Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,581 A→C 16.3% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5810AC16.3% 267.2 / 11.1 164intergenic (‑3/‑155)yhjY/tagautotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
Reads supporting (aligned to +/- strand):  ref base A (93/40);  new base C (2/29);  total (95/69)
Fisher's exact test for biased strand distribution p-value = 4.05e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

TCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATC  >  NZ_CP022686/2460442‑2460714
                                                                                                                                           |                                                                                                                                      
tctGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTTATGAGCATTaa                                                                                                                                        >  8:11133/1‑140 (MQ=255)
  tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑cc                                                                                                                                     >  2:819325/1‑139 (MQ=255)
  tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                     >  1:257119/1‑140 (MQ=255)
  tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGATTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                     >  5:371010/1‑140 (MQ=255)
   gtgGATGGTCGGGGTTGGGTATCAAGTATATATTTTTTTTGCCTGGCGTATGCTTTGTTTATAAAAACGGCACTTATTACCACGCTCGCAAGATGGCTTAGCTGCCAGCGACCACCGTTTTTTTTTATTATAATTAa‑cac                                                                                                                                    <  8:860556/140‑1 (MQ=255)
    tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                     >  3:284509/1‑138 (MQ=255)
      gAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACg                                                                                                                                   >  4:817943/1‑138 (MQ=255)
       aaTGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTATATGATCATTAA‑CACGACt                                                                                                                                >  1:102996/1‑140 (MQ=255)
         tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGAcg                                                                                                                                >  4:898487/1‑137 (MQ=255)
         tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACt                                                                                                                                >  4:1116931/1‑138 (MQ=255)
         tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTcc                                                                                                                              >  7:566285/1‑140 (MQ=255)
            ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAgg                                                                                                                           >  5:747399/1‑140 (MQ=255)
              gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAgg                                                                                                                           >  3:1072521/1‑138 (MQ=255)
              gggTTGCGTATCAACGATATATACTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACAGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCTATTTTTTATTATCATTAA‑CACGACTCCAGGGa                                                                                                                         >  8:794822/1‑140 (MQ=255)
                    cGTATCAACGATATATTCTTGTTGCCAGGACTAAGCTGTGTAGAGAAAAAAGCCACTGATTACCACGCTCGCAAGCAGGATTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑AACCACTCCAGGGAAGAGcc                                                                                                                   >  1:10078/1‑140 (MQ=255)
                     gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCg                                                                                                                  >  8:209606/1‑140 (MQ=255)
                     gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATAATTAA‑CACGACTCCAGGGAAGAGCCg                                                                                                                  >  7:336388/1‑140 (MQ=255)
                       accAACTAAATATTCTTTTTGCCAGGCTTAAGGGGTTTTGATAAAAACGCCACTTATTACCCCGCTCGCAACCAGGCTTAGCTCCCCGCCACCACCGATTTTTTTTATTATAATAAC‑CACGACTCCAGGGAAGAGCCGaa                                                                                                                <  6:508002/138‑1 (MQ=255)
                       aTCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCg                                                                                                                  >  3:640230/1‑138 (MQ=255)
                         aaaCGATATATTCTTGTTGCCAGGCGTACGCTGTGTCGACAAAAAAGCCACTGAATCCCACGCTCGCAAGCTGGCTTAGCTGCCAGCGACCCCCGATTTTTTTTATGATCATAAC‑CACGACTCCAGGGAAGAGCCGAAtt                                                                                                              <  2:585408/139‑1 (MQ=255)
                            ctatatatTCTTGTTGCCCGGCGTAAGCTGTTTTGACAAAAAAGGCACTGATAACCCCGCGCCCAAGCCGGCTTCGCTGCCCGCCACCACCGCTTTTTTTTATGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                           <  2:257119/138‑1 (MQ=255)
                              atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGCGCCGAATTCGGCa                                                                                                         >  6:457390/1‑140 (MQ=255)
                              atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                         >  2:736075/1‑140 (MQ=255)
                              atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                         >  2:974375/1‑140 (MQ=255)
                              atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                         >  2:624958/1‑140 (MQ=255)
                                atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                         >  4:402805/1‑138 (MQ=255)
                                atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGAATACCACGCTCGCAAGCAGGCTTAGCTGCCCGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                         >  4:269403/1‑138 (MQ=255)
                                       tgttgCCAGGCGTAAGCTGTGTTGAGAAAAATGGCACTGAATACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGGAATATTATTg                                                                                                >  6:516989/1‑140 (MQ=255)
                                       tgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTg                                                                                                >  1:1106380/1‑140 (MQ=255)
                                        gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTgc                                                                                               >  1:1171277/1‑139 (MQ=255)
                                        gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                               >  7:814586/1‑140 (MQ=255)
                                        gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAAGTCGGCAATATTATTGt                                                                                               >  2:996315/1‑140 (MQ=255)
                                        gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTAAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAAGATTATTgg                                                                                               >  2:377103/1‑139 (MQ=255)
                                          tgCGAGCCGAAAGTTGTGTTGATAAAATCGGCACTTTTTACCCCGCTCGCACGCAGGAGTCGCTGCCTGCTACCACCTGTTTTTTTTTTGATCATACA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                               <  4:1075325/138‑1 (MQ=255)
                                          tgCCCGGCTTCAGCTGTGTTTATAAAAACGGCAGTTATTCCCCCGCTCGCATGCAGGCTTAGCGCCCAGCGACCACTGCTTTTTTTTTTGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                             <  8:573377/140‑1 (MQ=255)
                                          tgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTACCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCTGCAATATTATTGt                                                                                               >  4:706551/1‑138 (MQ=255)
                                          tccccggCGTACGCTGTGGTGATAAAAATGGCACGTATTCCCACGCTCCCAAGCTGTCTTAGCGGCCAGCCACCACCTCTTTTTTTTTTGATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                             <  8:454442/135‑1 (MQ=255)
                                          ggCCTGGCGTACGGTGTGGTGATAAAAACGGCACTTTTACCCACGCGCGCAAGCTGGCTTAGCTGCCTCCTACCACCGCTTTTTTTTATGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                             <  8:144707/139‑1 (MQ=255)
                                           gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                              >  4:418174/1‑138 (MQ=255)
                                           gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                            >  6:334660/1‑140 (MQ=255)
                                           gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                            >  7:1020643/1‑140 (MQ=255)
                                                  gTAAGGTGTGTTTATAAAAAAGCCACTTATAACCACGCTCGAAAGCCGGCTTAGCTGCCAGCGACCACCGTTTTTTTTTATTAT‑ATTAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGa                                                                                     <  8:240937/140‑1 (MQ=255)
                                                   tAAGCTGTGTTGACAAAAAAGCCACTTATACCCACGCTCGCAAGCCGGCTTCGCTGCCAGCGACCCCCGCTTTTTTTTTTGAAAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGaa                                                                                    <  5:901721/140‑1 (MQ=255)
                                                        tttgtTTATAAAAACGTCATTGTTTCCCTCGCTCTCAAGCTGGTTTAGCTGCACTCGACCACCGTTTTTTTTTTTGATCATTCC‑CACGACTCCAGGGAAGAGCCGAATTCGGCATTATTATTGTCATTGTATGAAGGata                                                                               <  2:972474/138‑1 (MQ=255)
                                                        tgtgTTTATAAAAAAGCAAATGAATCCCCCGGGCGCACGCAGGCTTAGCTGCCAGCCACCCCCGATTTTTTTTTTGAAAAAAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                               <  1:736355/140‑1 (MQ=255)
                                                        tgtgTTGAGAAAAACGGCCCGGATTCCCACGCCCGCACCCCGGCTTCGCGGCCAGCGACCACCGGTTTTTTTTATGATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                 <  4:174289/138‑1 (MQ=255)
                                                         gttttGAGAAAAACGGCACTCATTCACCCGCACGAAAGTCGGCTTAGGGTCCTGCCACCAACTATTTTTTTTATCTAAATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatat                                                                              <  5:933840/137‑1 (MQ=255)
                                                          tgtTTATAAAAACGCCACTTATAACCCCGCTCCCAAGCAGGCTTAGCGGCGCGCCCCCCCGGTTTTTTTTTATTATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                               <  3:175320/138‑1 (MQ=255)
                                                          tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CCCGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                             >  7:559835/1‑140 (MQ=255)
                                                          tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACTCCTCCAGGGAATCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                             >  8:322498/1‑140 (MQ=255)
                                                          tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                             >  6:908667/1‑140 (MQ=255)
                                                          tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                             >  8:643854/1‑140 (MQ=255)
                                                          tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                             >  1:931761/1‑140 (MQ=255)
                                                          tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTGTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGTATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                             >  5:235767/1‑140 (MQ=255)
                                                            ttGAGAAAAAAGGCTCTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                             >  4:288719/1‑137 (MQ=255)
                                                            ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCgg                                                                           >  1:353458/1‑140 (MQ=255)
                                                             ttataaaaatGGCACTTATTACCACGCTCGCAAGGTGGATTCGCTCCCAGCGACCACCGTTTTTTTTTTTTATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                          <  7:291631/136‑1 (MQ=255)
                                                             tcataaaaacGGCACTTATTACCACGCTCTCAAGCTGGCTTAGCCGCCAGCTACCACGGTTTTTTTTTATTATATTAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                          <  1:370709/136‑1 (MQ=255)
                                                             tGAGAAAAAAGGAACTGATAACCCCGCTCCCAAGCAGGCTTAGCTGCCTGCGACCACCGCTTTTTTTTATTATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                            <  4:640230/138‑1 (MQ=255)
                                                             tGACAAAAAAGGAACTGATTACCACGCTCGCAAGCAGGCTTAGCGCCCAGCGACCACCGCTTTTTTTTATTAGCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                            <  4:549545/138‑1 (MQ=255)
                                                             gtacaaaaacGCCACGTATTCCCCCGCCCGCAAGCTGGCTTAGCTGCCAGCGACCACGGCTTTTTTTTATTATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                            <  4:358666/134‑1 (MQ=255)
                                                               agaAAAACGGACCTGATTCCCACGCGCGCAATCTTGGTTCGCGGCCACCCACCACCGCTTTTTTTTTTGAAAATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                        <  6:2724/140‑1 (MQ=255)
                                                                 aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                        >  3:918041/1‑138 (MQ=255)
                                                                  aaaacGGCAGTGATTCCCACGCTCGCAAGCTGGCTTAGCGGCCAGCGACCACCGTTTTTTTTTATGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                       <  3:817943/138‑1 (MQ=255)
                                                                    aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtc                                                                   >  7:181578/1‑139 (MQ=255)
                                                                       tgctcttaTTCCCACCCTCCCACGCAGGTTTTGCTGCAAGGCACCAGCGTTTTATTTTTAGAAAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                <  5:223976/133‑1 (MQ=255)
                                                                       ggCAGTTATAACCACGCCCGCAACCTGGCTTCGCCCCCAGCGACCACCGTTTTTTTTTATGAACAAAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                  <  3:55594/138‑1 (MQ=255)
                                                                       ggCAGTGATTCCCACGCTCGAAAGCTGGCTTTCGTGCCAGCTACCACCTTTTTTTTTTTTTATAATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                <  8:566285/140‑1 (MQ=255)
                                                                       ggCACTGATTCCCCCGCGCGCAAGCTGGTTTCGCGGCCAGCGACCACTGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                <  6:856553/140‑1 (MQ=255)
                                                                       gccACTAATTACCCCGCTCCAAAGCAGGCTTAGCCGCCCCCTACCACCGATTTTTTTTATGATCATTCC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                <  1:56526/138‑1 (MQ=255)
                                                                       ctcaattaTTACCACGCTCTCAAGATGGCTTTCATGCCAGCCACCACCTTTTTTTTTTATTTTCTTTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                <  2:852659/133‑1 (MQ=255)
                                                                         ctcTGATAACCACGCGCCCACGCTGGATCAGCCGCCAGCGACCCCTGGTTTTTTTTATTATCAAACA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                <  3:202120/136‑1 (MQ=255)
                                                                         cACTGATTCCCCCGCTCGCACACAGTCTTAGCTGCCTGCGACCACCGATTTTTTTTTTGATCATACA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCt                                                              <  8:9341/140‑1 (MQ=255)
                                                                              aTTACCACGCTCGAAAGCAGGATTAGCTGCCAGCGACCACCGATTTTTTTTATGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAtt                                                         <  2:168837/140‑1 (MQ=255)
                                                                                        tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                               >  2:835973/1‑140 (MQ=255)
                                                                                        tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                               >  7:832257/1‑140 (MQ=255)
                                                                                        tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTACAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                               >  1:662482/1‑140 (MQ=255)
                                                                                        tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑AACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTAGGAAGGATATCGGGCATAGTCGCCCTGGAGTAAATATTGAc                                               >  6:949996/1‑140 (MQ=255)
                                                                                          gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                               >  3:992473/1‑138 (MQ=255)
                                                                                          gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                               >  3:881086/1‑138 (MQ=255)
                                                                                          gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGGATTAAATATTGAc                                               >  4:85229/1‑138 (MQ=255)
                                                                                                  gCTTAGCTGCCAGCTACACCCTTTTTTTTTTTTTATCTTAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                     <  8:395113/140‑1 (MQ=255)
                                                                                                  gCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCGGTATTAAATATTGACTTTTTCACCg                                     >  2:1066063/1‑140 (MQ=255)
                                                                                                    ttAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                     >  3:575997/1‑138 (MQ=255)
                                                                                                      aGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                 >  1:454349/1‑140 (MQ=255)
                                                                                                        cTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCGGTATTAAATATTGACTTTTTCCCCGATGc                                 >  4:141812/1‑138 (MQ=255)
                                                                                                           ccAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCaa                            >  6:757874/1‑140 (MQ=255)
                                                                                                            cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAATGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCCCCGATGCGTCAAg                           >  1:362158/1‑140 (MQ=255)
                                                                                                            cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                           >  6:414557/1‑140 (MQ=255)
                                                                                                             aGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGa                          >  2:65321/1‑140 (MQ=255)
                                                                                                                     ccGCTTTTTTTTATGATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCATGAAAAGCg                    >  3:353608/1‑138 (MQ=255)
                                                                                                                                ttttataaTTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa       <  8:716838/133‑1 (MQ=255)
                                                                                                                                tATGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa       <  5:726325/140‑1 (MQ=255)
                                                                                                                                aaagATCTTTAC‑CACGACTCCAGGGTAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGTATATCGGGCATAGTAGACCTGTATTAAATTTTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa       <  6:186341/137‑1 (MQ=255)
                                                                                                                                  ttaTCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa       <  4:268689/136‑1 (MQ=255)
                                                                                                                                  ttaTCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg     <  7:209606/138‑1 (MQ=255)
                                                                                                                                    tcaaTTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt   <  1:1136262/137‑1 (MQ=255)
                                                                                                                                    aTCTTTAC‑CACGACTCCAGGGAAGAGCCTTATTCGGCAATATTATTGTCTTTGAATGAAGGATAGCGGGCATAGTAGCCCTGTATTAAATAATGACTTTTTCAACGATGCGGCAAGAAAAGCGGCTGAATTTTTTACggt   <  8:778642/140‑3 (MQ=255)
                                                                                                                                     taataaa‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATc  <  6:1026150/135‑1 (MQ=255)
                                                                                                                                     aaaTTAA‑AACGACTCCAGGTCAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACTATc  <  6:12357/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATC  >  NZ_CP022686/2460442‑2460714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A