Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F13 I0 R1 230 5035.0 9125430 98.4% 8979423 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,769,196 A→C 16.3% F47C (TTT→TGT)  rarD ← EamA family transporter RarD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,769,1960AC16.3% 308.4 / 21.5 176F47C (TTT→TGT) rarDEamA family transporter RarD
Reads supporting (aligned to +/- strand):  ref base A (110/34);  new base C (3/29);  total (113/63)
Fisher's exact test for biased strand distribution p-value = 1.35e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT  >  NZ_CP022686/2769058‑2769335
                                                                                                                                           |                                                                                                                                           
gCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGaaaaa                                                                                                                                            >  5:789802/1‑140 (MQ=255)
gCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGaaaaa                                                                                                                                            >  5:1097542/1‑140 (MQ=255)
    aGATAAACAGTAGCCAGTTGCCGCCAATCAGAACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTATAATTAGGACCACTGGAGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  6:51987/1‑140 (MQ=255)
      aTAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  7:280528/1‑138 (MQ=255)
           cAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCa                                                                                                                                 >  1:157769/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCGAGCATATATATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCTGATCAcgc                                                                                                                              >  5:903792/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCCCCACCATAAAGAAAAACGACCAGATCAcgc                                                                                                                              >  2:80667/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCAcgc                                                                                                                              >  1:21748/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCAcgc                                                                                                                              >  3:287649/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGATCATCAGCACCACCATAAATAAAAACGACCAGATCAcgc                                                                                                                              >  1:961837/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTATTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCAcgc                                                                                                                              >  5:1001863/1‑140 (MQ=255)
              tAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGACTGAATCAGCGTTTTTAAATAGGCCCACTGGCGGCAAA‑TGCTCATCAGCACCACCAAAAAGAAAACCGACCAGATCAcgc                                                                                                                              >  4:831182/1‑140 (MQ=255)
                gCCAGTTGCCGCCAATCAGCACGGCAGAGCCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  5:465760/1‑140 (MQ=255)
                gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAATATTTTCTGTGGAGACTGCATCAGCGTATTTAAATACGACCACTGGCGGCAAA‑TGCGCATCAGCACCACCATAAAGAAAACAGACCACATCAAGCGa                                                                                                                            >  3:138464/1‑140 (MQ=255)
                gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATATAGAAAAACGACCAGATCACGCGa                                                                                                                            >  4:885749/1‑140 (MQ=255)
                gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAACACGACCAGATCAcgcgc                                                                                                                            >  5:664061/1‑139 (MQ=255)
                gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCAcgc                                                                                                                              >  8:308726/1‑138 (MQ=255)
                 ccAGTTGCCGCAAAGCAGCCCGGCATAGACTGCCAGAATATAAATTTTGTGTGGCGTCTGAATCCTCGTTTTTAAATAGGCCCACTGGCGGAAAT‑TGCTCATCAGCCCCACAATAAACAAACACGACCAGATCACGCGAt                                                                                                                           <  6:775731/140‑1 (MQ=255)
                  cAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGTACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  7:320796/1‑138 (MQ=255)
                  cAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  8:48391/1‑138 (MQ=255)
                  cAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATATTCAGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGAGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  8:24155/1‑138 (MQ=255)
                    tttGCCTCCAACCCGCACGCAAGACACGCCCATAATAAAATTTTTGTGGGGCGTTTTAACCTGTTTTTTTAAATCGGACCACGGGCGTAAAT‑TGCTAATCCCCACCACAAAAACCAAACACGACCAGATCACGCGATGCg                                                                                                                        <  2:157769/139‑1 (MQ=255)
                     ttGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCgg                                                                                                                       >  6:30389/1‑139 (MQ=255)
                     ttGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGt                                                                                                                       >  6:96980/1‑140 (MQ=255)
                        ccgccAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGCCCAGATCACGCGATGCGTCAg                                                                                                                    >  6:580453/1‑140 (MQ=255)
                          gccAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGa                                                                                                                  >  1:870335/1‑140 (MQ=255)
                            cAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGCCCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGCACAACGCCCAGATCACGCGATGAGTCAGGa                                                                                                                  >  7:137123/1‑138 (MQ=255)
                                   cACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATATGACCAATGGCGGCAAA‑TGCTCATCAGCACCCCCATAAAGAACAACCACCAGATACCGCGATGCGTCAGGATCTCATcgg                                                                                                         >  4:474412/1‑140 (MQ=255)
                                   cACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCCTCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGGCAGGATCTCATcgg                                                                                                         >  2:44062/1‑140 (MQ=255)
                                     cgtcatagaCTCCCTGAATAAAAATTTTCTGGGGCGTTTGAATCCGCTTTTTTAAATTGGACCAGTGGCGGAAAA‑TGCTCATCAGCCCCACCAAAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATcggcg                                                                                                       <  2:1085694/134‑1 (MQ=255)
                                      ggCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTATTAAATAGGACCACTGGCGGCAAA‑AGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATCCGTCAGGATCTCATaggcgg                                                                                                      >  3:99469/1‑140 (MQ=255)
                                        cAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGc                                                                                                    >  4:43900/1‑140 (MQ=255)
                                         ctagagTGACATCAAAAAAATTTCGTGTGTCGTCTGAATCAGCGTTTTAAACTAGTACCACGGGCGGAAAA‑TGCTCATCACCACAAACAAAAAAAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCa                                                                                                   <  3:847642/138‑1 (MQ=255)
                                         agagACTGCCAGAATAAAAATTTTCTTTGGCGTCTTAATCAGCGTTTTTAAATAGCACCCCTGGCGGAAAA‑TGCTAATCACCACCACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCa                                                                                                   <  4:120714/140‑1 (MQ=255)
                                           agaCGGCAAGCATAAAAATTTTCTGGGGCGTCTTAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACg                                                                                                 <  4:677044/140‑1 (MQ=255)
                                           acccTGCCAGAAAAAAATTTTGCTGTGGCGTCTTAAGCAGCGTTTATAAATCGCCCCACGGGCGTCAAT‑TGCACATCACCCCCCCAATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACg                                                                                                 <  5:805296/137‑1 (MQ=255)
                                             aCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATAGGCGGGCACgtt                                                                                               >  5:341468/1‑139 (MQ=255)
                                                gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGCCCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                            >  1:556806/1‑140 (MQ=255)
                                                gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                            >  3:37074/1‑140 (MQ=255)
                                                gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGATTATAAATAGGACCACTGGCGGCAAA‑TGCACATCAGCACCACCATAAAGAAAACCGACCATATCACGCGATGCGTCAGGATATCATCGGCGGGCACgtagta                                                                                            >  6:698995/1‑140 (MQ=255)
                                                 ccAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaa                                                                                           >  3:467238/1‑140 (MQ=255)
                                                  cacaaTAAAATTTTTCTTTGGGGTATCAATCAGCTTTTTTAAATAGCACCAGTGGCGTAAAA‑TGCTAATCAGCACCCCAATAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                                          <  3:1098890/136‑1 (MQ=255)
                                                  cAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                            >  7:963241/1‑138 (MQ=255)
                                                  cAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                                          >  3:759678/1‑140 (MQ=255)
                                                  aaGCATAAAAATTTTCTGTGGCGTCTTAATCCGCTTTTTTAAATAGCCCCACTGGCGGAAAA‑TGCTCATCATCACCCCAATAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                                          <  4:1008770/139‑1 (MQ=255)
                                                       aaaaaaTTTTCTGTGCGGTCTCAATCCGCGTTTTAAAATAGGCCCCGTGGCGGAAAT‑GGCCCACCACCACCACAAAAAACAACCACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGt                                                                                     <  1:1016148/139‑1 (MQ=255)
                                                             ttttCTGTGGCTTCTGAATCAGCGTTTTTAAATTGGACCACTGGGGGAAAA‑TGCTCATCAGCACCACAAAAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGa                                                                                 <  8:106487/138‑1 (MQ=255)
                                                             ttttCTGTGGCGTCTGATTCCGGGTTTTTAAATAGCCACAGTGGCGGAAAA‑TGCTCATACGAACCACTATATGTAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGa                                                                                 <  7:449817/138‑1 (MQ=255)
                                                                  tttgGCGAGTGAATCCGCGTTTTTAAATCTGACCACGGGGGGCAAA‑TGCTCATCAGCACCCCCATCACTAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGc                                                                          <  2:313506/138‑1 (MQ=255)
                                                                  tgtgGCTGCTGAAGCAGCTTTTTTAAATAGCACCACTGGCGCAAAA‑GGCTAACCCCCCCCACAAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGc                                                                          <  3:1129665/140‑1 (MQ=255)
                                                                    tgGCGTCTTAATACGCGTTTTAAAATCGGACCACTGGCGTCAAT‑TTCTCATCAGCACCACCATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTg                                                                        <  2:205472/140‑1 (MQ=255)
                                                                     ggCGTCTTAAGCAGCTTTTTAAAATAGGACCACTGGCGGAAAA‑TGATCTCCACCACCACCATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTgg                                                                       <  1:870932/140‑1 (MQ=255)
                                                                                 aGCTTTTTTAAATAGGACCAGTGGCGGCAAA‑TTCTAATCAGCACCCCAAAAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCa                                                           <  5:742978/140‑1 (MQ=255)
                                                                                 aGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCa                                                           >  6:895914/1‑140 (MQ=255)
                                                                                  gggTTTTTAAATAGCCCCCCTGGCGGCAAA‑TGCTCATCAGCACCACAATAACTAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGTGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaa                                                          <  6:953269/138‑1 (MQ=255)
                                                                                   cGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaa                                                         >  2:207268/1‑140 (MQ=255)
                                                                                       tttAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                                     >  5:921614/1‑140 (MQ=255)
                                                                                                gACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGAACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            >  2:378272/1‑140 (MQ=255)
                                                                                                gACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            >  5:180016/1‑140 (MQ=255)
                                                                                                gACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            >  1:957430/1‑140 (MQ=255)
                                                                                                gACCACTGGCGGCAAA‑TGCTCATCAACACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            >  6:372189/1‑140 (MQ=255)
                                                                                                ccccAGTGCCGGCAAT‑TGCTCACCCCACCCACCAAAAAAAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            <  2:113371/138‑1 (MQ=255)
                                                                                                    tcgggctgcAAA‑TGCTAATCACCACCTACATAAAGACACACGACCAGATCAAGCGATGCGTCATGATCTCATCTGCGGGCACGTAGTAAATCAGTTAGAAGTACGCTTGCGCAATACCCCAAATAAAATAAGCGGCaa                                          <  7:1036238/135‑1 (MQ=255)
                                                                                                    aCTGGCGGCAAA‑TGCTCATCAGCACCACCTTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCaa                                          <  8:627629/138‑1 (MQ=255)
                                                                                                     cctgcggcAAATTGCTCATCTGACCCAACTAAAAAAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAaga                                        <  3:1141464/137‑1 (MQ=255)
                                                                                                     cTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAagag                                       >  3:52928/1‑140 (MQ=255)
                                                                                                      tGGCGGCAAA‑TCGTCATCACCACAACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGGGCAATACCCCAAATAAAATAAGCGGCAaga                                        <  7:612882/138‑1 (MQ=255)
                                                                                                      gggcgtcAAA‑TGCTCATCAGAACCACAATAACGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAaga                                        <  7:788797/137‑1 (MQ=255)
                                                                                                      gggcggaAAT‑TGCTCATCAGCACCACAAAAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGc                                      <  3:616887/139‑1 (MQ=255)
                                                                                                      gggcgccAAA‑TGCTCATCACCCCCACCATAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGc                                      <  4:1091521/139‑1 (MQ=255)
                                                                                                        gcggcAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGa                                    >  6:189025/1‑140 (MQ=255)
                                                                                                          ggcAAA‑TGCTCAGCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGt                                  >  6:107472/1‑140 (MQ=255)
                                                                                                           gcAAA‑TGCTCATCATCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTa                                 >  2:880310/1‑140 (MQ=255)
                                                                                                           gcAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCCCGCGATGCGTCAGGATCTCATCGGAGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGAGGCAAGAGCGAGTa                                 >  4:738862/1‑140 (MQ=255)
                                                                                                             aaa‑TGCTCATCAGCACAACCATAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                               <  4:81294/140‑1 (MQ=255)
                                                                                                             aaa‑TGCTCAACCCCCCCCCCAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                               <  1:280890/140‑1 (MQ=255)
                                                                                                              aactGCGCATCACCCCCACAATAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATACGCGGCAAGAGCGAGTAAt                               <  2:686810/137‑1 (MQ=255)
                                                                                                                     catcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAAGACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGcc                          >  8:66494/1‑138 (MQ=255)
                                                                                                                      atcaGCACCACAATAAAGAAAAACGACCAGATCACGCGAAGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCtc                       >  1:751935/1‑139 (MQ=255)
                                                                                                                       accccaccacAATAACTAACCACGACCAGATCACGCGATGCGTCAGGATCTCATCGGAGGGCACGTAGTAAATCTGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGTCAAGAGCGAGTAATACGCCCt                        <  7:491188/134‑1 (MQ=255)
                                                                                                                           caccacCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCTGCAAGAGCGAGTAATCCGCCCTGCcgcg                  >  3:673908/1‑140 (MQ=255)
                                                                                                                            cccaccaTAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                 <  6:1097542/139‑1 (MQ=255)
                                                                                                                            accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                 <  6:448478/140‑1 (MQ=255)
                                                                                                                            accaccATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                 <  4:39604/140‑1 (MQ=255)
                                                                                                                            accaccATAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCAGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                 <  4:578378/140‑1 (MQ=255)
                                                                                                                             ccacaaAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                <  3:312381/140‑1 (MQ=255)
                                                                                                                                ccaTAAAGAAAAACGCCCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt             >  2:1059669/1‑140 (MQ=255)
                                                                                                                                ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt             >  3:333271/1‑140 (MQ=255)
                                                                                                                                ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCACTGCCGCgttt               >  8:378097/1‑138 (MQ=255)
                                                                                                                                 aaTAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgtt            <  6:115468/139‑1 (MQ=255)
                                                                                                                                   tAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGtttt          >  6:13584/1‑140 (MQ=255)
                                                                                                                                     aaGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGc        >  4:949586/1‑140 (MQ=255)
                                                                                                                                     aaGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTGTGAAGTACGCTGGAGCAATAACCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCATGCCGCGTTGGTTTTGc        >  2:754107/1‑140 (MQ=255)
                                                                                                                                      aGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca       <  2:556806/140‑1 (MQ=255)
                                                                                                                                       caaaaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGc        <  8:832106/137‑1 (MQ=255)
                                                                                                                                        aaaaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc     >  1:673364/1‑140 (MQ=255)
                                                                                                                                         aaaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcc    <  2:566808/140‑1 (MQ=255)
                                                                                                                                          aaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca   >  4:325516/1‑140 (MQ=255)
                                                                                                                                          aaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAACCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca   >  2:731065/1‑140 (MQ=255)
                                                                                                                                           aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat  >  1:108296/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAA‑TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT  >  NZ_CP022686/2769058‑2769335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A