Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,032,292 A→C 16.0% E106D (GAA→GAC)  glnK → P‑II family nitrogen regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,032,2920AC16.0% 170.8 / 2.1 115E106D (GAA→GAC) glnKP‑II family nitrogen regulator
Reads supporting (aligned to +/- strand):  ref base A (58/32);  new base C (2/20);  total (61/54)
Fisher's exact test for biased strand distribution p-value = 2.11e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGAT  >  NZ_CP022686/4032163‑4032426
                                                                                                                                   |                                                                                                                                      
ggCGATTGCTGATGACCAACTCGATGAAGTGAGCGATATCGTCCGCAATGCGGCTTACACCGTAAAAATT‑GGCGACGGAAAAATCTTCGTCGCTTAATTGCACCGCGTCATTCG‑TATTCGTCCCGGCGACGCCGACGAAg                                                                                                                              <  6:872584/140‑1 (MQ=255)
ggCGATTGCGGATGACCAACCCGATGAAGTGAGCGATATCGTCACCAAAGCGGCTCACCCCGTAAAAATG‑GGCGACGGAAAAATCTTCGGCGCTGAATTGCACCGCGTCATTCG‑TATTCGACCCGGCGAAGCCGACGAAg                                                                                                                              <  2:865675/140‑1 (MQ=255)
ggCGAGTGCTGATGACCACCGCGATGAAGTTATCTATATCGTCACAAAAGCGTCTTACCCGTGAAAAATT‑GGCGACGGCAAAATCTTCTCCCCTTAATTCCACCGCGTCATTCG‑TATCCGAACCGGCGAAGCCGACGAAg                                                                                                                              <  8:1077543/140‑1 (MQ=255)
  gtaTTGCGGATGACAAACGCGATTAAGTGATCGATCTCGTCACCAAAGCGGCTTACCCCGGAAAAATT‑GCCGCCTGAAAAATCTTCGGCGCTTAATTGCACCGCGTCATTCG‑TTTTCGTCCCGGCGAAGCCGACGAAgcg                                                                                                                            <  6:1108530/138‑1 (MQ=255)
   gATTGCTGATGCCCAACTCGATGAAGTGATCGATATCGTCCGCAACGCGGCTTACACCGTAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCACCGCGTAATTCG‑TATTCGTACCGGCGAAGCCGACGAAgcgg                                                                                                                           <  2:537353/140‑1 (MQ=255)
      tGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGgcgc                                                                                                                        >  1:147132/1‑140 (MQ=255)
        cTGATGACCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGgcgc                                                                                                                        >  4:1002952/1‑138 (MQ=255)
          tatcaCCAACTCTTTTAAGGGAGCTATATCGAAAGCAAGGCGGCTT‑CCCCGGTAAAATTGGGCGCGGGAAATATTTTCGTCGCTGTATTCCACCGCGGCATTCT‑TATACGTCCCGGCGAAGCCGACGAAGCGGCGCTGTa                                                                                                                    <  6:160371/136‑1 (MQ=255)
          gatgaCCAACTCGATTAAGTAATCGATATCGTCAGCAAAGCGGCTTACACCGCAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTg                                                                                                                      <  3:839462/138‑1 (MQ=255)
           atgaCCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTaa                                                                                                                   >  5:511473/1‑140 (MQ=255)
           atgaCCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTaa                                                                                                                   >  7:936332/1‑140 (MQ=255)
           atgaCCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGAGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTaa                                                                                                                   >  8:512273/1‑140 (MQ=255)
             gaCCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTaa                                                                                                                   >  3:630190/1‑138 (MQ=255)
              aCCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCTTACCGGCGAAGCCGACGAAGCGGCGCTGTAAtct                                                                                                                >  6:179936/1‑140 (MQ=255)
               ccACCTCGTTCATGTGATCTTTATCGCCACCAACGCGGCTAACACCGGAAAATTT‑GGCCACGGCAAAATCTTCGTCGCTTAATTCCACCGCGTAATGCT‑TATTCGTACCGGCGACGCCGACGAAGCGGCGCTGTAAtctc                                                                                                               <  2:157433/140‑1 (MQ=255)
                cAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCGGACGAACCGGCGCTGTAAtct                                                                                                                >  4:697266/1‑138 (MQ=255)
                   cTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGc                                                                                                           >  1:1051481/1‑140 (MQ=255)
                    tCGATTAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGTAAAAATT‑GGCGACGCCAAAATCTTCGGCTCTTAATTCCAACGCGTCATTCG‑TTTTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGca                                                                                                          <  5:652261/140‑1 (MQ=255)
                          aaGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcacaca                                                                                                      >  3:237671/1‑138 (MQ=255)
                           aGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCa                                                                                                   >  6:69475/1‑140 (MQ=255)
                            gTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGCCGAAGCGGCGCTGTAATCTCTGGCACACAGCaa                                                                                                  >  2:684135/1‑140 (MQ=255)
                             tGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCACCGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAAc                                                                                                 >  6:862699/1‑140 (MQ=255)
                              gATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACa                                                                                                >  5:471044/1‑140 (MQ=255)
                                tCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGAGTCATTCG‑TATTCGTACATGCGAAGCCGACGAAGCGTCGCTGTAATCTCTGGCACACAGCCACTgg                                                                                              >  6:274279/1‑140 (MQ=255)
                                 cGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCCACGCGTCATTCG‑TATTCGTACCGGCGCAGCCGCCGCAGCGGCGGGGTAATCTCTGGCACACAGCAACAGGa                                                                                             >  7:1003718/1‑140 (MQ=255)
                                   atatCTTCAGCAATGCGGGTACCAACTTAAAAATT‑GGCGACGGCAAATTCTACGGCCTTGATTTGAAAAGCGTCATTCG‑TATTTGTACTGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGTCACACAGCAACATGAAc                                                                                           <  6:193082/140‑1 (MQ=255)
                                        gTCAGCAAAGCGGCTAACACCGGAAAAATT‑GGCGAAGGCAAAATCTTCGTAGCTGAATTGCAACGCGTCATTCT‑TATTCTTACCGGCGACGCCGACTAAGCGTCGCTGGAATATCTGACACAAAGCCACAGTAACGaa                                                                                        >  4:882307/1‑138 (MQ=255)
                                         tCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGaaaaa                                                                                     >  1:156596/1‑140 (MQ=255)
                                         tCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGaaaaa                                                                                     >  5:780014/1‑140 (MQ=255)
                                         tCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATAGAAACGCGTCATTCG‑TATTCGTACCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAAAGaaaaa                                                                                     >  5:101613/1‑140 (MQ=255)
                                           aGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAAtt                                                                                   >  2:3739/1‑139 (MQ=255)
                                           aGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATg                                                                                   >  1:742149/1‑140 (MQ=255)
                                                aGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGAt                                                                              >  2:925358/1‑140 (MQ=255)
                                                aGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGAt                                                                              >  6:845868/1‑140 (MQ=255)
                                                  cGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAATCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAAAGAAGATAg                                                                            >  5:274927/1‑140 (MQ=255)
                                                    tcTTAGACTGGAAAATTT‑GCCGTCGGAAATTTTTTCGGAGCTGAATTGCAAGGGGTCATTCG‑TATTGGTTGCGGCTAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCa                                                                          <  6:167573/139‑1 (MQ=255)
                                                    gtttACACCGGAAAAATT‑GGCGACGCCAAAATCTGCGGCGCTTAATTGCACCGCGTCATTCT‑TATGCGTCCCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCa                                                                          <  5:79041/138‑1 (MQ=255)
                                                    gtttACACCGAATATTTT‑GGCGCCGTCAAATTTTGCTGCGCTTAAGTGCAACGCGT‑ATTGGTTATTCGTACGGGCGACGCGGACGATGCTGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAAGGAAGATAGCa                                                                          <  6:32339/138‑1 (MQ=255)
                                                    gattACACCGTAAAAATT‑GGCGAGGGAAAATTCTTGGAGGCTTAATTGAAACGCGTAATTCT‑TTTTCTTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGTAACGAAAAATGAAGATAg                                                                            <  3:598445/136‑1 (MQ=255)
                                                    gCTTACACCGCATAAATT‑GGCGCCGGCAAAAACTTCGTCGCTTATTGGCACCGCGCCATGCG‑TATTCGGACAGGGGACGCGGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCa                                                                          <  8:350155/140‑1 (MQ=255)
                                                     cTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGCTAGCaa                                                                         >  6:375391/1‑140 (MQ=255)
                                                         ccccGAAAAATTT‑GGCGCAGCAAAAATCTTCGTCGGTTATTTGCCAGGCTTAATTCG‑TTTTGGTCCCGGCGATGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACATGAACGAAAAATGAAGATAGCAACGAt                                                                     <  1:52698/138‑1 (MQ=255)
                                                         cacCGGAAAAATT‑GGCGCCGGCAAAATCTTCGTCGCTGAATTCCACCGGTTCATTCG‑TATTCGTCCGGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACg                                                                       <  4:289887/138‑1 (MQ=255)
                                                             ttaaaaaTT‑GGCGCCGTAAAATTCTTCGGCGCTTATTTGCAACGCGTAATTGT‑TTTTCGTCCGGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATaa                                                                   <  3:732539/136‑1 (MQ=255)
                                                              gAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGTAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATaaaaa                                                                >  2:574961/1‑140 (MQ=255)
                                                              gAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGCCGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATaaaaa                                                                >  8:492994/1‑140 (MQ=255)
                                                              gAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATaaaaa                                                                >  2:451103/1‑140 (MQ=255)
                                                              gAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATaaaaa                                                                >  7:198935/1‑140 (MQ=255)
                                                               aaaaaTT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCTACGAAGCGGCGCTGTAATCTCTGTCACACAGCAACAGGAACGAAAATTGAACATAGCAACTATaaaatc                                                               >  5:485129/1‑138 (MQ=255)
                                                                aaaaTT‑GGCGCCGGCAAAATCTTCGTCGCTTAATTGCAACGCGTCATTCG‑TATTCGAACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACt                                                              <  8:763512/140‑1 (MQ=255)
                                                                aaaaTT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATaaaaa                                                                >  3:793683/1‑138 (MQ=255)
                                                                aaaaTG‑GGCGACGCAAAAATCTTCGGCGCTTAAGTCCAACGCGTCATTCT‑TATTCGACCGGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACt                                                              <  8:16198/140‑1 (MQ=255)
                                                                   ttt‑GGCTACGTCAAAATATTCGTGGCTTAATTCCACCGCGTCATTCG‑TATGCGTACCGGCCACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTg                                                             <  3:414610/137‑1 (MQ=255)
                                                                   aTT‑CGCGACGGCAAAATCTCGGTGGCTTAATTGCAACGCGTCTTTCG‑TATTCGTCCCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTg                                                             <  3:1015757/138‑1 (MQ=255)
                                                                      gggCTACGGAAAAATCTTCGTGTCTGAATTCCACCGCGTCATTCG‑TATTCGTCCCGGCTAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTggg                                                           <  4:1139083/137‑1 (MQ=255)
                                                                       ggCGACGGCAAAATCTTCGACGCGGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCACCAGGAACGAAAAATGAAGATAGCAACGATACAAACTtgggtg                                                        >  6:249627/1‑137 (MQ=255)
                                                                            ctccAAAATCTTCGTCGTTTAATTCATACGCTTAATTTT‑TATTCTTACGGGTCAAGCGTAAGAATCGGTGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTc                                                   <  8:518568/137‑1 (MQ=255)
                                                                             ggCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGCAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCa                                                  >  7:237834/1‑140 (MQ=255)
                                                                                     tttCGTCGCTGAATTGCAACGCGTATTTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGAt                                          <  6:856116/139‑1 (MQ=255)
                                                                                     cTTCGTCGCTTAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCg                                            <  4:120963/138‑1 (MQ=255)
                                                                                      ttCGTCGGTTATTTGAAACGGGTTATTCG‑TATTCTTACCGGCCAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGa                                           <  4:578760/138‑1 (MQ=255)
                                                                                       tcgtcgCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTTGCGCtgc                                        >  8:295050/1‑140 (MQ=255)
                                                                                          cccatGAAGTCCAACGGGTAACTCC‑TATCCGCCCCGGCCACCCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGAtgctgc                                     <  2:352554/136‑1 (MQ=255)
                                                                                             gttaaTTGCAACGCGTCATGCG‑TATTCGTACCGGCTAAGCCGCCGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCgg                                  <  2:915220/137‑1 (MQ=255)
                                                                                             cctaaTTGCAAGGCGTCTTTGG‑AATCCTACCCGGAGACGCCCACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGTAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCgg                                  <  1:148472/137‑1 (MQ=255)
                                                                                              tGAATTCCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCg                                   <  3:941166/138‑1 (MQ=255)
                                                                                               tattgtcAAGGCGGCATTCG‑TTTTCGCCCCGGCGACGCCGCCGAAGCGGCGCTGTAATCTCTGGCACACAGACACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGa                                <  7:431730/134‑1 (MQ=255)
                                                                                               taaTTTCAAAGCGGAATTCT‑TATACGAACCGGCTCACCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGa                                <  5:593217/139‑1 (MQ=255)
                                                                                               taaTTGCACCGCGTCATTCG‑TATTCGTACCGCCTACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGa                                <  2:818553/139‑1 (MQ=255)
                                                                                               taaTTGCAACGCGTCATTCG‑TATACGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCgg                                  <  4:1051245/137‑1 (MQ=255)
                                                                                               taaTTGCAACGCGTATTTCT‑TATGCTTCCCGGCGCCCCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGa                                <  2:153846/139‑1 (MQ=255)
                                                                                               gAATTGCAACGCGTCATTCG‑TATTCGCACCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGa                                <  1:828987/140‑1 (MQ=255)
                                                                                               caaTTGCACCGCGTCAGTCT‑TATCGGGACCTGCGATCCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGACGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGa                                <  1:146809/139‑1 (MQ=255)
                                                                                                     caccgcgTCTTTCT‑TATTCGTAACGGCTAAGCCTTCGAAGCGGCGCTGTAATCTCTGGCACACATCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTa                          <  2:572004/137‑1 (MQ=255)
                                                                                                     cAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTgg                            <  4:772038/138‑1 (MQ=255)
                                                                                                       aCGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGAGAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTa                          >  4:523738/1‑138 (MQ=255)
                                                                                                          ggtacttct‑tattcgtcCCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGc                     <  2:1021681/135‑1 (MQ=255)
                                                                                                          cgTTATTCT‑AATTCGTACCTCCTAACCCTACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGc                     <  7:449263/140‑1 (MQ=255)
                                                                                                          cgTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGc                     >  1:818553/1‑140 (MQ=255)
                                                                                                          cgTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGc                     >  1:144248/1‑140 (MQ=255)
                                                                                                          cgTCAGTCT‑TTTTCGTCCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGc                     <  1:815400/140‑1 (MQ=255)
                                                                                                          agaatttcc‑taatcgtcCCGCCTAACCCGACGAAGCTTCGGTGTAATCTCTGGCACACAGCTACTTTAACGAAAAATGAAGATAGCAACGATAAAAAATGGGCTTACTTCACTGTCGATGCTGCCGGGACTGGTAATg                       <  4:544052/133‑1 (MQ=255)
                                                                                                            tCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCACCGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGc                     >  3:324027/1‑138 (MQ=255)
                                                                                                               ttct‑tattcttcCCGGCTAAGCCGACGAATCGGCGCTTTAATCTCTGGCACACAGCAACACGAACGAAAAATGAAGATATCAACTATAAAAACTGGGCTTACTTCACTGTCGATGCTGCCGGGACTGGTAATGGCTGCAc                <  8:395941/140‑1 (MQ=255)
                                                                                                                    tattcgtaCCGGCGATGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAATAATGAAGATAGCAACGATAAAATCTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGc            >  1:1113580/1‑140 (MQ=255)
                                                                                                                     attcgtaCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGATGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCg           >  6:721747/1‑140 (MQ=255)
                                                                                                                     atgcttaCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCg           <  5:1104336/135‑1 (MQ=255)
                                                                                                                      ttcgtaCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCgg          >  1:848217/1‑140 (MQ=255)
                                                                                                                        cttaCCGGCTACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTg        <  2:44294/138‑1 (MQ=255)
                                                                                                                        cgtaCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTg        >  7:961421/1‑140 (MQ=255)
                                                                                                                        cgtaCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGAACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGATTACTTCACTGGCGATGCTGCCGGGCCTGGTAATGGCTGCACCTGCgg          >  3:918538/1‑138 (MQ=255)
                                                                                                                         gtcccGGCTAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGt         <  3:506575/135‑1 (MQ=255)
                                                                                                                          taCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGAt                                                                     <  2:823939/77‑1 (MQ=255)
                                                                                                                          taCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGAt                                                                     >  1:823939/1‑77 (MQ=255)
                                                                                                                           aCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTTGAACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGTCTTACTTCACTGGCGATGCTGCCGTGAGTGGTAATGGCTGCAACTGCGGTGTcc     >  7:807775/1‑140 (MQ=255)
                                                                                                                           aCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAATACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGcc     >  8:94607/1‑140 (MQ=255)
                                                                                                                           aCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGcc     >  2:22907/1‑140 (MQ=255)
                                                                                                                           aCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGcc     >  2:1449/1‑140 (MQ=255)
                                                                                                                              ggCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGAt  <  1:97662/140‑1 (MQ=255)
                                                                                                                                cGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGAt  >  3:739086/1‑138 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGCAAAGCGGCTTACACCGGAAAAATT‑GGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCG‑TATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCAACGATAAAAACTGGGCTTACTTCACTGGCGATGCTGCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGAT  >  NZ_CP022686/4032163‑4032426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A