Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,504,339 C→A 19.7% P259Q (CCA→CAA)  CIW80_RS07520 → YgcG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,504,3390CA19.7% 233.8 / 17.9 131P259Q (CCA→CAA) CIW80_RS07520YgcG family protein
Reads supporting (aligned to +/- strand):  ref base C (55/47);  new base A (2/27);  total (57/74)
Fisher's exact test for biased strand distribution p-value = 3.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCA  >  NZ_CP022686/1504200‑1504478
                                                                                                                                            |                                                                                                                                           
tGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCCATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATTTGCCGCGTATTTCCTATTATTCTGGcccc                                                                                                                                             >  2:166188/1‑140 (MQ=255)
 ggTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGGTTATTTTTCGCAGCAATCTGCTGGCAGCGTATCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTTTGATGCTGGCCCCa                                                                                                                                            >  8:349256/1‑140 (MQ=255)
        cTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTGTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCCGTAGGTTTTAA‑TATGTGGCGCGGATTTCCGAAGATTCTGGGCCCACCAAATa                                                                                                                                     >  2:1093704/1‑140 (MQ=255)
         tGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATaa                                                                                                                                    <  7:207722/140‑1 (MQ=255)
          gCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTAGCAGCAAACAGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTTCCCCGGATTTACTATGATGCTTGCCCCAAAAAATa                                                                                                                                     >  4:601101/1‑138 (MQ=255)
            tttCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTGTTTTTTTAGCAGCAATAAGCAGGCAGCGTCGCGCAGGAGTTTTTTA‑TATGATGCGCGGATTTGCGATGATGCTTGCCCCAAAAAATtaaaa                                                                                                                                 >  2:490833/1‑140 (MQ=255)
                  gACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCt                                                                                                                             <  3:508904/138‑1 (MQ=255)
                  gACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGGCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTaa                                                                                                                           <  6:998018/140‑1 (MQ=255)
                       tGTCATCATTCGGCGGGTGTCGGTCGTGTGGTTTAGGTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGGATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGt                                                                                                                        >  3:830708/1‑138 (MQ=255)
                        gTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTAGCAGCAATCTGCTGGCCGCGGCTCGCAGTAGTTTTTTA‑TATGTGCCGCGTATTTAGTATGATGCTGGGCCCAAAAAATAAAAACGCTTAAGTGaa                                                                                                                     >  2:242121/1‑140 (MQ=255)
                          catcatTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGaaaa                                                                                                                   <  2:544865/140‑1 (MQ=255)
                            tcatTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTTA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGaaaaaa                                                                                                                 <  2:152976/140‑1 (MQ=255)
                            cagttCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCATTCTGCGGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGaaaaaa                                                                                                                 <  7:1039062/137‑1 (MQ=255)
                                      gggggcggtcgTTTGTTTTATTTTTTTTTTCGCAGCAATCTGCTGGCTGCGTCGCGCTGTTGTTTTTAA‑TATGGGCCGCGTATGACCTATGTTGCGGCCCCCAAAAAATAAAAACGCAAAAGTGAAAAAACAAAcgaaga                                                                                                       <  1:744255/135‑1 (MQ=255)
                                        tgtCGGTCGTTTGTTTTATTTTATTTTTAGCAGAAATCTGCTGGCAGCGTCGCGCAGTAGTTTTTAA‑TATGTGCCGCGGATTTACTATTATGCTGGCCCCAAAAAATAAAAAAGCTAAAGTGAAAAAACAAAAGAAGAca                                                                                                     >  2:661318/1‑139 (MQ=255)
                                              tagtttttttTAGTTTATTTTTCGCTGAAATCTTCTTGAAGCTTCTCGCTTTATTTTTTTA‑TATGTGCGGCTTATTTCATATGATGCTGGCCAAAAAAATTAAAAACTATTAATTTAAAAAACAAAAGAAGACTCCGCaa                                                                                               <  7:1045316/138‑1 (MQ=255)
                                                 tttgtttTAGTTTATTTTTATCAGCAATCTGCTGGCAGCCTCTAGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTACTATTATGATGTACCCAAAAAATAAAAAATCGAAAGTTAAAAAACAAAAAAAAACTCCGAAAatt                                                                                            >  2:790005/1‑140 (MQ=255)
                                                   tgtttTAGTTTATTTTTCGACGCAATATGCGGGCAGCGTCTCGCATTATTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGGTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCg                                                                                          <  8:886249/140‑1 (MQ=255)
                                                          gTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAACCGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCa                                                                                   >  2:1097450/1‑140 (MQ=255)
                                                          gTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAAtaa                                                                                   >  1:999813/1‑138 (MQ=255)
                                                            ttATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAAAAAAAGAAGACTACGCAATTTCGTGAATCa                                                                                   >  4:256391/1‑138 (MQ=255)
                                                              ttttttCGCCGCAATCTGCTGGCCGCGTCTCGAAGTTGTTTTTAA‑TATGTGCCGCGTATTACCTATGATGCGGGCCCAAAAAAAAAAAACGGCTAACGTCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAgg                                                                                 <  3:344011/137‑1 (MQ=255)
                                                               ttttgCGCAGCAATGTGCTGGCAGGGTCTCCCAGTATTTTTTAA‑AGTGTCCCGCGTATTTCCTATGATGCTGGCCCCAAAAAAAAAAACCGCTAAAGCAAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACg                                                                              <  2:868109/140‑1 (MQ=255)
                                                                    cGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGGGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAG‑AGACTCCGCAATTTCGTGAATCAGGACGAAATTc                                                                        <  2:784824/140‑1 (MQ=255)
                                                                      cagcaATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TTTGTGCCGCTTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCa                                                                       <  2:98788/140‑1 (MQ=255)
                                                                      cagcaATCTGCTGGCAGCGTCTCGCAGTAGTGTTTAA‑TATGTGCCGCGTATTTCCTATGATGCAGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAAtt                                                                         >  3:1157964/1‑138 (MQ=255)
                                                                          aaTCTGCTGGCAGCGTCTCGCAGTATTTTTTAA‑TAGGGGCCGCGTATTTCCTTTTATGCTGCCCCAAAAAAATAAAAACTCAAAAGTCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCAtt                                                                     <  4:1132501/138‑1 (MQ=255)
                                                                           aTCTGCGGGCAGCGTCTCGCATTAGTTTTTAA‑TTTGGGCCGCGTATTTCCTATGATGCGGGCCCCAAAAAATAAAACCGCTAAAGTCAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCAttg                                                                    <  3:299600/138‑1 (MQ=255)
                                                                                  ggCTGCGTCTCGGATTAGTTTTTAA‑TATTGGCCGCGTTTTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAACCAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCg                                                             <  4:362351/138‑1 (MQ=255)
                                                                                   gCAGCGTCTCGAATTCTTTTAAAA‑TATGAGCCGCTTATTTCCTATGATGAGGTCCACAAAAAATAAAAACGCTAAATTCAAAAAAAAAACTAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGa                                                          <  5:245506/140‑1 (MQ=255)
                                                                                     tgCGTCTCGCATTTGTTTTAAA‑TATGGGCCGCGTTATTCCTATGATGCGGGCCCCAAAAAAAAAAAACGAAAAAGTCAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                        <  5:813992/139‑1 (MQ=255)
                                                                                     cgCGTCTCGCAGTCTTTTTTAA‑TATGTGCGGCGTATTTCCTCTTATGCTGGCCCAAAAAAATAAAAACGCTAAATTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGa                                                          <  3:680221/137‑1 (MQ=255)
                                                                                     aggggctcGCTGTATTTTTTAA‑TATGTGCCGTGTATTACTTATTATGCTGGCCCCAAAAAATAAAAGCGCTATAGTTAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCTGGACGAAATTCATTGTTTGTCGTGa                                                          <  3:640335/133‑1 (MQ=255)
                                                                                     aGCGTCTCGAAGTATTTTTTAA‑TATGTGCCGCGTATTTCATATTATGCTGCCCCAAAAAAAAAAAAACTCTAAATTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                        <  6:1061931/140‑1 (MQ=255)
                                                                                     aGCGTCTCGAAGTAGTTTTTAA‑TTTGTGCCGCTTTTTTCCTATTATGCTGCCCCCAAAAAAAAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                        <  2:416913/140‑1 (MQ=255)
                                                                                       ggTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGTTGCTGGCCCCAAAAAAAAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                        <  4:569176/137‑1 (MQ=255)
                                                                                       cGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTAATTCCTATGATGCTGGCCCCAAAAAATAATAACGATAAAGTGAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTAATTGTTGTTCGTGATgat                                                      >  5:611398/1‑138 (MQ=255)
                                                                                        gTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAACCGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGGTTGTCGGGATgg                                                       >  3:814181/1‑137 (MQ=255)
                                                                                          ctgccAGTTGTTTTAAA‑TATGGCGCGCGTATTTCTTATGTGGCGGGCCCAAAAAAAAAAACACGCTAAATTTAAAAAAAAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                   <  1:260058/136‑1 (MQ=255)
                                                                                          ctcGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                   >  2:311728/1‑140 (MQ=255)
                                                                                          ctcGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTAGTGAAGCAGGACGAAATTCATTGTTTGTGGTGATGCTGAt                                                   >  5:950240/1‑140 (MQ=255)
                                                                                          ctcGCAGTAGTTTTAAA‑TATGTCCCGCGTATTTCCTATGATGCTGCCCCCAAAAAATAAACACGCTAAAGTCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTg                                                     <  3:855891/138‑1 (MQ=255)
                                                                                            ggctgtattTTTTAA‑TTTGCGCCGCGTATTTCCTATGATGCTGCCCCCAAAAAATAAAAACTCTAAAGTCAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                   <  4:318999/134‑1 (MQ=255)
                                                                                            cGCAGTAGTTTTTAT‑TATGTGCCGCGTATTTCCTATGATGCGGCCCCCAAAAAATAAAAACGCTAAAGTGAAAAACAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATaa                                                 <  8:218835/140‑1 (MQ=255)
                                                                                            cGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGCTGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                   <  4:425777/138‑1 (MQ=255)
                                                                                            cGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCATATGATGCTGGCCCAAAAAAATAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATaa                                                 <  8:508002/140‑1 (MQ=255)
                                                                                               tttagtTTTAAA‑TATGTGCCGCTTATTTCCTATCTAGATGGCACCAAAAAATAAAACCGCAAAAGTGAAAAAAAAAATCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                                              <  7:302603/138‑1 (MQ=255)
                                                                                               tgtagtTTTTAA‑TATGTGCCGCGTTTTTCCTATGATGCTGGCCCCAAAAAATAAAAACCCTAAAGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAat                                                <  3:189779/137‑1 (MQ=255)
                                                                                               atttgtTTTTAA‑TTTGTGCCCCTTTTTTCTTATTATGCTGGCCCAAAAAAATAAAACCGCTAAATTTAAAAAACAAAAGAAGACTCCGCAATTTCGTCAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                                              <  1:601386/136‑1 (MQ=255)
                                                                                               agtagtTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                                              <  2:697485/140‑1 (MQ=255)
                                                                                               agtagtTTTAAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCAAAAAAAAAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAat                                                <  4:1107378/138‑1 (MQ=255)
                                                                                                tgtttttttAATTTTGTGCCGCGTATATCATATGATGCTGGCCCAAAAAAAAAAAAACGATAAAGAAAAAAAAAAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGCGATGCTGATAatta                                              <  5:805301/136‑1 (MQ=255)
                                                                                                 tagtTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAattatt                                            >  5:47050/1‑140 (MQ=255)
                                                                                                  tgtTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAACCGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATtg                                           <  6:644550/139‑1 (MQ=255)
                                                                                                  agtTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCAAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAattat                                             <  4:1139887/138‑1 (MQ=255)
                                                                                                      tttAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                       >  5:891195/1‑140 (MQ=255)
                                                                                                      tttAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                       <  6:30530/140‑1 (MQ=255)
                                                                                                        tca‑tagGTGCAGAGTATTGATTATTATGCTGGCCCAAAAAAACAAACACGATAACTTCAAATAAAAAAAGAAGAGTCCGCATTTACTTGAAGCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggt                                       <  4:294756/136‑2 (MQ=255)
                                                                                                        tAA‑TATGTGCCGCTTATATCCTATTATGCTCCCCAAAAAAAAAAAAACCGCTAATGTCAAAAAAAAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                     <  7:610807/140‑1 (MQ=255)
                                                                                                        aaa‑TTTGTGCGGCTTATTTCCTATGTTGGGGGCCACTAAAAATAAAAACGCTAATGTCTAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                     <  2:338790/139‑1 (MQ=255)
                                                                                                        aaa‑TTTGGGCCGCGTATTTCCTATGATGCAGCCCCAAAAAAAAAAAACCGCTAACGTCAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                       <  3:479114/137‑1 (MQ=255)
                                                                                                        aaa‑TATGTCCCGCTTATTTCATTTGATGCTCGCCCAAAAAAAAAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                     <  1:993864/139‑1 (MQ=255)
                                                                                                         aa‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATg                                    >  2:835351/1‑140 (MQ=255)
                                                                                                          aatagcggCCGCGTATTTCCTATGATGCTCGCCCCAAAAAAAAAAAACGCAAATGTTAAAAAAAAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATg                                    <  5:966259/133‑1 (MQ=255)
                                                                                                               gttccGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTAGTGAATCAGGAATAAATTAATTTTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                               >  2:91115/4‑140 (MQ=255)
                                                                                                               gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                               <  6:830853/140‑1 (MQ=255)
                                                                                                               gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAATACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTTTGGGATGGTGcc                               >  5:787658/1‑140 (MQ=255)
                                                                                                               gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                               >  1:629280/1‑140 (MQ=255)
                                                                                                               gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACCCTAAAGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                               <  8:674868/140‑1 (MQ=255)
                                                                                                                tgCCGCGTATTTCCTATGATGCAGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACACCGCAATTTCGTGAATCAGGACGAAATTCAATGTTTCTCGAGATGCTGATAATTATTGTGGGATGGTGCCt                              >  5:569325/1‑140 (MQ=255)
                                                                                                                 gCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                       >  3:1024377/1‑130 (MQ=255)
                                                                                                                 gCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                       <  4:1024377/130‑1 (MQ=255)
                                                                                                                     cgTTTTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGAAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGgtgt                         >  1:1141971/1‑140 (MQ=255)
                                                                                                                       tATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                       >  8:259783/1‑140 (MQ=255)
                                                                                                                         tttCCTTTGATGAGGGCCCCAAAAAAAAAAAACGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                       <  4:325727/138‑1 (MQ=255)
                                                                                                                         tttCCTATTCTGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                       <  4:640954/138‑1 (MQ=255)
                                                                                                                         tttCCTATTATGCTGGCCCAAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                     <  1:1050719/140‑1 (MQ=255)
                                                                                                                         tttCCTATTATGCTGCCCCCAAAAAATAAAAACGATAAATTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                     <  8:410122/140‑1 (MQ=255)
                                                                                                                         tttCCTATGATGCTGGCCCAAAAAAATAAAACCCATAAAGTCAAAAAAAAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                       <  3:280079/138‑1 (MQ=255)
                                                                                                                         tttCCTATGATGCTGGCCCAAAAAAATAAAAACGCTAAAGTGAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                       <  4:1145565/138‑1 (MQ=255)
                                                                                                                         tttCATATGCTGCGGGCCCCAAAAAATAAAACCGCTAACGTCAAAAAACACACGAAGACTCCGCAATTTCGTGACTCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                     <  5:1008087/140‑1 (MQ=255)
                                                                                                                         tttCATATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                       <  4:517679/138‑1 (MQ=255)
                                                                                                                           acacatgatgCTGCCCCCAAAAAATAAAAACGCTAAAGTGAAACAACAAAACAACACTCCTCAATTTCGTGAATCAGGACGAAATTCATTGTTTGGCGGGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCa                   <  7:68814/136‑1 (MQ=255)
                                                                                                                             gtttgatgCGGGCACAAAAAAAAAAAAACTATAAAGTCAAAAACCAAATGAAGTCTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATTATTATTGTGGGATGGTGCCTGGTGTTCGCCa                   <  3:1132765/135‑1 (MQ=255)
                                                                                                                               atgatgCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAATACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTTCCTGGTGTTCGCCCGtaa               >  7:254355/1‑140 (MQ=255)
                                                                                                                               atgatgCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaa               >  7:1008105/1‑140 (MQ=255)
                                                                                                                                  ttgCTGGACCAAAAAAATAAAACCGATAAAGTGAAAAAAAAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaataa            <  2:707823/139‑1 (MQ=255)
                                                                                                                                  atgCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaataa            >  2:418282/1‑140 (MQ=255)
                                                                                                                                  atgCTGCCCAAAAAAAAAAAAAACGCAAAAGTCAAAAAAAAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaataa            <  1:166188/140‑1 (MQ=255)
                                                                                                                                    gCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTAGCCAGTAATaaat          >  7:376845/1‑139 (MQ=255)
                                                                                                                                    gCTGGCCCAAAAAAATAAAAACGCTAAGGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGAAGATATTCATTGTTTGTCGTGATGCTGATAATTATTGTTGTATGTTGCATGGTGTTCTCAAGTTATaaat          >  7:280058/1‑139 (MQ=255)
                                                                                                                                      tGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGat        >  5:617304/1‑140 (MQ=255)
                                                                                                                                       gcccccAAAAAATAAAAACGACAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGGGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata       <  2:876184/138‑1 (MQ=255)
                                                                                                                                       gcccccAAAAAAAAAAAACGAAAATGTTAAAAAAAAAAAGAAGACTCCGCAATTTAGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata       <  5:541556/138‑1 (MQ=255)
                                                                                                                                       gccccaAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata       >  7:429503/3‑140 (MQ=255)
                                                                                                                                        gCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGatat      >  1:1115311/1‑140 (MQ=255)
                                                                                                                                        gCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGatat      >  6:214235/1‑140 (MQ=255)
                                                                                                                                          cccAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTACGCAATATCGTGAATCAGTACTAAATTCATTGTTTGTCTTGATGCTGATAATTATTGAGGCATCGTGCCTGGAGTTCTCCATGAATAACGatatat    >  1:1124267/1‑138 (MQ=255)
                                                                                                                                            cAAAAAATAAAACCGCAAACGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCa  <  2:715785/140‑1 (MQ=255)
                                                                                                                                            cAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCTTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCa  >  7:679185/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TGGTTTTTCTGCTTTCAGGACGATGTCATCATTCGGCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAA‑TATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCA  >  NZ_CP022686/1504200‑1504478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A