Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F36 I0 R1 107 8366.7 6299831 98.3% 6192733 135.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,581 A→C 24.2% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5810AC24.2% 109.9 / 15.7 93intergenic (‑3/‑155)yhjY/tagautotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
Reads supporting (aligned to +/- strand):  ref base A (44/25);  new base C (2/22);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 2.30e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGG  >  NZ_CP022686/2460445‑2460717
                                                                                                                                           |                                                                                                                                        
gtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ac                                                                                                                                         >  4:156447/1‑138 (MQ=255)
 tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑aca                                                                                                                                        >  1:56781/1‑138 (MQ=255)
 tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑‑TTTTTTTATGATCATTA‑acac                                                                                                                                       >  1:548883/1‑138 (MQ=255)
 tgGAATGTCCGGGATGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCAGTGTTGAGAAAAAAGTCA‑ATGCTTACCACGCTAGCCAGCAGGCTTAGCTGCCAGCGACCAACGC‑TTTTTGTTATGATCATTA‑cca                                                                                                                                        >  3:281527/1‑138 (MQ=255)
      tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTcc                                                                                                                                 >  8:161688/1‑140 (MQ=255)
           gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑CCACGACTCCAGGGa                                                                                                                            >  6:294472/1‑140 (MQ=255)
           gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑CCACGACTCAAgg                                                                                                                              >  2:765937/1‑138 (MQ=255)
           gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGa                                                                                                                            >  8:408397/1‑140 (MQ=255)
             gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGa                                                                                                                            >  2:59816/1‑138 (MQ=255)
             gTTGCGTAACAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCTC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGa                                                                                                                            >  2:97822/1‑138 (MQ=255)
                 cGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGCGcc                                                                                                                      >  6:146674/1‑140 (MQ=255)
                  gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCg                                                                                                                     >  8:654991/1‑140 (MQ=255)
                    aTCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCg                                                                                                                     >  4:649771/1‑138 (MQ=255)
                    aTCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑AAACGACTCCAGGGAAGAGCCg                                                                                                                     >  4:291044/1‑138 (MQ=255)
                       aaCGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTc                                                                                                                >  8:583125/1‑140 (MQ=255)
                         ctatttatTGTGGTTGCCAGGCGAACGCTGTGTTGACAAAAAAGCCA‑CTTATTACCCCGCCCGAACGCAGGCTTCGCTGCCAGCGCCCACCGC‑TTTTTTTTATCATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTc                                                                                                                <  2:641794/133‑1 (MQ=255)
                         atatttatTTTTGTTCCCAGGCTTATGGTGTTTTCATAAAAACTCCA‑CTCATACCCCCCCCCGCACCCTGCTTTCGCTGCCAGGCACCCCCGA‑TTTTTTTTATCAACATAA‑ACACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                              <  6:216223/135‑1 (MQ=255)
                         agaaaaattattGTTGCCCCGCGTACGCTGTGTGGAGAAAAAAGCCA‑CTGCTAACCACGCTCCCAAGCAGGCTTAGCTCCCCGCACCCACCGC‑TTTTTTTTATCATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                              <  8:618275/134‑1 (MQ=255)
                           atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                            >  8:164739/1‑140 (MQ=255)
                           ataaatTCTGGTGGCCCGGCGTACGCGGTGTTGAAAAAACCGGAA‑CTTATACCCACGCTCCCAAGCAGGCTTCGCGGCCAGCGACCACCGC‑TTTTTTTTATCATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                              <  2:153008/138‑1 (MQ=255)
                             atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                            >  2:86433/1‑138 (MQ=255)
                                      ttgCCCGGCGTAAGCT‑TTTTGTAAAAAAAAGCATATTATTACCACGCTCCCCTCCAGCGTTCGCGGCCAGCTACCACCTA‑TTTTTTAT‑TTATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                <  6:645440/140‑1 (MQ=255)
                                       tgCCTGGCTTAAGGGGTGTTTAAATAAATGGAT‑CTGATAACCCCGCTCGAACCCAGCTTAAGTTGACTGCTACAAACTT‑TTTTTTTAATGAACATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                <  6:116121/140‑1 (MQ=255)
                                       tgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                  >  3:279048/1‑138 (MQ=255)
                                       ggCCCGGGGTACGGTGTGTGGACAAAAACGGCA‑CTGATTCCCCCGCTCCCAAGCAGGATTCGCGGCCAGCGACCCCGGT‑TTTTTTTTATCATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                  <  2:724689/137‑1 (MQ=255)
                                       ccccgggCGTAAGCTGTGTGCATAAAAAAGGCC‑CTGATTACCACCCGCGCAAGCTGGCTTACCGCCCAGCCACCCCCGC‑TTTTTTTTATGATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                  <  4:495753/133‑1 (MQ=255)
                                        gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                                 >  3:734381/1‑138 (MQ=255)
                                        gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TGTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGATTTCGGCAATATTATTGTCAt                                                                                               >  6:534272/1‑140 (MQ=255)
                                          cAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                               >  1:681229/1‑138 (MQ=255)
                                                tatgCTGTGTTGAAAAAAAAGCCA‑CTTTTAACCACGCTCGCATCCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATg                                                                                         <  3:449673/135‑1 (MQ=255)
                                                     tgtgTTTATAAAAAAGGCA‑ATTATTACCACGCTCGCACGCAGGCTTAGCTCCCAGCGACCACCGC‑TTTTTTTTATTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                    <  1:567550/138‑1 (MQ=255)
                                                     tgtgTTGAGAAAAAAGCCA‑CTTATTACCCCGCTCGAAAGCAGGCTTTCGTGCCTGCGACCACCGG‑TTTTTTTTATGATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                                  <  6:396618/140‑1 (MQ=255)
                                                       tgtTGATAAAAACGTCA‑CTCTTAACCCCGCTCTCACCCCGGATTAGTGGCCAGCGACCCACGC‑TTTTTTTTTTTTAATTTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                <  6:743998/140‑1 (MQ=255)
                                                       tgtTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGC‑TTTTTATTATGATCATTA‑ACACGACTCCAGGGAAGAGACGAATTCGGCAAAATTATTGTCATTGTATGAAGGatata                                                                                >  7:214560/1‑139 (MQ=255)
                                                       tgtTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                                >  5:396618/1‑139 (MQ=255)
                                                       tgtTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                >  6:43316/1‑140 (MQ=255)
                                                       tgtTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCAGTA‑ACACGACTCCACGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                >  5:75812/1‑140 (MQ=255)
                                                         ttGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                >  4:636466/1‑138 (MQ=255)
                                                         ttGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACTACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                >  3:772198/1‑138 (MQ=255)
                                                         ttGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAggctgtc                                                                                >  3:495754/1‑133 (MQ=255)
                                                         ttGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                >  1:106380/1‑138 (MQ=255)
                                                          ttataaaaacGGCA‑TTTATACCCCCGCTCGCAAGCCGGTTTTGGTTCCAGCGACCACCGT‑TTTTTTTTATTATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                               <  2:549933/134‑1 (MQ=255)
                                                          ttataaaaacGCCA‑CTTTTAACCCCGCTCCCACGCTGTGTTCGCTGCCAGCCACCACCGT‑TTTTTTTTATTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                             <  5:431214/136‑1 (MQ=255)
                                                          ttataaaaaaGGCA‑CTTATAACCACGCTCGCACCACGGCTTAGCTGCCAGCCACCACCGT‑TTTTTTTTATGATCAATA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                               <  3:4761/134‑1 (MQ=255)
                                                          ttataaaaaaCGCA‑GTTATAACCACGCGCGCAAGCAGGTTTCGCGGCCAGCGACCCCCGC‑TTTTTTTTATTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                               <  3:649772/134‑1 (MQ=255)
                                                          tcacaaaaaaGCCA‑CTGATAACCACGCGCCCAAGCTGGCTTAGCTCCCCGCGACCACCGA‑TTTTTTTTATGAAAATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                             <  8:290289/136‑1 (MQ=255)
                                                          tGATAAAAACGGCA‑CTGATTCCCACGCGCCCAAGCAGGCTTAGCTCCCAGCGACCACCGC‑TTTTTTTTATGATAATAC‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                             <  8:535116/140‑1 (MQ=255)
                                                          tGAGAAAAACGGCA‑CTTATTACCACGCTCGCAAGCGGCCTTCGCTGCCAGCGACCACCGC‑TTTTTTTTATTAACATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                               <  4:195898/138‑1 (MQ=255)
                                                          tGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                               <  2:106380/138‑1 (MQ=255)
                                                            ataaaaacGGCA‑GTTAAAACCACGCACGCACGCCGGTTTCGCGGCCAGCTACCACCGT‑TTTTTTTTATTAAAATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                           <  5:84377/138‑1 (MQ=255)
                                                            agaAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTATTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGAATGAAGGATATCGGGCa                                                                           >  6:635044/1‑140 (MQ=255)
                                                              aaaaaaGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACAACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGACAATATTATTGTCATTGTATGAAGTATATCTGGCa                                                                           >  3:54620/1‑138 (MQ=255)
                                                               aaaaaGCCA‑CTTATTCCCACGCTCGCACGCCGGCTTAGCTGCAAGCTACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                          <  4:586621/138‑1 (MQ=255)
                                                                 aaaGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACCCGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                      >  5:620210/1‑140 (MQ=255)
                                                                 aaaGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                      >  6:282083/1‑140 (MQ=255)
                                                                 aaaGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTAATTGTATGAAGGATATCGGGCAtagta                                                                      >  8:202948/1‑140 (MQ=255)
                                                                    ggCA‑CTTTTTACCACGCTCGCAAGCTGGCTTAGCTCCCAGCGACCACGTC‑TTTTTTTTATTATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                   <  5:918/140‑1 (MQ=255)
                                                                    ggCA‑CTTATTACCCCGCTCCCCAGCTGGCTTCGCTCCCAGCTCCCCGCGT‑TTTTTTTTATTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                     <  1:11959/138‑1 (MQ=255)
                                                                    ggCA‑CTTAATACCCCGCTCCCACGCCGGCTTCGCTGCCAGCGACCACCTC‑TTTTTTTTATGAACATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                     <  3:122351/138‑1 (MQ=255)
                                                                    gccA‑CTTATAACCCCGCTCGCACGCTGGCTTCGCTGCCAGCGCCCACCGA‑TTTTTTTTATCATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                   <  6:110358/138‑1 (MQ=255)
                                                                    gcaa‑CTTATTCCCCCGCGCGCACGCAGCCTAAGCGTCCATCCACCCCGGC‑TTTTTTTTATGATCAATA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                   <  5:290142/137‑1 (MQ=255)
                                                                    cgCA‑CTGATAACCACGCTCCAACGCAGGCTTTGCTCCCTGCTACCACCTC‑TTTTTTTTATGTTCTTTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                   <  7:497086/139‑1 (MQ=255)
                                                                     gccaattaTTACCACGCTCGAAAGAAGGCTTACCCGCCCTCGACCACGTT‑TTTTTTTAATTATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGTATATCGGGCATAGTAGcc                                                                   <  5:446834/133‑1 (MQ=255)
                                                                      cA‑CTTATAACCACGCTCGCAACCAGGCTCCGCTGCCAGCGACCACCGA‑TTTTTTTTATGATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                   <  3:86939/138‑1 (MQ=255)
                                                                           gATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGt                                                               >  3:555470/1‑138 (MQ=255)
                                                                            ttacccACGCTCCCAAGCTGGCGTGGC‑GCCACGCAACACCACATTTTTTTTTTTATCAAAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTa                                                              <  1:239439/134‑1 (MQ=255)
                                                                                   cgcgcccAAGCTGGCTTAGCGGCCAGCGACCACCGC‑TTTTTTTTATGAACATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATAtt                                                     <  5:683770/134‑1 (MQ=255)
                                                                                   cGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAata                                                       >  3:516443/1‑138 (MQ=255)
                                                                                    gCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCACTTTATTAAATAttt                                                    >  8:732751/1‑139 (MQ=255)
                                                                                    gCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTGATGATCATTA‑ACACGCCTCCAGGGAAGAGCCGAATTCGGCTATATTATTGTCATTGTATCAAGGATATCGGGCATAGTAGCCGTGTATTAAATAttt                                                    >  5:309892/1‑139 (MQ=255)
                                                                                      tCGCAAGCAGGCTTCGCTGCCAGCGACCACCGC‑TTTTTTTTATGAAAATTA‑ACAGCACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCACTGTATTAAATATTgtc                                                  >  5:431067/1‑138 (MQ=255)
                                                                                        gCAAGCGGGGTTAGCTGCCTTCGACCACCTC‑TTTTTTTTATTTTATTTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACtt                                                <  6:700110/140‑1 (MQ=255)
                                                                                          aaGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAGTGTATGAAGTATATAGGGCAAAGTAGCACTGTATTATATATTGACtt                                                >  1:470498/1‑138 (MQ=255)
                                                                                           cgctggCTTAGCGGCCAGCGACCACCGT‑TTTTTTTTATGAT‑ATTACACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACttttt                                             <  7:542073/136‑1 (MQ=255)
                                                                                                gCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAATTATTGACTTTTTCACCg                                        >  5:2070/1‑140 (MQ=255)
                                                                                                gCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                        >  6:384276/1‑140 (MQ=255)
                                                                                                  ttAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                        >  1:138390/1‑138 (MQ=255)
                                                                                                    cgCTGCCAGCGACCACCGC‑TTTTTTTTATCATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                      <  4:451220/137‑1 (MQ=255)
                                                                                                    aGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                    >  8:610492/1‑140 (MQ=255)
                                                                                                      cTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                    >  1:257819/1‑138 (MQ=255)
                                                                                                          cAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                              >  5:400396/1‑140 (MQ=255)
                                                                                                            gCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                              >  3:44586/1‑138 (MQ=255)
                                                                                                                 caccGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAg                         >  2:549007/1‑138 (MQ=255)
                                                                                                                            ttttATTATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCTGCAATATTATAGTCATTGTATGAAGGATATCTGGCATAGTATCCGTGTATTAAATATTGACTTTTTCACCGATTCGTCAAGAAAAGAGGATGAAAt               >  4:674655/1‑138 (MQ=255)
                                                                                                                             tttATGATAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAAtttt            <  5:106185/140‑1 (MQ=255)
                                                                                                                               tattatCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa          <  5:477399/140‑1 (MQ=255)
                                                                                                                               tatcaacATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa          <  7:655564/134‑1 (MQ=255)
                                                                                                                                 tGATCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa          <  2:319185/138‑1 (MQ=255)
                                                                                                                                 ggctCATTA‑CCACTACTACCTGGAAGCGCCGCATTCGGCAATATTATTGTCATTGTATGCAGGATCTCTGGCATAGTAGCCCTGTAGTAAATATTGACTTTTTCACCTATGAGTCAAGAAAAGCGGCTGAAATTTTTa          <  4:68835/135‑1 (MQ=255)
                                                                                                                                   ctCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg        <  2:32523/137‑1 (MQ=255)
                                                                                                                                   ataaTTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg        <  1:361859/135‑1 (MQ=255)
                                                                                                                                   acatttA‑ACACGACTCCACGGAAGAGCCGAATTCGTCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTATATATTGACTTTTTCACCTATGAGTAAAGAAAAGCGGCTGAAATTTTTActta      <  8:374416/136‑4 (MQ=255)
                                                                                                                                   aacATTA‑ACACGACTCCACGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCTGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt      <  7:535518/138‑1 (MQ=255)
                                                                                                                                   aaaaTTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg        <  3:419472/135‑1 (MQ=255)
                                                                                                                                   aTCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt      <  5:43316/140‑1 (MQ=255)
                                                                                                                                   aTCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt      <  6:457218/140‑1 (MQ=255)
                                                                                                                                    ataatacacacGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt      <  7:732751/133‑1 (MQ=255)
                                                                                                                                        tA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCgg   >  2:608559/1‑138 (MQ=255)
                                                                                                                                         a‑aCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCggg  <  4:734380/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCA‑CTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGG  >  NZ_CP022686/2460445‑2460717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A