Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F2 I0 R1	252	7301.5	9636800	98.5%	9492248	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	1,504,373	G→C	9.9%	K270N (AAG→AAC)	CIW80_RS07520 →	YgcG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	1,504,373	0	G	C	9.9%	349.3 / 11.5	168	K270N (AAG→AAC)	CIW80_RS07520	YgcG family protein
Reads supporting (aligned to +/- strand): ref base G (58/91); new base C (3/15); total (61/107)
Fisher's exact test for biased strand distribution p-value = 7.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

GGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAATTGATC  >  NZ_CP022686/1504238‑1504509

ggtgggggtggtTTGTTTTAGTTTTTTTTTCGCAGCAATGTGCTGGCTGCGTCTCGAAGTTGTTTTTAATATGTGCGGCGTATTTCCTATGTTGCTGGCCCCAAAAAAAAAAAACGCTAAAGTGAAAAAACAAAagaaga                                                                                                                                      <  5:296781/134‑1 (MQ=255)

ggTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTTAAAAAACAAAacaaga                                                                                                                                      <  6:345549/140‑1 (MQ=255)

           tttgtttTAGTTTAATTTTAGCAGCAATCTGCAGGCAGCGTCTCGCAGTAGTTTTTAATAAGTGCCCAGATTTTCCTATCATGATGGCCCCAAAAAATAAAAACGCAAAAGTTAAAAAACAAAACAAAACTCCGCAAttt                                                                                                                           >  4:893346/1‑140 (MQ=255)

           tgtgtttTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTACTATTATGCTGGCCCCAAAAAAAAAAAACGCAAAAGTGAAAAAAAAAAAGAAAACTCCCCAAttt                                                                                                                           >  6:315533/3‑140 (MQ=255)

                  ttgTTTATTTTTCGCATCTATCTGCTGGCTGCGTGGCGCTGTAGTTTTTAATAGGTGCCGCTTATTTCATATTATGCTGGCCCAAAAAAAAAAAAACGCTAATGTGAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAAt                                                                                                                    <  4:206783/138‑1 (MQ=255)

                    gTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCa                                                                                                                  >  6:1098451/1‑140 (MQ=255)

                      ttATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAgg                                                                                                                >  4:197212/1‑140 (MQ=255)

                         tttttCGCAGAAATCTGCTGGCAGCGTCTCGCTGTAGTTTTTAATATTGGCCGCGTATTTCCTATGATGCTGGCCCAAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACg                                                                                                             <  8:116014/140‑1 (MQ=255)

                              cGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGCCTCCGCAATTTCGTGAATCAGGACGAAAtt                                                                                                        >  5:1166522/1‑140 (MQ=255)

                                cagcaATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGCCTCCGCAATTTCGTGAATCAGGACGAAAtt                                                                                                        >  2:512180/1‑138 (MQ=255)

                                            ggCTGCGTCTCGCTTTTTTTTTTAATATGTTCCCCTTATTTCCTATGATGCTGCCCCAAAAAAATAAAAACGCTAATGTAAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCg                                                                                            <  1:147052/138‑1 (MQ=255)

                                              caggctctcGCAGTAGTTTTAAATAGGTCCCGCTAATTTCCTATGAAGCTGCCCCAAAAAAATAAAAACTATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGAt                                                                                        <  6:16436/135‑1 (MQ=255)

                                               tgCGGCTCGCATTTTTTTTTAATTAGGGCCTCTTTTTGCCTATGATGCGGGCCCCAAAAAATAAAAACTCTAACGTGAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                       <  6:1182194/139‑1 (MQ=255)

                                               cgCGTCTCGCAGTTGTTTTTAATTTGTGCCGCTTATTTCCTAAGATGCTGCCCCCAAAAAAAAAACACGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                       <  3:1009472/139‑1 (MQ=255)

                                               cgCGTCTCGCAGTAGTTTTTAATATGTGCCTCTAATTTCATATGATGCTGCCCCAAAAAAATAAAAACCCTAAAGTTAAAAAAAAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                       <  3:335834/139‑1 (MQ=255)

                                               aGCGGCTCGCAGTAGTTTTTAATATGGGCCGCGTATTTCCTATGATGCTGCCCCCAAAAAATAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                       <  4:1029024/140‑1 (MQ=255)

                                                gCGTCGCGCAGTCGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGATAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGc                                                                                      <  3:1017530/140‑1 (MQ=255)

                                                 ctgctcGCCGTATTTTTTAATATGTGCCGCTTATTTCCAATGATGCTGGCCCAAAAAAATAAAAACGCTAACGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                                                                     <  4:984356/137‑1 (MQ=255)

                                                 cggcgcGCAGTTGTTATTATTATGTCCCGTGTCTTTCATATGATGATGCCCCAAAAAAAAAAAAACGATAAAGTTAAAACACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                                                                     <  7:598582/135‑1 (MQ=255)

                                                 cGTCTCGCTGTTGTTTTAAATATGTGCCGCGTATTTACTATGATGCTGGCCCAAAAAAATAAAAACGCTAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                                                                     <  3:508744/140‑1 (MQ=255)

                                                 cGTCTCGCAGTAGTTTTAAAAATGTGAAGCGAATTTCCAATGATGCTGGCCACAAAAAATAAAAACGCTAAAGTAAAAAAACAAAAGAATAATCCGCAATTTAGTGAATCACGACGAAATTCAGTGTTTGTCTTGATgag                                                                                     >  6:959232/1‑138 (MQ=255)

                                                 cGTCCCGCAGTAGTTTTTAATATGCGCCGCGTATTTCCTATCATGCTGGCCCAAAAAAAAAAAACCGCTAATGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                                                                     <  3:288513/140‑1 (MQ=255)

                                                  gTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAAGTTCGGGAATCAGGACGAAATTCATTGTTTGGCGTGATGGTg                                                                                    >  8:563081/1‑140 (MQ=255)

                                                  gTCTCGCAGTAGTTTTTAATATGTGCCGCGGATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTg                                                                                    >  8:478695/1‑140 (MQ=255)

                                                    ctcGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTg                                                                                    >  1:808224/1‑138 (MQ=255)

                                                    ctcGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTg                                                                                    >  1:564472/1‑138 (MQ=255)

                                                    ctcGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGAAGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                                                  >  4:626836/1‑140 (MQ=255)

                                                     tcGCAGTCGTTTTTAATATGTGCCGCGTATTTCCTATGATGCGGGCCCCAAAAAATAAAAACGCTAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATa                                                                                 <  7:395695/140‑1 (MQ=255)

                                                     cggCAGTTTTTTTTAATATGTGCCGCTTATGTCATATCAGGCTGGCCCAAAAAATTAAAAACGCAAAAGTGAAAAAAAAAATCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATa                                                                                 <  5:489688/138‑1 (MQ=255)

                                                      gcaagtagtTTTTAATATTATCCCGTTATTCCCAATTATCCTGCCCAAAAAAAATAAAACATCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATaa                                                                                <  6:382861/137‑1 (MQ=255)

                                                           tagtTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAattatt                                                                           >  6:461747/1‑140 (MQ=255)

                                                           tagtTTTTAATATGGGCCGCGTATTTCCTATTATGCGGCCCCAAAAAAATAAAAACGCTAAAGTCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAattatt                                                                           <  6:1224/140‑1 (MQ=255)

                                                            tgtTTTTAATATGTGCCGCGTATTTCCTATGATGCTGTCCCCAAAAAATAAAAACTCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATtg                                                                          <  4:1093533/139‑1 (MQ=255)

                                                              tttttAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCAATGTATGTCGTGATGCTGATAATTATtgtg                                                                        >  6:980079/1‑140 (MQ=255)

                                                                 ttattttgtgCCGCTTATTGACTTTGTTGCTGGCCCATAAAAATAATAAGGATAAATTGAAAAAACATAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGa                                                                     <  4:528045/134‑1 (MQ=255)

                                                                  tattatGCGCCGCGTATTTCCTAGCATGCGGGCCCCAAAAAATAAAAACGCAAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  8:358834/137‑1 (MQ=255)

                                                                  tAATTTGGGCCGGTTATTTAATATGATGCTGGCCCAAAAAAATAAAAACGATAAAGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  5:999795/140‑1 (MQ=255)

                                                                  tAATATTGGCCGTGTATTTCATATTTTGCTGCCCCAAAAAAATAAAAACCAAAATGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  3:109280/140‑1 (MQ=255)

                                                                  tAATATGTGCCGCTTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  8:745878/140‑1 (MQ=255)

                                                                  tAATATGTGCCGCTTATTTCCTATGATGCTGCCCCCAAAAAATAAAAACGCTAACGATAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  8:973994/140‑1 (MQ=255)

                                                                  tAATATGTGCCGCGTATTTCCTATTATGCTGGCCCCAAAAAATAAAAACGCTAATGTGAAAAAACAAAAGAAGACTACGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                                                      <  2:893964/138‑1 (MQ=255)

                                                                  tAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATTAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                                                      >  1:1001199/1‑138 (MQ=255)

                                                                  tAATATGTGCCGCGTATTTCCTATGATGCTGGCCCAAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  4:358771/140‑1 (MQ=255)

                                                                  tAATATGTGCCGCGTATTTCATATGATGCTGGCCCAAAAAAATAAAAACGATAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                                                      <  2:36249/138‑1 (MQ=255)

                                                                  tAATATGTGCCGCGTATTACATATGATGCTGCCCCCAAAAAAAAAAACCCCTAAAGTCAAAAAAAAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                                                      <  2:26957/138‑1 (MQ=255)

                                                                  tAATATGGGCCGCGTATTTCATATGATGCTGGCCCAAAAAAATAAAACCGCTAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  6:844803/140‑1 (MQ=255)

                                                                  aaaTATGTGCCGCGTATTTCATATGATGCAGGCCCCAAAAAAAAAAAACGCGAAAGTGAACAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  4:1125319/139‑1 (MQ=255)

                                                                  aaaTATGTCCCGCGTACTTCCTCTGATGCTGGCCCCAAAAAAAAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  4:61860/139‑1 (MQ=255)

                                                                  aaaTATGGGCCGCTTATTTCTTATGAGGGGGGCCCCAAAAAAAAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                    <  8:14753/139‑1 (MQ=255)

                                                                   aaTATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATg                                                                   >  7:323201/1‑140 (MQ=255)

                                                                   aaTATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATg                                                                   <  4:103983/140‑1 (MQ=255)

                                                                    atatGTGCCGCGTATTTCCTATTATGCTGGCCAAAAAAAAAAAAAACGCTAAAGTGAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATgg                                                                  <  8:1023601/140‑1 (MQ=255)

                                                                     tatGTGCCGCGTATTTCCTAACAAGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCTACACGAATTTCATAGTTTGGCGTGATGCTGAGAATTAATCTGGGATg                                                                   >  1:998956/1‑138 (MQ=255)

                                                                       tgtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGc                                                               >  5:317750/1‑140 (MQ=255)

                                                                        ttgCCGGGTATTTCATATGATGCTGGCCCAAAAAAATAAAACCGATAAATTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                                                              <  5:897760/139‑1 (MQ=255)

                                                                        gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                                                              >  5:325827/1‑140 (MQ=255)

                                                                         tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGTATGGTGCCt                                                             >  7:137155/1‑140 (MQ=255)

                                                                         tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGc                                                               >  2:1228016/1‑138 (MQ=255)

                                                                         tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGc                                                               >  1:70356/1‑138 (MQ=255)

                                                                             gcgTATTTCCTAAGATGCTGGCCCCAAAAAATCAAAACGCTAATGTGAAAAAACACACGAAGACTCCGCAAATACGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGgtg                                                         <  4:675869/140‑1 (MQ=255)

                                                                              cgTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATTCGTATAATTATTGTGGGATGGTGCCTGgggt                                                        >  3:360576/1‑140 (MQ=255)

                                                                                  tttcaattgatgCTGGCCCCAAAAAATAAAACCCCTAAAGTTAAAAACAAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                                                    <  8:715194/133‑1 (MQ=255)

                                                                                  tttCCTATTATGCTGCCACAAAAAAATAAAAAGGCAAACGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                                                      <  1:144646/138‑1 (MQ=255)

                                                                                  tttCCTATGATGCTTGCCCCAAAAAATAAAAACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                                                    <  4:677059/140‑1 (MQ=255)

                                                                                  tttCCTATGATGCGGGCCCCAAAAAAAAAAAACCCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                                                      <  2:184379/138‑1 (MQ=255)

                                                                                  ttaccTATTCTGCGGCCCCAAAAAAATAAAAACCCAAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                                                      <  1:12811/135‑1 (MQ=255)

                                                                                  ttaccTAAGATGCTGCCCCAAAAAAATAAAAACCCAAAAGTCAAAAAACAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                                                      <  1:370197/135‑1 (MQ=255)

                                                                                    tCCTATGATGCTGCCCACAAAAAATAAAACCGCTAAAGTGAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCa                                                  <  4:499353/140‑1 (MQ=255)

                                                                                      ataggatgCGGCCCCCAAAAAATAAAACCGCAAAAGTGAAAAAAAAAACGAAGACTCCGCAATTTAGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCa                                                  <  2:282710/134‑1 (MQ=255)

                                                                                          tttgCTGGCCCCAAAAAATAAAAACGATAAAGTGAAAAAACAACAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATTGTGCCTGGTGTTCGCCAGtaata                                            <  8:405012/138‑1 (MQ=255)

                                                                                           ttgCTGGCCCAAAAAAAAAAAAACGCAAAAGTGAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaat                                             <  2:572704/137‑1 (MQ=255)

                                                                                           atgCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaataa                                           >  5:155603/1‑140 (MQ=255)

                                                                                             gCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGACAGtaataa                                           >  1:958131/1‑138 (MQ=255)

                                                                                              cTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGa                                        >  4:706339/1‑140 (MQ=255)

                                                                                                ggCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                      >  4:342475/1‑140 (MQ=255)

                                                                                                ggCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                      <  8:743853/140‑1 (MQ=255)

                                                                                                ggCCCCAAAAAATAAAAACGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATATCGTGAATCAGGACGAAAATCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCGTTGTGTACGCCTGTAATAAAGata                                      >  6:857988/1‑140 (MQ=255)

                                                                                                ggCCCAAAAAAATAAAACCGCTAAAGTTAAAAAACAAAAGAAGACTCCTCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGa                                        <  2:230574/138‑1 (MQ=255)

                                                                                                gcccccAAAAAAAAAACATTCTACAGTTAAAAAAAAAAATAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                      <  8:1132056/138‑1 (MQ=255)

                                                                                                 gCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGCAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGatat                                     >  8:240775/1‑140 (MQ=255)

                                                                                                 gCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGat                                       >  1:221979/1‑138 (MQ=255)

                                                                                                     cAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCa                                 >  6:835063/1‑140 (MQ=255)

                                                                                                     aaaaaaaTAAAACCGGTAATCGTAAATAACATAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTAATTGTTTGGCGTGATGATGATAATTATTGTTGGTTGGTGCCTGGTGTTCGGCAGTAATTAAGATATTGCa                                 <  7:73049/139‑1 (MQ=255)

                                                                                                      aaaaaaTAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCAt                                >  4:559882/1‑140 (MQ=255)

                                                                                                       aaaaataaaaaataAAAAGATAAAAAAAAAAACAAGACTACGCAATTTCGTCAATCAGGACTAAATTCATTGTTTTTCGTTATGCTGATAAATATTGTTGGATGGTGACTTGTGTTCGCCAGTAATAAAGATATTGCAtt                               <  6:650197/140‑1 (MQ=255)

                                                                                                             aaaaaCGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                         <  4:277885/140‑1 (MQ=255)

                                                                                                             aaaaaCGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                         >  3:608409/1‑140 (MQ=255)

                                                                                                             aaaaaCGATAATGTTAAAAAACAAAAGACGACTCCGCAATTTCGTGAATCAGGACGAATTTCATTGTTTGTCGTGATGCTGATATTTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                         <  8:876128/140‑1 (MQ=255)

                                                                                                             aaaaaCGATAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                         <  4:774255/140‑1 (MQ=255)

                                                                                                               aacccatAAAGTCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTcaa                       <  5:1002809/134‑1 (MQ=255)

                                                                                                                aaCGCTAATGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTTCATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGGGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTcaac                      <  3:284550/140‑1 (MQ=255)

                                                                                                                     aaacgttaaaaaaCAAAAGAATACTCCGCAATTTCGTGAATCCGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatta                 <  8:717672/133‑1 (MQ=255)

                                                                                                                      aaaGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatt                  <  2:611774/138‑1 (MQ=255)

                                                                                                                      aaaGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatt                  <  1:897157/138‑1 (MQ=255)

                                                                                                                      aaaGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatt                  >  1:1046966/1‑138 (MQ=255)

                                                                                                                       aaGTGAAACAAAAAACGAAGACTCCGCAATTTCGTGAATCAGGACTAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatta                 <  2:1160707/138‑1 (MQ=255)

                                                                                                                           taaaaaaCAACAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGt           <  6:751426/139‑1 (MQ=255)

                                                                                                                           caaaacaAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTTCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTgg             <  1:893146/137‑1 (MQ=255)

                                                                                                                                    aaaGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAATTGAtg  >  4:1102948/1‑139 (MQ=255)

                                                                                                                                    aaaGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAATTGAta  >  4:460369/1‑139 (MQ=255)

GGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAAACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAATTGATC  >  NZ_CP022686/1504238‑1504509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A