Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F36 I0 R1 107 8366.7 6299831 98.3% 6192733 135.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→G 18.9% Q797Q (CAA→CAG ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AG18.9% 120.3 / 20.2 113Q797Q (CAA→CAGftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (36/41);  new base G (15/2);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 2.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATG  >  NZ_CP022686/4440197‑4440472
                                                                                                                                         |                                                                                                                                          
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACa                                                                                                                                          >  7:32407/1‑140 (MQ=255)
 tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCTCAGCCGCAGTATCAGCAGCGGCAAAAGCACCAg                                                                                                                                         >  6:724884/1‑140 (MQ=18)
  aCAGCAGCCGCACCAACCGGTAGCACCGCAGCCGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGTTTGAACCACAGCCGCAGTCTCCACTGCCGCAAGATCAGCa                                                                                                                                          >  2:783678/1‑138 (MQ=14)
  aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAACa                                                                                                                                          >  1:696012/1‑138 (MQ=255)
  aCAGCAGCAGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                                        <  5:350707/140‑1 (MQ=255)
      cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACATCCGGTAGCACAGCAGCCGCAGTATCAGCAGCAGCAACACCCGGTTCCACCAAATCCGCAGGATCCGCTGACGCAAAATCCTCAGCCGca                                                                                                                                    >  6:373164/1‑140 (MQ=14)
      cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGca                                                                                                                                    <  8:202190/140‑1 (MQ=255)
       agcCGCAACAACCGGTAGCACCGCAGCAGCATTATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                                   <  5:1601/140‑1 (MQ=255)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAACAACCGGTTGCTCCTCAGCCGCAGTATCAGCTGCAGCAATATCAGCAGACGcc                                                                                                                                    >  3:75075/1‑137 (MQ=18)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAACAGAAACAACCCGATGCACCCCAGCCGCAGTAGCACAAGCCGCAAGATAAACAACCGcaac                                                                                                                                  >  5:23758/1‑140 (MQ=18)
         ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGAAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGATGCACATCACCCGCAGTGTCAGCAGCCGCAAAATCATCAGCCGccacc                                                                                                                                 >  5:211409/1‑139 (MQ=18)
         cagCATCAACCGTTAGCAACGCAGCAGAAATATCAGCAGCAGCAACAACCGGTATCTCCTCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                 <  5:501622/138‑1 (MQ=18)
          cGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCCGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCTGCCGCAATATCAACAGCCGcaac                                                                                                                                  >  3:199770/1‑138 (MQ=255)
           gCAACAACCGGTAGCACCGCCGCAGCAATATCAGCAGACGAAACAACCGGTAGCACCGCCGCAGCAGTATCAGAAGCCTCAACATCAGCTTTCACAGCAGCCGAAATAACAGCATAATCAAAATCATCTGCCCcaacagc                                                                                                                               >  6:26263/1‑138 (MQ=9)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTGGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCGCCCCAGCCGCAGTATCAGCAGCCGCAAGAGCAGCAGACGcaacagc                                                                                                                               >  4:110670/1‑136 (MQ=18)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATAAGCAGCCGCAATATCCACAGCCGcaacagc                                                                                                                               >  3:95795/1‑136 (MQ=21)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACAACCGGATGAACCTCATCCGGAGAATCAGCAGCAGCAATATCAGCAGCCGccacacc                                                                                                                               >  4:635291/1‑136 (MQ=18)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAACACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCACAGCCGCAGGATACGCAGCCGCAATCTCGGCAGACGCaaaaac                                                                                                                               >  2:428969/1‑138 (MQ=14)
                       aGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCTCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCAGCAACATCCGGATGCAcc                                                                                                                     >  2:42251/1‑138 (MQ=18)
                        gCACCGCAGCAGCAATATCAGCAGCCTCAACATGCGGTAGAACCGCAGCCGCATTATCAGGAGCCGCAACAACCGGTTGCACCGCAGCAGCAGTATCATCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCg                                                                                                                    <  3:2737/138‑1 (MQ=25)
                        ccACCGCAGCAGCAATCTCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGGAACAACCGGTTGCACCGCAGCCGCAGTAACATCATCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                                  <  7:107229/139‑1 (MQ=25)
                        ccACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTATCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                                  <  5:31096/139‑1 (MQ=255)
                                gcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACACCCGGTTGCACCgcagcagca                                                                                                            >  1:814668/1‑138 (MQ=255)
                                    caATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCCCCTCAGCCGCAGGAGCCGCAGCAGCATAATCAGCAGCCGCCACAACCGGATGGACCGCAGCTGCAAAATcc                                                                                                      >  8:127782/1‑139 (MQ=17)
                                               gcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCTCCGCAGCCGCAGTATCGGCAGCGGCATTATCAGCAGCCGCCACAACCGGATGCACCGCGGCAGCAATATCAgccgcccccac                                                                                           >  5:633886/1‑135 (MQ=18)
                                               gcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCCGCAATATCACCAGCCGCCACACCCGGTTGCGCCGCAGCAGCAATATCAgccgccccc                                                                                             >  1:266393/1‑135 (MQ=21)
                                                ccGCTACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                          <  7:398222/140‑1 (MQ=255)
                                                ccGCAACAACCGGTAGCTCCGCAGCAGCAGTATAAGCAGCCGCAACAACCGGTTGCACCGCATCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                            <  4:650499/138‑1 (MQ=255)
                                                ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                            <  3:507174/138‑1 (MQ=255)
                                                ccGCAACAACCGGCAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                          <  6:149622/140‑1 (MQ=255)
                                                ccGCAACAACAGGTAGCTCCGCAGCCTCAGTATCAGCAGGCTCATCATCCGGTTGCACCGCTGCCGCAGTAACAGCATCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                          <  6:708487/140‑1 (MQ=25)
                                                  gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCATCAGCAGCAACAGCCGGTTGCGCCGCAGCCGCTATCTCAGCAGCaacaacc                                                                                          >  4:507173/1‑137 (MQ=18)
                                                  gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                        <  7:157812/140‑1 (MQ=255)
                                                   caacaaCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGAACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCCCCTCAGCAGCAATATCAGCCGCCCCAACAg                                                                                         >  3:777654/1‑138 (MQ=21)
                                                      catccGGAAGCAACGCAGCCGAAGTAGCAGCAGCCTCAACAACCGGATGCACCGCAGCATAAGTATCAGGAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCg                                                                                      <  4:380273/135‑1 (MQ=25)
                                                            ctAGCACCGCAGCCGCAGTATCAGCAGCATAAACAACAGTATCAACAACAGCCGAAGTATAAGCAGCCGCAAAATAAGAATACAAAACAACCGGATGCAACGCAGCAACAAAATAAGAAGCCACAACAGCCggggccc                                                                                >  2:199953/2‑133 (MQ=255)
                                                             aaGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTCGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                               <  3:249889/137‑1 (MQ=255)
                                                                cACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagc                                                                            >  4:665710/1‑138 (MQ=255)
                                                                cACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCAAGATCACCAGCCGCAACAACCTGTGGCTCCGCAGCAGCAATATAAGCCGCCAGCACCGCCGGTTaccccgc                                                                            >  3:430572/1‑133 (MQ=21)
                                                                  ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                        >  7:535397/1‑140 (MQ=255)
                                                                  ccgcagccgcagTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCCGTTacaaaccac                                                                          >  4:130335/1‑132 (MQ=37)
                                                                  ccgcagccgcagGCTCAGCAGCCGCAACAACCGCGTGCACCGCAGCCGCCGGATCAGCAGCAGCAATCTCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGcc                                                                        <  8:604561/140‑1 (MQ=25)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCCACAGCCGCAACAACCGGATGAACCGCAGCAGCAATATCCGCAGCCCCCACAGCCGGTTAACCagcagcag                                                                       >  1:328128/1‑136 (MQ=25)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACCACCGGTTGCACCTCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCa                                                                     >  7:670026/1‑140 (MQ=255)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATAAACTGCCTCAACAACCGGATGCACCCCAGCATCAATATAATCATGAACAACATCCGCTGACAaaccagaaa                                                                       >  4:294278/1‑135 (MQ=12)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                     >  4:403963/1‑138 (MQ=255)
                                                                             gTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCATTATCAGCAGCCGCCTCATCCGGTAGCACCGCAGCAGCATAATCAGCAGCCCCAACAGCCGGTTAAACAGCAGCCGCAGGATcc                                                               >  4:339847/1‑137 (MQ=21)
                                                                             gTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGAATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCGGCAATCTCAGCAGCCACAGCAGCCGGTTCCACAGCCTCAGCAGTATcagc                                                             >  5:340107/1‑140 (MQ=25)
                                                                                  agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCTCAATATCAGCAGCCGCACCAACCGGTAGCCCCGCAGAAGCAATATCAGCCGCCGCCACAGCCGGGTAGCCATCAGCAGCAGGATccgccgccg                                                        >  8:263382/1‑140 (MQ=19)
                                                                                    cagcCGCAACACCCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCGGTATCAGCAGCCg                                                        <  3:106110/138‑1 (MQ=255)
                                                                                         gCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGTCGCAGTATCAGCAGCCGCAACa                                                   <  1:807666/138‑1 (MQ=255)
                                                                                          caacacCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTCCACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                  >  3:789682/1‑138 (MQ=255)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGATGCAATATCTCCGGCCCCAACAGGCGGTTCAAACGAAGCCGCTGAATCAGCAGACGCaaaggcc                                                >  6:342304/1‑135 (MQ=21)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                  >  1:554782/1‑138 (MQ=255)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                                >  6:55633/1‑140 (MQ=255)
                                                                                                     tGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCCACAGCCGCAACAACCGGTTGCACCGCAGCCGCAATAGCAGCAGCCACAGACGCCGGTTAGCCAGCACCCGCAGGAGCCGCAGCCGCAACACCCGGATGTaacgc                                     >  6:408312/1‑136 (MQ=21)
                                                                                                     tGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcc                                       <  1:24081/138‑1 (MQ=255)
                                                                                                     ggaccagcagcagcatTAGCAGCATCACCAAGATCACCAGCCGCAACCACCGGGTGCACCGCAGCAGCAATCTCACCAGCCACAACAGCCGGTTACACAGCAGCAGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                     <  6:190257/134‑1 (MQ=18)
                                                                                                       ccccgcagcggcagCCTCAGCAGCCGCAAGCTCCACAGCCGCAACACCAGTGTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                   <  8:337507/138‑1 (MQ=18)
                                                                                                         ccgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                   <  2:554782/138‑1 (MQ=255)
                                                                                                         ccgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                 <  8:32407/140‑1 (MQ=255)
                                                                                                         ccgcagccgcagGCTCTGCAGCAGCAATATCAACAGCCGCAACAACCGCTAGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                   <  4:731212/138‑1 (MQ=18)
                                                                                                          cgcagcctcagTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCTCAACAGCCGGTTGTACCACAGCCg                                <  8:481492/140‑1 (MQ=255)
                                                                                                            cagccgcagTATCAGCCGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                              >  5:700653/1‑140 (MQ=255)
                                                                                                              gcgtcagAATCAGCAGCAGCAATATCACCAGCCGCAACAACCGCTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                            <  8:310116/136‑1 (MQ=21)
                                                                                                              gccgcagTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCCCCGCAGCAGCAAGATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCCACAGCCGGATGTACCACAGCCGCa                              >  3:12789/1‑138 (MQ=21)
                                                                                                              gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                            <  7:702819/140‑1 (MQ=255)
                                                                                                               ccgcagGCTCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAg                             <  1:305177/138‑1 (MQ=255)
                                                                                                                   tttATCTGCAGCCGAAATAGCCACAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                         <  3:294279/136‑1 (MQ=18)
                                                                                                                   attATCAGCAGCATCAATAACAACAGCCTCAACAACATGATTCACATCAGCAGCAATTACAGCAGCCACAACATCCGGTTACACAGCAGCAGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                       <  6:627171/138‑1 (MQ=18)
                                                                                                                   agaaTCAGCAGCAGCATTAACAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGGTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                         <  4:576682/135‑1 (MQ=18)
                                                                                                                   agTCTCTGCTGCCTCATTATCAACAGGCGAAACAACCGGGTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                       <  6:708980/140‑1 (MQ=18)
                                                                                                                    gTATCAGCAGCCGCAATATCTTCAGCCGCACCAACCGGTGGCACCTCAGCAGCAATCTCAGCTGCCACAACAGCCGGGTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc                        <  1:199953/138‑1 (MQ=21)
                                                                                                                      aTCAGCAGCCGCAAAATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGTTTACAAACAAGCCGCAGTATCTGCAGCCGCACCAGCCGGTTGTACCACCGCCGCAGGATAcgc                      >  1:117025/1‑136 (MQ=21)
                                                                                                                         agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTCCCACAGCAGCAGGATACCCTGCtt                 >  7:747195/1‑140 (MQ=255)
                                                                                                                         agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt                 >  6:437702/1‑140 (MQ=255)
                                                                                                                         agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTAACAGCAGCCGCAACAGCCGGTGGTACCACAGCCGCAGGATCCCCTGCta                 >  6:414707/1‑139 (MQ=255)
                                                                                                                          gcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                                  <  8:334908/42‑1 (MQ=255)
                                                                                                                          gcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                                  >  7:334908/1‑42 (MQ=255)
                                                                                                                          gcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTAACAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTc                >  6:258992/1‑140 (MQ=255)
                                                                                                                             tgagCAATTACATCATCCGCAACAACCGGCTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTCCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc             <  8:532107/137‑1 (MQ=18)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATCCCCTGCTTCATc             >  8:38625/1‑140 (MQ=255)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCAACGCACCAGCAATATCAGCAGCCACAACAGCCGGTTACACACCAGCCGCAGTATCAGCATCCGCAACAGCCGTTGGTAACACAGCCCCAGGATACCATGCGTCATc             >  7:171872/1‑140 (MQ=37)
                                                                                                                              ccGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                   <  8:659231/117‑1 (MQ=255)
                                                                                                                              ccGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                   >  7:659231/1‑117 (MQ=255)
                                                                                                                               cGCAATATCATCAGCCGCATCACCCGGTTGAACCGCAGCAGCATTATCAGAAGCCACAACAGACGGTTTCACAGCAGCCTCAGTATCAGCAGCCGCAACAGCCGGTTGTAACACAGCCGCAGGATACCCTGCTTCATCCg           <  8:512887/140‑1 (MQ=21)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATCCCCTGCTtcctccc           >  6:44320/1‑139 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAAAGCCTGTTGTACCACAGACGCAGGATAACCTGCTTCAta             >  3:621350/1‑137 (MQ=21)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACCGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg           >  7:663254/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg           <  5:284508/140‑1 (MQ=255)
                                                                                                                                 cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCCGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGCATACCCTGCTTCATCCGCt         >  8:645942/1‑140 (MQ=255)
                                                                                                                                 cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATcca           >  2:799607/1‑137 (MQ=255)
                                                                                                                                   atatCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACGCTGCTTCATCCGCt         >  3:515209/1‑138 (MQ=255)
                                                                                                                                   atatCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACGCTGCTTCATCCGCTGt       >  6:347171/1‑140 (MQ=255)
                                                                                                                                     ttCAACAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt       <  4:91092/137‑1 (MQ=255)
                                                                                                                                     atCCACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt       <  4:5115/138‑1 (MQ=255)
                                                                                                                                     atCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCATCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt       <  3:18947/138‑1 (MQ=255)
                                                                                                                                     atCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt       <  4:674761/138‑1 (MQ=255)
                                                                                                                                       cAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg     <  4:733349/138‑1 (MQ=255)
                                                                                                                                        aaCAGCCGCACCAACAGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGAAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGAGACCCTGCTTCATCCGTTGTTGATg  >  6:180934/1‑140 (MQ=21)
                                                                                                                                         |                                                                                                                                          
GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATG  >  NZ_CP022686/4440197‑4440472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A