Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F35 I0 R1 157 6985.9 8006972 98.3% 7870853 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,905,221 T→G 15.2% L116R (CTT→CGT) ‡ CIW80_RS24815 → coenzyme F390 synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,905,2210TG15.2% 130.0 / 4.4 85L116R (CTT→CGT) ‡CIW80_RS24815coenzyme F390 synthetase
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base G (13/2);  total (49/36)
Fisher's exact test for biased strand distribution p-value = 1.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATCAAAG  >  NZ_CP022686/4905089‑4905350
                                                                                                                                    |                                                                                                                                 
tgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTGAAACCCTGCGGTGGGAGGGTCAGGGTTGGGGGTTAATCAGGTTAAGAGGGGCGGCGTGGGGGTTttgtt                                                                                                                            >  7:738519/1‑140 (MQ=255)
  aaTACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCCAGCGGTGGGCGGGTCAGTGTTGGGGGTTTATCATGTACATCGGGTCGTCGGGGGGGTtttttttt                                                                                                                          >  7:80464/1‑138 (MQ=255)
  aaTACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGAGCAGTGAGCAGAGTCGTGATTTCAACCCCTGGGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTttgtt                                                                                                                            <  3:949673/138‑1 (MQ=255)
    tACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTAAAACACAGAGGTTGCATGTTCAGTGTTGTGGTTTAAGAAGGTAAAACGGGGAGTGGTTGTGTTTttgtttt                                                                                                                          >  6:67523/1‑136 (MQ=255)
         cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTAAAACCCGGCGTTGGCAGGTGGAGTGGTGGGCGTTACTGAGGGAGAACGGGGCGGAGTGGGGGTTTTTTTTGTAGcc                                                                                                                     >  4:423301/1‑138 (MQ=255)
           ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTTCAACCCAGCGGTGTCAGGGTCAGTGTTTGGCGTTGATAAGGGACAACGGGTCGTCGTGGGCGttttttttttttgccg                                                                                                                   >  3:102277/1‑132 (MQ=255)
           ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                                                 <  8:77017/140‑1 (MQ=255)
           ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTTGGCAGGGTCAGTGTTGGGCTTTCATAAGGTACATCGGGTAGTGGTGGGCTTTTTTTTTTTAGcccc                                                                                                                   >  4:720063/1‑137 (MQ=255)
           ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCGGCGTTGGCAGGTTAAGTGTTGGGGTTTCATCAGGTACATAGGGTAGTAGTGGGCGTTTTGTTTTTATcccc                                                                                                                   >  2:626867/1‑137 (MQ=255)
           ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGGGAGCAGAGGCGTGATTGCAAACCCTGCGTTGGCAGGTTCAGTGTTCGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                                                 <  7:641877/140‑1 (MQ=255)
             tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                                                 <  1:535024/138‑1 (MQ=255)
             tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGGTGGGCTTGCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                                                 <  2:699267/138‑1 (MQ=255)
             tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGGCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                                                 <  5:302916/138‑1 (MQ=255)
             tttAAAGCGCGACGAACTTCGCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCATGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                                                 <  3:52779/138‑1 (MQ=255)
              ttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCGGCGGTGGCAGGGTCAGTGTTGGGCGTTGATCAGGTACATCGGGGCGTCGTGGGCTTTTTTTTGTTATCCCGCAc                                                                                                                >  4:561003/1‑138 (MQ=255)
               tAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACg                                                                                                               <  1:107661/138‑1 (MQ=255)
                  cgcgcGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGc                                                                                                            <  1:480431/137‑1 (MQ=255)
                        acgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTTGGCAGGGTCAGTGTTGGGCTTTCATCCGGTACCTCGGGTCGTCGGGGGCGTTTTTTTTTTAGCCCCCACGCCCAAccgc                                                                                                      >  4:118936/1‑135 (MQ=255)
                        acgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCAGCGTTGGCAAGGTCAGTGTTGGGCTTTAATAAGGTACATCGGGTCTTCGTGGGGTATTTGTTTTTATACCGCACGACGAACAGa                                                                                                      >  2:595394/1‑138 (MQ=255)
                             cTTCTCGATTGTGCACTGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTgg                                                                                                 <  2:1015174/138‑1 (MQ=255)
                                  cGATTGTGCTATGCGCAGTGATCATAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGccgccg                                                                                          <  8:771003/140‑1 (MQ=255)
                                  cGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGgccgc                                                                                            <  6:450523/138‑1 (MQ=255)
                                          cAATGCGCAGTGAGCAGAGTCGTGGTTTCAAACCCAGCGGGGGGAGGTTGAGGGTTGGGCGTTGATCAGGTTCATAGGGGGGTGGGGGGGGGTTTTTTGTTTGGCCCCCCCCGCCAAACATGTTGGCCGCCggggggg                                                                                    >  2:195760/1‑133 (MQ=255)
                                               cgcAGTGAGCAGAGTCGTGGGTTAAAACACGGGGTTGGCAGGGTGAGTGGTGGGGTTTCATCAGGGTCCTAGGGTCGTCGTGGGGGGTTTGTTGTTTGGCCGCACGAGGCCCAGAGGGGGGGCGGCGGGGtggtggga                                                                               >  2:265460/1‑136 (MQ=255)
                                                            gTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGAc                                                                <  7:573761/140‑1 (MQ=255)
                                                                   tttCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTTGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGatt                                                           <  1:645807/138‑1 (MQ=255)
                                                                       aaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATAAGGTAAATCGGGTCGTCGTGGGGATTTTTTTGTTAGCCCGCACGCGCAACAGATGTGGGGCGCCGGTGTGGAGGCAAAAGTGTTGCCCGACGGTTTAttt                                                       >  4:847527/1‑138 (MQ=255)
                                                                        aaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTg                                                      <  1:295437/138‑1 (MQ=255)
                                                                         aCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTTGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                                                     >  1:137089/1‑138 (MQ=255)
                                                                         aCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                                                     >  2:995875/1‑138 (MQ=255)
                                                                         aCCCTGAGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATAGGGTCGTCGTGGGCGTTTTGTTGTTAGCCCGCACGACCAACAGATGTGGTCAGCCGGGGTGCTGGCAACAGTGTTTACAGTCGGTTGATTTgg                                                     >  4:631248/1‑137 (MQ=255)
                                                                          cccTGCGTTGGCAGGTTCATTGTTTGGCTTTCATCAGTTACATCGGGTCGTCGTGGGAGTTTTGTTGTTAGCCCGCACGAGGAACAGATGTGGGCCGCCGGGGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTgac                                                    <  3:308944/138‑3 (MQ=255)
                                                                             tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGGTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGGGTGCTGGCCAAAGTGTTGCCCGGCGGTTTTTTTGCCAAagg                                               >  7:859307/1‑139 (MQ=255)
                                                                               ggTTGGCAGGTTCTGTGTTGGGCTTTCTTCCGGTACTTGGGGTCGTGGTGGGGTTTTTGTTGTTACCCCGAACGAGCAAATGTTGTGGGCCGCCGGTGTGCTGGAAAAAGTGTTGCCCGACGGTTTATTTGCCAAaga                                               <  4:1005135/137‑1 (MQ=255)
                                                                               cGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAaga                                               >  6:400724/1‑138 (MQ=255)
                                                                                  tGGCAGGTTGAGTGTTGGGCTTTCTTCAGGTTCATCGGGTCGGCGTGGGGTTTTTGTTGTTAGCCCGCACGATCATCTGATGTGGGCGGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCTAAGAGCg                                            <  2:285750/138‑1 (MQ=255)
                                                                                  tGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTTGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCg                                            <  6:370376/138‑1 (MQ=255)
                                                                                  tGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCggg                                          <  8:495794/140‑1 (MQ=255)
                                                                                     cAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGGTGCCCGACGGTTTATTTGCCAAAGAGCGGGt                                         <  5:74343/138‑1 (MQ=255)
                                                                                      aGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCCCGAGCAACAGCTGTGGGCCGCCGGTGTGCTGGCAAAATTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTg                                        >  5:960204/1‑138 (MQ=255)
                                                                                       ggTTCAGTTGTGGGCTTTCAGCAGGTAGATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATTTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCgggggg                                       <  2:621238/138‑1 (MQ=255)
                                                                                       ggTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGTCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGTTTTATTTGCCAAAGAGCGGGTgg                                       <  1:695259/138‑1 (MQ=255)
                                                                                       ggTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGgcg                                     <  7:356813/140‑1 (MQ=255)
                                                                                       ggTTCAGTGGTGGGCTTTCATCAGGTACATCGTGTCGTCGTGGGCTTGTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg                                       <  3:791335/138‑1 (MQ=255)
                                                                                           cAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCGTTTTTGTGTTAGCCCGCACGACCAACCGAAGTGAGCCGCCCGAGTGCTGGCAAAAGTGGTGCACGCCGGTATAATTGCCAAACAGCGGGtagaccg                                   >  4:5683/1‑132 (MQ=255)
                                                                                           cAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGGGGGCTTTTTGTTGTTATCCCGCACGGGCAACAGATGTTGGCCGCCGGTTTGCTGGCAAAAGTTTTGACCGCAGGTTTATTTGCCAAATAGCGGGTGGCGCt                                   >  2:932332/1‑138 (MQ=255)
                                                                                               gtTTGGCTGTCATCAGGTACATCGGGGCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGGGCGGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGttt                               <  4:111998/138‑1 (MQ=255)
                                                                                                ttGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGGTTTTTGTTGTTAGCCCGCCCGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGAAGGTTTATTTGCCAAAGAGCGGGTGGCGCGGttta                              >  1:515921/1‑137 (MQ=255)
                                                                                                ttGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGttta                              >  6:902564/1‑137 (MQ=255)
                                                                                                  gggCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGCGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGCCGGTTTATTTTCCAAAGAGAGGGTGGCGCTGGTTCTg                            >  3:609609/1‑138 (MQ=255)
                                                                                                  gggCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTgcg                          >  8:176273/1‑140 (MQ=255)
                                                                                                   ggCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTTTTTGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGGGCTGGCAAAAGTGTTGCCCGCCGGTTTATTTGGCAAAGAGCGGGTGGCGGTGTTTCTgcgc                         >  8:686410/1‑140 (MQ=255)
                                                                                                   ggCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCCACAGATGTGGGCCGCCGGGGTGCTGGCAAAACTGTTTCCCGACGGGTTTTTTTCCAAAGAGCGGGTGGCGCGGTTTATgcgc                         >  7:38899/1‑140 (MQ=255)
                                                                                                    gCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCCCGAGCAACAGATGTGGGCCGCCCGTGTGCTGGCAAAAGTTTTGCCCGACGGGTTTTTTGCCAAAGAGCGGGTGGCGCGGtttaggcg                          >  1:957895/1‑133 (MQ=255)
                                                                                                     cTTTCATCAGGTACATCGGGTCGTCGTTGGCGTTTTTTTTTTAGCCCGCCCGAGGAACAGATGTGGGCCCGCGGTGTGCTGGCAAAATTGTTGCCCGACTGTTTATTTGCCAAATAGCCGGTTGCGCTGTTTCTgcgc                         >  6:51110/1‑138 (MQ=255)
                                                                                                     cTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTTTTGTTTGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTgcgc                         >  4:685949/1‑138 (MQ=255)
                                                                                                     cTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTTTTAGCCCGCAAGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTTGCGCTGTTTCTgcgc                         >  6:594511/1‑138 (MQ=255)
                                                                                                     cTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTCGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTgcgc                         >  1:10117/1‑138 (MQ=255)
                                                                                                     cTTTCATCAGGTACATCGGGTCGTCGTGGGCGTTTTGTTGTTAGCCCGCACGAGCAACAGAAGTGGGGCGCCGGTGGGGTGGCAAAAGTGTTTCCCCACGGTTTATTTTGCAAAGAGCGGGTGGGGCGGTTTGTgcgc                         >  5:656571/1‑138 (MQ=255)
                                                                                                     cTTTCATCAGGTACATCGGGTCGTCGTGGGCGTTTTGTTGTTAGCCCCCACGAGCAACATATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTTGCAAAGAGCGGGTGGCGCTGGTTATgcgc                         >  5:195958/1‑138 (MQ=255)
                                                                                                      tttCATCCGGGACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTgcgcg                        <  2:432580/138‑1 (MQ=255)
                                                                                                      tttCATCAGGTACATCGGGGCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAACGAGCGGGTGGCGCTGTTTCTgcgcg                        <  3:355211/138‑1 (MQ=255)
                                                                                                        tcatcaGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCCGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGACAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGa                    >  8:101041/1‑140 (MQ=255)
                                                                                                          atcaGGTACATCGGGTCGTCGTGGGCTTTTTGTTTTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCCACGGTTTATTTTCCAAAGAGCGGGTGGCGCTGTTTCTGCGCCCggc                    >  6:445355/1‑137 (MQ=255)
                                                                                                          atcaGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGGGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCggc                    >  2:132794/1‑137 (MQ=255)
                                                                                                           tcaGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGac                   <  6:819220/138‑1 (MQ=255)
                                                                                                           tcaGGTACAGCGGGGCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGac                   <  2:140650/138‑1 (MQ=255)
                                                                                                             cggTCCATCGGGGCGTCGTGGGCTGTTTGTTGTTAGCCCGCACGAGCAACAGATGGGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGGTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGacaa                 <  1:162368/137‑1 (MQ=255)
                                                                                                             aGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGacaaca               <  8:803236/140‑1 (MQ=255)
                                                                                                             aGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGacaa                 <  4:367939/138‑1 (MQ=255)
                                                                                                               ttACATCGGGTCGTCGTTGGCTTTTTGTGGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGacaaca               <  6:26941/137‑1 (MQ=255)
                                                                                                               gTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGacaaca               <  6:810379/138‑1 (MQ=255)
                                                                                                                 aCATCGGGTCGTCGTGGGCTTTTTTTTGTTATCCCGCCCGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGGCCGGCGGGTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACaacaac             >  2:143845/1‑138 (MQ=255)
                                                                                                                 aCATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAACCGCGGGTGGCGCTGTTTCTGCGCGCGGACaacaac             >  6:514206/1‑138 (MQ=255)
                                                                                                                 aCATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAAAAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGGTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACaacaac             >  4:990555/1‑138 (MQ=255)
                                                                                                                 aCATCGGGTCGTCATGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACaacaac             >  3:726600/1‑138 (MQ=255)
                                                                                                                    tCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGGTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACCACAACCTg          >  3:138904/1‑138 (MQ=255)
                                                                                                                      gggTCGTCGTGGGCTTTTTGTTTTAAACCCGCAAGCGCAACAGATGTGGGCCCCCGGTGTGCTGGAAAAAGTGTTGCCCGACCGGTTATTTGGCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCCGCCAACAACCTGtt        >  6:971603/1‑137 (MQ=255)
                                                                                                                      gggTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGCCAACAACCTGTa        >  4:121788/1‑138 (MQ=255)
                                                                                                                      gggTCGTCGTGGGCTTTTTGTTGTTAGCCCCCCCGAGCAACAGATGTGGGCCCCCGGGGTGGAGGCAAAACTGTTGCCCGACCGTTTTTTTGCCAAACACCGGGTGGCGGTGTTTATGCGCGCGGACCACAACCAGtt        >  4:313219/1‑137 (MQ=255)
                                                                                                                         tcgtcgTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATCaaa   >  8:393366/1‑140 (MQ=255)
                                                                                                                         gcgtcgtGGGCGTTTTTTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATCa     <  4:604469/137‑1 (MQ=255)
                                                                                                                          cgtcgtGGGGTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATCaa    >  4:284376/1‑138 (MQ=255)
                                                                                                                          cgtcgtGGGCTTTTTTTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTTCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATaaaac  >  7:179055/1‑139 (MQ=255)
                                                                                                                            tcgtGGGCTTTTTTTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTTCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATCAAAg  >  5:266184/1‑138 (MQ=255)
                                                                                                                                    |                                                                                                                                 
TGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTCGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCTGTTTCTGCGCGCGGACAACAACCTGTATCAAAG  >  NZ_CP022686/4905089‑4905350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A