Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,504,324 C→A 17.2% P254H (CCT→CAT)  CIW80_RS07520 → YgcG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,504,3240CA17.2% 160.9 / 3.0 112P254H (CCT→CAT) CIW80_RS07520YgcG family protein
Reads supporting (aligned to +/- strand):  ref base C (43/45);  new base A (4/17);  total (47/65)
Fisher's exact test for biased strand distribution p-value = 1.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TACACCTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGT  >  NZ_CP022686/1504187‑1504463
                                                                                                                                          |                                                                                                                                            
tACACCTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTAGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTa                                                                                                                                            >  8:132882/1‑140 (MQ=255)
tACACCTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTGTGGTTTAGTTTATTTTTAGCAGCAATAAGCTGGCCTCGTCTCGCCGTACTTTTTAATATGTGCCCC‑GTAATTCCTa                                                                                                                                            >  6:10726/1‑140 (MQ=255)
tACACCAACAGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCCGGTGTCGGTCGTTTGTTTTACTTTATTTTTCTCCTCAATCTCCTGGCCGCGTCTCGCAGTACTTTTTAATATGTTCCGC‑GGATTTCCtt                                                                                                                                            >  4:220817/1‑139 (MQ=255)
 acacCTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTAGCAGCAATCTGCTGGCAGCGGCTCGCAGTAGTTTTTAATATGTGCCGC‑GGATTTACttt                                                                                                                                           >  2:717211/1‑138 (MQ=255)
  cacCTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCTTGTGTTTTAGTTTTTTTTTAGCAACCATATCCTGGGAGCGGCTCTCAGTAGTTTTTAATATGTGGCGC‑GGATTTGCTatt                                                                                                                                          >  1:774409/1‑139 (MQ=255)
     cTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTatgatg                                                                                                                                       <  5:108900/140‑1 (MQ=255)
        cGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTTGTTTATTTTTAGCCGCAAGCTGCTGGCAGCGGGTGGCAGGAGTTTTTAATATGTGCCGG‑GTATTTGCGATGctgctg                                                                                                                                    >  5:536667/1‑140 (MQ=255)
        cGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTATTTTTTTTTTAGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATTTTCCGC‑GTATTTACTATTATTCTg                                                                                                                                    >  3:801937/1‑140 (MQ=255)
        cGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTGTGTTTTTGTTTATTTTTAGCAGCCATCTGCGGGCAGCGTCGACCAGGAGTTTTTAATATTTTGCGC‑GTATTTTCTatgatgatg                                                                                                                                    >  1:387411/1‑140 (MQ=255)
         ggTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTTGTTTATTTTTCGCAGCAATATGATGGCAGCGGCGCGCAGTAGTTTTTAATATGTGCCGC‑GTTTTTCCAATTATTCTgg                                                                                                                                   >  4:271688/1‑140 (MQ=255)
          gTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATTATGCTGGc                                                                                                                                  >  4:189330/1‑140 (MQ=255)
             tGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTTTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTAGCAGTAGTTTTTAATATGTGCCGC‑GTATTTGCTATTATGCTGGcccc                                                                                                                               >  4:744132/1‑140 (MQ=255)
             tGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTATTTTTTTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGGAGTTTTTTATATTTGCAGC‑GTATTTCCTATTATTCTGGcccc                                                                                                                               >  1:736518/1‑140 (MQ=255)
             tGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTATTTTTTTTTTAGCAGCAAAAAGCTTGCAGCGTCTAGCAGTAGTTTTTAATATTTTCCGC‑GTATTTAATATGATTCTTGcccc                                                                                                                               >  7:412941/1‑140 (MQ=255)
             tGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTGTGTTTTATTTTATTTTTAGCAGAAATCTTCTTTCAGCGTATCGCAGATTTTTTTAATATTTTACGC‑CATTTTGCAATGATGCTGGcccc                                                                                                                               >  5:848590/1‑140 (MQ=255)
               gTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTAGCAGCAATCTGCAGGCAGCGGCTCCCAGTAGTTTTTAATATGTTCCGC‑GTATTTAAGATGATGCTGGACCCaa                                                                                                                             >  4:372349/1‑140 (MQ=255)
                     cTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCTCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATTTGCCGC‑GTATTTACTATTAATCTGGCCCCCAAAAATa                                                                                                                       >  3:756711/1‑140 (MQ=255)
                       gCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATaaa                                                                                                                     <  5:187126/140‑1 (MQ=255)
                               gACGATGTCATCATTCGGCGGGTGTCGGTCGTTTTTTTTAGTTTAATTTTAGAAGCAAAATGCTGTCAGCCTCTCGCAGTATTTTTTAATATGTGCCGA‑GTATATACTAAGATGATGGCCCAAAAAAATAAAAC‑CGCaaa                                                                                                            >  4:674935/1‑140 (MQ=255)
                                         tcatTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGaaaaaa                                                                                                  <  3:185272/140‑1 (MQ=255)
                                         tcatTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGaaaaaa                                                                                                  <  4:385588/140‑1 (MQ=255)
                                               ggcggGTGTCGGTCGTTTGTTTTAGTTTATTTTTAGCAGCAATATGCTGGCAGCCTCTCGCAGTAGGTTTTAATATTTTCCGC‑GTATTTACTATGAAGCTTGCCCCAAAAAAAAAAAA‑CTCTAAATTGAAAAAACaaaaa                                                                                            >  5:223495/1‑139 (MQ=255)
                                                gcggGTGTCGGTCGTTTGTTTTTTTTTTATTTTAGCCGCAAACTGGTGGCAGCCTCTAGCACTAGTTTTTAATATTTGGCGC‑CTATTTGCTATTATTGTGGGCCCCAAAAAAAAAAA‑CCCAAAAATTAaaaaaaaaaaaa                                                                                           >  7:1058/1‑138 (MQ=255)
                                                   ggggtcgggggTTTGTTTTTGTTTATTTTTCCCATCTATCTGTTGGCAGCGGCGTGCATATTTTTTTATTTTGTGCCGA‑GTATTACCTCTGATTCTGGCCCCAAAAAATAAAAA‑CGCTACAGGTAAAAAAAAAAcgaaga                                                                                        <  8:579970/137‑1 (MQ=255)
                                                   ggTGTCGGTCGTTTGTTTTAGTTTTTTTATCGCTGAAATCTGCTGGACGGGTCTCGCATTATTTTTTAATTTGTGCCGC‑GTATTTCTTTTTAGGGTGGCCCCAAAATAAAAAAA‑CGATAAAGTCAAAAAACAAAacaaga                                                                                        <  2:285930/140‑1 (MQ=255)
                                                     tgtCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACt                                                                                      >  6:536226/1‑140 (MQ=255)
                                                       tcggtcgTGTGTTTTATTTTATTTTTAGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCATATTATGCTGGCCCCAAAAAATAAAAA‑AGCTATATTTAAAAAACAAAAGAAGACTcc                                                                                    >  2:820308/1‑140 (MQ=255)
                                                           tcgTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTAGCAGTAGTTTTTAATATGTGCCGC‑GTATTTACTATGATGCTGGCCCCAAAAAATAAAAA‑CACTAAAGTGAAAAAACAAAAGAAGACTCCGCaa                                                                                >  6:374318/1‑140 (MQ=255)
                                                              tgtgtttTATTTTTTTTTTCGCAGCAATATTCAGGCAGCGTCTCACATTATTTTTTAAAATGTGACGC‑TGTTTTACTATGATGCTGGCCCCAAAAAATAAAAA‑AGAGAAAGTGATAAAAAAAAAGAAGACAACGCAAttt                                                                             >  2:292085/3‑140 (MQ=255)
                                                                     tAGTTTTTTTTTCGCAGCAATCTGCTGGCTGCGTATCGCATTAGTTTTTAATTTGT‑CCGCGGTATTTCATATGAGGCTGGCCCCAAAAAAAAAAAA‑CGCTAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAAt                                                                      <  7:56639/140‑1 (MQ=255)
                                                                       ttttATTTTTCGCACCAATGTGCGGGCTGCTTCTCGCATTTGTTTTTAATATGGGCCGC‑TTATTTCCTATGATGCTGCCCCCAAAAAATAAAAA‑CGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCa                                                                    <  8:412941/139‑1 (MQ=255)
                                                                       gTTTTTTTTTGGCAGATCTCTGTTGGCTGCGTCTCTCTTTATGTTTAATTATGTGCGGC‑GTATTACCTATTATCCTGGCCCCAAAAAATAAAAA‑CGCAAATGTTAAAAAAAAAAACAAGACTCCGCAATTTCGTTAATCa                                                                    <  4:279499/140‑1 (MQ=255)
                                                                        tttATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAg                                                                   >  2:777681/1‑140 (MQ=255)
                                                                           ttttttCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGAc                                                                <  8:574812/139‑1 (MQ=255)
                                                                           aTTTTTCGCAGCAATCGGCTGGCAGCGTCCCGCATTTGTTTTTAATATGGGCCGC‑GTATTTCCTATGATGCTGGCCCAAAAAAATAAAAA‑CGCTAAATTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGAc                                                                <  1:213781/140‑1 (MQ=255)
                                                                            tttttCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCAGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTAGTGAATCAGGACg                                                               >  7:885365/1‑140 (MQ=255)
                                                                                 cGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTATAAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTACGTGAATCAGGACGAAAtt                                                          >  5:926224/1‑140 (MQ=255)
                                                                                        aTCTGCTGGCAGGGTCTCTCTGTAGTTTTTATTATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAC‑CGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCAttgtt                                                   <  1:559663/140‑1 (MQ=255)
                                                                                            gctggctgGGTCTCGCTGTAGTTTTTAATATGTGCCGC‑GTTTTTCTTATGATGCTGGCCCCAAAAAAAAAAAC‑CGCTAAAGTCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTc                                               <  1:337907/140‑1 (MQ=255)
                                                                                             ctgGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCg                                              >  5:153960/1‑140 (MQ=255)
                                                                                               ggCTGCGTCTCGAATTTGTTTTTAATATGGGCCGG‑GTTTTACATATCTGGCGGCCCCAAAAAAAAAAAAA‑CTAAAAAGTTAAAAAACAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTg                                            <  1:512338/140‑1 (MQ=255)
                                                                                                gCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTAGTgt                                           >  6:620704/1‑139 (MQ=255)
                                                                                                  cgCGTCTCGCAGTAGTTTTTAATAGGCGCCGC‑GTTTTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTTAAGTTAAAAAAAAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                         <  7:831532/139‑1 (MQ=255)
                                                                                                  atcgactcGCAGTCGTTTTTAATCGGTGCCTC‑TTATTGCCCATGCTGCGGCCCCCAAAAAAAAAAAC‑CGCCAACGTCAAAAAAAAACCGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                         <  4:505937/135‑1 (MQ=255)
                                                                                                  aGCGTCTCGCAGTAGTTTTTAATATGTGCCGG‑TTATTTCCTATGATGCTGGCCCAAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                         <  2:791504/140‑1 (MQ=255)
                                                                                                  aGCGTCGCGCAGTATTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                         <  8:529008/140‑1 (MQ=255)
                                                                                                    ggTCTCGCTTTAGTTTTAAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCAAAAAAAAAAAAA‑CGCTAAAGTTAAAAAACAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                       <  3:848089/139‑1 (MQ=255)
                                                                                                    cggctcGCATTAGTTTTTAATAGGTGCCGG‑GTATTACCTATGATGCTGGCCCCAAAAAATAAAAC‑CGCTAAAGCGAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                       <  5:568345/137‑1 (MQ=255)
                                                                                                    cGTCTCGCTGTAGTTTTTAATATGTGCCGC‑TTATTTCCTATGATGCTGGCCCAAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                       <  6:583036/140‑1 (MQ=255)
                                                                                                    cGTCTCGCAGTTTTTTTTAAATTGTGCCGA‑GTAATTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGATATAGTGAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                       <  6:520880/140‑1 (MQ=255)
                                                                                                    cGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAC‑CGCTAAAGTGAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                       <  2:737472/140‑1 (MQ=255)
                                                                                                     gTCTCGCAGTAGTTTTTAATATGTGCCGG‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAACTGAAAAAACAATAGCTTACTCATCAATTTCGTGTATCAGGACGAAATTCAGTGTTTGTAGTGATgatt                                      >  7:361294/1‑137 (MQ=255)
                                                                                                       ctcGCATTATTTTTTAATATGCGCCGC‑GTTTTACATATGATGCTGCCCCAAAAAAATAAAAA‑CGCTAAAGTTAAAAACCAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                    <  2:491486/140‑1 (MQ=255)
                                                                                                       ctcGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                    <  6:465799/140‑1 (MQ=255)
                                                                                                       ctcGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGG‑CCCAAAAAATAAAAACCCCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                    <  6:223495/140‑1 (MQ=255)
                                                                                                        tcGCAGTTGTTTTTAATTTGTGCGGC‑GTATTTCCTATGATGCTGGCACAAAAAAATAAAAC‑CGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTC‑‑GATGCTGATAat                                 <  2:948947/140‑1 (MQ=255)
                                                                                                         cGCATTAGTTTAAAATAGGGCCCGC‑ATATTTCATAAGATCCGGCCCCAAAAAAAAAAAAA‑CCATAAATTCAAAACACAAAACAAGACTCCGCAATATCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATaa                                  <  3:282851/140‑1 (MQ=255)
                                                                                                          cacgtaggTTTAAATATGGGCCGG‑GTATTTCCTATGATGATGGCCCCAAAAAATAAAAA‑CGCTAAAGTTAAAAAACAAAAGCAGACTCCTCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAat                                 <  4:331768/137‑1 (MQ=255)
                                                                                                            ttttgtTTTTAATATGTGCGGC‑TTATTACCTTTGATGACGGCCCAAAAAAATAAAAA‑CTATAATTTTAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                               <  8:721667/136‑1 (MQ=255)
                                                                                                            tttattttttAATATGTGCCGG‑TTATTTCATTTGATGCTCGCCCAAAAAAATAAAAA‑CGATATTGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                               <  1:820308/135‑1 (MQ=255)
                                                                                                            tgtagtTTTTAATTTGTGGCGC‑GTTTTTACTTTGATGCCGGCACAAAAAACAAAAAT‑CTCAATAGTTAAAAACAAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                               <  1:771609/139‑1 (MQ=255)
                                                                                                              ttttttttAAATTGTGCCTC‑TTATTTAATTTGATGCTGGCCGAAAATAATAAAAA‑GGCTAATGTAAAAAAACAAAAGAAGACTCCGGAATTTCGTGTATCATGACGAAATTCATTGTTTGTCTTGATGCTGATAattatt                             <  8:662785/137‑1 (MQ=255)
                                                                                                              tagtTTTTTATTTGTGCCTC‑GTATTACATAAGCTGCTGGCCCAAAAAAATAAAAA‑CGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAattatt                             <  6:788711/140‑1 (MQ=255)
                                                                                                               tgtTTTTAATATGTGCCGC‑TTATTTCCTATTATGCTGGCCCAAAAAAATAAAAA‑CGCTAATGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATtg                            <  1:100598/139‑1 (MQ=255)
                                                                                                                gtTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATtgt                           >  5:418480/1‑140 (MQ=255)
                                                                                                                   tttATTTTGTGCCGA‑GTATTTCCTTTTATGGTGGCCAAAAAAAATAAAAA‑CGCTAATTTGAAAAACCAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                        <  7:105494/140‑1 (MQ=255)
                                                                                                                   tttAATTTGTGCATA‑GTTTTTCATTTGATGTTTGCACAAAAAAATAAAAA‑ATCTATAGTATAAAAAAAAATGAAGACTCCGCAATTTCGTTAATCAGGACGAAATTCATTGTTTGTCGTTATGCTGATAATTATTGTggg                        <  7:105498/140‑1 (MQ=255)
                                                                                                                   tttAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                        >  3:739201/1‑140 (MQ=255)
                                                                                                                     tattttgtcCCGC‑GTTTTTCTTATGATGCTGGCCCAAAAAAATAAAAA‑CGCAAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  2:627200/135‑1 (MQ=255)
                                                                                                                     tAATATTTGCCGA‑GTATTTCATATGATGCTGTCCCCAAAAAATAAAAA‑CGCTAAAGTTAAAAAACAAAAGAAGTCTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  8:536533/140‑1 (MQ=255)
                                                                                                                     tAATATGTGCCGC‑GTATTTCCTTTGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  8:890919/140‑1 (MQ=255)
                                                                                                                     tAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCAAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  4:673723/140‑1 (MQ=255)
                                                                                                                     aaaTTTGTGCCGC‑GTATTTCCTTAGATGCGGCCCAAAAAAAATAAAAA‑CGCTAAAGTTAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  2:345265/139‑1 (MQ=255)
                                                                                                                     aaaTTTGTGCCGC‑GTATTTCCTATTAGGCTGCCCCAAAAAAAAAAAAA‑CCCAACAGATAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  3:902090/139‑1 (MQ=255)
                                                                                                                     aaaTATTTGCCGC‑GTATTGCCTAAGATGCGGGCCCCAAAAAATAAAAC‑CCCTAACGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  6:175469/139‑1 (MQ=255)
                                                                                                                     aaaTAAGTGCCGC‑TTATTTCATTTGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                      <  7:181447/139‑1 (MQ=255)
                                                                                                                        tatGTGCCGC‑GTATTTCCTATGATGCTGGGCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGt                   >  5:296758/1‑140 (MQ=255)
                                                                                                                        tatGTGCCGC‑GTATTTCATAAGATGCTAGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGAGGCAGATAATTAAGGTGGGATggg                   >  1:619710/1‑139 (MQ=255)
                                                                                                                           gtgCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                >  8:730650/1‑140 (MQ=255)
                                                                                                                           gtgCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑AGCTAAAGTGAAAAAACAAAAGAAGCCTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATAGTGGGATGGTGcc                >  7:540540/1‑140 (MQ=255)
                                                                                                                           gtgCCGC‑GTATTTCATATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGAACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                <  5:536226/140‑1 (MQ=255)
                                                                                                                           gtgCCGC‑GTATTACATTTTATGCTGCCCAAAAAAAAAAAAAC‑CCCTAAAGTTAAAAAAAAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                <  5:2170/140‑1 (MQ=255)
                                                                                                                           gcgcggc‑gTTTTTCCTATGCCGCGGGCCCCAAAAAATAAACT‑CGCTAACGTTAAAAAACAAAAGAAGACTCCGCACTTTCGTGAATCAGGCCGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                <  3:146592/135‑1 (MQ=255)
                                                                                                                            tgCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGCCTCCGCAATTTCGTGAATCAGGACGAAATTCAATGTTTGTCGTGATGCTGATAATTATTGTGGGATGTTGcgg               >  7:728376/1‑138 (MQ=255)
                                                                                                                            tgCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCt               >  5:99053/1‑140 (MQ=255)
                                                                                                                             gCCGC‑TTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAACAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTg              <  6:556397/140‑1 (MQ=255)
                                                                                                                             gCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTg              >  7:729761/1‑140 (MQ=255)
                                                                                                                             gCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTg              >  1:387831/1‑140 (MQ=255)
                                                                                                                                 c‑gTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGCTGCTGATAATTATTGTGGGATGGTGCCTGgtgt          >  8:727983/1‑140 (MQ=255)
                                                                                                                                   gTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGtt         >  2:183888/1‑140 (MQ=255)
                                                                                                                                      tttCCTTTGCTCCTGCCCCAAAAAAATAAAAA‑CGCAAACGTGAAAAACCAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc      <  5:339871/140‑1 (MQ=255)
                                                                                                                                      tttCCTATGCTGCTGGCCCCAAAAAATAAAAA‑CGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc      <  8:575640/140‑1 (MQ=255)
                                                                                                                                      tttCATATTATGCTGCCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc      <  6:241290/140‑1 (MQ=255)
                                                                                                                                      tttCATATTATGCCGCCCACAAAAAAAAAAAA‑CGCTAATGTCAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc      <  2:553073/140‑1 (MQ=255)
                                                                                                                                      tttCATATGATGCTGGCCCAAAAAAATAAAAA‑CGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc      <  3:920150/140‑1 (MQ=255)
                                                                                                                                      ttaccTATGATGCTGGCCAAAAAAAAAAAAAC‑CCAAAAAGATAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc      <  5:713964/137‑1 (MQ=255)
                                                                                                                                       taccTTTGATGCTGGCCCCAAAAAATAAACA‑CCCTAAAGTTAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGGGATGCTGATAATTATTGTGGGATGGTGCCTGGGGTTCGcc     <  7:392301/138‑1 (MQ=255)
                                                                                                                                         ccTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGGTCGCCAg   >  6:138993/1‑140 (MQ=255)
                                                                                                                                          cTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGt  <  6:925901/140‑1 (MQ=255)
                                                                                                                                          cTATGATGCTGGCCCAAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGt  <  6:744977/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TACACCTACGGTCTGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGC‑GTATTTCCTATGATGCTGGCCCCAAAAAATAAAAA‑CGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGT  >  NZ_CP022686/1504187‑1504463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A