTCTGTCGT‑CCC‑CCGGAGGAAGA‑CCGGGACAC‑GGGC‑C‑GGGGATAACGTTTCAGTgg                                                                                                            >  4:186649/1‑137 (MQ=21)

                            caGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCAGCATGA‑CCCGGACCC‑CCAC‑GCGGGGAAAACG‑TTAAGTGCcaca                                                                                                        >  1:461875/1‑140 (MQ=25)

                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACGTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATAATGACCCGGGACCC‑‑GAC‑G‑GGGGTAAACCGTTAAGTGGcacaa                                                                                                       >  8:382867/1‑139 (MQ=25)

                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTAATCATGT‑CCCGGACCC‑CGCC‑G‑GGGGAAAACGTTTAAGTGCcccaa                                                                                                       >  6:1078482/1‑139 (MQ=37)

                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCAAACAGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTTCcccaa                                                                                                       >  6:135739/1‑139 (MQ=37)

                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTATGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCATGA‑CCGGGCCCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCcacca                                                                                                       >  2:165905/1‑138 (MQ=255)

                               cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGGATCATGA‑CCGGGGCCC‑GGGC‑G‑GGGGGAAACGTTTAAGTggcccaa                                                                                                       >  3:663824/1‑132 (MQ=25)

                                 tCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGGATAATTC‑CCCGGCCCC‑CGAC‑G‑GGGGTAAACCGTTAAGGGCcaacatcaa                                                                                                   >  6:1061931/1‑133 (MQ=21)

                                  cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCAACATGA‑CCGGGACCC‑GGCC‑G‑GGGGAAAACGTTTAAGTGCCCCATTCACt                                                                                                  >  5:527275/1‑140 (MQ=255)

                                   aaCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGCC‑G‑GGGGAAAACGTTTCAGTGCCACATTCAc                                                                                                   >  4:471715/1‑138 (MQ=255)

                                   aaCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGGATCATGG‑CCGGGACCC‑GGGC‑G‑GGGGGAAAAGGTTAACTGCCCCAttgaa                                                                                                   >  3:1343979/1‑135 (MQ=25)

                                     cGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCAACATGA‑CCCGGACCC‑GGAC‑G‑GGGGAAAACGGTTAAGTGCCACATTCACAGAc                                                                                               >  7:92435/1‑140 (MQ=37)

                                       gcagcaGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGCCTCATGA‑CCGGGCCCC‑GGAC‑C‑GGGGGAAAAGTTTCAGGGGCACCTTCACTGACAt                                                                                             >  1:284820/1‑140 (MQ=25)

                                        cagccgCGCTGAGTGTATTAACAGCCCGCCAA‑CACTCAATGTCCCCTTTTTATAATTCTGCATTGCCGCTTTGGGGT‑CCG‑GGGCATATTCC‑CGGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACa                                                                                              <  4:287616/138‑1 (MQ=255)

                                        cagcagCGCTGAAGGTTATAACAGTCAGTCCA‑CACTGAATGTGTCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACAtt                                                                                            <  8:548496/140‑1 (MQ=255)

                                         agcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTGCTCATGA‑CCGGGGCCC‑GGGC‑G‑GGGGGAAACGTTTCAGTGGGACATTAACTGACAtta                                                                                           >  5:712681/1‑139 (MQ=37)

                                         agcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGC‑ACGGGCACC‑CGAC‑G‑TGGGAAACCGTTTCAGTGCCAAATTCACTGACAtta                                                                                           >  2:104897/1‑139 (MQ=37)

                                         agcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCCTTGCCACATTCACTGACAt                                                                                             >  4:489134/1‑138 (MQ=255)

                                         agcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAA‑G‑GGGGAAAACGTTTCAGTGCCACAATAACTGACaata                                                                                           >  1:1029383/1‑137 (MQ=255)

                                         agcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCCTCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCCCAGTCCCTGAcct                                                                                             >  4:1293725/1‑136 (MQ=37)

                                         agcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCATGA‑CCGGGACCC‑CGTC‑G‑GGGGAAAACGGTTCACTGCCACATTCACCGACATTc                                                                                           >  5:1180787/1‑140 (MQ=37)

                                          gcagcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCATGA‑CCGGGACCC‑GGCC‑G‑GGGGAAAACCTTTAAGTGCCACATTAACTGACAtt                                                                                            >  3:528797/1‑138 (MQ=38)

                                            agcGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCAAGA‑CCGGGCCCC‑GGAC‑G‑GGGGGAAACGTTTCAGTGCCCCAATGACTGACATTca                                                                                          >  3:335701/1‑138 (MQ=37)

                                             gcgcTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATAAATA‑CCGGGTCCC‑CGCA‑G‑GGGGTAAAAGTTTCAGTGGCAAATTCCCTGACATTCCCaa                                                                                       >  1:278143/1‑140 (MQ=255)

                                                  gttttttATAACACTCCGGCAA‑CACTTATTGTGACCTTATTATAATACTGCTTGCCCTTTCGGTGGG‑CCC‑GCTCATCATGC‑CGGGCACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACa                                                                                  <  6:1025890/135‑1 (MQ=255)

                                                     ggTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCATGA‑CCGGGGCCC‑GGCC‑G‑GGGGAAAACGTTTCAGTGCCCCATTCACTGCCATTCACAATAACaa                                                                                 >  3:1256538/1‑137 (MQ=38)

                                                        tataACAGTCCGGCCA‑CACTGAATGTGACCTTCTTATATTTCTGCATTGCCTCTCTGTGGT‑CCG‑GGTCATCATGC‑CGGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGat                                                                              <  4:967471/138‑1 (MQ=255)

                                                        tataACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTGAACATGT‑CCGGGCCCC‑GGAC‑G‑GGGGAAAAAGTTTAAGTGGCACATTAACAGACCTTCACAATAACAAGatat                                                                            >  5:1047085/1‑140 (MQ=37)

                                                          taACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCC‑GGGGCTCAAGG‑CCGGGGCCC‑CGGC‑G‑GGGGTTAACGTTTGAGGGGCACATTCACGGACAGTGACAATAAAATGATAtta                                                                          >  1:81093/1‑139 (MQ=255)

                                                          taACAGTCAGGCCA‑CACTGAATGTGACCTTCTGAAAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGGATCATTA‑CCGGGGCCC‑GGAC‑C‑GGGGGAAACGTTTCAGTGCCCCATTAACAGACAATCAAAAAAACAatatat                                                                            >  3:1081963/1‑138 (MQ=255)

                                                              agtccccccA‑CACTCAATGTCACCTTCTTATAATTCTCCATTGCCTGTGGGTGGC‑CCC‑GTCCAACATCA‑CGGGTACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATtcatca                                                                      <  8:510560/133‑1 (MQ=255)

                                                              aGTCCGCCCA‑CACTTAATGTTACATCCTCATATTTCTCAATGCCCTCTCTGTGGA‑CCC‑GGTCACCATGC‑CGGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATtcat                                                                        <  4:493601/138‑1 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGGATGGCCTCTCTGTGGT‑CCC‑GGTCATCATGC‑CCGGGGCCC‑GGCG‑G‑GGGGTAAACGTTTCATTG‑CAGTTTAACTGTAATTCACAATAAACAGATATTCATCGCgg                                                                   >  8:1125495/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTGATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACAGACATTCACAATAACATGATATTAATCACg                                                                    >  8:586168/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTAAGTGCCACATTCACTGACATTCACAATAAAATGATATTAATCACg                                                                    >  2:133549/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGGTTCAGTGCCACATTCACTGACATTAACAATAACATGATATTAATAACg                                                                    >  2:1029180/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTAATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCACTTCCACATTCACTTACATTCACAATAACAAGATATTCATAAcc                                                                    >  2:132005/1‑139 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGGAGCATGG‑CCGGGCCCC‑GGCC‑G‑GGGGGAAACGTTTAAGTGGCAAATTGACAGACAGTCACAATAAAATGATATTAATAACg                                                                    >  8:1092108/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCATGA‑CCGGGACCC‑GGCC‑G‑GGGGTAAACGTTTCAGTGCCACCTTCACTGACATTAACAATAACATGATATTCATCCCg                                                                    >  7:618800/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCAAGA‑CCGGGGCCC‑GGCC‑G‑GGGGAAAACGTTTCATGGCCCCAATAAAGGGCATTCACAATAACATTATATtcatgaag                                                                    >  7:562121/1‑136 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGGATCATGT‑CCGGGGCCC‑GGCC‑G‑GGGGGAAACGGTTCTGTGGCCCCTTGACGGACATTGACAATTAAATTATATtgataaag                                                                    >  1:865308/1‑136 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGGATCAAGA‑CCCGGGCCC‑CGCC‑G‑GGGGGAAACGTTTAAGTGGCCCCTTCACTGCCATTCACAATAACATTATATTAATCACg                                                                    >  7:137370/1‑140 (MQ=255)

                                                                tCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGCATCATGA‑CCGGGGCCC‑GGCC‑G‑GGGGGAAACGGTTAAGTGCCCCATTCACATACATTCAAAATAACAAGATATTAATAAcc                                                                    >  1:1230030/1‑139 (MQ=255)

                                                                 cAGGCCACCACTTAAGGTGACCTTCTGATATTCCTCAATTCCCTCCCGGTGGA‑CCG‑GGTCACCATCC‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACg                                                                    <  6:531322/140‑1 (MQ=255)

                                                                 cAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCAAGA‑CCGGGCCCC‑GGGC‑G‑GGGGGAAACGTTTCAGTGCCACATTCACAGCCATTCCCAATAACATGTGATTAATCACgg                                                                   >  8:623228/1‑140 (MQ=255)

                                                                  aGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCATGA‑CCGGGACCC‑GGGC‑C‑GGGGGAAACGTTTAAGTGGCACATTCACAGACATTAACAATAACAATATATtcaaaaac                                                                    >  4:1353272/1‑133 (MQ=255)

                                                                  aGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATAATTA‑CCGGGGCCC‑CGGC‑G‑GGGGGAAACGTTTAAGTGCCCCAATCACTGACATTAAACATAAAAAGATATtcaacaag                                                                    >  4:660821/1‑136 (MQ=255)

                                                                    gCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGGATCAGGC‑CCGGGGCCC‑GGGC‑G‑GGGGCAAACGGTTCAGGGGCCCATTCACTGACATTCACAATAACATGATATTGATCACggt                                                                  >  3:1208058/1‑137 (MQ=255)

                                                                    gCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCAACATGA‑CCGGGCGCC‑GGAC‑G‑GGGGAAAACGTTTGAGTGCCACATTCACTGACATTAACAATAACATGAAATTCATAACGGatt                                                                >  8:425794/1‑138 (MQ=255)

                                                                    ccaa‑cacTGATTGTCACCTTCTTATATTAGGGAATTGCCTCTC‑GTGGC‑CCCGGGCCCCCAGGC‑CCGGGCCCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGt                                                                <  5:976606/137‑1 (MQ=255)

                                                                      ca‑caGTTAATTTTACCTTCTCATAATACTCCATTGCCGCTCGGTGTC‑CCC‑GTTCATCATGC‑CGGGCACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                              <  2:833879/140‑1 (MQ=255)

                                                                           cTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCATGC‑CCGGGCCCC‑GGGC‑G‑GGGGTAAACGTTTCAGTGCCACATTACGAGACCTTCACAATAACAAGATATTCATCACGGAGGGACTAt                                                          >  2:247287/1‑140 (MQ=255)

                                                                             taaTTTGACATTCTTAAAATTCTGCATTGCCTCTCTGGGGC‑CCC‑GGCCATCATCC‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGt                                                        <  2:614385/139‑1 (MQ=255)

                                                                               aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTGATCATGA‑CCGGGGCCC‑CGCC‑G‑GGGGGAAACGTTTAAGGGCCACATTAACGGACAATAACAAAAACAATATATTCATCACGGAGTGTCTAttttt                                                      >  1:387304/1‑139 (MQ=255)

                                                                               aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGGCATCATGA‑CCGGGGCCC‑GGAC‑G‑GGGGGAAAACTTTAAGTGGCCCATTCACAGACATTGACAAAAACAGTATAATAATAACCGAGTGACTATGttt                                                      >  1:722806/1‑139 (MQ=255)

                                                                                 gtgACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CAC‑GGTGATCATGA‑CCGGGTCCC‑GGCC‑A‑GGGGTTAACCTTTCAGTGCCACAATAAAAGCAATTGACAATAACATGAAATAAATCACCGAGTGACTATCTTa                                                      >  4:135451/1‑138 (MQ=255)

                                                                                    aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGT‑CCGGGTCCC‑GGGC‑G‑GGGGAAAACGTTTCACTGGCCCAATCACTGAACTTCACAATAAAATCCTATTCATCACCGAGTGGCTAATTTACAGaa                                                 >  7:960120/1‑139 (MQ=255)

                                                                                    aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCCGGACCC‑GGCC‑G‑GGGGAAAAACGTTAAGTGGCACATTCACAGACATAGACAAAAAAAAGATAATAATAACGGAGTGACTATTTTAAAGaa                                                 >  1:553755/1‑139 (MQ=255)

                                                                                    aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTGATAATTA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACCTTTAAGTGCCACAATAACTGACATTCACAATAACATTATATTAATAACGGAGTGACTATGTTAAAGaa                                                 >  2:140020/1‑139 (MQ=255)

                                                                                        tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGGCCC‑GGCC‑G‑GGGGAAAACGTTTAAGTGCCACATTCACGGCAATTAACAATAAAATGATATTAATCCCGGATTGACTATTTTACAGATatta                                             >  6:349460/1‑137 (MQ=255)

                                                                                        tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATAATGA‑ACGGGACCC‑GGAC‑G‑GGGGAAAAAGTTTAAGTGGCACAATCACTGACCATCACAAAAACATGATATTCATAAACGAGTTACTATGTTACACATAGtg                                             >  1:324810/1‑139 (MQ=255)

                                                                                        tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑CGTCATCATGA‑ACGGGACCC‑GGAC‑G‑GGGGAAAACTTTTCATTTCCACATTAAAAGACAATCACAATAAAATGATAATCATCACGGAGTGACAATGTTACAtatatta                                             >  1:788977/1‑137 (MQ=255)

                                                                                          tGATAATTCTGCATTGCCTCTCTGTGTT‑CCC‑GGTGATCAAGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACCTTTCAGTGCCACATTGACTGACAATCTCAATAACATGATATACAACACTGAGTTACAATTTTACAGATatgagg                                           >  8:85372/1‑135 (MQ=255)

                                                                                          tGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑ACCGGACCC‑GGGC‑G‑GGGGGAAACGTTTAAGTGCCACATTACCTGCCATTCAAAATAAAATGATTTTAATAAAGGAGTGACTATGTTTAAGATAGTc                                             >  4:1348054/1‑138 (MQ=255)

                                                                                          tGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGGCATCATGT‑CCGGGGCCC‑GGGC‑G‑GGGGAAAAAGTTTCAGTGCCACAATCACTGACATTCACAATAACATGATATTCATCACGGAGTGCCTATGTTACAGATAGTc                                             >  4:61494/1‑138 (MQ=255)

                                                                                              aaTTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCCTCATTA‑CCGGGACCC‑GGAC‑G‑GGGGGAAACGTTTCAGTGCCCCATTCAATGACATTCACAATAACATGATATTAATCACGGAGTGACTATGTTTAAGATAGTAGgcg                                         >  4:1272631/1‑138 (MQ=255)

                                                                                                 tCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGC‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                            <  5:573211/116‑1 (MQ=255)

                                                                                                 tCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                            >  6:573211/1‑116 (MQ=255)

                                                                                                   tGCATTGCCTCTCTGTGGT‑CCC‑GGTCCTCATGA‑CCGGGCCCC‑GGACGG‑GGGGAAAACGTTGCGGGGCACCATGC‑CTGTCATTCCCAATAACATTATAATCATAACCGAGTGCCAATGTTACAGCTAGTGcgcgcggggtt                                  >  2:964586/1‑135 (MQ=255)

                                                                                                   tGCATTGCCTCTCTGTGGT‑CCC‑CGTACTCATGA‑ACGGGACCC‑GGGC‑G‑GGGGGAAACCTTTGAGTGCAAAAATGACTGACATTGACAATAACATTATATTCATCACCGAGTGACTATGTTACACTAAGTCGGCGCGCAGAt                                  >  6:1163957/1‑140 (MQ=255)

                                                                                                    gCATTGCCTCTCTGTGGTCCCC‑GGGCA‑AAAGT‑CCGGGGGAC‑CGCC‑G‑GGGGAAAACGTTTAAGGGGCACAATCAAAGAAATTAACAAAAAAAATATATTAATCACGGCGTGTCTATTTTTAAGATAGTAGgggcacaggat                                 >  8:569710/1‑133 (MQ=255)

                                                                                                     cATTGCCTCTCTGTGGT‑CCC‑CGTCATCATGT‑CCGGGACCCGGGGC‑G‑GGGGAAAA‑GTTTGAGTGCCACAATCACTGACATTCACAAAAACATGAAATTAATAAAGGAGTGACTATGTTTCACATAGTCCgcgcgcagct                                  >  4:197277/1‑134 (MQ=255)

                                                                                                            tctctGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTAACTGCCATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctgctg                           >  4:1022624/1‑138 (MQ=255)

                                                                                                              agttgggT‑CCG‑GCTCCTCAGGA‑CCGGTACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGAt                         <  4:174115/133‑1 (MQ=255)

                                                                                                                        cc‑GGGCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCCCATTCACTGACATTCACAATAACATGATATTAATAACGGAGGGACGATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTg              >  7:920369/1‑140 (MQ=255)

                                                                                                                        cc‑GGGCAACAAGA‑CCGGGACCC‑GGAC‑G‑GGGGTAAACCGTTCAGTGGCACATTAACTGCCAATAACAATAACAAGATAATAAACACGGAGTGTCTATGGTACAAATAGTAGGCGCGCAGATTATGCTGATCGCAGGAtttt              >  2:950547/1‑139 (MQ=255)

                                                                                                                         cccgTCATCAATC‑CCCGGACCC‑GGTC‑G‑GGGGAAAACGTTTAAGTGCCCCCTTAACTGACCATCACAATAACATGATATTAATAACGGAGTGGCTAAGTTTAAGATAGTCGGCGCGCTGATTAGTCTTATCGGAGGttttt              >  6:1299274/4‑139 (MQ=255)

                                                                                                                                  ttGA‑CGGGTACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct       <  4:1042332/137‑1 (MQ=255)

                                                                                                                                   tGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACTGATAGTCGGCGCGATGATTCTGCTGATAGCAGGATTTGCCATTCTTCg    >  5:1193858/1‑140 (MQ=255)

                                                                                                                                   tGA‑CCGGGACAC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGAGCGCTGATTCTGCTGATCGCAGGATTTGCAAgtctt      >  4:480281/1‑138 (MQ=255)

                                                                                                                                     a‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGCTCGCAGGATTTGCCATTCTTCGcc  >  1:341466/1‑140 (MQ=255)

CCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCA‑CACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCC‑GGTCATCATGA‑CCGGGACCC‑GGAC‑G‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCC  >  NZ_CP022686/4677911‑4678180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A