Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F2 I0 R1
|
117 |
5184.3 |
6857682 |
98.3% |
6741101 |
135.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NZ_CP022686 |
1,547,113 |
G→C |
20.5% |
intergenic (+25/‑9) |
CIW80_RS07710 → / → CIW80_RS07715 |
tRNA‑Met/tRNA‑Met |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP022686 | 1,547,113 | 0 | G | C | 20.5%
| 61.7
/ 4.0
| 42 | intergenic (+25/‑9) | CIW80_RS07710/CIW80_RS07715 | tRNA‑Met/tRNA‑Met |
| Reads supporting (aligned to +/- strand): ref base G (24/9); new base C (4/4); total (28/14) |
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT > NZ_CP022686/1546974‑1547239
|
aaCTAATTAAAATTTGTAGAAGTATAGGATTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAg < 5:281909/140‑1 (MQ=17)
ccAATTAAAAATTGATGAAGAAACGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTa < 3:200099/140‑1 (MQ=255)
tACGGTTACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATATCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATGTGAGGAAATAAAGCACTAAGGTGACTCAGGGTTGAGCAGACTGGtt > 4:550367/1‑139 (MQ=14)
cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAAATTAAAGATATAAAACACTACGCTGCATCAGGGTGGAGCAGCGTgggg > 7:92639/1‑136 (MQ=11)
cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTAGATGAAATAAAGCAGTACGCTGCCTCGGGGTGGcgcagctttgtt > 7:92635/1‑132 (MQ=11)
tGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAATTTTGATGATATAAAGCAGTACGGTGAAGGGGGGTGGAGCAGCATGGTAgttag > 3:533314/1‑136 (MQ=11)
aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGCTGACGCTGGGTGGAGCAGCCTGGTATCtagac > 3:570997/1‑136 (MQ=255)
ctcgtggtggtGAAGCGTGGTCGCTCGTCTGTCTCATATCCCGAATGGCGTCGGTGCCTATCCGGCCCGCTCAACCAATTCAAATTTTTTGAATTAACCCCGTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg < 4:128117/135‑1 (MQ=255)
cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAATTTTGATGTAATAAAGCAGTACGGTGACGCGGGGTGGAGCTGCATGGTAGCtcgagggg > 3:270237/1‑135 (MQ=11)
gggTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTATTTTTGATGATATAAAGCACTAAGGTGCCCCGGGGTGGAGCAGGATTGTAGCTAGTCggg > 7:482574/1‑138 (MQ=11)
gggTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAACCAGTACGGTGCCGCGGGGTGGAGCAGCCTGGGAGCTCGTCggg > 2:33320/1‑138 (MQ=255)
gggAGAAGCATGGTCGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGGGCCAATCCGGCCCCCGCAACCAATCAAACATTGCAGAAATAAAGCCGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGGCGGGCTCAt < 3:244341/139‑1 (MQ=14)
gAGCAGCATGGTAGCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAAATTTGATGATATAAAGCAGTACGGTGACGCGGGGTGGAGCTGCCTGGTAGCTCGTCGGGCTCaa > 2:658705/1‑137 (MQ=255)
gcctGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGGCGCTTCAAATCCGGCCCCCGCAACCAATCAAATTTTGATGAAATCAAGCAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGa < 4:273194/137‑1 (MQ=11)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTTGATGAAATAAAGCACTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCACATAACCCGaa > 3:818598/1‑140 (MQ=255)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag < 5:103238/107‑1 (MQ=38)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag > 6:103238/1‑107 (MQ=38)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAACAGTACGCTGACTCGGGGTTGAGCAGAGTGGTAGGTATTCGTGCTAATAACCAGaa > 4:529603/1‑140 (MQ=11)
aGCTAGGCGGTCTCATAACCCGAAGGTCGTCGGTTCTAATCCGTCCCCCGCCACCAATAAAAAATTGAAGAAATTAAGCACTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc < 1:19350/138‑1 (MQ=11)
gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATTTTTGAGCAAAAAAAGCGGTACGGTGCGGGGGGGTGGCGCAGGCTGGTAGATAGACGGGCGGATCACCGGAATgtcg > 1:717008/1‑138 (MQ=2)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCgggg < 5:190350/92‑1 (MQ=37)
gggCTCATAACCCGAAGGTCGTCGGTTCATATCCGGCCCCCGCAACCAATAAAATTTAGTTGAAAAAAACCAGTACGGTGACGAGGGGTGGAGCAGCGTTGGAGATCTTCGGGCGATTAACCCTAATCTCATCGCTTc > 2:177124/1‑138 (MQ=2)
ggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGAGGAAATAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGATCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCa > 7:160740/1‑138 (MQ=255)
aCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGTAAAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTAGTCGGTCTCATACCCCGAAGGTCGTCGCTTAAAAACCGGc > 1:275788/1‑138 (MQ=11)
aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATATTTGATGAAAGAAAGCAGTACGGTGCCGCGGGGTGGCGCAGCATTGTAGCTCGTCGTGCGAATCACCCGTAGGTCGTCGGTTAGAATACGGCACCCGCa > 6:683735/1‑140 (MQ=11)
tgtgggcgGTTCTAATAATGCCGCCTCAACCAATAAAATATTCATTAAATAACCCAATACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc < 4:267649/134‑1 (MQ=11)
ggccgtcgGTGCTAATCAGCCCCCCTCAACCAATCACATATAGATGTAATATAGCACTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc < 6:606070/137‑1 (MQ=11)
ggTCGTAGGTGCAAATCCTCCCGCCGCAAAGAATAATAATTTTATGAATTATAGGAGTACCGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc < 5:105936/140‑1 (MQ=11)
ggTTCAAATCCGGCCCCCGCAACCATTCAAAATTTGATGAAATAAAGCAGTACGGTGCCGCGGGGTTGAGCAGCCTGGTAGCTCGTCGGGCGCATAACCCGAAGGTCGTCCGTTCAACTACGGCCCCCCCGCCCAAtt > 1:28053/1‑138 (MQ=11)
ggTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGTTGAAATAAAGCAGAACGGTGACGCGGGGTGGAGCATACTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAACTCCGGCCCCCGCTACCAAtt > 1:708532/1‑138 (MQ=11)
aTCCGGCCCCCGCAACCAATGAAAATTTTATGAAATAAAGCAGTACGGTGCCGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCAACTCCGGCACCCGCCACAAATTTATTAGAAt > 3:185117/1‑140 (MQ=14)
gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCACCAATTTATTAGAATGCTGa > 5:301085/1‑140 (MQ=255)
cgccGCAATCAATAAAACTTTTCTGTAAAAAACGACTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGa < 7:372367/136‑1 (MQ=14)
ccccGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg < 7:698733/54‑1 (MQ=255)
ccccGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg > 8:698733/1‑54 (MQ=255)
ccccGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCACCCGCAAACAATTAATTAGAAtgatga > 1:198350/1‑138 (MQ=14)
ccccGCAACCAATAAAAAATTCCTGAAAAAAACCACTACAGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAGCCGGCCCCCGCAACCAATTTATTAGAATGCTGa < 2:193881/138‑1 (MQ=14)
aTCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTAGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgc > 6:71289/1‑140 (MQ=255)
aTCAAAATGTGCTGAATTAATGCAGTACCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgc < 3:457470/140‑1 (MQ=18)
aaaaTTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgct > 8:437326/1‑138 (MQ=255)
atgaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTgg > 2:852625/1‑138 (MQ=255)
gaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGATGACAAACCGCAAAGCTGCTATTGGCt > 8:357185/1‑138 (MQ=255)
gaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCTGTGCATATCCGGCCCCCGCAACCAATTTATTAGAATGATGACAAACCGCAAAGATTCTATAGGCt > 2:486776/1‑138 (MQ=18)
|
AACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT > NZ_CP022686/1546974‑1547239
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
