Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,546,996 T→A 15.8% intergenic (+18/‑16) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,546,9960TA15.8% 142.8 / 2.9 86intergenic (+18/‑16)CIW80_RS07705/CIW80_RS07710tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (9/61);  new base A (11/5);  total (20/66)
Fisher's exact test for biased strand distribution p-value = 1.65e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTTGCATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGG  >  NZ_CP022686/1546858‑1547131
                                                                                                                                           |                                                                                                                                        
tggTTGCATGAAAACGCGAGCATTGTATAGTGCGCATCCACGGTCTCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCTGTTCAAATCCGGCCCCCGGAACAAATTAAAATTTGAT‑GAAGTa                                                                                                                                         <  3:924632/139‑1 (MQ=18)
aGGTTGCATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGTCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCAGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTa                                                                                                                                         <  3:799774/140‑1 (MQ=255)
aGGTTGCATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTa                                                                                                                                         <  6:259366/140‑1 (MQ=255)
   ttGCATGATAAGGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCTTAACCCGAAGGTCGTCGGTTCAAATCCGTCACCCGCAACCAATTAAAATTTGAT‑GAAGTAAAg                                                                                                                                      <  5:371281/140‑1 (MQ=18)
   ttGCATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAg                                                                                                                                      <  3:77906/140‑1 (MQ=255)
   ttGCATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAg                                                                                                                                      <  2:404941/140‑1 (MQ=255)
   ttGAATGAATACGCGAGCAGATTATAGTGGTCATGCACTGACGCGGGGTTTAGCATCCTGGTAGCTAGTCGGGCTCATAACCCGATGGTCGTCGGTTCAAATCCGGACCCCGCAACCAATTAAAATTTGTT‑GAAGTAAAg                                                                                                                                      <  2:404942/140‑1 (MQ=17)
   gtGCATGAAAACGCGAGCAGAGTATAGTGCGAATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCGCGTCGGGCTCATAACCCGAAGGTCGTCGGTTAAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAg                                                                                                                                      <  4:247923/139‑1 (MQ=255)
      cATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAATTGAT‑GAGGAAAAGCAg                                                                                                                                   >  3:604486/1‑140 (MQ=255)
        tGAAAACGCGAACAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGTTAGCGCGTACGGCTACTAACCCCAACGGCGTCGGTTCAAATCCGGCAACAGCAACAAAAAAATATTTGAT‑GAAAAAAAGCAg                                                                                                                                   >  7:685562/1‑138 (MQ=12)
           aaaCGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTAc                                                                                                                                <  7:823865/138‑1 (MQ=255)
                cgAGCAGAGTATAGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGAcg                                                                                                                         <  4:837285/140‑1 (MQ=255)
                cgAGCAGAGTATAGTGCGCATCCACGGACGCGGTGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGa                                                                                                                           <  8:309410/138‑1 (MQ=255)
                cgAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCATTTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGAcg                                                                                                                         <  5:490466/140‑1 (MQ=255)
                cgAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGa                                                                                                                           <  8:43468/138‑1 (MQ=255)
                cgAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGAcg                                                                                                                         <  4:553821/140‑1 (MQ=255)
                cgAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCACGCAACCAATTAAAATTTGAT‑GATGTCTAGCAGTACGGGGAcg                                                                                                                         <  6:649726/140‑1 (MQ=18)
                     cgagCATAGTTCGCCTCCCCGGACGCGGGGAGGAGCAGCCTGGTAGCTCGTCGGGCGCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATATGAT‑GAAGTAAAGCAGTACGGTGCCGCg‑ggg                                                                                                                   <  6:206573/139‑1 (MQ=14)
                     agagTATCGTGCGCAGCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAACTAAAGCAGTACGGTGACGCg‑ggg                                                                                                                   <  6:271243/140‑1 (MQ=255)
                     agagTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCg‑ggg                                                                                                                   <  1:574282/140‑1 (MQ=255)
                        gTATAGGGCGCATCCACGGACGCGGGGGGGAGCAGCCTGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTgg                                                                                                                <  3:265783/140‑1 (MQ=255)
                          ataggccgcAGCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCACGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGag                                                                                                              <  4:523098/134‑1 (MQ=14)
                          ataGTGCGCATCCACGGACGCGGGGTGGATCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAATGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGag                                                                                                              <  5:161742/140‑1 (MQ=255)
                          ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTgg                                                                                                                <  8:333602/138‑1 (MQ=255)
                          ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGag                                                                                                              <  1:950657/140‑1 (MQ=255)
                          acaGTGCGCATCCACGGACGCGGGGGGGAGCAGCCGGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACAAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGag                                                                                                              <  4:714965/138‑1 (MQ=17)
                           taGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATT‑AAATTTAATGGTAAAAAAGCAGTAAGGTGACGAG‑GGGTTGa                                                                                                               >  7:464121/1‑138 (MQ=14)
                                cgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCAGGCCCCCGCTCCCAATTAAAATTTGAT‑GTAGTAAAGCAGTACGGGGACGCG‑GGGTGGAGCAGAAt                                                                                                        >  3:169284/1‑140 (MQ=14)
                                 gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATAATTGAG‑GATTAAAAGCCGTACGGTG‑CGTGTGGGTGGagcaggttt                                                                                                       >  5:287706/1‑136 (MQ=14)
                                 gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATAATTGAG‑GAAAAAAACCAGTACTGTGCCGGT‑GGGTGGagctgcttt                                                                                                       >  3:221939/1‑137 (MQ=14)
                                 gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATATTTGAT‑GAAGAAAAGCAGAACGCTGCCGCG‑GGGTGgcgctggatt                                                                                                       >  1:303025/1‑134 (MQ=14)
                                   ttCCACAGACGCGGGGTGGAGCAGCCTGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCAGGCCCCCGCAACCAATTAAACTTTGAT‑GAAGAAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGt                                                                                                     <  3:879058/139‑1 (MQ=14)
                                   aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTAATTTAGTT‑CATCAAAACCGGTAAGGTGCCGCG‑GGGTGGAGGAGCATTGt                                                                                                     >  3:767720/1‑140 (MQ=14)
                                   aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATTATAGAT‑GTAGAAAAGCTCTACGGTGCCGAG‑GGGTGGAGGAGGAtt                                                                                                       >  8:405371/1‑137 (MQ=14)
                                   aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATATGTTAT‑GAAAAAAAGCACTACGGTGCATCG‑GGGTGGAGCAGCATggg                                                                                                     >  5:254849/1‑139 (MQ=14)
                                   aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTa                                                                                                                                         <  3:799597/105‑1 (MQ=255)
                                   aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTa                                                                                                                                         >  4:799597/1‑105 (MQ=255)
                                     ccACGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATATTATAT‑GAGAAAAACCAGTACGCTGACTCT‑GGGTGGagcagattggg                                                                                                     >  8:480812/1‑132 (MQ=11)
                                          gACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTAAAATTGAT‑GTTTAAAAGCCGTACGGTGACGCG‑GGGTGGAGCAGATTGGTAGCtc                                                                                                >  7:343412/1‑138 (MQ=11)
                                          caCGCGGGGTGGAGCAGCCTTGTAGCGCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGCCCCCCGCAACCAATTAAAATTTGCA‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCtcgt                                                                                              <  1:361132/139‑1 (MQ=14)
                                               ggggggGAGCAGCCTGGTTTCTCGGACGGCTCATAACCCGTACGGGGTCGGTTCAAAACCCGCCCCCGCAACCAAATAAAATTTGAA‑GCACTAAACCACTACGGTGACGCG‑GGGGGGAGCAGCATGGTAGCTCGTCGGGc                                                                                         <  2:714762/140‑1 (MQ=255)
                                                  ggggAGCAGCCTGTTAGTGAGGCGGGCTCATAACCCGAAGGGGGTCGCTGCTTCTCAGGCCCCCGCAACCAATTACAAATTGCT‑GAAGAATTGCAGTCCGGTGACGCG‑GGGGGGAGCTGCAGGGTAGCTCGTCGGGCTCa                                                                                      <  2:708517/138‑1 (MQ=9)
                                                  ggggAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCa                                                                                      <  1:4799/138‑1 (MQ=255)
                                                   tGGAGCAGCCTGGTAGCTCGTCGGGATCATAACCCGAAGGGCGTCGGTGCAAATCCGGCCCCCGCAACCAATTAAAATTAGAT‑GAATTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTc                                                                                       <  7:566118/138‑1 (MQ=11)
                                                   gggAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAACCGGCCCCCGCAACCAATAAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCAt                                                                                     <  5:747377/139‑1 (MQ=255)
                                                   gggAGCAGACTGGTAGCTCGGCGGGCTCAGAAGCCGCATGTCGTCGGTGCAAAACCGGCCCCCGCAACCAATAAAAATTTGAA‑GTAGTCTAGCAGTCCGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCAt                                                                                     <  4:249316/139‑1 (MQ=11)
                                                       gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGagcag                                                                                                           <  4:358703/114‑1 (MQ=39)
                                                       gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGagcag                                                                                                           >  3:358703/1‑114 (MQ=39)
                                                         agcGTGGTAGCTCGTCGGGCACTTAACCCGAATGTCGTCTGTTCAAATCCGGCCCCAGCAACCAATTAAAATTTTAT‑GAAGTTAAGCAGTCCGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAAcc                                                                                 <  7:227462/138‑1 (MQ=11)
                                                         agcCTGGTTGCTCGGCGGGCTCCTAACCCGACGCTCGGCTGTTCAAATCCTGCCCCCTCAAACTATTAAAATTTGAT‑GAAGTCACTCAGTCCGGTGACGCG‑GGGTGGAGCAGCATGGTAGCGCGTCGGGCTCATAACCCg                                                                               <  1:767108/140‑1 (MQ=14)
                                                                 tgCTCGGCGGGCTCTTAACCCGAAGGTCGTCGGTTCTAATCCGGACCGCGCAACCAATTATAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                       <  1:329200/139‑1 (MQ=11)
                                                                     cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTa                                                                                                                                         <  4:717693/71‑1 (MQ=39)
                                                                     cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTa                                                                                                                                         >  3:717693/1‑71 (MQ=39)
                                                                     cgtcgGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCCCCCAATTAAAATTTGAT‑GAAGTAAAGCAGTTCGGTGTCGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgggggg                                                                   >  3:48272/1‑135 (MQ=11)
                                                                              aaTAACCCGAAGGGCGGCGGGTCAAATCCGGCCCCCGCAACCAATTAAAAATTGAT‑GAAGTCAAGCAGTCCGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                                                          <  6:251466/139‑1 (MQ=11)
                                                                               atatCCCGAAGGCCGTCGGTTCTAATCCCGCCCGCGCAACCAATTAAAATTTGCT‑GAAGTAAAGCATTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                         <  1:733053/140‑1 (MQ=11)
                                                                                       aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGAcg                                                                                                                         <  1:751880/69‑1 (MQ=40)
                                                                                       aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGAcg                                                                                                                         >  2:751880/1‑69 (MQ=40)
                                                                                                  gtCAAATCCGGCCCCCTCAACCAAATAAATTTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaaa                                      <  2:962909/139‑1 (MQ=255)
                                                                                                       atccttcccccTCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtga                                 <  2:470035/134‑1 (MQ=255)
                                                                                                       aacctgCCCCCGCAACCAATTATAATTTTTA‑GAAGTTAAGCAGTACGGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtga                                 <  5:815262/135‑1 (MQ=14)
                                                                                                       aaacgccccccGCAACCAATTAAAATTTGAT‑GAAGAAAACCCGTACGGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtga                                 <  6:284751/134‑1 (MQ=14)
                                                                                                       aTCCTGCCCCAGCAAGCAAATAAAATTTGAT‑GAAGTTAAGCAGTAAGGTGACGCG‑GGGTGTAGGAGCATGGTATCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGACCCCGCAACCAATCAAAAttt                                   <  8:287823/138‑1 (MQ=14)
                                                                                                       aTCCTGCACGCGCAACCAATAAAAAATTGAA‑GAAGAAAACCACTACTGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtga                                 <  2:854038/140‑1 (MQ=14)
                                                                                                       aTCCGGCCCCCGCAAACAATTAAAAATTGCT‑GAAGTAAAGCAGTCCGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtga                                 <  1:215494/140‑1 (MQ=255)
                                                                                                       aTCCCGCCCCCGCAACCAATAAAACTTTGAT‑GAAGTAACGCACTCCGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtga                                 <  3:336726/140‑1 (MQ=14)
                                                                                                         ccctcccccGCAAACAATTAAATATTTAT‑GAATTATAGGATTACTCTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTtgatg                               <  2:511979/136‑1 (MQ=14)
                                                                                                            gCCCCCGCAACCAATTAAAATTTTAT‑GAATTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTAGTCGGGCTCATACCCCGAAGGTCGTCGGTTCACATACGGCCAACGCAACCAAAGAAAAATtgatgaca                            >  1:561502/1‑138 (MQ=14)
                                                                                                            cgccccGCAACCAAATAAAATTTGAT‑GAAGTAACGCAGTCCGGTGACGCG‑GGGTGGAGCAGCATGGGAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGaaa                            <  4:885972/138‑1 (MQ=17)
                                                                                                              cgccGCAATCAATTAAATTTTGAA‑GAATTATCCCATTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATa                          <  2:207969/138‑1 (MQ=17)
                                                                                                              ccccTCAAACAAATAAAATTAGAT‑GAATTAAAGCAGTACTGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATa                          <  4:615258/140‑1 (MQ=17)
                                                                                                              ccccGGAACCAATTAAATTTTGAT‑GAAGTATAGCCGTACGCGGACGCG‑GGGTGGAGCAGCAAGGTATCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATa                          <  1:914331/140‑1 (MQ=17)
                                                                                                              ccccGCCACCAATTCAAATTTCAA‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGaaa                            <  7:521538/138‑1 (MQ=255)
                                                                                                              ccccGCAAGCAATTAAATTTTGCT‑GTATTTAAGCAGTACGGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATa                          <  1:833413/140‑1 (MQ=17)
                                                                                                              ccccGCAACCAATTAACATTTGCT‑GAAGTCAAGGCCTCCGGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATa                          <  4:275654/140‑1 (MQ=17)
                                                                                                                 cGCAACGAATTAAAATTTGAT‑GAAGTAAAGCATTACGGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                       <  2:655986/140‑1 (MQ=255)
                                                                                                                     aacAATTAAAATTTGAT‑GAAGTAAAGCAGTCCGGGGACGCG‑GGGTGGATCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCa                     <  7:621938/136‑1 (MQ=255)
                                                                                                                              agtatcat‑gaAGTAACGGAGTCCGGGGACGCG‑GGGTGGAGCAGCATGGTAGCTCGGCGGGCTCATAACCAGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGa            <  8:39835/132‑1 (MQ=14)
                                                                                                                              acttagct‑gaAGTAAAGCAGTTCGCTGTCGAG‑GGGCGGAGCAGCCTGGTAGCTCGTCGTGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCCGTACGGTGAcg          <  6:649037/133‑1 (MQ=14)
                                                                                                                              aaattgtt‑gAATAAAACCATTACGCTGACGCG‑GGGTGGAGCAGCATGTTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg          <  6:286010/137‑1 (MQ=14)
                                                                                                                              aaattGCT‑GACGTAACCCACTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg          <  5:425209/137‑1 (MQ=14)
                                                                                                                              aaTTTTAT‑GAATTTAAGCAGTACCGCGACGCG‑GGGTGGATCAGCATGGGAGCTCGGCGGGCTCATAACCCGAAGGTCGGCGGGTCCAATCCGGCCCCCGCAACCAATCAAAATTTGAAGAAATAAAGCAGTACGGTGAcg          <  4:64045/140‑1 (MQ=14)
                                                                                                                              aaTTTTAT‑GAAGTAACGCATTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCTGCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg          <  6:503858/140‑1 (MQ=17)
                                                                                                                                  tgat‑gaAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCtcgtcg                                                                                            >  2:592396/1‑54 (MQ=255)
                                                                                                                                  tgat‑gaAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCtcgtcg                                                                                            <  1:592396/54‑1 (MQ=255)
                                                                                                                                    at‑gaAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATATTAATGAAACAAAGCAGAACGCTGCAGCGGGGt    >  5:765127/1‑140 (MQ=14)
                                                                                                                                         actAAACCAGTACGGTGCCGCG‑GGGTGGAGCATCTTTGTAGCGCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg  <  8:697137/136‑1 (MQ=14)
                                                                                                                                           |                                                                                                                                        
AGGTTGCATGAAAACGCGAGCAGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑GAAGTAAAGCAGTACGGTGACGCG‑GGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGG  >  NZ_CP022686/1546858‑1547131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A