Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,581 A→C 19.9% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5810AC19.9% 222.6 / 25.6 162intergenic (‑3/‑155)yhjY/tagautotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
Reads supporting (aligned to +/- strand):  ref base A (94/33);  new base C (3/32);  total (97/65)
Fisher's exact test for biased strand distribution p-value = 1.39e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTA  >  NZ_CP022686/2460444‑2460719
                                                                                                                                          |                                                                                                                                          
tgtgTAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGCTTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGAGCATTAacc                                                                                                                                          >  4:369035/1‑139 (MQ=255)
tgtgTAATGTCCGGGTTGAGTATCAACGATATATTCTTGTAGCCAGTCGTAAGCTGTGTTGAGATATAAGTCACTGAGTACCACGCTCGAAATCATGCTTAGCTGCCAGCGCCCAACG‑CTGTTTTTTATGATCATTAaca                                                                                                                                          >  6:381164/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAacc                                                                                                                                          >  1:900652/1‑139 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAaca                                                                                                                                          >  5:801345/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCAATGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTGTTTTTTATGATCATTAacc                                                                                                                                          >  5:911539/1‑139 (MQ=255)
tgtgGAATGTACGGGTTGCGTATATACGATATATTCTTGTTGCCAGGCGTAAGCGGTGTTGAGAAAAAAGGCACTGATTACAACGCTCGCTAGCAGGCTTAGCTGCAAGCGACAAGGC‑ATTTTTTTTATGATCCTTAaca                                                                                                                                          >  6:373801/1‑140 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAaca                                                                                                                                          >  7:610647/1‑138 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAacc                                                                                                                                          >  8:756550/1‑137 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAaca                                                                                                                                          >  7:733440/1‑138 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAaca                                                                                                                                          >  7:1360/1‑138 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                                   >  5:352490/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                                   >  1:810803/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                                   >  3:65924/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                                   >  1:126735/1‑140 (MQ=255)
        gTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCa                                                                                                                                  >  4:101099/1‑140 (MQ=255)
         tCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTcc                                                                                                                                   >  7:79412/1‑138 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                                >  2:722367/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                                >  1:57874/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                                >  1:543827/1‑140 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAGAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAgg                                                                                                                                >  8:243617/1‑138 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGa                                                                                                                              >  3:753638/1‑140 (MQ=255)
              gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGa                                                                                                                              >  7:242472/1‑138 (MQ=255)
                ttgttATCAACTTTATATTTTTGTTGCCTGGTGTATTGTGTTTTTATAAAAACGTAATTTATTCCCACGCTGGCATGCTGTCTTAGCGGCCCGGTACCACCG‑ATTTTTTTTATGAACATTAACACGACTCCAGGGAagag                                                                                                                          <  4:661678/136‑1 (MQ=255)
                   gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGCCAAGAGccc                                                                                                                       >  6:872864/1‑139 (MQ=255)
                    tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGa                                                                                                                      >  3:815217/1‑140 (MQ=255)
                          ctttatatTCTTGTGGCCCGGCGTAGGGTGTGTTGATAAAACCGCCACTGATAACCCCCCGCGCACGCCGGCTTCGCTCCCAGCCCCCCCCG‑CTTTTTTTTATGATCATAACCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                                <  4:213731/137‑1 (MQ=255)
                            aaaaatTCTTGTTCCCCGGGTTAAGCTGTGTTTAGAAAAACGCCACTGATACCCACGCTCCCACGCAGGCTTACCTGCCACCCACCCCCG‑CTTTTTTTTATGACAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                              <  4:614053/136‑1 (MQ=255)
                             tatatTCTTGTTGCCCGGCGTATGGTGTGTTTATAAAAAAGGCACTGATAACCCCGCACCCACGCAGGCTAAGCTGCCAGCTACCCCCG‑CTTTTTTTTATTATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCaa                                                                                                             <  2:126735/140‑1 (MQ=255)
                              atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                              >  8:128068/1‑138 (MQ=255)
                                  tgttgttgCCAGGCGTATGGTGTTTTTACAAAAAAGGCACTGATCACCCCGCTCCCACGCCGGCTTCGCTGCCAGCGACCCCCG‑CTTTTTTTTATGATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAAtatta                                                                                                        <  6:763816/138‑1 (MQ=255)
                                  tCTTGTTGCCAGGCGTACGGTGGGTGTCTAAAAACGCCACTGATTCCCCCGCTCGCCTGCTGGATGCGCGGCCAGCGACCCCGG‑CTTTTTTTTATTCTCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAAtatta                                                                                                        <  4:410920/140‑1 (MQ=255)
                                  tCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAAtatta                                                                                                        >  1:897958/1‑140 (MQ=255)
                                     tgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAAGTCGGCAATATTATTg                                                                                                     >  6:456019/1‑140 (MQ=255)
                                      gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      >  7:823584/1‑138 (MQ=255)
                                      gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                    >  5:684214/1‑140 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCCCTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATACGGCAAAATTTTAGTc                                                                                                   >  2:445555/1‑140 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGACAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGTAAGAGCCGAATTCGGCAATATTATTGTc                                                                                                   >  2:212655/1‑140 (MQ=255)
                                        tgCCTGGCGTAAGCGGTGTTGATAAAAAAGCAACTGATAACCCCGCGCGCAAGCAGGATTAGCTGCCCGCCACCACCT‑ATTTTTTTTATTATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  4:743015/140‑1 (MQ=255)
                                        tgCCCGGCGAAAGGTGTGTTGACAAAAAAGCCACTTATTACCACGCTCGCAAGCAGGCTTAGCTCCCAGCGACCACCG‑TTTTTTTTTATGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  2:274614/140‑1 (MQ=255)
                                        tgCCAGGCGTAAGCGGTGTTTACAAACATCGCACTGATTACCCCGGCCGCACGCAGGCTTCGCTCCCCGCGACCCCCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                    <  8:587021/138‑1 (MQ=255)
                                        tccctggCGTATGGTTTGTTGATAAAAATGGAACTTATAACCCCGCTCGCTTGCCGGCTTAGCTGCCTGGGACCACGT‑TTTTTTTTTTTTATAATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  4:301061/135‑1 (MQ=255)
                                        ggCCCGGCTTACGCTGTGTTCAAAAAAACGCCACTTATACCCCCGCCCGCAACCCGGCTCAGCTCCCCCCCACCCCCG‑TTTTTTTTTATCATAATACCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  4:302065/139‑1 (MQ=255)
                                         gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                                 >  5:699180/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTGTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGGCATTGTATGAAGGATATc                                                                                  >  6:668157/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  2:935129/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                                  >  1:189093/1‑139 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  1:489748/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  6:668158/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  2:544811/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  2:186991/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATAATTAACACGACTCCAGGTAAGAGCCGAATTCGGCAATTTTATTGTCATTGGATGAAGGatata                                                                                  >  2:55821/1‑139 (MQ=255)
                                                        ggttcaaaaaaacGGCAGTTATACCCACGCGCCCAAGCAGGCTAAGCTGCCAGCGACCCCCG‑TTTTTTTTTTTGATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  <  4:152039/133‑1 (MQ=255)
                                                         gtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                                 >  1:719876/1‑140 (MQ=255)
                                                           ttataaaaacGGCACTGATTCCCACGCGCCAAAGCAGTCTTAGCTGCCAGCTACAACCG‑TTTTTTTTTATTATAATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  4:86853/136‑1 (MQ=255)
                                                           tGATAAAAACGGCACTTATTACCACGCTCGCATGCAGGCTTAGCTGCCAGCCACCACCG‑TTTTTTTTTATGATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  3:590212/140‑1 (MQ=255)
                                                               aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAta                                                                           >  4:52499/1‑140 (MQ=255)
                                                                aaaacGGCACTTATTCACCCGCTCCAAACAAGGGTTAGCTGCATCCTACCACCG‑CTTTTTTTTATTATCATAAACACGACTCCAGGTAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                          <  1:33443/140‑1 (MQ=255)
                                                                aaaacGCCACTTGTTACCCCGGCCGCACGCAGGTTTAGCTGCCAGCGACCTCCG‑CTTTTTTTTAGCATAATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                          <  5:544956/140‑1 (MQ=255)
                                                                aaaaaGTCACTGTTTACAACTCTCTAACAAATGCTTCGCTGTCAGCAACAACCG‑CTTTTTTTTATTATCATTAACAAGAATCCAGTGAAGAGCCGAATTCTTCAATATTATTGTCATTGTATGAAGGATATCTGTCAtag                                                                          <  6:485254/140‑1 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAAATCGGCAATATTATTGTCATTGTATGAAGTATATCGGGCAtagta                                                                        >  2:775003/1‑140 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTGTTTTTTATGATAATTAACACGACTCCAGGGAAGAGCCGAATTCGGAAATATTATTGTAATTGTATGAAGGATATCGGGCAtcgta                                                                        >  2:566/1‑140 (MQ=255)
                                                                   acggCACTGATACCCCCGCCCCCACCCAGGCTTAGCGCCCAGCCACCACCG‑TTTTTTTTTATTATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                       <  3:134109/138‑1 (MQ=255)
                                                                   acgccACTTAATACCCCCCGCGCACGCTGGCTTAGCTGCCAGCGCCCCCGG‑TTTTTTTTTATGATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagt                                                                         <  7:238845/134‑1 (MQ=255)
                                                                    aGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGc                                                                      >  6:572999/1‑140 (MQ=255)
                                                                     ggCACTTATTACCACGCTGCCAAGCTGCATTATCTGCCGGCGAACCCCG‑TTTTTTTTTATCATAATTAACACGACTCCAGTGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  4:943500/140‑1 (MQ=255)
                                                                     ggCACTTATAACCACGCGCCAAAGCAGCATTTGCTCCCAGCGCCCACGT‑GTTTTTTTTATGATCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  3:489563/140‑1 (MQ=255)
                                                                     ggCACTTACTACCCCGCGCCCGAGCCGGCATAGCTCCCAGCGCCCCCGT‑TTTTTTTTTTTGGGTCTTAACACGTCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAGGAAGGATATCGGGCAtagtag                                                                       <  8:515973/138‑1 (MQ=255)
                                                                     gccACTGATACCCCCGCTCGCAAGCAGGCTTAGCTGCCCGCCACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  2:510721/138‑1 (MQ=255)
                                                                            ataaCCCCGCACCCACGCTGGCTCAGCTCCCCCCGACCCCCC‑CTTTTTTTTATGATCATACCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGtttt                                                              <  6:283724/137‑1 (MQ=255)
                                                                                   ccggcGCACGCTGGCTACGCCGCCAGCGCCCCCGT‑CTTTTTTTTATAACCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATAtt                                                       <  3:361564/136‑1 (MQ=255)
                                                                                    ccTCGCAAGGAGGCTTAGCTGCCACTCACAAACGATTTTTTTTTATCATCTTTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAata                                                         <  8:513770/137‑1 (MQ=255)
                                                                                     cTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGa                                                     >  2:691322/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  6:902342/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCATTATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  4:822195/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGaa                                                    >  3:865570/1‑139 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  5:431261/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  4:191154/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  1:112221/1‑140 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGCCTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGaa                                                    >  7:123914/1‑137 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTAGGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCTTAGTAGCCCTGTATTAAATATTGAc                                                    >  8:764507/1‑138 (MQ=255)
                                                                                                  ttcgcggCCAGCGACACCCG‑TTTTTTTTTATTATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                        <  3:282224/134‑1 (MQ=255)
                                                                                                  ttAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTGATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGACTTTTTCAACGAt                                        >  6:813765/1‑140 (MQ=255)
                                                                                                    aGCTTCCAGCCCCCACCG‑CTTTTATTTATTATATTTACCACGACTCCAGGGCAGAGCCGAATTCGGCAATATTATTGTCATTGTAGGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                      <  5:63735/140‑1 (MQ=255)
                                                                                                    aGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCTCCGAt                                        >  8:254184/1‑138 (MQ=255)
                                                                                                        gCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                             <  4:326463/97‑1 (MQ=255)
                                                                                                        gCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                             >  3:326463/1‑97 (MQ=255)
                                                                                                          cAGCGCCCACCG‑CTTTTTTTTATGATCATTAACACGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCatg                                >  3:20325/1‑138 (MQ=255)
                                                                                                          cAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATgaggaaat                                >  4:542020/1‑133 (MQ=255)
                                                                                                          cAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  2:29926/1‑140 (MQ=255)
                                                                                                                               ttttaacATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  2:709328/134‑1 (MQ=255)
                                                                                                                               ttttaTCATAACCACGACTCCATGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAAtttt              <  7:410116/134‑1 (MQ=255)
                                                                                                                                 tGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  8:347947/138‑1 (MQ=255)
                                                                                                                                   ttcaaacacacGACTCCAGGGACGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATTTTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  6:900087/133‑1 (MQ=255)
                                                                                                                                   atcttaaaCACGACTCCAGGGAAGAGCCGAATTAGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  2:611936/134‑1 (MQ=255)
                                                                                                                                   aacatacacacGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  5:470792/133‑1 (MQ=255)
                                                                                                                                   aTCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  5:408629/140‑1 (MQ=255)
                                                                                                                                   aTCATAAACACGACTCCAGGGCAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          <  8:500447/138‑1 (MQ=255)
                                                                                                                                   aTCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          <  7:92213/138‑1 (MQ=255)
                                                                                                                                   aTCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          <  8:335702/138‑1 (MQ=255)
                                                                                                                                   aTCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          <  7:243617/138‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCG‑CTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTA  >  NZ_CP022686/2460444‑2460719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A