Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F2 I0 R1 122 5985.5 7364544 98.3% 7239346 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,331,940 A→C 18.8% F289V (TTT→GTT)  ybhR ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,331,9400AC18.8% 121.1 / 8.2 90F289V (TTT→GTT) ybhRABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (40/31);  new base C (2/17);  total (42/48)
Fisher's exact test for biased strand distribution p-value = 4.98e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACGCCCAG  >  NZ_CP022686/4331805‑4332079
                                                                                                                                        |                                                                                                                                           
aaaaataaatCTGCTTGTTAATGTCGGTAAAGTGGCTAATAGGGTTAATCCACGTCAGATTTTGCACCCAAATTGGCATGTTTTCCACCGGCTAAACGAAACCGAAAAGGAGAATGGCGGG‑AATCATAAAGACAAaca                                                                                                                                           <  5:601418/136‑1 (MQ=255)
   aataaatCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGcag                                                                                                                                      >  4:245781/1‑138 (MQ=255)
         tCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGGCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAAc                                                                                                                                >  3:63145/1‑140 (MQ=255)
            gCTTGTTAAGGGCCGTAATGTGGATAATAGGGAAAATCCACTTAAGATTTTGCAGCAACATTGCCATGTTTTCCACCGGCTAAACTTACCCGGAACGGATATTGGCGGG‑CACAATAAAGACAAACACGCCGATAAACGcc                                                                                                                             <  8:661563/140‑1 (MQ=255)
               tGGTAAGGTCCGTAAAGTGGCGAATAGGGTTAAGCCACGTCAGATTTTGAAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAATGACAAACACGCCGATAAACGCct                                                                                                                            >  6:332931/1‑138 (MQ=255)
                 ttAATGTCCGTAATGTGGCTATTAGGGTTATTCCTCTTCAGTTTTTGCAGCCACATTGGATTTTTTTCCACCGGCGAAACGTAACCGTAAAGGAGAATGGCGGG‑CATCAAAAAGACAACCACGCCGATAAACGCctgctg                                                                                                                        <  3:674177/139‑1 (MQ=255)
                 ttAATGTCCGTAACGTGGCTATTTGGTTTAATCCACGTCTTATTTTGCAGCCACTTTGGCATGTTTTCCCCCGGCTAAACGTACCCGGAATGGAGACTGGGGGG‑CATCATAAAGACAAACACGCCGATAAACGCctgc                                                                                                                          <  6:592145/137‑1 (MQ=255)
                 ttAAGGTCCTAAACGTGCCCAATCGGGCAAATCCACGTCAGATTTTGCAGCAAAATTGGAATGTTTACCCCCGGCCAAACTAAACCGTAAAGGATAATGGCGGC‑CATAAAAAATAAAAACACGCCGATAAACGCctgctg                                                                                                                        <  8:161969/139‑1 (MQ=255)
                   aaTGTCCGTAATGTGGCTTATAGGGTTAATCCACGTCATATTTTGCAGCCAAATTGGCATGTTTTCCACCGGCGAAACTTAACCGTAAAGTAGATTGGCTGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCtgtt                                                                                                                      <  8:310400/140‑1 (MQ=255)
                      ctCCGAAACGCGGCCAATTGGTTTAAGCCCAGACAGATTTTGCACCCACATGTGGAGTTTTTCCCCCGGCTAAACGAACCCGGAAAGGACAATGGCGGG‑AATCATAAACAAAAACACGCCGATAAACGCCTGCtgttgtg                                                                                                                   <  3:481537/139‑1 (MQ=255)
                       tCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCtgttgtgt                                                                                                                  >  7:480322/1‑140 (MQ=255)
                       tCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCtgttgtgt                                                                                                                  >  8:360057/1‑140 (MQ=255)
                         cGTAAAGTGGCGAATAGGTTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCAGAATGACAACCACGCCGATAAACGCCTGCtgttgtgg                                                                                                                  >  5:667614/1‑137 (MQ=255)
                           tAAAGTGGCTAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGAATGTTTTCCACCGGCTAAACGTAACCGTAAAGGAGAATGGCGGG‑CATCAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGaa                                                                                                              <  4:504192/140‑1 (MQ=255)
                           tAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGaa                                                                                                              >  1:21997/1‑140 (MQ=255)
                           tAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGaa                                                                                                              >  1:183731/1‑140 (MQ=255)
                           aaatgTGGCCAATAGGGTTAATCCACGTCAGATTTTGCAGCCAAATTGGCTTGTTTTCCCCCGGCGAAACGAACCCGGAAAGGAGAATGGCGGG‑CATCATAAACACAAACACGCCGATAAACGCCTGCTGTTGTGTTGaa                                                                                                              <  2:435707/136‑1 (MQ=255)
                           aaaaGTGGCTAATAGGTTTAATCCACGTCATTTTTTGCAGCCACATTGGAATTTTTTCAACCGGCGAAACGTAACCTGAAAGGTGAAGGGCGGG‑CAACATAACGACAAACACGCCGATAAACGCCTGCtgttgtgttg                                                                                                                <  6:713764/137‑1 (MQ=255)
                           aaaaGGGGCGAATCTGGTTACTCCACGTCAGAGTTTGCCCCCACTTTGGCATGTTTTCCCCGGGCCAACCGTAACCGTAAAGGAGAAGGGCGGC‑CTGCATCAACACACCCACGCCGATAAACGCCTGCTGTTGTGTTGaa                                                                                                              <  8:95776/139‑1 (MQ=255)
                             aaGGGGCTAATAGGGTTAATCCACGTCAGATTTTGCAGCAACATTTGCATGTTTTCCACCGGCGAAACGTAACCGCAAAGGATATTGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACa                                                                                                            <  1:929768/140‑1 (MQ=255)
                              aGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCATGCTGTGGTGTTGAAc                                                                                                             >  5:771717/1‑138 (MQ=255)
                                  gCGAATAGGGTTAATCCACGTCAGATTTTGCAGACACATTGGCATGTTTTCAACCTGCGAAACGTAACCGGAAAGGAGAATGTCGGG‑CATCATAAAGACAAACACGCAGATAAACGCATGCTGTTGTGTTGAACAGAGtt                                                                                                       >  8:315530/1‑139 (MQ=255)
                                      attgggTAAATCCACGTCTTTTTTTGCAGCCAAATTTGGATGTTTTACACGGGCTAAACGTAACCGTATTTTATAATGGCGGG‑CATAATAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGtgatga                                                                                                   <  4:626828/137‑1 (MQ=255)
                                          ggTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATc                                                                                               >  1:106961/1‑140 (MQ=255)
                                          ggTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATc                                                                                               >  7:799644/1‑140 (MQ=255)
                                            ttATTCCACGTCAGATTGTGCAGCTACATTCGCATGATTTCCACCGGCTAAAGGTAACTGTAAAGTAGAAGGGCGGT‑CATCAAAACGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCaa                                                                                             <  4:555639/140‑1 (MQ=255)
                                            ttAATCCAGGTATGATTTGGCAGCCACTTTGGCTTGTTTCCCACCGGCCAAACGAACCCGGAATGGAGATTGGCGGG‑CATAATAAAGAAAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCaa                                                                                             <  8:627852/140‑1 (MQ=255)
                                            ttAATCCACGTCATATTTTGCAGCCACATTGGCATTTTTTCCACCGGCGAAACTTACCCGTAAAGGATAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATc                                                                                               <  6:477530/138‑1 (MQ=255)
                                            taaaTCAACGTCTTATTTTGCCCCCACATGGGATTTTTTTCCACCGGCTAAACGAAACGTTAAAGTATATTGGCGGC‑CATCATAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATc                                                                                               <  6:267126/136‑1 (MQ=255)
                                             tAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAAc                                                                                            >  1:343870/1‑140 (MQ=255)
                                               aTCCACGTCAGATTTTGCAGCCACATTTGCATGATTTCCACCGGCGAAACGTCACCGGAAAGGAGAATGGCGTG‑CATCATAAAGACAAACACGCCGATAAACTCCTTCTGTTGTGTTCAACAGAGTGATGAAATCAACAg                                                                                          >  1:236408/1‑140 (MQ=255)
                                                 ccACGTCAGATTTTGCAGCCACATTGGCATTTTTACCACCGGCCAACCTTACCCGTAACGGAG‑ACGGCGGGCCATAAAAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGAc                                                                                        <  3:516023/140‑1 (MQ=255)
                                                   aCGTCAGATTTTCCAGCCACATTGCCATTTTTTCCACCGGCGAAACGAAACCGTAAAGGCGAATGGCGGG‑CATCTTAAAGACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGAc                                                                                        <  6:512599/138‑1 (MQ=255)
                                                    cGTCAGATTTTGCAGCCACTTTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGa                                                                                     >  8:868110/1‑140 (MQ=255)
                                                    cGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGATAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAAAGCCTGCTGAAGTGATGAACAGACTGATGAACTCAAcagacaga                                                                                     >  8:117436/1‑140 (MQ=255)
                                                    cGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGa                                                                                     >  8:564718/1‑140 (MQ=255)
                                                    cGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGa                                                                                     >  3:150830/1‑140 (MQ=255)
                                                    cGTCAGATTTTGCAGCCACATAGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑TATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGa                                                                                     >  1:496466/1‑140 (MQ=255)
                                                     gTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGaa                                                                                    >  3:461682/1‑140 (MQ=255)
                                                     gTCAGATTTTGCAGCCAAATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATCGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTTTGTTGAACACAGTCATGAACTCAACAGACCTaa                                                                                    >  3:613989/1‑140 (MQ=255)
                                                      tCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGa                                                                                     >  5:477530/1‑138 (MQ=255)
                                                      aaaGATTATGCAGCCACATTCGCATGATTTCCACAGGCTAAACGTAACCGGAAATGAGAATCTCGGG‑CATCATAAAGACAAACCCGCCGATCACAGCCTGCTGTAGGGTTGAACATAGTGATGAAATCAACAGACCGa                                                                                     >  6:722427/3‑138 (MQ=255)
                                                       cAGATTTTGCATCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAAAACGCCGATAAACGCCAGCTCTTGTGTTGAACAGACTGATGCAATCAACAGACCGaa                                                                                    >  6:904872/1‑138 (MQ=255)
                                                          aTTTTGCAGCCACATTCGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAAAGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCATTTGTGTTGAACAGAGTGATCAAATCAACAGACCGAATcccc                                                                               >  7:407935/1‑139 (MQ=255)
                                                           ttttGCAGCCACATTGTCATGTTCCCCCCCTGCGAACCTTAACCGGAATGTTCATTGGCGGG‑ACTAAAAACGAAAAACACGCCGATACACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATccc                                                                                <  5:236038/138‑1 (MQ=255)
                                                           ttttGCAGCCACATTGGCATTTTTTCCACCGGCTAAACTTAACCGTAAAGGAGATTGGCGGG‑CTTCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCccac                                                                              <  1:216055/140‑1 (MQ=255)
                                                           ttttGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGTAGAATGGCGGG‑CATCAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCccac                                                                              <  8:492979/140‑1 (MQ=255)
                                                           ttttGCAGCCAAATTGGCATGTTTTCCACCGGCGAACCGTACCCTTAAAGGATAATGGCGGG‑CAGAATAAATACAAACACGCCGATAACCGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCccac                                                                              <  8:276620/140‑1 (MQ=255)
                                                           ttttGCACCCACATTGGCATGTTTTCCACCGCCGAAACGTAACCGCAAAGGAGATTGGCGGG‑CATAATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCccac                                                                              <  7:733178/140‑1 (MQ=255)
                                                           ttttCCAGCCACATTGGATTTTTTCCCCCCGGCCAACCGTAACCGTAATGGTTATTGGCGGG‑CATAAAAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATccc                                                                                <  5:706884/138‑1 (MQ=255)
                                                                   ccACATTGGCTTGTTTTCCACCGTCTAAACGTAACCGGAAAGGATATTGGCGGG‑CATCATAAACACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATa                                                                      <  1:359908/140‑1 (MQ=255)
                                                                   ccACATTGGCATGTTTTCCACCGGCGAAACGAAACCGTAAAGGAGAATGGCGGG‑CATCATAAACACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATa                                                                      <  1:498877/140‑1 (MQ=255)
                                                                   ccACATTGGCATGTTTGCCCCCGGCGAAACTTCACGGGAAAGGAGATGGGCGGG‑AAGCATAAATAAAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATa                                                                      <  2:107088/140‑1 (MQ=255)
                                                                   ccAAATTGGCTTGTTTTCCACGGGCTAAACGTAACCGGAAAGTTGATTGGGGGG‑AATAATAACCACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATa                                                                      <  3:889780/140‑1 (MQ=255)
                                                                    cacaTTGGCATGTTTTCCACCGTCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATaa                                                                     >  4:727466/1‑140 (MQ=255)
                                                                     aatttGGCATGTTTTCCCCCGGGCAAACGAAACCGTAAAGGAGTATGGGGGG‑CAGCAAAATGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATaaa                                                                    <  4:103232/137‑1 (MQ=255)
                                                                             ttGTTTTCCACCGGCTAAACTAAACGTGAAAGTATATTGGGGGG‑CATCAAAAATAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTaa                                                              <  5:478298/137‑1 (MQ=255)
                                                                               gTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAacca                                                          >  2:730596/1‑140 (MQ=255)
                                                                                ttttCCCCGGGCGAAACTTAACCGTAACGGATAATGGCGGC‑CATCATAAACAAAACCACGCCGATAAACGCCTGCTGTTGTGGTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAacc                                                           <  6:612308/138‑1 (MQ=255)
                                                                                ttttCCCCCGCCTAAACGAAACCGGAAAGGAGATTGGCGGG‑CATCAAAATTACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAacc                                                           <  6:755084/138‑1 (MQ=255)
                                                                                ttttCCACCGGCTAAACGTAACCGTAAAGGTGAATGGCGGG‑CATAAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAaccac                                                         <  1:690729/140‑1 (MQ=255)
                                                                                ttttCCACCGGCCAACGTTAACGGCAAAGTAGATTGGGGGG‑CATATTAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAaccac                                                         <  7:396411/140‑1 (MQ=255)
                                                                                 tttCCACCGGCGAAACGTACCCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGCCCGCATCCCACCAGTGCTAAACCGTAAaccacc                                                        >  7:267223/1‑140 (MQ=255)
                                                                                 tttCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTCAACAGAGTGATGAAATCACCAGACCGAATCCCACCAGTGATAAACCGTAAaccacc                                                        >  3:112904/1‑140 (MQ=255)
                                                                                     cccgggCGAAACGTAACCGTAATGTAGATTGGGGGG‑AATAATAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCg                                                    <  7:565272/136‑1 (MQ=255)
                                                                                     ccccGGCGACACGTACCGGTACATGTGAATGGCGGG‑AATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCg                                                    <  1:201637/138‑1 (MQ=255)
                                                                                     cACGGGCTAAACGTAACCGGAAAGGATAATGGCGGC‑CATCAAAAAGACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCAt                                                      <  6:583484/138‑1 (MQ=255)
                                                                                     cACCGGCGAAACGTAACCTGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAACCGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGAACGAATCCCCCCAGTGATAAACCGAAAACAACCATcc                                                    >  3:857286/1‑139 (MQ=255)
                                                                                     cACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGACCAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCg                                                    >  8:115853/1‑140 (MQ=255)
                                                                                       ccGGCTAAACGTAACCGCAATGTATATGGGCGGG‑CATCAAAAACACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTa                                                  <  2:340010/140‑1 (MQ=255)
                                                                                       ccGGCTAAACGTAACCGCAAAGGATAATGGCGGG‑CATAATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTa                                                  <  3:924021/140‑1 (MQ=255)
                                                                                          gCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCCCCATCGTAAAg                                               >  7:139257/1‑140 (MQ=255)
                                                                                          gCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCAGCGTAAAg                                               >  4:726654/1‑140 (MQ=255)
                                                                                            gAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAg                                               >  6:103943/1‑138 (MQ=255)
                                                                                               aCGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGaa                                          >  3:809204/1‑140 (MQ=255)
                                                                                                          aaGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAg                                 >  5:54637/1‑138 (MQ=255)
                                                                                                                aaTGGCGGG‑CATCATAAAGACAAACACGCCGATAACCGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCgg                         >  3:813372/1‑140 (MQ=255)
                                                                                                                    gCGGG‑CAACATAAAGACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGaa                     <  7:821644/140‑1 (MQ=255)
                                                                                                                       gg‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAACCCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAggg                  >  2:945406/1‑140 (MQ=255)
                                                                                                                          catcatAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAggg                  >  5:794481/1‑138 (MQ=255)
                                                                                                                          aatcatAAATACAAACACGCCGATAAACGCCTGCTGTTGTGTGGAACAGAGTGATGATATCAACAGAGCGAATCCCTCCAATGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAggg                  >  6:662190/2‑138 (MQ=255)
                                                                                                                               tAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAAttt             >  5:486485/1‑138 (MQ=255)
                                                                                                                               tAAAGACAAAAACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAAttt             >  6:30555/1‑138 (MQ=255)
                                                                                                                                 caGACAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATa         <  4:29643/139‑1 (MQ=255)
                                                                                                                                 accaCAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATa         <  8:807910/137‑1 (MQ=255)
                                                                                                                                 aatccAACCACGCCGATAAACGCCTGCTGTTGTGATTAACAGAGTGATGAAAGCACCAGAACGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAACGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATa         <  7:410365/136‑1 (MQ=255)
                                                                                                                                 aatccAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATa         <  4:867433/136‑1 (MQ=255)
                                                                                                                                 aataCAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATAAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATa         <  8:634454/137‑1 (MQ=255)
                                                                                                                                 aacaCAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATa         <  8:672388/137‑1 (MQ=255)
                                                                                                                                 aaGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGa           <  6:334393/138‑1 (MQ=255)
                                                                                                                                     cAAACACGCCGATAAACGCCTGCTTTTTTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCATTGATAAACCGTAAACCACCATCGTAAATTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACg       <  6:9629/138‑1 (MQ=255)
                                                                                                                                       aaCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGCGTGATGAAATCAACCGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACGCCCa   >  8:307766/1‑140 (MQ=255)
                                                                                                                                       aaCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACGcc     <  6:632661/138‑1 (MQ=255)
                                                                                                                                       aaCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACAGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACGCCCa   >  8:572089/1‑140 (MQ=255)
                                                                                                                                        acacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACGCCCAg  >  1:569964/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCACATTGGCATGTTTTCCACCGGCGAAACGTAACCGGAAAGGAGAATGGCGGG‑CATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGTGATAAACCGTAAACCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGAATTTGATACGCCCAG  >  NZ_CP022686/4331805‑4332079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A