Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,633,868 C→A 24.7% E246* (GAA→TAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,633,8680CA24.7% 262.8 / 16.5 148E246* (GAA→TAA) yqeBselenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand):  ref base C (24/76);  new base A (41/2);  total (70/78)
Fisher's exact test for biased strand distribution p-value = 1.77e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTGAACCTTGCGGCGA  >  NZ_CP022686/1633732‑1634007
                                                                                                                                         |                                                                                                                                            
cccGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCt                                                                                                                                             <  8:567711/138‑1 (MQ=255)
cccGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGc                                                                                                                                           <  4:891741/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                                                        <  1:136192/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                                                        <  6:743995/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                                                        <  3:111709/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                                                        <  2:540071/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                                                        <  4:70258/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                                                        <  2:696781/140‑1 (MQ=255)
   gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTAACCAG‑TAATATTTCTGCCAg                                                                                                                                        >  1:447739/1‑140 (MQ=255)
     aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAAACGG‑TAATATTTCTGcccc                                                                                                                                        >  8:306112/1‑136 (MQ=255)
         tACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCAACTCGAATCAACACCAGCTGGTGTCAGGAAGGATGAGAAGGCTTCATCATAAACCCACCCGTCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCt                                                                                                                                  <  5:698615/140‑1 (MQ=255)
         tACAGACGTAGAGCTACACCACTGGCGAAATCCCCCTCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCt                                                                                                                                  <  5:698612/140‑1 (MQ=255)
           cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTaa                                                                                                                                <  1:927514/140‑1 (MQ=255)
           cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTaa                                                                                                                                <  2:496317/140‑1 (MQ=255)
              aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTc                                                                                                                                              <  1:784105/123‑1 (MQ=255)
              aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTc                                                                                                                                              >  2:784105/1‑123 (MQ=255)
              aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCAG‑GAAAATTTATTCCAGAACGCTAAAgg                                                                                                                             >  3:819407/1‑140 (MQ=255)
              aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCTG‑TAATATTTCTGCCAGCCCGCTaaatg                                                                                                                             >  2:407659/1‑137 (MQ=255)
              aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCGGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATATTTCACCTG‑TTATATTTATGCCAGCCCGCGAATgg                                                                                                                             >  4:892588/1‑140 (MQ=255)
                ttAGCGCTACACAACTGGCGCTATCCGACGCACCGCGAATCACCTCCAGCTTGTTTGAGGAAGGATGAGAATGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCTCCTG‑TTATATTTCTTCCAGCACTATAATGGCg                                                                                                                           <  6:195765/139‑1 (MQ=255)
                gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCg                                                                                                                           <  3:635363/140‑1 (MQ=255)
                 tAGCGCTACACCACTGTCGATATCCCCCGCCCCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTACCCGG‑TAATATTTATGCCAGCCCCCTAAAGGCgg                                                                                                                          >  2:234571/1‑139 (MQ=255)
                 tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATAAACCCACCCGGCGCGGTATTTTTAAACTG‑TAATATTTATTCCAGCACGCGAAttggga                                                                                                                          >  5:319542/1‑135 (MQ=255)
                  aGCGATACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTAATTTTAACCTG‑TAATATTTATTCCAGCACGCTAAATGCGaa                                                                                                                         >  3:722935/1‑139 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATTAGAAGGCTTCATCATCAACCCACCCGGCCCGGTATTTTTAAACCG‑GAATAATTCTTCAACCACGATAATGGCAAtc                                                                                                                        >  1:446062/1‑140 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCAGGCGCGTTTATTTTAACCTG‑TAATATTTATGCCCGACCGCTaagtgcgg                                                                                                                          >  8:569461/1‑132 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCTG‑TAATATTTATTCCAGCACGCTAATGGCGCtc                                                                                                                        >  6:726187/1‑140 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTAACAG‑TAAAAATTTTGACAGCAACCGAATGGGGAta                                                                                                                        >  1:631976/1‑139 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTACAGG‑TTATATTTATTCACGCACCCTAATgtggata                                                                                                                        >  6:881011/1‑134 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGGGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACAAG‑TAATATTTATGCCAGCACGCTAAGGGCGAtc                                                                                                                        >  6:551252/1‑140 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGCTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCAG‑TTATATTTCTTCAAACACCCGAATGACGaac                                                                                                                        >  2:945779/1‑138 (MQ=255)
                   gcgcTACACCACTGGCGATATCCCCCGCACAGCGAATCACCACCAGCTGGTGTCAGGAAGGATGAGAAGGCTTCATCATCAACCAACCCGGCGCGTTATTTTTAACCTG‑TAATATTTATTACACCACGCTAAAGGcagaa                                                                                                                        >  6:146329/1‑136 (MQ=255)
                     gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCCCCCGGCGCGGTGTTTTTACGCGG‑TAATATTTCTGGCAGCACGCGAAGGGCggtg                                                                                                                        >  7:115159/1‑135 (MQ=255)
                     gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCAG‑TAATATTTATGCCAGCACGCTAAAGGCGAta                                                                                                                        >  8:459349/1‑137 (MQ=255)
                     gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAAGCGG‑TAATAATTCTTCCACCACGCTAAAGGCGAtc                                                                                                                        >  7:219940/1‑138 (MQ=255)
                        acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAACCTG‑TAATATTTATGCCCGAACACTAATGGCGAtatacag                                                                                                                   >  2:862486/1‑134 (MQ=255)
                        acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTAACCAG‑TAATATTTGTTCCCGCCCCCTAATGGCGCTCTCCTg                                                                                                                   >  2:426017/1‑140 (MQ=255)
                           ccacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTgcgg                                                                                                                <  4:344839/140‑1 (MQ=255)
                              cTGGCGATATCCCCCGCACCGCGCATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCTCCCGGCGCGGGTTTTTTGACCTG‑TAATATTTAATCCGGCACGCCGATTGCGCACTCCTacggcgg                                                                                                             >  2:149747/1‑139 (MQ=255)
                              cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGCTGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCGG‑TAATATTTATGGCCGCACGCGACTGGCGCTCTCCTccggcgg                                                                                                             >  1:785686/1‑139 (MQ=255)
                              cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCAACACGGCGCGTGATTTTTAACCTG‑GAATATTTGTGGCAGCACGCTAATGGCGATCTCCAGCGGCGt                                                                                                             >  6:871515/1‑140 (MQ=255)
                              cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAGACTG‑TTATATTTATGCCAGCACCCGTATGGCGATCTCCGtcggcgg                                                                                                             >  5:824510/1‑139 (MQ=255)
                              cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGTTTTTTCACCTG‑GATTATTTCTGCCAGCCCACTAATGGCGATCTCCTgcggcgg                                                                                                             >  5:778512/1‑139 (MQ=255)
                              cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCTGTTAATA‑TTCTTCCAGCACCCTAAATCAGATCTACTgcggcct                                                                                                             >  4:107419/1‑138 (MQ=255)
                                 gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                                                          <  1:260649/140‑1 (MQ=255)
                                 gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                                                          <  4:147131/140‑1 (MQ=255)
                                 gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTGCCTG‑GAAAATTTATGGCCCCACGCATATTGCGATCAACAGCCGCGtttg                                                                                                          >  1:91069/1‑139 (MQ=255)
                                 gCGATATCCACCGCACAGCGAATCACCACCAGCTGGTGTCCGGAAGTATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                                                          <  4:487720/140‑1 (MQ=255)
                                   gagatCCCCCGCACCGCGAATCACCACCAGCGGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCTCCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                                                        <  1:598508/137‑1 (MQ=255)
                                   gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                                                          <  7:79349/138‑1 (MQ=255)
                                   gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                                                        <  6:289054/140‑1 (MQ=255)
                                   gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                                                        <  3:701623/140‑1 (MQ=255)
                                   gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACAAG‑TAAAATATATGCCAGCACACTAATGGCGCTAAACTGCGGCGttta                                                                                                          >  8:394978/1‑137 (MQ=255)
                                   gATATCCCCCGCAACGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCTTCTCTTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCTGCGTTTCCg                                                                                                        <  6:703097/140‑1 (MQ=255)
                                        cccccGCACCGCGATTCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                                                   <  5:451739/140‑1 (MQ=255)
                                        cccccGCACCGCGAATCACCACCAGCTTGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                                                   <  3:643057/140‑1 (MQ=255)
                                        cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGTAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                                                     <  7:829813/138‑1 (MQ=255)
                                        cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                                                   <  2:499001/140‑1 (MQ=255)
                                           ccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACCAG‑TTATATTTCTGCCACCACGCGAATGGGGAGCTCCAGGGGCGGTTGCGCACCAAtt                                                                                                >  1:863195/1‑139 (MQ=255)
                                            cGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTTTTTTTTACCGG‑GAATTATTGAGGGCGCCCGCGAATGGCGGTCTCCGGCGGCGGTTTCGCACCTAtgt                                                                                               >  5:222201/1‑140 (MQ=255)
                                             gCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCTG‑TAAAATTTAAGCCAGCACGCGAATGGCGATCTCCAGCGGCGTTTGCGCACCAAtt                                                                                                >  8:410720/1‑137 (MQ=255)
                                               aCCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTACAGG‑TTAAATTTATGCCAGAACCCTAAAGGGGGGATCCAGCGGCGTGTAAGCACCAAtgtttt                                                                                            >  5:557230/1‑138 (MQ=255)
                                                   cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTTTTTTTAGACAG‑TAATATTTGTGGCAGCACGCTAATTGGGATCTACTGGGGCGTTTCCGCACCTATGTTG‑TAAcc                                                                                       >  4:568267/1‑140 (MQ=255)
                                                   cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCTG‑GAATATTTATGCCAGCACGCTAATTGCGATCACCTGCGGCGTTTCCGCACCGATGTTG‑TAAcc                                                                                       >  4:501063/1‑140 (MQ=255)
                                                         accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACg                                                                                   <  8:151064/138‑1 (MQ=255)
                                                          ccaccaGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGGTATTTTTAACCGG‑AAATATATATGCCCGCACACTAATAGGGATCGCCTTCGGCGTTTACCCAACAAAGTTGTTAACAAACg                                                                                   >  8:309072/1‑138 (MQ=255)
                                                          ccaccaGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGgc                                                                                <  5:31915/140‑1 (MQ=255)
                                                           caccaGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCTG‑TTATATTTATTCCCGCACCCGAATTGCGATCTCCTGCGGCGTTTACGCCCCTATTTTT‑TTAACAACGGgcg                                                                               >  2:397191/1‑140 (MQ=255)
                                                             ccaGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCTG‑TAATATTTCTTCCCGCACCGGAATGGCGATCTCCTGCGGCGGTTCCGCACCAATGTTG‑TAAACAACGGgcc                                                                               >  8:10574/1‑137 (MQ=255)
                                                                gCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAAACTG‑TAATATTTCAGCCAGCACGCTACATGCGATCTACAGCGGCGGTTCCGCCCCTATGTTG‑TAACCAACGGCCGCATGt                                                                          >  2:108524/1‑140 (MQ=255)
                                                                   tgtgtCCGGCAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTa                                                                         <  8:428492/137‑1 (MQ=255)
                                                                   ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTaaa                                                                       <  2:326711/140‑1 (MQ=255)
                                                                   ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACACACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTaaa                                                                       <  6:86092/140‑1 (MQ=255)
                                                                    gtgtCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAAc                                                                      <  5:227113/140‑1 (MQ=255)
                                                                      gtCCGGAAGGATGAGAAGGCTTCCTCATCAACCCCCCCGGCTCGGTATTTTTCACCTG‑TAATATATCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTCTGTAG‑TAACCAACGGGCGCATGTAAACgg                                                                    <  2:754764/140‑1 (MQ=255)
                                                                       tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGGTTCCGCACCTATTTTG‑TAACCAACGGGCGCATGTAAACggg                                                                   <  3:251395/140‑1 (MQ=255)
                                                                         cGGAAGGATGAGCAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCa                                                                 <  3:614983/140‑1 (MQ=255)
                                                                                 tGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACATG‑TAATATTTATTCCAGCACGCTAATGGCGATCTACAGCGGCGTTTCCGCCACTATTTTG‑TAACAAACGGGCGCAAGTAAAAGGGCAAAATGtta                                                         >  2:699876/1‑139 (MQ=255)
                                                                                       ggCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCTCGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCa                                                     <  7:560222/138‑1 (MQ=255)
                                                                                       ggCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACTGGCGCATGTAAACGGGCAATATGTTCTTCa                                                     <  7:785150/138‑1 (MQ=255)
                                                                                       ggCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGc                                                   <  4:313952/140‑1 (MQ=255)
                                                                                        gCTTCATCATCAACCCACCCGGCGCGGTATTTTTGCCCTG‑TAATATTTATGCCAGCACGCTAATGGCGATCTCCTGCGGCGTGTCCCCCCCGATGTTG‑TAACCAAAGGGCGCATGTAAACGGGCCATATGTTCTTCAGcc                                                  >  2:934558/1‑140 (MQ=255)
                                                                                         cTTCATCATCAACCCACCCGGAGCGGTAATTTTAAACGT‑GAATATTTATGCCAGCCCCCTAATGGCGAAAAAATGCGGCGATTACGCACCGAATTTG‑TAACCAACGGGCGCATGTAAACCGGGAATATGTTATTTATCCa                                                 >  2:225890/1‑140 (MQ=255)
                                                                                          ttCATCATCAACCCACCCGGCGCGGTTTTTTTCACCTG‑TAATATTTATTGCAGCACGCGAAAGGCGATCTCCTGCGGCGGTTCCGCCCCCATGTTG‑TTACCAACGGGCGCATTTTAACGGGCAATATTTTCTTCATCCAc                                                >  6:220753/1‑140 (MQ=255)
                                                                                            catcatcaACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCAc                                                <  7:562694/138‑1 (MQ=255)
                                                                                              tcatcaACCCACCCGGCGCGGTATTTTTAACCAG‑TAATATTTATGCCCGCCCGCTAATTGCGCTCTCCTGCGGCGTTTACGCCCCAATGTTT‑TTACCCACCGGCGCATGTAAACGGGGAATATTTTGTTGAGCCCCGc                                              >  7:856670/1‑138 (MQ=255)
                                                                                                    acccacccGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCa                                      <  6:472016/140‑1 (MQ=255)
                                                                                                           cGGCGCGGTATTTTTGACCTG‑TAATATTTCTGCCCGCACGCGAATTGCGATCTCCTGCGGCGTTTACGCCCCGATTTTG‑TAACCCACGGGCGCATGTAAACGGGCAATATTTTCTTCAGCCACGCCTTTCTCACGCaa                                 >  8:820961/1‑138 (MQ=255)
                                                                                                              cgcgTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGgc                            <  6:346770/140‑1 (MQ=255)
                                                                                                              cgcgTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGgc                            <  3:75497/140‑1 (MQ=255)
                                                                                                                   tATTTTTCACCTG‑TAATATTTCTTCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAgg                       <  1:867087/140‑1 (MQ=255)
                                                                                                                     tttttCACCTG‑TAATATTTCTGCCATCACGCTAATGGCGATCTCCTGCGGCGCTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGaa                     <  6:372255/140‑1 (MQ=255)
                                                                                                                     tttttCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGaa                     <  6:419007/140‑1 (MQ=255)
                                                                                                                             cTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTGaa             >  6:723927/1‑140 (MQ=255)
                                                                                                                              tG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCAGGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTGAAc            <  5:759375/140‑1 (MQ=255)
                                                                                                                                        tCTGCCAGCACGCTAATGTCGATCTACTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTACACGGGCAATATGTTATTCAGCCACGCCGTTCTCCCGCAAGTGGCGCAGGAAAAGCTGAACCTTgcggcg   >  5:801112/1‑140 (MQ=255)
                                                                                                                                        tCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATg                                                            >  3:482289/1‑83 (MQ=255)
                                                                                                                                        tCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATg                                                            <  4:482289/83‑1 (MQ=255)
                                                                                                                                        tCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTGAACCTTgcggcg   >  2:513016/1‑140 (MQ=255)
                                                                                                                                         cTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTGAACCTTGCGGCGa  >  3:327565/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                            
CCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTG‑TAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTGAACCTTGCGGCGA  >  NZ_CP022686/1633732‑1634007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A