Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,504,373 G→C 10.3% K270N (AAG→AAC)  CIW80_RS07520 → YgcG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,504,3730GC10.3% 379.5 / 6.8 190K270N (AAG→AAC) CIW80_RS07520YgcG family protein
Reads supporting (aligned to +/- strand):  ref base G (72/95);  new base C (2/17);  total (77/113)
Fisher's exact test for biased strand distribution p-value = 5.77e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAATTG  >  NZ_CP022686/1504235‑1504506
                                                                                                                                           |                                                                                                                                     
gcggGTGTCGGTCGTGTGTTTTAGTTTATTTTTCGCAGCAATCAGCTGGCATCGGCTCGCAGTATTTTTTAATATGTGCCGCGGATTTACAATTATGCTTGCCCCAAAAAATAAAA‑CCGCAAAAGTGAAAAAACAAaaga                                                                                                                                      >  8:499324/1‑140 (MQ=255)
     tgtCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTATAGCAGTAGTTTTTAATATGTGCCGGGTATTTACTATGAAGCTTGCCCCAAAAAATAAAA‑ACGCAAAATTGAAAAAACAAAATAacaca                                                                                                                                 >  2:874798/1‑139 (MQ=255)
         ggtcgTTTGTTTTAGTTTATTTTTCGCAGCAATATGCAGGCAGCGTCTCGCATTAGTTTTTAATATGTGCCGCGTATTTCCAATGAAGCTGGCCACAAAAAATAAAA‑AAGCAAAAGTGAAAAAACAAAAAAAGACTACGc                                                                                                                             >  8:1155387/1‑140 (MQ=255)
           tcgTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCTTTAGTTTTTAATTTGTGCGGGGTATTTCGTATGATGCTGGCCCAAAAAAATAAAA‑ACGCTAATGTTAAAAAACAAAAGAAGACTCCGCaa                                                                                                                           <  7:1008280/140‑1 (MQ=255)
              tttgtttTAGTTTATTTTTAGCAGCAAAAAGCTGGCACCCTCGCGCAGTAGTTTTTAATATTTTCCGCGGATTTCCTATGATTCTGGCCCCAAAAAAAAAAA‑ACACTAAATTTAAAAAACAAAAGAAGACTCCACAAatt                                                                                                                        >  8:126497/1‑140 (MQ=255)
                     tAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCTGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAAt                                                                                                                 <  2:622655/140‑1 (MQ=255)
                       gTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCAAAGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTACGTTAATCa                                                                                                               >  6:174688/1‑140 (MQ=255)
                           aTTTTACGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATTATGCTGCCCCCAAAAAATAAAA‑ACGCTAACGGCAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAgg                                                                                                             <  4:170040/138‑1 (MQ=255)
                                 cGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAAtt                                                                                                     >  1:251139/1‑140 (MQ=255)
                                 cGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAAtt                                                                                                     >  2:502771/1‑140 (MQ=255)
                                      caATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCAGCAATTTCGTGAATCAGGACGAAATTCAttg                                                                                                >  7:79731/1‑140 (MQ=255)
                                              tgGCAGCGTCTCGCAGTAGTTTTTAATATGTGACGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑AAGCTAAAGTTAAAAAACAAAAGAAGACTCCGCACTTTCGTGACTCAGGACGAAAATCAATGTTTGTCGt                                                                                        >  6:602520/1‑140 (MQ=255)
                                               ggCAGCGTCTCGCAGTTGTTTTTAATATGTGCCGGGTATTTCCTATGATGCTGGCCCAAAAAAATAAAA‑ACGCTAAATTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTg                                                                                       <  8:212320/140‑1 (MQ=255)
                                                gCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTgg                                                                                      >  1:399895/1‑139 (MQ=255)
                                                gCAGCGTCTCGCAGTAGTTTTTAATATGTGCAGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGt                                                                                        >  4:246115/1‑138 (MQ=255)
                                                  cgCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGa                                                                                      <  4:1196840/137‑1 (MQ=255)
                                                  aGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                    <  8:762244/140‑1 (MQ=255)
                                                  aGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCAAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTTTTTGTCGTGa                                                                                      >  3:383707/1‑138 (MQ=255)
                                                  aGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCGGGCCCAAAAAAATAAAA‑ACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                    <  7:374813/140‑1 (MQ=255)
                                                  aGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGATGGCCCCAAAAAATAAAA‑ACGCTAAATTGAAAAAACAAAAGAAGACTCCGCAATATCGTGAATCAGGACGAAATTAATTAGTTGTCGTgt                                                                                      >  3:1223198/1‑137 (MQ=255)
                                                  aGCGTCTCGCAGTAGTTTTAAATATGTCCCGCGTATTTACTATGATGCTGGCCCAAAAAAAAAAAA‑ACGCTAAAGACAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                    <  2:10719/140‑1 (MQ=255)
                                                  aGCGTCTCCCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCGGGCCCCAAAAAATAAAA‑ACGCAAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                    <  7:1070455/140‑1 (MQ=255)
                                                    ggTCTCGCTGTTTTTTTTAATATGGGCGGCTTTTTACCTATGTTGCTGGCCAAAAAAAATAAAA‑ACGCTAAAGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCt                                                                                  <  7:251720/139‑1 (MQ=255)
                                                    cggctcGCAGTATTTTTAAATTGGTGCCGCGTATATCCTATGCTGCTGGCCCAAAAAAAAAAAA‑ACGATAACGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                    <  3:1102477/135‑1 (MQ=255)
                                                    cGTATCGCAGTAGTTTTTATTATGGGCCGCGTATTTCCTATGTTGGTTGCCCAAAAAAATAAAA‑ACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATg                                                                                    <  3:856963/138‑1 (MQ=255)
                                                      tctcGCTGTTGTTTTAAATATGTGCCGGGTTTTTCCTATTATGCGGGCCCAAAAAAATAAAC‑ACCCTAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGa                                                                                <  1:510540/140‑1 (MQ=255)
                                                      tctcGAAGTATTTTTTAATATGTGCCTCGTATTTCCTCTCATTCTGCACCAAAAAAATAAAC‑ACGCAAAAGTGAAAAAACAAAAGAAGCCTCCGCAATTTCGAGAATCAGTACGAAATTCATTGTTTGTCGTGATGCTGa                                                                                <  6:1008509/140‑1 (MQ=255)
                                                      tcgcGCAGTTTTTTTTATTATGTGCAGCGTATTTCATTAGATGGGGCCCCCAAAAAATAAAA‑ACGAAAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGa                                                                                <  1:1262782/137‑1 (MQ=255)
                                                       ttctgtgtattTGTTAATATGTGCAGCGTATTTCATATTATGGTGGCCCCAAAAAATAAAA‑ACGATAAATTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGAAGATTTTCTTTTTTTTTCGTGATGCTGAt                                                                               <  2:852666/134‑1 (MQ=255)
                                                       ctcGCATTAGTTTTTAATATGTGCCGCTTATTTCCTTTGATGCTGGCCCAAAAAAAAAAAA‑ACGCTAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTg                                                                                 <  3:896230/138‑1 (MQ=255)
                                                       ctcGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGCCTCAGCAATTTCGTGAATCAGGACGAAATTAATTGTTTGTCTTGATGCTGAt                                                                               >  2:750493/1‑140 (MQ=255)
                                                         cGCAGTATTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGCCCCCAAAAAATAAAA‑CCGCTAACGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                                               <  4:227750/138‑1 (MQ=255)
                                                         cGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGCCCCCAAAAAATAATA‑ACGCTAATGGGAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATaa                                                                             <  8:397453/140‑1 (MQ=255)
                                                         cGCAGTAGTTTTTAATAGGTGCCGCGTATTTCCTATCATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGGTTGTCGTGATGCTGATaa                                                                             <  6:305813/140‑1 (MQ=255)
                                                          gCAGTAGTTTTTAATATGTGCCGGTTATTTCATATGATGCTGGCCCAAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAat                                                                            <  6:204507/140‑1 (MQ=255)
                                                            agtagtTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAGAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                                                                          <  6:999883/140‑1 (MQ=255)
                                                              ttgtTTTTAATTTGTGCCGCTTATTTCATATGATGCTGGCCCAAAAAAAAAAAA‑ACGATAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                                                                          <  4:924882/136‑1 (MQ=255)
                                                              tagtTTTTAATATGTGCCTCTTATATCATATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTCAAAAAACAACCGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAatta                                                                          <  4:36003/138‑1 (MQ=255)
                                                               tgtTTTTAATGTGTGCCGCTTTTTTCCTATGATGCTGGCCCAAAAAAATAAAC‑ACGAAAAATTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATtg                                                                       <  7:746319/139‑1 (MQ=255)
                                                                  ttatAATATGTGCCGCTTATTTCCTATGATGCTCGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAATACAAAAGAAGACTCAGCAATTTCTTGAATCAGGACGAAATTCATTGTTTGTAGTGATGATGATAATTATtgt                                                                      >  3:1017806/4‑138 (MQ=255)
                                                                   tttAATATGTCCCGCTTATTTCCTTTGTTGCTGGCCCCAAAAATAAAAA‑CCGATAAATTTAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGATATTCATTGTTTGTCGTGATGCTGATAATTATTGTggg                                                                   <  2:574261/140‑1 (MQ=255)
                                                                    ttAATTTGGCCCGCGTATTTCCTATGATGCTGCCCCCAAAAAATAAAA‑ACGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGa                                                                  <  8:118537/140‑1 (MQ=255)
                                                                    ttAATATGGGCCTCGTATTTCCTATTATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTTAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGa                                                                  <  8:1209671/140‑1 (MQ=255)
                                                                    ttAAAATGCCCCGGGTATTTCCTATGATGCTGGCCCCAAAAAATAACA‑CCGATAAAGTGAAAAAACAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGa                                                                  <  8:158529/140‑1 (MQ=255)
                                                                     tAATATTTGCCGCTTATTTCATTTGATGCTGGCCCCAAAAAATAAAA‑ACGATAAAGTGAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGAt                                                                 <  2:122214/140‑1 (MQ=255)
                                                                     aaaTTTGTGCCGCGTATTTCATATGATGCTCGCACCAAAAAAAAAAA‑ACTCAAAAGTTAAAAAACAAAACAACACTCCGCAATTTCGTGAATCAGCACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGaa                                                                 <  7:1017055/139‑2 (MQ=255)
                                                                           gtgCGGCTTATTTCCTATTATGCTGGCCCAAAAAAATAAAA‑ACGATAAAGTTAAAAACCAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCAGATAATTATTGTGGGATGGTGcc                                                           <  6:63298/140‑1 (MQ=255)
                                                                           gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTTAATCAGGACGAAATTCATTGTTTGTCGTGCTGCTGATAATTATTGTTGGTTGGTGcc                                                           >  1:622561/1‑140 (MQ=255)
                                                                           gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGCCGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                                                           >  1:976614/1‑140 (MQ=255)
                                                                           gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGcc                                                           >  8:1029089/1‑140 (MQ=255)
                                                                           gtgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTAGTGAATCAGGACGAAATTCATTGATTGTCGTGATGCTGATAATTATAGTGGGATGGTGcc                                                           >  5:1294509/1‑140 (MQ=255)
                                                                            tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATTAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACACCGCAATTTCGTGAATCAGGACGAAATTAATTGTTTGTCGTGATGCTGAGAATTAGTGTGGGTTGGTGACt                                                          >  7:1132344/1‑140 (MQ=255)
                                                                            tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                                               >  7:915424/1‑119 (MQ=255)
                                                                            tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGc                                                            >  4:1224676/1‑138 (MQ=255)
                                                                            tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGc                                                            >  4:668744/1‑138 (MQ=255)
                                                                            tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAAGTATTGTGGGATGGTGCCt                                                          >  5:511791/1‑140 (MQ=255)
                                                                            tgCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATAAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGCATGGTGCCt                                                          >  6:1260422/1‑140 (MQ=255)
                                                                               cgcgTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTg                                                         >  4:425335/1‑138 (MQ=255)
                                                                                   tttttCATATGAGGCTGGCCCAAAAAAAAAAAA‑ACCCAAAAGTTAAAAAACAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTc                                                   <  1:1005663/138‑1 (MQ=255)
                                                                                     tttCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAACAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                                                 <  6:1285721/140‑1 (MQ=255)
                                                                                     tttCCTATGAGGCTGCCCCCAAAAAAAAAAA‑ACCAAAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                                                 <  6:1189704/140‑1 (MQ=255)
                                                                                     tttCCCTTGATGCGGCCCCAAAAAAATAAAC‑ACGATAAAGTTAAAAAACAACAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGGCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                                                 <  7:995831/140‑1 (MQ=255)
                                                                                     tttCATATGATGCGGGCCACAAAAAATAAAA‑ACGATAAAGTTAAAAAACAAACCAAGACTCCGCAATTTCGTCAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGc                                                 <  1:186576/140‑1 (MQ=255)
                                                                                      ttCATATGATGCTGCCCCCAAAAAATAAAA‑ACGCAAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGcc                                                <  8:882608/140‑1 (MQ=255)
                                                                                       tCCTATGATGCGTGCCACAAAAAATAATA‑AATATAATTGTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATATTTATTGTGGGATGGTGCCTGGTGTTCga                                                 <  3:621225/138‑2 (MQ=255)
                                                                                         cTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAACCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCa                                               <  4:974346/138‑1 (MQ=255)
                                                                                              atgCTGGCCCAAAAAAATAAAA‑ACTCAAAAGTCAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGtaataa                                        <  8:25869/140‑1 (MQ=255)
                                                                                                gCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGAt                                                                               <  1:968704/99‑1 (MQ=255)
                                                                                                gCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAg                                      >  8:741936/1‑140 (MQ=255)
                                                                                                 gcggCCCCAAAAAAAAAAACCCGAAAATGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAg                                      <  5:717613/138‑1 (MQ=255)
                                                                                                   ggCCCCAAAAAATAAAA‑ACGCTAAAGTTAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                   <  2:958017/140‑1 (MQ=255)
                                                                                                   gcccccAAAAAATAAAA‑ACGCTAACGTTAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGGCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                   <  8:546839/138‑1 (MQ=255)
                                                                                                   gccccaAAAAAATAAAA‑CCGCTAAAGTGAAAAAACAAACGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                   <  5:1030820/138‑1 (MQ=255)
                                                                                                   gccacaaaaaaaTAAAA‑CCTATATATTTAAAAAACAAATGAAGACTCCGCAATTTCGCTAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGata                                   <  1:296722/134‑1 (MQ=255)
                                                                                                    gCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTTTGTGATGGTGCCTGGTGTTCGCAAGTAATAAAGatat                                  >  2:133556/1‑140 (MQ=255)
                                                                                                    gCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGAAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGTGATGGTGCCTGGTGTTCGCCAGTAATAATGatat                                  >  2:306884/1‑140 (MQ=255)
                                                                                                     ccccAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATAtt                                 <  1:502771/140‑1 (MQ=255)
                                                                                                      cccAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGTTAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGatat                                  >  4:1017739/1‑138 (MQ=255)
                                                                                                      cccAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTg                                                             >  3:739635/1‑111 (MQ=255)
                                                                                                        cAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCa                              <  8:416830/140‑1 (MQ=255)
                                                                                                        aaaaaaaaaaaa‑aCTATAAATTGAAAAAAAAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCa                              <  5:292471/139‑1 (MQ=255)
                                                                                                        aaaaaaaTAAAA‑ACGCTAAATTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCa                              <  7:520927/139‑1 (MQ=255)
                                                                                                          aaaaaTAAAC‑CCGCAAATTTCAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCAtt                            <  5:927776/140‑1 (MQ=255)
                                                                                                              aTAAAA‑ACGCTAATGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGGGATGCTGATAATTATTGTTGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtc                        <  8:712909/140‑1 (MQ=255)
                                                                                                                aaaa‑cCGCTAATGTGAAAAAAAAAACCAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                      <  1:525098/140‑1 (MQ=255)
                                                                                                                aaaa‑cCCCTAACGTCAAAAAACAAAACAAGACTCCGCAATTTCGTGAATCAGGCCGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                      <  1:735130/140‑1 (MQ=255)
                                                                                                                aaaa‑aCGCAAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCAtctc                      <  8:628340/140‑1 (MQ=255)
                                                                                                                  aa‑aCGCTAAAGTTAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTcaa                    <  8:359567/140‑1 (MQ=255)
                                                                                                                   cacgccTAAAGCGAACAACCAAACGAAGACGCCGACATTTCGTGAATCAGGCCGAAATTCATTGTTTGGCGTGATGCTGATAATTATTGTGGGATGGGGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTcaa                    <  2:1087418/135‑1 (MQ=255)
                                                                                                                    accgttAAAGTTAAAAAACAATCGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTcaac                   <  7:220256/135‑1 (MQ=255)
                                                                                                                        cTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatt               >  7:854969/1‑140 (MQ=255)
                                                                                                                          aaaGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAatt               >  3:943901/1‑138 (MQ=255)
                                                                                                                               taaaaaaTAAAAGAAGACTCCGCAATTTCGTTAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTgg          <  4:124059/137‑1 (MQ=255)
                                                                                                                               aaaaaaaCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGt        <  1:1022457/139‑1 (MQ=255)
                                                                                                                                aaaaaaCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGCTGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGtt       >  6:666798/1‑140 (MQ=255)
                                                                                                                                aaaaaaCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGtt       >  7:1264177/1‑140 (MQ=255)
                                                                                                                                aaaaaaCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGtt       >  2:130521/1‑140 (MQ=255)
                                                                                                                                aaaaaaCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAAGTCATTCTATGTCCTGATGATGATAATTATTGTGGGAGGGGGCCAGGGGTTCGCGAGTAATAAAGATAATGCATTCTACTCAACAATTAGTGTGtt       >  8:92652/1‑140 (MQ=255)
                                                                                                                                  aaaaCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGtt       >  4:744544/1‑138 (MQ=255)
                                                                                                                                     aCAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAAt    <  4:1348936/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
GCGGGTGTCGGTCGTTTGTTTTAGTTTATTTTTCGCAGCAATCTGCTGGCAGCGTCTCGCAGTAGTTTTTAATATGTGCCGCGTATTTCCTATGATGCTGGCCCCAAAAAATAAAA‑ACGCTAAAGTGAAAAAACAAAAGAAGACTCCGCAATTTCGTGAATCAGGACGAAATTCATTGTTTGTCGTGATGCTGATAATTATTGTGGGATGGTGCCTGGTGTTCGCCAGTAATAAAGATATTGCATTCATCTCAACAATTATTGGGTTAATTG  >  NZ_CP022686/1504235‑1504506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A