Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F2 I0 R1	122	5985.5	7364544	98.3%	7239346	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,769,196	A→C	17.8%	F47C (TTT→TGT)	rarD ←	EamA family transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,769,196	0	A	C	17.8%	193.9 / 14.0	115	F47C (TTT→TGT)	rarD	EamA family transporter RarD
Reads supporting (aligned to +/- strand): ref base A (72/20); new base C (2/21); total (74/41)
Fisher's exact test for biased strand distribution p-value = 8.19e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT  >  NZ_CP022686/2769059‑2769335

cccAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGaaaacc                                                                                                                                           >  8:318094/1‑138 (MQ=255)

 ccAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTTAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGaaaaa                                                                                                                                            >  6:748269/1‑138 (MQ=255)

   aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCCGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  8:504716/1‑140 (MQ=255)

   aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  4:404014/1‑140 (MQ=255)

   aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  8:626579/1‑140 (MQ=255)

   aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  4:842975/1‑140 (MQ=255)

   aGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  8:443066/1‑140 (MQ=255)

     aTAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGCATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAACCGACCa                                                                                                                                      >  3:81975/1‑140 (MQ=255)

     aTAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAc                                                                                                                                        >  5:852769/1‑138 (MQ=255)

            gTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCa                                                                                                                                 >  6:126125/1‑138 (MQ=255)

               gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  7:629649/1‑140 (MQ=255)

               gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  4:929246/1‑140 (MQ=255)

               gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  3:473770/1‑140 (MQ=255)

               gCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGa                                                                                                                            >  3:704230/1‑140 (MQ=255)

                ccATTTGCCGCCAATCACCACGGAAGAGACTGCCAGAATAAAAATTTTCTGTGGCGTCTTAATCAGCGTTTTTAAATAGCCCCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAACAAACACGACCAGATCACGCGAt                                                                                                                           <  4:408591/140‑1 (MQ=255)

                    ttGCCGCCAATCAGCACGGCAGAGCCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCgg                                                                                                                       >  2:249937/1‑139 (MQ=255)

                    ttGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCCCTGGCGGCAAAT‑GCTCATCAGCACAAGCATAAAGCAACACGACCAGATCACGCGATGCgg                                                                                                                       >  3:817643/1‑139 (MQ=255)

                    ttGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCgg                                                                                                                       >  7:188876/1‑139 (MQ=255)

                     tGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTc                                                                                                                      >  4:540413/1‑140 (MQ=255)

                     tGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTc                                                                                                                      >  7:309367/1‑140 (MQ=255)

                     tGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTc                                                                                                                      >  8:209318/1‑140 (MQ=255)

                       ccgccAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTc                                                                                                                      >  5:468641/1‑138 (MQ=255)

                       ccgccAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAg                                                                                                                    >  1:690161/1‑140 (MQ=255)

                              tCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTGATCAGCACCACCATAAAGAAAAACTCCCATATCACGCGATGCGTCAGGTTCTCa                                                                                                             >  7:537601/1‑140 (MQ=255)

                              tCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCa                                                                                                             >  7:797389/1‑140 (MQ=255)

                              tCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGGTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCCTCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGagcgca                                                                                                             >  1:696968/1‑135 (MQ=255)

                              gcAGCACGGCCGACCCTCCCAGAAAAAAAATTTTCTGTGGCGGCTGAATCAGCGTTTTTAAAACGCACCCCTGGCGGAAAAT‑GCTCATCCCCACCACAAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCa                                                                                                             <  4:466538/139‑1 (MQ=255)

                                    cGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATcgg                                                                                                         >  5:295418/1‑138 (MQ=255)

                                        agagACTGCCTCAAAAAAATTTTTCTGTGGGGTATTTCTCAGTGTCTATAAATCCTCCCCCTGGCGGCAAAG‑CATCATCCCCACCCCCATAACCAAACTCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCggg                                                                                                     <  6:400794/138‑1 (MQ=255)

                                        agagACTGCCAGAATAAAAATTTTCTGTGGCGTCTCAATCAGCGTTTTAAAATAGCCCCACGGGCGGCAAAT‑GCTCACCCCCACCACAATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCa                                                                                                   <  2:604115/140‑1 (MQ=255)

                                         gagaCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTAAGGATCTCATCGGCGGGc                                                                                                    >  5:860398/1‑138 (MQ=255)

                                          cgccggCCAGCATAAAATTTTTCGGGGGCGGAGTAATC‑TCCTTTTTAATTACGCCCCCTGGGCGCAAATGGCTCATCTCCACAACCATAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCa                                                                                                   <  6:209823/133‑1 (MQ=255)

                                            aCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGCCCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACg                                                                                                 >  5:631133/1‑138 (MQ=255)

                                            aCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGAACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgta                                                                                               >  2:464452/1‑140 (MQ=255)

                                               gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGCCCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                            >  8:444923/1‑140 (MQ=255)

                                               gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCTGCTGGCACgtagga                                                                                            >  1:954210/1‑138 (MQ=255)

                                               gCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                            >  4:126555/1‑140 (MQ=255)

                                                ccAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGAACAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaa                                                                                           >  8:642610/1‑140 (MQ=255)

                                                 catgaTAAATATTTTCTGGGGAGGAGGAACGTGCGTTTTTACATAGGCCACCTGGCGCCATTT‑GGTCATCAGCCCAACCATAACGAAAAACGACCAGCTCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                                          <  8:526006/136‑1 (MQ=255)

                                                 cAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagtt                                                                                            >  6:148758/1‑137 (MQ=255)

                                                 aatcATAAAATTTTTCTGTGGTTTATTAATCCGCTTTTTAAAATTCAACCACTGGCGCAAAAT‑GATCATCAGCAACAAAAAAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACgtagta                                                                                            <  5:320782/135‑1 (MQ=255)

                                                 aagaaTAAAATTTTTTTGTGGCGTATTAATCAGGTTTTTTAAATCTGACCAGGGGCGGCAAAT‑GCTCATCACCCCCACAAAAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                                          <  3:304612/136‑1 (MQ=255)

                                                      aaaaatTTTTCTGGGGCGGCTGAATCCGCGTTTTTAAATAGGACCACTGGCGGCAATT‑GCTAATCAGCACCACAATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCa                                                                                       <  6:468641/137‑1 (MQ=255)

                                                       aaaaaTTTGCTGTGGCGTCTTAATCCTCGTTTTAAAATTGCACCACTGGCGTAAAAT‑TCTAATAACCACAACAATAAATAACAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGtt                                                                                    <  2:515966/140‑1 (MQ=255)

                                                                cTGTGGCGTCTGAATCAGCGTTTTTAAATAAGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTa                                                                             >  6:328377/1‑138 (MQ=255)

                                                                 tgtgcgttCTGACTCAGGTTTTTTAAAATTCACATCTGGCGGAATAT‑TCTCACCTGCACCACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGc                                                                          <  4:405492/133‑1 (MQ=255)

                                                                 tgggtggTCTGAAGCAGGTGTTTTAAATAGCACCATTGGCGGAAATT‑GCTAAACAGAACCACCATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGc                                                                          <  3:79780/134‑1 (MQ=255)

                                                                   gggCGTCTTACGCCGCTTTTATAAAATGCACCAGGGGCGTAAAAG‑GATCACCACCACCCCCATAACTAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTg                                                                        <  7:275060/139‑1 (MQ=255)

                                                                    ggcggcTTAATCAGCGTTTTTAAATAGTCCCACTGGCGGAAAAT‑GCTAATCAGAACCACCAAAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTgg                                                                       <  2:925001/140‑1 (MQ=255)

                                                                        tCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGgcg                                                                     >  6:736143/1‑138 (MQ=255)

                                                                         cTGAATCAGCGTTTTTAAATAGGCCCCCTGGGGGCAAAT‑GCTCATCAGCCCCACCATAAATAACCACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCaa                                                                  <  1:193378/140‑1 (MQ=255)

                                                                            aaTCAGCTTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATAc                                                               <  2:377274/140‑1 (MQ=255)

                                                                                agggTTTTTAAATCGGCCCCCGGGCGCCAAAT‑GCTCATCCCCCCCCCCAAAACTAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCa                                                           <  3:124063/137‑1 (MQ=255)

                                                                                      tttAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                                     >  1:223158/1‑140 (MQ=255)

                                                                                        tAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaa                                                     >  6:315883/1‑138 (MQ=255)

                                                                                         aatttgtaCCACTGGCGGAAATT‑GCTCATCAGCACAACAAAAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaata                                                  <  3:546761/133‑1 (MQ=255)

                                                                                         aaaTAGCCCCAGTGGCGGAAAAT‑GCTAATCCCCACAACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaata                                                  <  4:585916/140‑1 (MQ=255)

                                                                                         aaaTAGCACCAGTGGCGGAAAAG‑TCTCATCCGCACCACCATAAATAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaa                                                    <  5:698380/138‑1 (MQ=255)

                                                                                         aaaTAGCACCACTGGCGTAAAAT‑GCTAATCACCACCACAATAAACAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCaaataaaata                                                  <  1:547981/140‑1 (MQ=255)

                                                                                               taCCACTGGCGGAAAAT‑GCTCATCAGCACCACAATAAACAAAAACGACCAGATCTCGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCg                                              <  6:46718/137‑1 (MQ=255)

                                                                                               gACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            >  3:121104/1‑140 (MQ=255)

                                                                                               gACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            >  2:455686/1‑140 (MQ=255)

                                                                                               ccccccTGGCGGAATTT‑GATCATCAGCACCACCATAAAAAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGc                                            <  1:896928/135‑1 (MQ=255)

                                                                                                cccACTGGCGGAAAAT‑GCTCACCACCACCACAAAAAATAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCa                                           <  7:10475/139‑1 (MQ=255)

                                                                                                   aCTGGCGTCAAAT‑GCTAATAAGCACCACCATACAGAACAACGACCAGATCACGCGATGAGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCaa                                          <  6:771981/138‑1 (MQ=255)

                                                                                                     tggggtcAATT‑GCTCAGCCCCACCCCCATAAATAAACACGACCAGATCTCGCGTTGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAaga                                        <  6:299040/132‑1 (MQ=255)

                                                                                                     tGGCGGCAATT‑GATCATCATCACAGCCATAAAGAAAAACGAACAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACTTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCACAAATAAAATAAGCGGCAAGAGc                                      <  2:932738/140‑1 (MQ=255)

                                                                                                     tGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAaga                                        >  5:687298/1‑138 (MQ=255)

                                                                                                     gggcggcAATT‑GCTCACCAGCACCACCAAAACAAAACACGACAAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGc                                      <  2:516761/139‑1 (MQ=255)

                                                                                                      ggcgtcAAAT‑GCTCATCCGCACCAACATAAAGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCg                                     <  2:60874/140‑1 (MQ=255)

                                                                                                       gcggcAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCTGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTTGCGCAATACCCCAAATAAAATAAGCGGCAAGCGCGa                                    >  8:616599/1‑140 (MQ=255)

                                                                                                         ggcAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGt                                  >  3:637543/1‑140 (MQ=255)

                                                                                                          gcAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGCCCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTa                                 >  7:147678/1‑140 (MQ=255)

                                                                                                          gcAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTa                                 >  1:508351/1‑140 (MQ=255)

                                                                                                            aatt‑GCTCATCCGCACCACAATAACGAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                               <  7:504716/137‑1 (MQ=255)

                                                                                                            aaaT‑GCTCACCAGCACCACCAAAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                               <  7:626579/140‑1 (MQ=255)

                                                                                                            aaaT‑GCTAATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGAAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTa                                 >  5:256407/1‑138 (MQ=255)

                                                                                                              aT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                               >  6:898384/1‑138 (MQ=255)

                                                                                                                    catcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                        >  1:256806/1‑140 (MQ=255)

                                                                                                                      acaccaccacCAAAAATAAAAACGACCAGAGCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGc                      <  4:822988/136‑1 (MQ=255)

                                                                                                                           cccaccaTAAACAAACACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                 <  2:749124/139‑1 (MQ=255)

                                                                                                                           acccccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                 <  7:618111/140‑1 (MQ=255)

                                                                                                                            ccaccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                >  2:34120/1‑140 (MQ=255)

                                                                                                                               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt             >  2:187762/1‑140 (MQ=255)

                                                                                                                               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt             >  3:120493/1‑140 (MQ=255)

                                                                                                                                 aTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCATTACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt             >  5:443265/1‑138 (MQ=255)

                                                                                                                                     ataaacaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca       <  2:671448/138‑1 (MQ=255)

                                                                                                                                     acaaacaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca       <  7:432912/138‑1 (MQ=255)

                                                                                                                                     aaaaacaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca       <  7:464776/138‑1 (MQ=255)

                                                                                                                                       aaaaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc     >  2:683818/1‑140 (MQ=255)

                                                                                                                                         aaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTaaa                                                                                          >  7:784178/1‑53 (MQ=255)

                                                                                                                                         aaaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca   >  7:809546/1‑140 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCTATGCGTCAGGATCTCATCGGCGTGCACGTAGTAAATAAGTTTGAAGTAAGCTGGCGCAATACCCCAAATAAAATAAGAGGCAAGAGCGAGTATTACGCCCTGCCGCTTTTGTTTTGcatccat  >  8:266764/1‑140 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACg                                                                                                 <  4:104156/45‑1 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTTGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca       <  8:549164/135‑1 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat  <  2:898431/140‑1 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat  >  3:381814/1‑140 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat  <  4:642059/140‑1 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat  >  1:458123/1‑140 (MQ=255)

                                                                                                                                          aaCGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca       >  7:549164/1‑135 (MQ=255)

CCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAAT‑GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATCTCATCGGCGGGCACGTAGTAAATCAGTTTGAAGTACGCTGGCGCAATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT  >  NZ_CP022686/2769059‑2769335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A