Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,579 T→A 19.7% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5790TA19.7% 348.0 / 32.9 174intergenic (‑1/‑157)yhjY/tagautotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
Reads supporting (aligned to +/- strand):  ref base T (108/30);  new base A (2/34);  total (110/64)
Fisher's exact test for biased strand distribution p-value = 3.95e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460444‑2460713
                                                                                                                                        |                                                                                                                                      
tgtgTAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGCTTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGAGCATTAacc                                                                                                                                    >  4:369035/1‑139 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAacc                                                                                                                                    >  6:443505/1‑139 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAacc                                                                                                                                    >  1:900652/1‑139 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTAGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTaaaa                                                                                                                                    >  5:633154/1‑138 (MQ=255)
tgtgGAATGTACGGGTTGCGTATATACGATATATTCTTGTTGCCAGGCGTAAGCGGTGTTGAGAAAAAAGGCACTGATTACAACGCTCGCTAGCAGGCTTAGCTGCAAGCGACAAGGC‑ATTTTTTTTATGATCCTTAaca                                                                                                                                    >  6:373801/1‑140 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAacc                                                                                                                                    >  8:756550/1‑137 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAaca                                                                                                                                    >  7:733440/1‑138 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGGTGTTTTGAGAAAAAAGTCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTAATGATCATTAACACGCCt                                                                                                                               >  7:277695/1‑138 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAACGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                             >  2:893631/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                             >  5:352490/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                             >  1:126735/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                             >  1:810803/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTGAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACCCGACTcc                                                                                                                             >  3:410920/1‑140 (MQ=255)
        gTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCa                                                                                                                            >  4:101099/1‑140 (MQ=255)
         tCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                             >  7:864545/1‑138 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                          >  1:57874/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                          >  5:813033/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                          >  1:543827/1‑140 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAGAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                          >  8:243617/1‑138 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGa                                                                                                                        >  5:763816/1‑140 (MQ=255)
              gTTGCGTTTCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCACGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGa                                                                                                                        >  7:242475/1‑138 (MQ=255)
              gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGa                                                                                                                        >  7:242472/1‑138 (MQ=255)
                ttgttATCAACTTTATATTTTTGTTGCCTGGTGTATTGTGTTTTTATAAAAACGTAATTTATTCCCACGCTGGCATGCTGTCTTAGCGGCCCGGTACCACCG‑ATTTTTTTTATGAACATTAACACGACTCCAGGGAagag                                                                                                                    <  4:661678/136‑1 (MQ=255)
                  cGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CATTTTTTTATGATCATTAACACGACTCCAGGGAAGAGcc                                                                                                                  >  3:513688/1‑140 (MQ=255)
                   gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGCCAAGAGccc                                                                                                                 >  6:872864/1‑139 (MQ=255)
                    tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGa                                                                                                                >  3:815218/1‑140 (MQ=255)
                          ctttatatTCTTGTGGCCCGGCGTAGGGTGTGTTGATAAAACCGCCACTGATAACCCCCCGCGCACGCCGGCTTCGCTCCCAGCCCCCCCCG‑CTTTTTTTTATGATCATAACCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  4:213731/137‑1 (MQ=255)
                          cgttatatTCTTGTTGCCTGGCGTAAGCTGTGTTGATAAAAAAGGCACTGATTACCACGCTCGCATGCAGGCTTAGCTGCCAGCGACCACCT‑CTTTTTTTTATGATCATTAACACGACACCAGGGAAGAGCCGAATTCgg                                                                                                          <  3:73825/137‑1 (MQ=255)
                            atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                        >  4:208901/1‑140 (MQ=255)
                            atatatTAGTGTTGCCCGGGGCCAGGTGTGTTGTAAAAAACTCCACTTATAACCACCCGCGCCAGCTGGCTTTGCTCCCCGCGACCCCCG‑ATTTTTTTTTTGATAATTCCCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  8:345175/138‑1 (MQ=255)
                            aaaaatTCTTGTTCCCCGGGTTAAGCTGTGTTTAGAAAAACGCCACTGATACCCACGCTCCCACGCAGGCTTACCTGCCACCCACCCCCG‑CTTTTTTTTATGACAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                        <  4:614053/136‑1 (MQ=255)
                             tatatTCTTGTTGCCCGGCGTATGGTGTGTTTATAAAAAAGGCACTGATAACCCCGCACCCACGCAGGCTAAGCTGCCAGCTACCCCCG‑CTTTTTTTTATTATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCaa                                                                                                       <  2:126735/140‑1 (MQ=255)
                              atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                        >  8:128068/1‑138 (MQ=255)
                                  tgttgttgCCAGGCGTATGGTGTTTTTACAAAAAAGGCACTGATCACCCCGCTCCCACGCCGGCTTCGCTGCCAGCGACCCCCG‑CTTTTTTTTATGATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAAtatta                                                                                                  <  6:763816/138‑1 (MQ=255)
                                  tCTTGTTGCCAGGCGTACGGTGGGTGTCTAAAAACGCCACTGATTCCCCCGCTCGCCTGCTGGATGCGCGGCCAGCGACCCCGG‑CTTTTTTTTATTCTCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAAtatta                                                                                                  <  4:410920/140‑1 (MQ=255)
                                    ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                >  4:590212/1‑140 (MQ=255)
                                      gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              >  5:684214/1‑140 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCCCTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATACGGCAAAATTTTAGTc                                                                                             >  2:445555/1‑140 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  2:501243/1‑140 (MQ=255)
                                        tgCCTGGCGTAAGCTGTGTTGATAAAAAAGGCACTTATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATTATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  6:684214/140‑1 (MQ=255)
                                        tgCCTGGCGTAAGCGGTGTTGATAAAAAAGCAACTGATAACCCCGCGCGCAAGCAGGATTAGCTGCCCGCCACCACCT‑ATTTTTTTTATTATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  4:743015/140‑1 (MQ=255)
                                        tgCCAGGCGTAAGCGGTGTTTACAAACATCGCACTGATTACCCCGGCCGCACGCAGGCTTCGCTCCCCGCGACCCCCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              <  8:587021/138‑1 (MQ=255)
                                        tccctggCGTATGGTTTGTTGATAAAAATGGAACTTATAACCCCGCTCGCTTGCCGGCTTAGCTGCCTGGGACCACGT‑TTTTTTTTTTTTATAATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  4:301061/135‑1 (MQ=255)
                                         gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCAAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  5:481740/1‑140 (MQ=255)
                                           cAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACGCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  7:459339/1‑138 (MQ=255)
                                              gCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACCCGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGACATTGt                                                                                        >  8:566542/1‑138 (MQ=255)
                                                      ttagtTGATAAAATGCGCATTTATTCCCACCCTCTCAACCAGGCTTAGCTTCATGCTACCACCG‑CTTTTTTTTATTATAATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                <  8:115316/135‑1 (MQ=255)
                                                      ggtttttagaAAAAAGCATCTTATAACCACGCCCTAATGCTGTCTAAGCTGCCAGCCACCCCCT‑TTTTTTTTTATTATAAATAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                              <  5:427916/133‑1 (MQ=255)
                                                        tgtTGAGAAAAACGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                            >  6:121599/1‑139 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTGTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGGCATTGTATGAAGGATATc                                                                            >  6:668157/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                            >  1:189093/1‑139 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  2:186991/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  4:310454/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  1:489748/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  3:801510/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  2:260499/1‑140 (MQ=255)
                                                        agtTGAGAAAAAAGGCACTGATAACCACGCTCGCAAGCAGGCATAGCTGCCAGCGACCACCG‑CTTGTTTTTATGATCATTAACACGACTCCAGCGAAGAGCCGAATACGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  5:664215/2‑140 (MQ=255)
                                                         gtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           >  1:719876/1‑140 (MQ=255)
                                                          ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGCTCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGTatata                                                                            >  7:812656/1‑137 (MQ=255)
                                                          ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                            >  8:32645/1‑137 (MQ=255)
                                                           ttataaaaacGGCACTGATTCCCACGCGCCAAAGCAGTCTTAGCTGCCAGCTACAACCG‑TTTTTTTTTATTATAATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  4:86853/136‑1 (MQ=255)
                                                           tGATAAAAACGGCACTTATTACCACGCTCGCATGCAGGCTTAGCTGCCAGCCACCACCG‑TTTTTTTTTATGATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  3:590212/140‑1 (MQ=255)
                                                           tGACAAAAACGGCACTGATTACCCCGCTCGATAGCAGGCTTTGCGGCCCGCTACCCCCG‑ATTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  4:328232/140‑1 (MQ=255)
                                                               aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAta                                                                     >  4:52499/1‑140 (MQ=255)
                                                                aaaacGGCACTTATTCACCCGCTCCAAACAAGGGTTAGCTGCATCCTACCACCG‑CTTTTTTTTATTATCATAAACACGACTCCAGGTAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                    <  1:33443/140‑1 (MQ=255)
                                                                aaaaaGTCACTGTTTACAACTCTCTAACAAATGCTTCGCTGTCAGCAACAACCG‑CTTTTTTTTATTATCATTAACAAGAATCCAGTGAAGAGCCGAATTCTTCAATATTATTGTCATTGTATGAAGGATATCTGTCAtag                                                                    <  6:485254/140‑1 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAAATCGGCAATATTATTGTCATTGTATGAAGTATATCGGGCAtagta                                                                  >  2:775003/1‑140 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTGTTTTTTATGATAATTAACACGACTCCAGGGAAGAGCCGAATTCGGAAATATTATTGTAATTGTATGAAGGATATCGGGCAtcgta                                                                  >  2:566/1‑140 (MQ=255)
                                                                    aGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                  >  7:633393/1‑138 (MQ=255)
                                                                    aGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGc                                                                >  6:572999/1‑140 (MQ=255)
                                                                     ggCACTTATTACCACGCTGCCAAGCTGCATTATCTGCCGGCGAACCCCG‑TTTTTTTTTATCATAATTAACACGACTCCAGTGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  4:943500/140‑1 (MQ=255)
                                                                     ggCACTTATAACCACGCTCGAAAGATGGCTTTGCTGCCAGCCACAACCG‑TTTTTTTTTATTATAATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  3:33891/140‑1 (MQ=255)
                                                                     ggCACTGATACCCACGCCCCCTCGCCGGCTTCGCTGCCAGCCACCCCGG‑CTTTTTTTTTTTTCAATTACCACGACTCCAGGGAAGATCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  6:47526/140‑1 (MQ=255)
                                                                     cgCACTTATTCCCACGCTCGCACGCTGCCTTAGCTCCCAGCCACCACCG‑CTTTTTTTTATTATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  1:880196/139‑1 (MQ=255)
                                                                       cACTGATACCCACGCGCGCAACCCGGCTTAGCTGCCAGCGACCACCG‑ATTTTTTTTATCAAAATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCt                                                             <  4:175617/140‑1 (MQ=255)
                                                                            atacccACGCTCCCACGCTGGCTTCGCTGCCAGCGACCACCG‑CTTTTTTTTATCATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAtt                                                        <  2:574182/136‑1 (MQ=255)
                                                                            atacccACGCCCCCACGCCGGCTTAGCTGCCAGCGACCCCCG‑CTTTTTTTTATCATAAATACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAtt                                                        <  3:101099/136‑1 (MQ=255)
                                                                            ataaCCCCGCACCCACGCTGGCTCAGCTCCCCCCGACCCCCC‑CTTTTTTTTATGATCATACCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGtttt                                                        <  6:283724/137‑1 (MQ=255)
                                                                            aTTCCCCCGCTCCCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAtt                                                        <  2:189093/140‑1 (MQ=255)
                                                                                    ccTCGCAAGGAGGCTTAGCTGCCACTCACAAACGATTTTTTTTTATCATCTTTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAata                                                   <  8:513770/137‑1 (MQ=255)
                                                                                     cTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGa                                               >  2:691322/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  6:902342/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCATTATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  4:822195/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGaa                                              >  3:865570/1‑139 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  1:112221/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAAATCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATCGTAGCACAGTATTAATTATTGAc                                              >  6:902345/1‑140 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTAGGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCTTAGTAGCCCTGTATTAAATATTGAc                                              >  8:764507/1‑138 (MQ=255)
                                                                                                  ttcgcggCCAGCGACACCCG‑TTTTTTTTTATTATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                  <  3:282224/134‑1 (MQ=255)
                                                                                                  ttAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTGATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGACTTTTTCAACGAt                                  >  6:813765/1‑140 (MQ=255)
                                                                                                      cTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGTTATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGt                              >  2:555994/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  2:544061/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  2:29926/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCAAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGAATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  3:19740/1‑140 (MQ=255)
                                                                                                            gCGACCACCG‑CTTTTTTTTATGATCATTACCTCGACTCCAGGGAACAGCCGAATTCGGCAATATTATGGGCAGAGTAGGATTGATATCGTGCATAGTAGCACTGTATTAAATATTGACTTTTTCACCGCTGCGTCAAg                          >  7:124902/1‑138 (MQ=255)
                                                                                                            gCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTAATTGTATGAAGGAAATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  7:160789/1‑138 (MQ=255)
                                                                                                                               ttttaacATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa      <  2:709328/134‑1 (MQ=255)
                                                                                                                                 tGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa      <  8:347947/138‑1 (MQ=255)
                                                                                                                                   aactttAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  3:286922/136‑1 (MQ=255)
                                                                                                                                   aTCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  5:408629/140‑1 (MQ=255)
                                                                                                                                   aTCATAAACACGACTCCAGGGCAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg    <  8:500447/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460444‑2460713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A