Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F2 I0 R1 233 6461.8 9239822 98.3% 9082745 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,581 A→C 21.5% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5810AC21.5% 246.5 / 24.9 169intergenic (‑3/‑155)yhjY/tagautotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
Reads supporting (aligned to +/- strand):  ref base A (89/40);  new base C (3/37);  total (92/77)
Fisher's exact test for biased strand distribution p-value = 1.78e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTA  >  NZ_CP022686/2460444‑2460719
                                                                                                                                         |                                                                                                                                           
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGATAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  1:775282/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTAATGATCATTAa‑ca                                                                                                                                          >  7:303293/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑cc                                                                                                                                          >  2:415723/1‑139 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  7:298022/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  2:644095/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  3:769437/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATAATTAa‑ca                                                                                                                                          >  2:1162922/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACCGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTGTATGATCATTAa‑ca                                                                                                                                          >  2:1134581/1‑140 (MQ=255)
 ttgtaaTGTCCGGGTTGCTTATAAACTATATATTCTTGTTGCCTGGCGTACTTTGTGTTTATAAAAAAGGCACTTATAACCACGCTACCAACCAGGCTTAGCTGCCTGCTACCACCGCTTTTTTTTATTTTAATTAc‑cac                                                                                                                                         <  8:325911/136‑1 (MQ=255)
 ttgGATTGTGCGGTTTGCTTATAAACTTTATATTTTTTTTGCCTGGCTTAAGGTGTGTTGATAAAAATGGCAATGTTAACCCCGCTCGCAAGCAGGGTTTGCTGCCAGCGACCACCGCTTTTTTTTATGATAATAAa‑cac                                                                                                                                         <  8:171492/139‑1 (MQ=255)
 ggggAATGTCCGGGTCGCGAATCAACTATATATTCTTGTTGCCAGGCGAAAGCTGTGTGGAGAAAAACGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCCACCACCGCTTTTTTTTATGATCATTAc‑cac                                                                                                                                         <  8:779047/138‑1 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  6:960627/1‑138 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  5:1121052/1‑138 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTcc                                                                                                                                   >  8:502226/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTcc                                                                                                                                   >  2:1084606/1‑140 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTcc                                                                                                                                   >  4:278912/1‑140 (MQ=255)
        gTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCTCTTTTTTTTATGATCATTAA‑CACGCCTCCa                                                                                                                                  >  3:599176/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAgg                                                                                                                                >  2:220480/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAgg                                                                                                                                >  4:156658/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCAATAA‑CACGACTCCAgg                                                                                                                                >  7:392396/1‑140 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGa                                                                                                                              >  2:978734/1‑140 (MQ=255)
              gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACAGATTTATTATATGATCATTAA‑CAAGACTCCAGGGAag                                                                                                                            >  7:660549/1‑140 (MQ=255)
                   gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCg                                                                                                                       >  4:643579/1‑140 (MQ=255)
                          ggATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCCGGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAC‑CACGACTCAAGGGAAGAGCCGAATTc                                                                                                                  <  6:1057980/137‑1 (MQ=255)
                          ctatatatTCTTGTGGCCAGGCGCAAGCTGTGTTGACAAAAACGCCACTGATAACCCCGCTCGCAAGCAGGCTTCGCTGCCCGCGACCACCGCTTTTTTTTATTATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                                <  4:806885/138‑1 (MQ=255)
                          ctataaatTCTTGTGCCCAGGCGTACGCGGTGTTCACAAAAACGGCACTCATACCCCCGCCCCCACCCCGGCTTCGCCGCCCGCCACCCCCGCTTTTTTTTATGAACATAAC‑CACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                                <  2:1040518/138‑1 (MQ=255)
                            atatttTTTTGTTCCCAGGCTTACGGTGTGTTGATAAAAACGCCACTGATACCCCCGCCCCCACGCTGGCTTAGCGCCCAGCCACCACCGCTTTTTTTTATTATCAAAAC‑CACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                                <  6:311452/138‑1 (MQ=255)
                              atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CCCGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                              >  5:391445/1‑138 (MQ=255)
                              atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                              >  5:311452/1‑138 (MQ=255)
                                atTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGGATTCGGCAatat                                                                                                          >  2:86663/1‑140 (MQ=255)
                                    ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      >  7:750207/1‑140 (MQ=255)
                                    ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      >  4:999679/1‑140 (MQ=255)
                                    ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      >  4:594187/1‑140 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTg                                                                                                     >  5:614888/1‑138 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                                   >  2:902942/1‑140 (MQ=255)
                                        ggCCAGCCGTATGCTGTGTTGACAAAAACGCCACTTATAACCAGCCTCCCACGCAGGCTTAGCTGCCAGCCACCACCGCTTTTTTTTATGATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                    <  6:1097849/137‑1 (MQ=255)
                                         gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTTATGATCATTAC‑CACGACGCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                                 >  7:310589/1‑140 (MQ=255)
                                         gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                                 >  3:707311/1‑140 (MQ=255)
                                         gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                                 >  8:740872/1‑140 (MQ=255)
                                          ccAGGCGTAAGCTGTGTTGAGAAAAACGGCACTCATTACCACGCGCGCACGCAGGATTTGCTGCCAGCGACCACCGATTTTTTTTATTATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAtt                                                                                                <  4:931205/140‑1 (MQ=255)
                                            aGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGt                                                                                              >  2:801782/1‑140 (MQ=255)
                                              gCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAAAATTATTGTCATTGTAt                                                                                            >  4:1092507/1‑140 (MQ=255)
                                                   tgCTGTGTTGATAAAAAAGGCACTGATTCCCACGCTCCCAAGCAGGCTTCGCTCCCAGCGACCACCGTTTTTTTTTATCATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAgg                                                                                       <  8:42488/139‑1 (MQ=255)
                                                   tgCTGTGTTGATAAAAAAGCCACTTATTACCACGCTCGAAAGCAGGATTAGCTGCCAGCGACCACCGCTTTTTTTTATGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAgg                                                                                       <  4:131055/139‑1 (MQ=255)
                                                      tgtgTTTAAAAAAAAGGCACTTATTACCCCTCTCCCAAGCAGTCTTAGCTGCCAGCCACCACCGCTTTTTTTTATTATCTTTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                                    <  1:727286/140‑1 (MQ=255)
                                                      ggtgtTGAAAAAAACCCCACTTATTACCCCGCCCCCACGCAGGATTAGCTGCCCGCCACCACCGTTTTTTTTTATTATAATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                      <  6:614888/137‑1 (MQ=255)
                                                       gtgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatat                                                                                   >  4:723359/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGATCCGAATTCGGCAATATTATAGTCATTGTATGAAGGatata                                                                                  >  8:135688/1‑139 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  7:136704/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  8:708292/1‑140 (MQ=255)
                                                         gtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                                 >  2:381591/1‑140 (MQ=255)
                                                          ttcacaaaaacTGCACTGATTCCCCCGCGCGAATCCTGGCTTAGCTGCCAGCGACCACCGTTTTTTTTTATTATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCgg                                                                                <  4:572289/135‑1 (MQ=255)
                                                          ttGAGAAAAAAGGCAATGATTACCACGCTCTCAAGCAGGATTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTTTATGAAGGATATc                                                                                  >  6:329045/1‑138 (MQ=255)
                                                           ttataaaaaaGCCAGTTATTCCCCCGCGCGCACGCCGGGTTAGCTCCCAGCGACCCCCGCTTTTTTTTATTCTAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  2:816569/136‑1 (MQ=255)
                                                           ttacaaaacaGCCACTTATTCCCACGCGCGCAAGCAGGCTTCGCTGCCAGCTACCACCGCTTTTTTTTATTATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  2:402367/136‑1 (MQ=255)
                                                           ttaaaaaaaaGCCACTGATAACCACGCGCGCAAGCCGGCTTAGCGCCCAGCGACCACCCGTTTTTTTTATTAACATTCA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  7:804919/136‑1 (MQ=255)
                                                           tcacaaaaatGCCACTTATACCCACGCCCCCACCCCGGCTTTCCTGCCCGCGACCACCGGTTTTTTTTATGATACTAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  3:1050245/136‑1 (MQ=255)
                                                           tGAGAAAAACGGCACTTATTACCACCCTCCCAAGCTGGCTTCGCTGCAATCGACCACCGTTTTTTTTTTTTATAATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  8:930157/140‑1 (MQ=255)
                                                              aaaaacaGTCAGTTATTACCCCGGACGCTCGCAGGCTTCGGTGACAGCGACCAGCGTTTTTTTTTATGA‑AATTAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                            <  3:550240/139‑1 (MQ=255)
                                                               aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtt                                                                           >  1:802677/1‑139 (MQ=255)
                                                                aaaacGGCACTAATAACCACCCGCGCAAGCAGGCTTAGCTGCACGCCACCACTTATTTTTTTTATTATAATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                          <  3:278912/140‑1 (MQ=255)
                                                                aaaaaGCCACTTATAACCCCGCTCCCAAGCTGGCTTAGCTGCCACCCACCACCGCTTTTTTTTATTATCAAAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                            <  6:895330/138‑1 (MQ=255)
                                                                aaaaaCGCACTGATACCCCCCCCCCCAACCAGCCTTAGCTGCCACCGACCACCGTTTTTTTTTAGGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                            <  5:993105/138‑1 (MQ=255)
                                                                   aaGGCACTTATTACCCCGCTCGCAAGCAGGCTTAGCTCCCACCCACCCCCGCTTTTTTTTATTATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagt                                                                         <  5:901816/138‑1 (MQ=255)
                                                                    aGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGTCATAGTAGc                                                                      >  8:197006/1‑140 (MQ=255)
                                                                     ggatcTGATTCCCCCGCTCGCACGCTGGCTTGGCTCCCACCTACCCCCGATTTTTTTTATGATCATACA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  3:17378/136‑1 (MQ=255)
                                                                     ggCACTGATTACCACGCGCGAACGCTGGCTTCGCGGCCCCCGACCCCGGGTTTTTTTTATGATAAATAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  4:393799/140‑1 (MQ=255)
                                                                     gccACTTATTCCCCCGCTCCCAAGCAGCCTTAGCGGCCACCGACCACGGCTTTTTTTTATGCTCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  1:515925/138‑1 (MQ=255)
                                                                     gccACTTATTACCACGCTCCCAACCAGGCTTAGCTGCCAGCCACCACCGCTTTTTTTTATTATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  4:747912/138‑1 (MQ=255)
                                                                           taTTACCACGCTCGCAAGATGGATTCGCGGCCAGCGACCACCGATTTTTTTTATGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAt                                                               <  2:68067/139‑1 (MQ=255)
                                                                                   cGCCCGCAAGCCGGCTTAGCGGCCACCTACCACGGTTTTTTTTTATGATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATAtt                                                       <  8:57823/140‑1 (MQ=255)
                                                                                      tCGCAAGCAGGCTTTGCTGCCAGCGCCCACCGCTTTTATTTATGATCATTAA‑CCCGACTCCAGGGAAGAGCCGATATCGGCAATATTATTGTCATTGTATGAAGGCTATCGGGCATCGTAGACCTGTAGTATATATTGAc                                                    >  8:1001686/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CATGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  3:894626/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGGAGCCCTGTATTAATTATTGAc                                                    >  2:495848/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCGTTTTTTTATGATCATTAA‑CAAGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGTGCATAGTAGCCATGTATTAAATATTGAc                                                    >  1:1174225/1‑140 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGATGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    >  5:721999/1‑138 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGGATTAAATATTGAc                                                    >  5:496569/1‑138 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTACAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGGATTAAATTTTGAc                                                    >  6:529809/1‑138 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTATTTTATGATCATTAA‑CCCGCCTCCAGGGAAGCGCCGAATTAGGCAATAATATTTTCATTTTATGAAGGATATAGGGCATAGTAGCCCTGTTTTAAATATTGaa                                                    >  6:22176/1‑137 (MQ=255)
                                                                                           cgctggCTTCGCTCCCAGCCACCCCCGATTTTTTTTATCATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACttttt                                               <  1:145839/136‑1 (MQ=255)
                                                                                                    cgCTGCACGCGACCACCGATTTTTTTTTTGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                      <  7:539072/139‑1 (MQ=255)
                                                                                                     gCTGCCAGCTACCACCCTTTTTTTTTATGATCTTAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATg                                       <  6:669278/138‑1 (MQ=255)
                                                                                                        gCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCa                                  >  7:297538/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGCTCATTAA‑CACGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATTTTGACTTTTTCCCCGATGCGTCAAg                                >  2:19418/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  1:499400/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGATCATTAA‑AACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGAAATCGGGCATAGTAGCCCTGTATTAAAAATTGACTTTTTCACCGATGCGTCAAg                                >  8:924000/1‑140 (MQ=255)
                                                                                                            gCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  5:49080/1‑138 (MQ=255)
                                                                                                            gCGACCACCGC‑TTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGa                               >  6:286878/1‑138 (MQ=255)
                                                                                                                      cTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGa                    >  4:84821/1‑140 (MQ=255)
                                                                                                                              tattatAATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  2:1197974/140‑1 (MQ=255)
                                                                                                                              tATGATAATTAA‑CACGACTCGAGGGCAGAGGCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATATTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  8:984549/140‑1 (MQ=255)
                                                                                                                                ttaTCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  5:1069955/136‑1 (MQ=255)
                                                                                                                                tGAACATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  5:776623/138‑1 (MQ=255)
                                                                                                                                  ataaTTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  1:1111865/137‑1 (MQ=255)
                                                                                                                                  ataaTTAA‑CACGACTCCAGGGAAGAGCCGGATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  1:86663/137‑1 (MQ=255)
                                                                                                                                  aTCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          <  6:952450/138‑1 (MQ=255)
                                                                                                                                  aTCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  4:685031/140‑1 (MQ=255)
                                                                                                                                  aTCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  3:77704/140‑1 (MQ=255)
                                                                                                                                       tAA‑CACGACTCCAGGGAAGCGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCTTAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCgg     >  5:74140/1‑138 (MQ=255)
                                                                                                                                       tAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          >  6:759096/1‑133 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTA  >  NZ_CP022686/2460444‑2460719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A