Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F35 I0 R1	195	10216.4	8585726	98.3%	8439768	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,769,186	C→A	16.0%	M50I (ATG→ATT)	rarD ←	EamA family transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,769,186	0	C	A	16.0%	241.0 / 9.1	122	M50I (ATG→ATT)	rarD	EamA family transporter RarD
Reads supporting (aligned to +/- strand): ref base C (70/28); new base A (2/22); total (72/50)
Fisher's exact test for biased strand distribution p-value = 1.39e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT  >  NZ_CP022686/2769049‑2769324

ttGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCCGCAC‑CACCAt                                                                                                                                          >  2:87105/1‑140 (MQ=255)

ttGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCAt                                                                                                                                          >  1:566030/1‑140 (MQ=255)

gtGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCCCGGCAGAGACTGCCAGCATTAAAATTTTATGTGGCGTCTGAATCAGCGTTTTTAAAGAGGACCACTGGCGGCACATGCTCATCGGCCA‑CACCAt                                                                                                                                          >  8:645774/2‑140 (MQ=255)

 tGTTTACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGAGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCTGAAAATGCTCATCAGCCC‑CCCAATa                                                                                                                                         >  2:1109394/1‑140 (MQ=255)

 tGTTCACCGCCCAGATAACCAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGGGGCGTCTGAAGCAGCGTTTTTTAATAGGACCACTGGCGGCACATGCTCAGCCGCac‑caccc                                                                                                                                           >  5:209210/1‑137 (MQ=255)

  gTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGCCCACTGGCGGCAAATGCTCATCAGCAC‑CACCAt                                                                                                                                          >  5:621057/1‑138 (MQ=255)

  gTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCAt                                                                                                                                          >  6:237564/1‑138 (MQ=255)

   ttCACCGCCAAGATAAACAGTAGCCAGTTGCCGCCATTCATCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATAAGCAC‑CCCCATaaa                                                                                                                                       >  8:630992/1‑140 (MQ=255)

     cACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGa                                                                                                                                     >  4:391123/1‑140 (MQ=255)

       ccGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGa                                                                                                                                     >  6:379475/1‑138 (MQ=255)

             aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGAc                                                                                                                             >  4:321786/1‑140 (MQ=255)

             aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGAc                                                                                                                             >  8:53499/1‑140 (MQ=255)

               aTAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTCGCGACTGAATCAGCGTGTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACgcc                                                                                                                             >  5:445848/1‑136 (MQ=255)

                  aaCAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CCCCATAAAGAAAAACGCCCAGAt                                                                                                                        >  1:76665/1‑140 (MQ=255)

                     aGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCAc                                                                                                                     >  2:42202/1‑140 (MQ=255)

                       tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCCGCAC‑CCCCATAAAGAAAACCGACCAGATCAcgc                                                                                                                   >  8:908914/1‑140 (MQ=255)

                        aGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCAcgcg                                                                                                                  >  1:630171/1‑140 (MQ=255)

                         gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAATAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAACGACAAACGCCCAGATCAcgc                                                                                                                   >  6:597673/1‑138 (MQ=255)

                         gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGGTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                 >  7:948661/1‑140 (MQ=255)

                         gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCCTAAAGAAAAACGACCAGATCACGCGa                                                                                                                 >  3:589227/1‑140 (MQ=255)

                         gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                 >  4:116628/1‑140 (MQ=255)

                         gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGACAGCATAAAAATTTTCTTTGGCGTCTGAATCAGCCTTTTTAAATAGGACCACTGGCGGAAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATcctctct                                                                                                                 >  3:479927/1‑134 (MQ=255)

                          cctgTTGCCCCCAATCCCCCCGGCATTTACTGCCAGAATAAAAATTTTGGGTGGGGTCTTTATCAGGGTTTTTAAATTGGACCACGGGCGGAAAATGCTCAGAAGCAC‑CACAATAAATAAAAACGACCAGATCAcgcg                                                                                                                  <  6:504002/135‑1 (MQ=255)

                          ccAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCAcgcg                                                                                                                  >  5:25285/1‑138 (MQ=255)

                          ccAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCAcgcg                                                                                                                  >  6:75449/1‑138 (MQ=255)

                             tttcccgccAATCAGCACGCCAGATACTGCCCGCATAAAAATTTTCTGTGGCGTCTTAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCACCAC‑CACAATAAAGAAAAACGACCAGATCACGCGATGCg                                                                                                             <  4:621111/136‑1 (MQ=255)

                              ttGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGt                                                                                                            >  3:868340/1‑140 (MQ=255)

                                 ccgccAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGCTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTc                                                                                                           >  6:6835/1‑138 (MQ=255)

                                 ccgccAATCACCCCGGCAGAGACGCCCAGCAAAAAATTTTTATTGGGCGTCTGAATCAGCTTTTTTAAATCCCACCACTGGCGCAATATGCTCATCAGAAC‑CAAAATAAAGAAAAACGACCAGATCACGCGATGCGTCAg                                                                                                         <  4:875235/140‑1 (MQ=255)

                                     cAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAggc                                                                                                       >  5:85592/1‑137 (MQ=255)

                                            cACGGCAGAGACTGCCAGCTTAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATcgg                                                                                              >  1:999358/1‑140 (MQ=255)

                                            cACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CCCCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGAACTCATcgg                                                                                              >  7:783281/1‑140 (MQ=255)

                                             agggCATACACTGCCAGCATAAAAATTTTCTGGGGGGTCTTAATCAGCGTTTTAAAATAGCCCCACTGGCGTCAATTGCTCATCAGCAC‑CACCATAACTAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATcggc                                                                                             <  8:493041/138‑1 (MQ=255)

                                              cGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGCCCAGATCACGCGATGCGTCAGGATCTCATcgg                                                                                              >  5:926527/1‑138 (MQ=255)

                                                 cAGAGACTGCCAGCATAAAATTTTTCTGTGGCGTCTGAATCATCGTTTTTAAATAGGACCACTGGCGGCAAAAGCTCATCAGCAC‑CACAATAAAGAAAAACGACCTGATCACGCGATTCGTCAGGATCTCATaggcggtc                                                                                         >  1:119486/1‑138 (MQ=255)

                                                      acagCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACg                                                                                      >  5:1027277/4‑138 (MQ=255)

                                                      aCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgta                                                                                    >  2:410918/1‑140 (MQ=255)

                                                         gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCCGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                 >  1:759865/1‑140 (MQ=255)

                                                         gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACggagta                                                                                 >  3:425842/1‑140 (MQ=255)

                                                          ccAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaa                                                                                >  7:813571/1‑140 (MQ=255)

                                                          ccAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCAACAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGCTCTCATCGGCGGGCACGTAGTaa                                                                                >  1:189466/1‑140 (MQ=255)

                                                           cAGAATAAAATTTTTCTGGGGCGTCGTAATCAGCGTTTTTAAATCGGACCACTGGCGCCAAATGCTAATCAGCAC‑CACAAAAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                               <  2:299632/140‑1 (MQ=255)

                                                            cgaaTAAAAATTTTCGGGGGCGTCTTCATCTGCTTTTTAAAATCGCACCACGGGCGGAAAATGCTCATCAGCAC‑CACCAAAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAAt                                                                              <  2:692379/137‑1 (MQ=255)

                                                            agaaTAAAAATTTTGTGGGGCTCGTGAATCCGTGTTTTTAAATCGGCCCAGTGGGGGAAAATGCTAATCAGCAC‑CACAATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAAt                                                                              <  7:730209/137‑1 (MQ=255)

                                                                tAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGt                                                                          >  8:1017403/1‑140 (MQ=255)

                                                                  aaaaTTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGttt                                                                        >  2:1101908/1‑140 (MQ=255)

                                                                  aaaaTTTTCTGTGGCGTCTGAATAAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGatt                                                                        >  2:207330/1‑140 (MQ=255)

                                                                     aTTTTCTGGGGCGGCTGAATCAGCGTTTTAAAATAGCACCACTGGCGCCAATGGCTCATCCCCAC‑CACCATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGaa                                                                     <  2:210729/140‑1 (MQ=255)

                                                                         tCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTAGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGTCACGTAGTAAATCAGTTTGAAGTAc                                                                 >  8:1008040/1‑140 (MQ=255)

                                                                           tgtgGCGTATTATTCTGCGTTTTTAAATAGCACAAGTGGCGGCAAATGCTCATCAGCAC‑CACAATAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGc                                                               <  7:237569/140‑1 (MQ=255)

                                                                           tggggCGTCTGAATCCGCGTTTTAAAATAGGCCCACTGGCGGCAAATGCTCATCCGCAC‑AACCATAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGc                                                               <  2:321124/137‑1 (MQ=255)

                                                                                  tattaaTCAGCGTTTTAAAATCGCCCCACTGGCGCAAATTGCTAATCACCCC‑CACCAAAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCa                                                        <  7:537256/136‑1 (MQ=255)

                                                                                  tCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCa                                                        >  1:1042962/1‑140 (MQ=255)

                                                                                  gcTGAATCAGCGTTTTTAAATAGCCCCACGGGCGCAAAATGCTCATCACCAC‑CACCAAAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCa                                                        <  2:772106/139‑1 (MQ=255)

                                                                                   cttaaTCAGCTTTTTAAAATAGCACCACTGGCGCAAAATGCTAATCCCCAC‑CACAAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCaa                                                       <  3:391123/137‑1 (MQ=255)

                                                                                       aTCAGGGTTTTTAAATTGATCCACTGTCGGAAATTGTTCATCAGCAC‑CACATTAAATAAACACGACCAGATCACGCTATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATa                                                     <  6:170194/138‑1 (MQ=255)

                                                                                          aGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCCGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCCTAAGGATATCATCGGCGGGCACGGAGTAAATCAGTTTGAAGTACGCTGGCGCAATCCCCCa                                                >  8:663204/1‑140 (MQ=255)

                                                                                            tgTTTTTAAATCGCCCCCCTGGCGTAAAATTCACAACAGCAC‑CACCAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCa                                                <  6:25285/137‑1 (MQ=255)

                                                                                            cGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATAGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaa                                              >  3:475863/1‑140 (MQ=255)

                                                                                              tttttAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaa                                              >  6:631228/1‑138 (MQ=255)

                                                                                               ttttAAATAGTCCCCCTGGCGGAAAATGCTAATCACCCC‑CCCCATAAACAAAAACGACCAGATCACGCGATGCGTCAGTATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataa                                           <  8:694869/140‑1 (MQ=255)

                                                                                                tttAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                          >  3:836134/1‑140 (MQ=255)

                                                                                                tttAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                          >  4:550531/1‑140 (MQ=255)

                                                                                                tttAAATAGGACCACTGGCGGCAAATGCTCATAAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTAATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACCGTGGCGCAATACCCCaaataaa                                          >  7:457086/1‑140 (MQ=255)

                                                                                                  tAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                          >  5:821870/1‑138 (MQ=255)

                                                                                                  tAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                          >  6:622864/1‑138 (MQ=255)

                                                                                                   aaaatggACCACTGGCGTAAATTGTTCATCTGCAC‑CACAATAATTAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaata                                       <  2:363987/135‑1 (MQ=255)

                                                                                                   aaaTTGGACCTCTGGTGCATATTGCTCATACCCAC‑AACATTAAAAAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaa                                         <  6:401449/138‑1 (MQ=255)

                                                                                                   aaaTAGGACCACTGGCGTAAAATGCTCATAACCAC‑CACCAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaata                                       <  2:713328/140‑1 (MQ=255)

                                                                                                         gACCACTGGCTGCAAATGCTACTC‑CCACACACCAAAAATAAACACGACCAGATAACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGTCGCAATACCCCCAATAAAATCAGCGGc                                 <  4:378402/140‑1 (MQ=255)

                                                                                                         gACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGCCCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                 >  8:688257/1‑140 (MQ=255)

                                                                                                          aCCACTGGCGGAAAATGCTCATCAGCAC‑CACCAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCgg                                  <  5:483424/138‑1 (MQ=255)

                                                                                                            cACTGGCTGAAAATGATCATCAGCAC‑AACAAAAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAag                              <  2:645703/140‑1 (MQ=255)

                                                                                                               tgccgccAAATGCTACTCACCAC‑CACCAAAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGc                           <  1:257556/134‑1 (MQ=255)

                                                                                                               tGGCGTCAATTGCGAATCATCAC‑AACAATAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAaga                             <  6:751260/138‑1 (MQ=255)

                                                                                                               tGGCGGAAATTGCTAATCAGCAC‑CACAAAAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGc                           <  2:244753/140‑1 (MQ=255)

                                                                                                               gggcgtcAATTTCTCAACTGCAC‑CACCTAAAAGAAATACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGc                           <  8:686333/139‑1 (MQ=255)

                                                                                                               gggcggaAAATGCGCATCACCAC‑CACAATACAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAaga                             <  5:535116/137‑1 (MQ=255)

                                                                                                               gggcgccAAATGCTCATCCCCCC‑CACCAAAACAAACAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGc                           <  2:12425/139‑1 (MQ=255)

                                                                                                                ggcgtaaaaTGCTCATCACCCC‑CCCCAAAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCg                          <  1:102231/134‑1 (MQ=255)

                                                                                                                ggcggcAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAATTAAGCGGCAagag                            >  6:1021450/1‑138 (MQ=255)

                                                                                                                ggcgcaaaaTGATCATCACCAC‑ACACATAAAAAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATACGCGGCAAGAGCg                          <  4:945899/134‑1 (MQ=255)

                                                                                                                 gcggcAAATGCTCATCAGCCC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCACTACCCCAAATAAAATAAGCGGCAAGAGCGa                         >  1:783536/1‑140 (MQ=255)

                                                                                                                 gcggcAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGa                         >  4:107178/1‑140 (MQ=255)

                                                                                                                     cAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGt                       >  6:304716/1‑138 (MQ=255)

                                                                                                                     cAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGt                       >  6:674569/1‑138 (MQ=255)

                                                                                                                      aattGCACATCAACAC‑AACAAAAAAAAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGAGCAAAACCCCAAATAAAATAACCGGCAAGAGCGAGTAAt                    <  7:554660/137‑1 (MQ=255)

                                                                                                                                 aGCAC‑CACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcg         <  4:461429/140‑1 (MQ=255)

                                                                                                                                 aGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGAACTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGAAATAGCTATTAATACGCCCtac           >  6:136991/1‑136 (MQ=255)

                                                                                                                                   cac‑cacCATAAAGAAAAACGACCAGATCACGCGATTCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGAAATACCCCAAATAAAATACTCGGCAAGAGAGAGTAATACGCACTGCcgcg       >  8:855107/1‑140 (MQ=255)

                                                                                                                                   cac‑cacCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcgcg       >  3:205476/1‑140 (MQ=255)

                                                                                                                                    cc‑caccaTAACCAACCACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt      <  3:346311/139‑1 (MQ=255)

                                                                                                                                    cc‑cacaaTAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt      <  1:453625/139‑1 (MQ=255)

                                                                                                                                     ccccccaAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt      <  1:224532/136‑1 (MQ=255)

                                                                                                                                       caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgttt    >  8:55381/1‑140 (MQ=255)

                                                                                                                                       caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtat    >  8:122555/1‑138 (MQ=255)

                                                                                                                                         ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt  >  2:1066282/1‑140 (MQ=255)

                                                                                                                                         ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt  >  7:89662/1‑140 (MQ=255)

                                                                                                                                         ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt  >  4:1066783/1‑140 (MQ=255)

                                                                                                                                         ccaTAAAGAAAAACGACCAGATCACGCGATGCGACAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCCCgttt    >  5:767365/1‑138 (MQ=255)

TTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCAC‑CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT  >  NZ_CP022686/2769049‑2769324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A