Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F35 I0 R1 195 10216.4 8585726 98.3% 8439768 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,581 A→C 22.8% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5810AC22.8% 198.4 / 32.1 160intergenic (‑3/‑155)yhjY/tagautotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
Reads supporting (aligned to +/- strand):  ref base A (87/34);  new base C (3/36);  total (90/70)
Fisher's exact test for biased strand distribution p-value = 4.63e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTA  >  NZ_CP022686/2460444‑2460719
                                                                                                                                         |                                                                                                                                           
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCCCGCTCGCAAGCAGGCTTAGCTGCCAGCTACCACCGCTTTTTTTTATGATAATTAa‑ca                                                                                                                                          >  7:364059/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCAAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  3:858580/1‑140 (MQ=255)
tgtgGAATGTCCGGGTTACGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTTATGCTCATTAa‑cc                                                                                                                                          >  2:142731/1‑139 (MQ=255)
 gtgtAATGTGCGGGGTGCTTATCCATCTTATATTTTTGTTGCCTGGCTTACGGTGGGTTGATAAAATTGGCTGTGATTCCCACGATCTCAAGCAGTCTTAGGTGACATCTACCACGGATTTTTTTTATGATCATTAa‑cac                                                                                                                                         <  8:773831/140‑1 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGCGAAAAAATCCACAGATTACCACGCTCGCACGCAGGCTTAGCTGCCAGCGACCACCGATTTTTTTAATGATCATTAA‑CACg                                                                                                                                        >  2:625459/1‑140 (MQ=255)
  tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAa‑ca                                                                                                                                          >  5:879498/1‑138 (MQ=255)
    gAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACg                                                                                                                                        >  5:530328/1‑138 (MQ=255)
       tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTcc                                                                                                                                   >  1:948240/1‑140 (MQ=255)
         tCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTcc                                                                                                                                   >  6:360161/1‑138 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAgg                                                                                                                                >  2:836714/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCCACCACCGCTGTTTATTATGATCATTAA‑CACGACTCCAgg                                                                                                                                >  7:798319/1‑140 (MQ=255)
          ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGC‑TAGCTGCCCGCGACCCCCGCGTTTTTTTATTATAATTAA‑CACGACTCCAggg                                                                                                                               >  3:793055/1‑140 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGa                                                                                                                              >  1:33460/1‑140 (MQ=255)
            gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATAATTAA‑CACGACTCCAGGGa                                                                                                                              >  2:268258/1‑140 (MQ=255)
              gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAag                                                                                                                            >  8:312708/1‑140 (MQ=255)
              gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCAGTAC‑CACGACTCCAGTGa                                                                                                                              >  5:252017/1‑138 (MQ=255)
                    tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGcc                                                                                                                        >  6:1008176/1‑138 (MQ=255)
                          ttatatatTGTTGTGGCCCGGTGTCAGGTGTGATCATAAAAACGCCCCTTATACCCCCGCCCCCAAGCAGGATTTGCGGCCCGCGACCCCCCCTTTTTTTTATCATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                                <  8:140994/138‑1 (MQ=255)
                          gtatatttTATTGTTGCCCTGCGTAAGCTGTGTTCATAAAACCGCCACTTTTTACCAGGCTCGAAAGCAGTCTAAGCTGCCAGCGCCCACCGATTTTTATTATGATTATAAA‑CACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                                <  2:689646/138‑1 (MQ=255)
                            atatatTCTTGTTGCCCGGCGTAAGCTGTGTTCAGAAAAACGGCACTGATAACCCCGCGCGCAACCAGGCTTAGCTCCCCGCGACCACCGCTTTTTTTTATTATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                              <  4:1000924/140‑1 (MQ=255)
                            atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCAAGGGAAGAGCCGAATTCGGCa                                                                                                              >  4:902576/1‑140 (MQ=255)
                            atatatCCTTGTTCCCAGGCTTACTCGGTGTCTATAAAAACGCCCCGTATACCCACGCGCCCACGCAGGCTGAGCTGCCCGCCACCCCCGATTTTTTTTATTATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                              <  2:457356/140‑1 (MQ=255)
                                atTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCTGCAatat                                                                                                          >  7:695953/1‑140 (MQ=255)
                                atTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAatat                                                                                                          >  7:793637/1‑140 (MQ=255)
                                    ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTTTGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      >  4:326999/1‑140 (MQ=255)
                                    ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      >  1:449190/1‑140 (MQ=255)
                                     tgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTg                                                                                                     >  4:403321/1‑140 (MQ=255)
                                      gttgCCCGGCTTCTGCGGTGTGTATAAAAAGGGAACTGATTACCCCCCACGCACCCGGTCGAAGCTGCCAGACACCACCGATTTTTTTTATGAACATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                      <  6:711636/138‑1 (MQ=255)
                                      gttgCCAGGCGTCAGCTGTGTTGAGAACAACGGCCGTGAATCCCACGCGCCCACCCCGGATTCGCTGCCCGCTACCACCGTTTTTTTTAATTATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                                    <  4:444492/140‑1 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGta                                                                                                   >  7:552705/1‑139 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                                   >  3:641616/1‑140 (MQ=255)
                                       ttgCCAGGCGTAAGCTGTGTGGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGATGCCAGCGCCCACCGCTTTTTTTTATGATCATTAC‑CAAGCCTCCAGGGAAGAGCCGAATTCGGCAAtattatgt                                                                                                     >  5:764676/1‑136 (MQ=255)
                                        ggCCTGGCGTAAGGTGTGTTCATAAAAACGGCACTGATTACCACGCCCCCAAGCCGGCTTAGCTGCCCGCGACCACCGCTTTTTTTTATTAAAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  4:1006542/139‑1 (MQ=255)
                                        ggCCCGGGGTATGCTTTGTTTACAAAAAAGGCACTTATTACCAGGCTCGAAAGCTGGCTTCGCGGCCAGCGACCACCGTTTTTTTTTATTATCTTACC‑CACGACTCCAGTGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  4:1037265/139‑1 (MQ=255)
                                        ggCCCGGCTTAAGGTGTGTTGACAAAAACGGCACTTATACCCACCCGCCCACGCCGGATTAGCGGCCAGCCACCCCCGTTTTTTTTTATTAACATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  7:590074/139‑1 (MQ=255)
                                        gcccAGGTGTATGCTGTGTTTATAAAAAACCCTCTTATTACCACGCTCGAAACCTGTTTTAGCTGCCCGCGACACCCGATTTTTTTTTTTATAATATC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                                  <  8:83826/138‑1 (MQ=255)
                                          ccAGGCGTAAGGGGGGTTGACAAAAAAGGCACTGATACCCACGCTCGCAAGCAGGCTTAGCGGCCAGCGACCACCGCTTTTTTTTATTATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAtt                                                                                                <  2:759407/140‑1 (MQ=255)
                                            aGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGt                                                                                              >  3:246659/1‑140 (MQ=255)
                                              gCGTATGGTGTTTTCATAAAAAAGGCCCTGATAACCACGCCCGCACGCCGGTTTACCTGCCAGCCACCACCGATTTTTTTTATTATAATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAt                                                                                            <  8:559118/140‑1 (MQ=255)
                                              gCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAt                                                                                            >  7:4944/1‑140 (MQ=255)
                                              gCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTGTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATg                                                                                           >  7:195455/1‑140 (MQ=255)
                                                   aGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAgg                                                                                       >  4:522779/1‑140 (MQ=255)
                                                      ggtgtTGTCAAAAATGGCACTGATTCCCCCGCGCGAAAGATGGCTTCGCGGCCATCGACCACCCCTTTTTTTTTTGATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                      <  6:952629/137‑1 (MQ=255)
                                                      ggtgtTGATAAAAACGGCAATGATTACCCCTCTCGCACGCAGGCTTAGCGCCCAGCGACCACCGATTTTTTTTATGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                      <  6:817582/137‑1 (MQ=255)
                                                      ggtgtTCACAAAACCGCCACTTATTACCCCGCCCTCCTGCTGGCTTTGCGGCCCGCTACCACCGTTTTTTTATATCAAAATACC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                      <  5:918352/137‑1 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  1:411832/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  8:389249/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  3:354945/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                                  >  1:931630/1‑140 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCGTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                                  >  7:16372/1‑139 (MQ=255)
                                                        tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACAACCGCTTTTTTTTATGATCAATAA‑CACGACTCCAGGGAAGAGCCGAATACGGCAATATTATTGTAATTGTATGAAGGatata                                                                                  >  7:886410/1‑139 (MQ=255)
                                                        tgatGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCGTTTTTTTAGGATCATTGA‑CACGACTCCAGGGAAGAGCCGAATTCGGAAATATTATTGTCAGTGTATGAAGGATATc                                                                                  >  7:595091/4‑140 (MQ=255)
                                                         gtTGAGAAAAACGCCACTTATTACCACGCTCGCAAGCTGGCTTAGCTGCCCGCGACCCCCGCTTTTTTTTATGATATTTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                                 <  7:739285/140‑1 (MQ=255)
                                                           ttataaaaacGGCACTTATAACCACCCTCCCAAGCAGGCTTCGCTGCCCGCCACCACCGTTTTTTTTTATCATAATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  2:388778/136‑1 (MQ=255)
                                                           ttacaaaaacGGCACTGATACCCACGCTCGCAAGCAGGCTTCGCGGCCAGCGACCACCGCTTTTTTTTATTATAATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  3:266708/136‑1 (MQ=255)
                                                           tGATAAAAACGGCAATTATTACCCCGCTCCAACGCTGGCTTAGCTGCCAGCGACCACCGATTTTTTTTATTATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  2:1080606/140‑1 (MQ=255)
                                                           tGAGAAAAAAGGCCCTGATTACCCCGCGCGCAAGCCGGCTTAGCTGCCAGCGACCCCCGATTTTTTTTATTATAATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                               <  1:829229/140‑1 (MQ=255)
                                                           gtataaaaacCGCACTGATAAACACGCGCGCAAGCCGGCTTAGGGCCCCGCGACCCCCGCTTTTTTTTATCATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                                 <  6:639517/134‑1 (MQ=255)
                                                               aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGACGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                             >  6:670765/1‑138 (MQ=255)
                                                                aaaaaGGCAATGATCACCATGCTCGCTACCTGGCTTAGCTCCCAGCTACCACCTATTTTTTTTATGATCATACC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                          <  1:445842/140‑1 (MQ=255)
                                                                 aaaaGGCACTGATTACAACGCTCGCAAGCAGGCTTAGCTGCCATCGACAACCGCTTTTTTTTATGATAATTAA‑CACGAATCCAGGGAAGAGCCGAATTCTGCAATATTATTGTCATTGTATGAAGGATATCTGGCAta                                                                           >  6:439961/1‑138 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                        >  1:1024876/1‑140 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                        >  1:1080606/1‑140 (MQ=255)
                                                                  aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                        >  1:160506/1‑140 (MQ=255)
                                                                     ggaaCTCATAACCACGCTCGAAAGCTGTCTTAGCGCCCAGCTACCCCCGCTTTTTTTTTTGATCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  2:362493/137‑1 (MQ=255)
                                                                     ggCATTTATTCCCCCGCTGGCAAGCTGCATTTGCTGCCAGCTACCACCTTTTTTTTTTATGAT‑ATTAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                       <  5:297146/138‑1 (MQ=255)
                                                                     gccACTGATTACCCCGCACGCCAGATGGCTTTGCTGCCAGCGACCACGTTTTTTTTTTTTTAGCATACA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                                     <  2:517918/138‑1 (MQ=255)
                                                                      ccACTTATAACCCCGCTCGACTGCTGGCTTTGCTGCCTGCTACCACCGGTTTTTTTTATGATCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGccc                                                                    <  3:511080/139‑1 (MQ=255)
                                                                       cACTGATTACCACGCTCGCAAGCCGGCTTAGCTGCCCGCCACCACCGCTTTTTTTTAAGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCt                                                                   <  3:948509/140‑1 (MQ=255)
                                                                            aTTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCCCCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAGAGTAGCCCTGGAtt                                                              >  1:1013854/1‑140 (MQ=255)
                                                                                   cgcgcgCACGCAGGCTAAGCTCCCCGCCACCACCGATTTTTTTTTTGACCCTACA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATAtt                                                       <  7:603293/140‑1 (MQ=255)
                                                                                   cGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAata                                                         >  6:489564/1‑138 (MQ=255)
                                                                                    gCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGTGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCGTGTATTAAATATTg                                                      >  8:861474/1‑140 (MQ=255)
                                                                                      tCGCACGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGaa                                                    >  8:594882/1‑139 (MQ=255)
                                                                                      tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTGTTTTTTATGATCATTAA‑CACGCCTCCCGGGAAGAGCCGAATTCGGCGATATTATTGTCAGTGTATGAAGGATATCGGGCATAGTAGCCCGGGATTAAATATTGAc                                                    >  4:485773/1‑140 (MQ=255)
                                                                                      tCGCAAGCAGCCTTAGCTGCCAGCGACCACCGCTTTTTTATATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAAATCGGCAATATTAATCTCATTGTATGAAGGATATCGGGCATAGTAGCGCTGTATTAAATATTGaa                                                    >  4:309967/1‑139 (MQ=255)
                                                                                      gcccacgCAGGCTTTGCTGCCAGCGACCACCGGTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                                    <  4:684981/134‑1 (MQ=255)
                                                                                        gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGTAGGATATCGGGCATAGTAGCCCGGTATTAAATATTGACtt                                                  >  7:968146/1‑140 (MQ=255)
                                                                                           aGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATAAA‑CACGACTCCACGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACttttt                                               <  3:560196/140‑1 (MQ=255)
                                                                                                 tttcccTGCCAGCTACCACCGTTTTTTTTTATGACAATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGa                                         <  7:44818/135‑1 (MQ=255)
                                                                                                  ttAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                        >  2:933475/1‑140 (MQ=255)
                                                                                                  ttAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCGGTATTAAATATTGACTTTTTCACCgct                                        >  1:708106/1‑138 (MQ=255)
                                                                                                         ccAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCaa                                 >  2:646378/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  3:1026007/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  4:185366/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  2:318332/1‑140 (MQ=255)
                                                                                                          cAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  7:962840/1‑140 (MQ=255)
                                                                                                          actcGACCACCGCTATTTTATATTATAAATAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAAAGTAGCCCTGAATTAAATATTGACTTTTTCACCGATGCGTCAAg                                >  4:742559/4‑140 (MQ=255)
                                                                                                            gCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTTACTTTTTCACCGATGCGTCAAg                                >  5:673477/1‑138 (MQ=255)
                                                                                                                   ccGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCggg                       >  1:417506/1‑139 (MQ=255)
                                                                                                                              tttGAACATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  1:597863/138‑1 (MQ=255)
                                                                                                                              tattttCATAAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGTCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  4:866000/135‑1 (MQ=255)
                                                                                                                              tATGATCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa            <  2:1051424/140‑1 (MQ=255)
                                                                                                                                 gATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGa         >  1:790413/1‑140 (MQ=255)
                                                                                                                                 gATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGa         <  1:646378/140‑1 (MQ=255)
                                                                                                                                  aTCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg          <  6:264104/138‑1 (MQ=255)
                                                                                                                                  aTCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  8:757842/140‑1 (MQ=255)
                                                                                                                                  aTCATTAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  2:948240/140‑1 (MQ=255)
                                                                                                                                  aTCATAAC‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt        <  1:148435/140‑1 (MQ=255)
                                                                                                                                        aa‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTGATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTa  <  3:521017/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAA‑CACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGATCGGGTA  >  NZ_CP022686/2460444‑2460719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A