Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F13 I0 R1	150	9496.0	7621532	98.4%	7499587	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,951,545	A→C	15.4%	S47R (AGT→CGT)	topA →	type I DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,951,545	0	A	C	15.4%	192.1 / 3.7	131	S47R (AGT→CGT)	topA	type I DNA topoisomerase
Reads supporting (aligned to +/- strand): ref base A (65/37); new base C (2/20); total (67/64)
Fisher's exact test for biased strand distribution p-value = 2.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGT  >  NZ_CP022686/4951408‑4951684

tGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGCCCAGTGGCTCATCTGCC‑AAAAAGAGt                                                                                                                                           >  1:621150/1‑140 (MQ=255)

  ggTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGc                                                                                                                                         >  8:283839/1‑140 (MQ=255)

  ggTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGc                                                                                                                                         >  5:858883/1‑140 (MQ=255)

   gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGcc                                                                                                                                        >  3:101999/1‑140 (MQ=255)

   gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGcc                                                                                                                                        >  5:396435/1‑140 (MQ=255)

   gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGAGcc                                                                                                                                        >  3:307428/1‑140 (MQ=255)

    tAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCg                                                                                                                                       >  8:790334/1‑140 (MQ=255)

      aaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCgcc                                                                                                                                     >  2:742299/1‑138 (MQ=255)

      aaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGAc                                                                                                                                     >  1:645896/1‑140 (MQ=255)

      aaGCTCTTGTCATCGTTGAGTCCACGGCAATCGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGCAATCCAGCGTCGGTCACCTCAGCGATTTGACGTCCAGTGGCTCAACTGCG‑AAAAAGAGTGCCGAc                                                                                                                                     >  8:420959/1‑140 (MQ=255)

         ctctTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGActct                                                                                                                                  >  6:799699/1‑140 (MQ=255)

            ttGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTAcc                                                                                                                               >  3:962985/1‑140 (MQ=255)

            ttGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGACTGCCGACTCTAcc                                                                                                                               >  1:861382/1‑140 (MQ=255)

            ttGGCATCGTTGAGCCCCCGTAAAAACCAAAAACCATCAACAAGTATCTGGGTAGTGACTACGTGGTTAACCCCCGCGGCGGACACATCCGCGATTTGCCCCCCAGGGGCTCAACTGCC‑AAAAAGAGTCCCGACTCTAcc                                                                                                                               <  2:30312/140‑1 (MQ=255)

              gTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGCCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCG‑AAAAAGAGTGCCGACACTACCTc                                                                                                                             >  1:914173/1‑140 (MQ=255)

                   cGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGTTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGCATCTACATccaccc                                                                                                                        >  3:764879/1‑139 (MQ=255)

                   cGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAACTCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGAC‑AAAAAGAGTGCCGACTCTACCTccacca                                                                                                                        >  3:815873/1‑140 (MQ=255)

                    gctgcgTCCCCGGCACACCCCACAACTCTCAACAAGTATTGGGGTCTTGACTCGGGGGTTAAATCCCGCGGCGGTCACATCCGCGATTTGCCGACCTGTGGCTAAACAGCA‑AAAAAGCGTGCCGACTCTACCTCCACCaa                                                                                                                       <  6:131759/135‑1 (MQ=255)

                      tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCTACTCTACCTCCCCCAAGa                                                                                                                     >  6:923051/1‑140 (MQ=255)

                      tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGa                                                                                                                     >  1:136715/1‑140 (MQ=255)

                        aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTTCCGCCTCTACGTCCACCAAGACg                                                                                                                   >  6:529054/1‑140 (MQ=255)

                        aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTAAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACg                                                                                                                   >  8:29797/1‑140 (MQ=255)

                             ccGCCAAAAGCCAAAACGATCAACCTGTATCTGGGTTGTGACTACGTGGTGAAATCCAGCGTGGGTCACATCCGCGTTTTGCCGCCCAGTGGCTCAAATGCA‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTaa                                                                                                              <  8:659319/140‑1 (MQ=255)

                                    aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTTCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCAGACTCTACCTACACCAAGACGGCTAAAAAGcca                                                                                                       >  2:421745/1‑139 (MQ=255)

                                    aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCt                                                                                                       >  2:150095/1‑140 (MQ=255)

                                          aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCAGACCAGTGGCTCAACTTCC‑AAAAAGAGTGCAGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAg                                                                                                 >  1:822422/1‑140 (MQ=255)

                                             ctaTCAACAAGTATCTGGGTTGTTACTAGGTGGTGAAATCCCGCGGCGTTCACATCCGCGATTTGCCCACCTGTGGCCCAACTGCC‑AAAAAGCGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGAt                                                                                              <  1:751175/138‑1 (MQ=255)

                                               aTCAACAATTTTGTGTTTTGTGACTAGGTGTTTAAAACCAGCGGCGGTCACATCGGCTATTTGCGGACCTGTGGCTAAACTCCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGa                                                                                            <  3:947924/140‑1 (MQ=255)

                                                tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCCCATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGaa                                                                                           >  8:230938/1‑140 (MQ=255)

                                                tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCAGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGaa                                                                                           >  6:303063/1‑140 (MQ=255)

                                                  aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCAAGCGTCGGTCACATCCGCGATTAGACCACAACTGGCACAACTGAC‑AAAAATCGTGCAGACACTACCTCCACCAACACGGCTAAAAAGACTAAAAAGGATGAACg                                                                                         >  7:147864/1‑140 (MQ=255)

                                                    catgtttCTGGGTAGTGACTACGGGGATAAAACCAGCGGCGGACACCCCGGCTATTCGCCAACCAGTGGCTCAATTGCC‑AAAATGAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTg                                                                                       <  1:520009/134‑1 (MQ=255)

                                                       ttATGTGGGTAGTTACTCCGTGGTCAAAACCAGCGGCGTTCAAATCCGCGATTTGCCTCCCCGGGGCTAACATGCC‑AAAACCCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                                    <  4:541054/139‑1 (MQ=255)

                                                       gTATGTGGGTAGTGACTAGGTGGTTAAACCCAGCGGCGGTCACTTCCGCTTTTGGCCGACCAGTGGCTCAACTGAC‑AAAAAGCGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                                    <  2:387689/140‑1 (MQ=255)

                                                        tATCTGGGTAGTGACTACGTGTTGAAATCCCGCGGCGGTCATATCGCCGATTTGCCAACCTGGCGCCCTCCTCCC‑AAAAAGCGCGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcgc                                                                                   <  7:739036/140‑1 (MQ=255)

                                                          tCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGctc                                                                                 >  7:754624/1‑140 (MQ=255)

                                                                 tgtcaCTACGTGGTGAAACCCAGCGTCGGTCACAGCCGCGATTTGCCGACCTGTGGCTCAACTCCA‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                          <  2:1302/136‑1 (MQ=255)

                                                                 ctgcccTACGGGGGGAAAGCCCGCGGCGGTCCAATCCGGGTTGTGCCGACCAGTGGCACAACGGCC‑AAAAAGCGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCGCTCGTCaa                                                                          <  7:795500/135‑1 (MQ=255)

                                                                 cgTGCCTACGTGTTTAAATCCAGCGGCGTGAACATCCGCGTTTTGCCGACCAGGGGCTCAACTGCA‑AAAAAGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                          <  5:352487/139‑1 (MQ=255)

                                                                 cgTGACTAGGTGGTGAAATCCCGGGGCGGTCAAATCCGCGATTTGCCGACCAGTGGCACAAGTCCA‑AAAAAGCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                          <  3:917737/139‑1 (MQ=255)

                                                                 aGTTACTAGGTGGTGAAATCCCGCGGCGGTCACATCCGCGATTTGCCGCCCAGTGGCTCAACTCCC‑AAAAAGCGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                          <  1:526238/140‑1 (MQ=255)

                                                                 aGTGACTCGGTGGTCACCTCCCGCGTGGGGAACAGCCGCGATTTGCCGCCCAGGGGCGCAACAGCA‑AAAATGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                          <  8:452796/140‑1 (MQ=255)

                                                                   tGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAcc                                                                        >  3:663068/1‑140 (MQ=255)

                                                                   tGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAcc                                                                        >  3:66836/1‑140 (MQ=255)

                                                                   tGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAcc                                                                        >  5:268867/1‑140 (MQ=255)

                                                                     aCTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCATCCGt                                                                      >  6:929858/1‑140 (MQ=255)

                                                                     aCTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGt                                                                      >  5:57504/1‑140 (MQ=255)

                                                                       taggtggttAAATCCAGCGTCGGTCACATCCGCTATTTGCCGACCAGTGGCTCAAATGCA‑AAAAAGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                    <  4:651002/137‑1 (MQ=255)

                                                                       taggtggtgAAAGCCAGGGTCGGTCACATCCGCGATTTGCCCACCAGTTGCTAAACTGCA‑AAAACGCCTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                    <  8:829197/137‑1 (MQ=255)

                                                                       caCGTGGTTAAACCCCGCGGCGGTCACATCCGCGATTTGCCGACCAGGGGCTCAACTCCC‑AAAAAGAGCGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                    <  2:928606/139‑1 (MQ=255)

                                                                        aCGTGGTGAAATCCAGCGTCGGTCACATCCGCTATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTATACCTCCACCAAGACGGCTAAAAAGCCTAAAAATGATCAACGTGGCGCTCTCGTCAACCGTAtt                                                                   >  2:716363/1‑139 (MQ=255)

                                                                         cGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGCCTCTCCCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATgg                                                                  >  7:185106/1‑140 (MQ=255)

                                                                         cGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATgg                                                                  >  1:845754/1‑140 (MQ=255)

                                                                         cGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGACTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATgg                                                                  >  8:622752/1‑140 (MQ=255)

                                                                             gtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGCCTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGt                                                              >  2:519018/1‑140 (MQ=255)

                                                                             gtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATTAACGAGGCGCTCTCGTCAACCGTATTGGGGt                                                              >  3:523701/1‑140 (MQ=255)

                                                                             gtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACATCCACCAAGACGGCTAAAAAGCCTAAAAAGTATGAACGTGGCGCTCTCGTCAACCGTATGGGGGt                                                              >  8:60769/1‑140 (MQ=255)

                                                                                   tCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCGAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAGGGGGGTTGAccc                                                        >  4:290470/1‑140 (MQ=255)

                                                                                    ccAGCGGCGTTCACTACCGCGTTTTGCCGACCTGGGGCTAAATTGCA‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCg                                                       <  5:107594/140‑1 (MQ=255)

                                                                                       gggTCGGTAACATCCGCGATTTGCCGACCTGTGGCCCAACTGCA‑AAAAAGAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                    <  1:489217/138‑1 (MQ=255)

                                                                                       gCGTCGGTCACAGCCGCGTTTTGCCGACCAGTGGCTCAACTCCA‑AAAACGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                    <  1:618480/140‑1 (MQ=255)

                                                                                        cGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGc                                                   >  3:843952/1‑140 (MQ=255)

                                                                                        cGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGc                                                   >  7:526278/1‑140 (MQ=255)

                                                                                        cGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGc                                                   >  6:312429/1‑140 (MQ=255)

                                                                                        cGTCGGTCACATCCGCGATTTGCAGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGc                                                   >  4:955646/1‑140 (MQ=255)

                                                                                         gtaggTCACATCCGCTTTTTGCCGACAAGGGGCTCAACAGAC‑AAAACTAGACCCGACTCTACCTCCACCAAGACGTCTAAAAATCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGca                                                  <  5:923051/137‑1 (MQ=255)

                                                                                             gTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAAt                                              >  4:156563/1‑140 (MQ=255)

                                                                                             gTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCAACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACAGTATGGGGGTTGACCCGTGGCCCAAt                                              >  5:256865/1‑140 (MQ=255)

                                                                                                  aTCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACTTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGa                                         >  4:651124/1‑140 (MQ=255)

                                                                                                   cccGCGATTTCCCGCCCTGTGGCGCAAGTGCA‑AAAACGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAg                                        <  8:769911/139‑1 (MQ=255)

                                                                                                   accgggttttGCCGACCAGTGGCTAAACTGCC‑AAAAAGCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAg                                        <  8:1629/133‑1 (MQ=255)

                                                                                                     cgctATTTGCCGACCAGTGGCTAACCTGCA‑AAAACGCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAATGATGAACGGGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                      <  4:504984/140‑1 (MQ=255)

                                                                                                     cgcgTTTCGCCCACCCGTGGCTCAACTGCC‑AAAAAGAGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                      <  2:989998/140‑1 (MQ=255)

                                                                                                      gctaTTTGCCGACCTGTGGCTAAAGTGCC‑AAAAAGTGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                                     <  7:29797/137‑1 (MQ=255)

                                                                                                      gctaTTTGCCGACCAGTGGCTCAACTGAC‑AAAACGCGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                                     <  5:173399/137‑1 (MQ=255)

                                                                                                      gctaTTTGCCGACCAGGGGCTCAACTCCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                                     <  2:642637/137‑1 (MQ=255)

                                                                                                      gcgATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                                     <  3:55044/140‑1 (MQ=255)

                                                                                                      gcgATTTGCCGACCAGTGGC‑AAACCGCCAAAAAATCGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCGCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                                     <  5:171863/140‑1 (MQ=255)

                                                                                                       cgATTTGCCCACCAGTGGCTCACCTGCC‑AAAAATCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                                    <  4:425634/140‑1 (MQ=255)

                                                                                                       cgATTTCCCTACCTGTGGCTAAACTGCA‑AAAAACGGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                                    <  7:455295/140‑1 (MQ=255)

                                                                                                        taTTTGCCGACCTGTGCCTAAACTGCC‑AAAACGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                                   <  7:890038/139‑1 (MQ=255)

                                                                                                        taTTTGCCGACCAGTGGCTCAACTCCC‑AAAACGCGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                                   <  2:835450/139‑1 (MQ=255)

                                                                                                        taTTTGCCGACCAGTGGCTAAACTGCA‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                                   <  1:343126/139‑1 (MQ=255)

                                                                                                          tttGCCCACCAGGGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACt                                 <  7:902623/140‑1 (MQ=255)

                                                                                                            tGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAt                               >  7:491677/1‑140 (MQ=255)

                                                                                                                  cctgTGGCTAAACTGCC‑AAATAGAGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATAGGGAGGCGCACTATGAAgtg                         <  1:744206/137‑1 (MQ=255)

                                                                                                                      tGGCTAAACTGCC‑AAAATGAGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGc                     <  4:878973/140‑1 (MQ=255)

                                                                                                                      ggccTCACCTGCT‑AAAACGTGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCTCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGc                     <  4:721810/137‑1 (MQ=255)

                                                                                                                       ggCTCACCTGCC‑AAACCGAGTGCCGACTCTACCTCCACCAAGACTGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGGCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGcc                    <  5:543504/140‑1 (MQ=255)

                                                                                                                       ggCTCACCTGCA‑AAAATGCTGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGcc                    <  2:845754/140‑1 (MQ=255)

                                                                                                                       ggCTCAACTGCA‑AAAAAGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGcc                    <  4:800745/140‑1 (MQ=255)

                                                                                                                       gccgaaaCTGCC‑AAAAAGCGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGcc                    <  1:610529/135‑1 (MQ=255)

                                                                                                                        gCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCACCCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGccg                   >  8:369354/1‑139 (MQ=255)

                                                                                                                            aaCTGCC‑AAAAAGTGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTa               <  1:349902/140‑1 (MQ=255)

                                                                                                                            aaCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTa               >  5:144095/1‑140 (MQ=255)

                                                                                                                             aCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGATaa              >  1:591157/1‑140 (MQ=255)

                                                                                                                                 cc‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAagag          <  3:6813/140‑1 (MQ=255)

                                                                                                                                  aaaaaaaGAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAagag          <  7:172617/138‑1 (MQ=255)

                                                                                                                                       aaGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGgt     <  4:346175/140‑1 (MQ=255)

                                                                                                                                          agTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGgtcgt  >  2:532066/1‑140 (MQ=255)

TGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGT  >  NZ_CP022686/4951408‑4951684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A