Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F35 I0 R1 174 9808.2 8488800 98.4% 8352979 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,833,756 T→G 18.8% Q464P (CAG→CCG)  gspD ← type II secretion system secretin GspD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,833,7560TG18.8% 225.1 / 8.6 143Q464P (CAG→CCG) gspDtype II secretion system secretin GspD
Reads supporting (aligned to +/- strand):  ref base T (29/74);  new base G (26/2);  total (67/76)
Fisher's exact test for biased strand distribution p-value = 3.72e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CTGTTATTAGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGATCACCGTGG  >  NZ_CP022686/1833621‑1833894
                                                                                                                                        |                                                                                                                                           
cTGTTATTAGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGt                                                                                                                                           <  2:879139/138‑1 (MQ=255)
cTGTTATTAGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGt                                                                                                                                           <  2:352062/138‑1 (MQ=255)
cTGTTATTAGAGCCAACAGTAGAGCCGGTAAATACCGCAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGCTTATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTAc                                                                                                                                         <  8:506246/140‑1 (MQ=255)
   ttattaGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTAcc                                                                                                                                        <  2:557944/138‑1 (MQ=255)
     attaGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAgcg                                                                                                                                    <  8:889487/140‑1 (MQ=255)
     attaGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAgcg                                                                                                                                    <  3:390979/140‑1 (MQ=255)
          agCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTGCC‑CCCAGGACCCGCCCCATc                                                                                                                               >  8:197780/1‑140 (MQ=255)
          agCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTTACC‑GCCCGTACCAACGcccaa                                                                                                                               >  5:479237/1‑137 (MQ=255)
            ccAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCTGTAACCGCGCCaac                                                                                                                               >  2:213174/1‑136 (MQ=255)
              aaCAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGt                                                                                                                           <  4:1078032/140‑1 (MQ=255)
                cAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGCAGAAGGCTTCCTGGTTGTCCAGCGTGTTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGTAC‑GGCTGTAACAGACCCCTCCAGtgg                                                                                                                         >  3:223760/1‑138 (MQ=255)
                cAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATccccg                                                                                                                           >  1:574461/1‑135 (MQ=255)
                  gTATAGCCGGTTAATACCGGAACGTCCTGGCCAACCATGAAGAAGGCTTCCTGTTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGcc                                                                                                                       <  4:635709/140‑1 (MQ=255)
                        ccGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGGCC‑GCCGGTACCCCCGCCCTCCACTCGCCATTaac                                                                                                                 >  4:322348/1‑140 (MQ=255)
                        ccGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGACCTGGAGTCGTTTTTGTCC‑GCGGGGACCCGCGGCCTACAGTAGCCGTTccc                                                                                                                 >  4:42078/1‑138 (MQ=255)
                         cGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGGTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTcacc                                                                                                                <  4:221995/140‑1 (MQ=255)
                          ggTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTACAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCCGCGCCATCCACTGGCCTTTcac                                                                                                                 >  2:157917/1‑138 (MQ=255)
                           ggtAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GGCTGTACCCGCGCCAAACAGTCGCCGTTcaccac                                                                                                              >  4:428693/3‑140 (MQ=255)
                           gTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGTGAGCACGTTCGAGCTGGCGTCGTTTTTGGAC‑GCCTGTACCCGCGCCCAGCAGGCGCCTGTcccccc                                                                                                              >  6:1041042/1‑138 (MQ=255)
                           gTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCGGGTCCAGCCCCAACCCCTCCCCTTTcacccc                                                                                                              >  4:841237/1‑138 (MQ=255)
                           gTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCGGTACCCGCGCCCACCAGTCGCCGTTtacccc                                                                                                              >  3:34471/1‑138 (MQ=255)
                             tAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCGGGACCAGCGCCATCCCGTCGCCGTTcacccc                                                                                                              >  1:11564/1‑136 (MQ=255)
                              actACCGGAACGGCCTGGCCCACCATGACTAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑cc                                                                                                          <  8:143823/138‑1 (MQ=255)
                                     gAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCCGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGCAC‑GCCTGTACCCGCGCCATCCAGGCGCCGTTCACCCCG‑CCAAccgcc                                                                                                   >  4:810486/1‑140 (MQ=255)
                                     gAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTACC‑GCCGGTACCCGCGACCTCCCGTCGCCGTTAACCCCC‑CCACccgcc                                                                                                   >  8:189503/1‑140 (MQ=255)
                                     gAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGGCCGGCGGGTACC‑GCGCCCTACAGGCGCCGTTCACCCCC‑CCCAccgcc                                                                                                   >  7:763837/1‑140 (MQ=255)
                                     gAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGGCC‑GCCTGTACCAGCGCCAACCACTCGCCGTTCACCCCG‑CCCAccccc                                                                                                   >  4:901041/1‑140 (MQ=255)
                                     gAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCTGTAACAGGGCCCTCCAGTCGCCTTTCACCacc‑cccaccccc                                                                                                   >  4:339666/1‑140 (MQ=255)
                                      aaCGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GACGGGTCCCGCGCCCTACCGTCGCCGTTAAACCCG‑CCCAcccccc                                                                                                  >  5:864880/1‑139 (MQ=255)
                                      aaCGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGTCC‑GCCGGTTCCAGCGCCCGCCCGTCGCCGTTCACCACC‑CCCCccgccc                                                                                                  >  6:43438/1‑139 (MQ=255)
                                       aCGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTACC‑GCCGGTTCCATCGCCATACAGTCGACATTCAACACC‑CCAAccgcc                                                                                                   >  1:391285/1‑138 (MQ=255)
                                       aCGTCCTGGCCCACCATGAAGAAGGCTTCCTGGGTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGGCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAAccgcc                                                                                                   <  2:27859/138‑1 (MQ=255)
                                        cGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGGGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTa                                                                                                <  8:908290/140‑1 (MQ=255)
                                           ccTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTCAC‑GCCAGTACCCGCGCCATCCACTCTCCTTTCACCCCG‑CCAACCCCCGTaacc                                                                                             >  5:836875/1‑137 (MQ=255)
                                           ccTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCTGTAACAGCGCCATCCACGCGGCGTTCACCCCG‑CCAACCCCCGGACCg                                                                                             >  8:205771/1‑140 (MQ=255)
                                           ccTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCGGTAACAGCGCCAACCAGTGGCCCATCACCACC‑CCAAcccccgaaacc                                                                                             >  6:589597/1‑135 (MQ=255)
                                             tGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCg                                                                                             <  2:1045790/138‑1 (MQ=255)
                                             tGGCCACCCATGCAGCAGGCTTCCTGGGTGGCGAGCGTGGTGATTCTCTGCGTGGATAGCCCGTTCGAGCTGGAGTCGTTTTTGCCA‑GCCTGTACCATCGGCATCCTGTCGCCTTTCAACACG‑GCAACCGACGTAACGCt                                                                                           <  6:83484/140‑1 (MQ=255)
                                              ggCACACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGc                                                                                            <  2:345429/138‑1 (MQ=255)
                                                cccACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTAAC‑GCCGGTTCCCGCGCCCTCGCGTCGCCGTTAACACCG‑CCAACCGCCGTAACGCGaaa                                                                                        >  7:889936/1‑140 (MQ=255)
                                                  caccaTGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGATCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGc                                                                                      <  3:928516/140‑1 (MQ=255)
                                                    ccaTGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGc                                                                                      <  2:555847/138‑1 (MQ=255)
                                                    ccaTGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGAGC‑GCCGTTACCAGCGCCAGACACTCGCCCTTCACCCCC‑CCCACCCCCCGAACGCGAAAGCCa                                                                                    >  6:651765/1‑140 (MQ=255)
                                                     caTGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGGTTTTGAGC‑CCCCGTTCCACCGCCCACCCCTGGCCGGTAACCACC‑CCCACCCCCGGACCGCGAAAGcccc                                                                                   >  5:192790/1‑138 (MQ=255)
                                                      aTGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCGCGTTCGAGCTGGAGTCGTGTTTTACC‑GCCTGTTCCCGCGCCCTCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCGTAAGCCa                                                                                    >  2:19867/1‑138 (MQ=255)
                                                      aTGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTCCC‑GCCTGTACCAGCGCCCTCCAGTCGCCTTTCACCACC‑CCCACCCCCGTACCGCTAAAGccc                                                                                    >  2:665514/1‑137 (MQ=255)
                                                               gCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTAAC‑CCCTGTACCACCGCCCAACACTCGCCATTAACCCCC‑CCCACCCCCCGACCCCGAAAGCCACAAAGAAGCTg                                                                         >  3:127838/1‑140 (MQ=255)
                                                               gCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTTGCC‑GCCGGGAACAGCGGCCTCCCGTCGGCATTCACCCCGCCCAACCCCCGT‑CCCCGAAATCCAGAAATAATCTg                                                                         >  3:119407/1‑140 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTgag                                                                       <  3:811038/140‑1 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTg                                                                         <  2:85712/138‑1 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTg                                                                         <  1:1086982/138‑1 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTTTCC‑GCCGGTACCCGCGCCATCCAGTCGCCGTTCACCACG‑CCCACCGCCGTACCCCTAAAGCCAGAAAGAAGCTg                                                                         >  2:570286/1‑138 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGCCC‑CCCTGTACCCGCGCCAGCCCGTCGCCCTTCACCCCC‑CCCACCCCCGGACCGCTAAACCAAGAAAGCAGCTg                                                                         >  1:783630/1‑138 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCAGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTgag                                                                       <  7:931152/140‑1 (MQ=255)
                                                                 ttCCTGGTTGTCCAGCGGGGGGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTgag                                                                       <  8:709848/140‑1 (MQ=255)
                                                                 ttCCTGGTTGGCCAGCGTGGTGATACTCGGCGGGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTg                                                                         <  1:416393/138‑1 (MQ=255)
                                                                 ttCCGGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGGCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTg                                                                         <  2:994437/138‑1 (MQ=255)
                                                                   ccTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTgagcg                                                                     <  5:43438/140‑1 (MQ=255)
                                                                   ccTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGACC‑CCCCGTACCCGCGCCCTCCCGTCGCCGTTCACCCCC‑CCCACCGCCGTACCGCTAAAGCCAGAAAAAAGCAgagcg                                                                     >  3:743212/1‑140 (MQ=255)
                                                                     gggTTGTCCAGCGTGGGGATACTCGGCGTGGAGAGCCCGTGCTAGCTGGAGGCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTgagcgag                                                                   <  6:1036935/139‑1 (MQ=255)
                                                                      ggTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTgagcga                                                                    <  1:985335/138‑1 (MQ=255)
                                                                         tgaccAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTTACC‑GCCTGTACCAGCGCCATACAGTCGCCTTTAACCACG‑CCAACCCCCGTACCGCAAAAGCCAGAAAGAACCTGAGCGAGCGTg                                                               >  4:448120/4‑140 (MQ=255)
                                                                          gTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACA‑CCCGGTACCAGCGCCAACCAGTCGCCATTCACCACG‑CCCACCCCCGTGCCCCAAAACCCAGAAAGACGCGGAGCCAGCGTgg                                                              >  4:966734/1‑140 (MQ=255)
                                                                          gTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTGTCC‑GCCGGTTCCCCCGCCCACCAGGCGCCGTTCACCCCG‑CCCCCCCCCGGGCCCCGAAAGCCAGCAAGCCGCTGAGCGCGCgggg                                                              >  5:894402/1‑140 (MQ=255)
                                                                           tCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTGTTTTGCC‑GCCGGTACCACCGCCCTCCCGTCGCCGTTGCCCCCG‑CCCACCCCCGGTCCGCGAAAGCCAGAAAGAAGGGGAGCGCGCggggg                                                             >  8:193592/1‑139 (MQ=255)
                                                                           tCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGATGGAGTCGTTTTTTACC‑GCCAGGACCAGCGCCATCCACTCGCCTTTCACCACC‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAACCGGCGCGAGCGttgt                                                             >  6:963862/1‑137 (MQ=255)
                                                                              cgCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGgagct                                                          <  3:835570/139‑3 (MQ=255)
                                                                              aGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGgagag                                                          <  6:250164/140‑1 (MQ=255)
                                                                              aGCGGGGTGATCCTCGGCGGGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCATAAAGAAGCTGAGCGAGCGTGgagag                                                          <  8:612592/140‑1 (MQ=255)
                                                                                cGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGGTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGgagag                                                          <  2:277396/138‑1 (MQ=255)
                                                                                  tggtgATACTCGGCGTGGATAGCACGTTCGAGCTGGAGTCGTGTTTTGCC‑GCCTGGACCAGCGGCCACCACTCGGCGTTTAACCCG‑CCCACCGGCGGAACGCGAAAGCCAGAAAGAAGCTGAGCGGGGGGGGAGAGAt                                                        >  2:426587/1‑138 (MQ=255)
                                                                                  aggtgATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCAGTACCACCCCCCAACACTAGCCATTAACCACC‑CCAACCGCCGTACCGCAAAAACCAGAAATAAGATGAGCAAACGGGGAGCGAt                                                        >  2:784821/2‑138 (MQ=255)
                                                                                   ggtgATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGc                                                     <  3:594066/140‑1 (MQ=255)
                                                                                   ggtgATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGc                                                     <  7:773797/140‑1 (MQ=255)
                                                                                   ggtgATACTCGGCGTGGAGAGCACGTGCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGc                                                     <  3:993924/140‑1 (MQ=255)
                                                                                             ggCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATc                                           <  3:834275/140‑1 (MQ=255)
                                                                                             ggCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATc                                           <  5:651765/140‑1 (MQ=255)
                                                                                             ggCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATc                                           <  4:146400/140‑1 (MQ=255)
                                                                                             ggCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCATTTGACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTa                                             <  1:1065594/138‑1 (MQ=255)
                                                                                             ggCGGGGAGTGCACGTTCGTGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CAAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATc                                           <  4:743212/140‑1 (MQ=255)
                                                                                                gTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGGCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCgg                                        <  5:64872/140‑1 (MQ=255)
                                                                                                 gggAGAGCACGATCGAGATGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCggg                                       <  3:874204/139‑1 (MQ=255)
                                                                                                   gagaGCACGTTTTAGCTGGAGTCGTTTTTGACC‑GCTTGTACCTGCGCAATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGTAGCTGAGCGTGCGTGGAGAGATCGCAGTTAGTATCCGGGtt                                     <  8:340755/140‑1 (MQ=255)
                                                                                                      agCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCTCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAAt                                  <  6:222619/140‑1 (MQ=255)
                                                                                                       gCACGTTCGAGCTGGAGTCGTGTTTTACC‑GCCTGTACCACCGCCATCCACGCGCCGTTCACCCCG‑CCAAAAGCCCTACCCCAAAATCCAGCAAGAATCGTAGCGAGCGTGGCGAGCTCGCCCTTATTATACGGGTTAAtg                                 >  4:1045176/1‑140 (MQ=255)
                                                                                                        cACGTTCGAGCTGGAGTCGTTTTTTACC‑GCCTGTACCAGCGCCATCCAGTCGCCGTTCAACACG‑CCAACCCCCGGACCGCGAAAGCCAGAAATAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATACGGGTTTAttg                                >  6:446705/1‑138 (MQ=255)
                                                                                                               gAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATGCTGTCGCCTTTAACGACG‑GCATCCGCCGTACCGGTAAAGCCAGTATGAAGCTGAGCGAGCGTGGAGAGATCGCAGGTAGTATCCGGGTTAATGGTGGTAga                         <  6:443407/140‑2 (MQ=255)
                                                                                                               gAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAgc                         <  7:55349/140‑1 (MQ=255)
                                                                                                                  cTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTTATTGTGGTAgcg                        >  2:587794/1‑138 (MQ=255)
                                                                                                                  cTGGAGTCGTTTTTGACC‑GCCGGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGcc                      <  7:147593/140‑1 (MQ=255)
                                                                                                                  cTGGAGTCGTTTTTGACC‑GCCGGTACCAGCGCCATCCAGTCGCCGTTCACCACG‑CAAACCGCCGTACCGCTAAAGCCAGAAAGAAGATGAGCGAGCGTGGAGAGCTCGCCGTTAGTATCCTGGTTAATGGTGGTAGCGcc                      <  8:459218/140‑1 (MQ=255)
                                                                                                                    ggAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGt                    <  4:185933/140‑1 (MQ=255)
                                                                                                                      aGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGttt                  <  3:1023715/140‑1 (MQ=255)
                                                                                                                         cGTATTGGACC‑GCCTGTACCTGCGCCATCCAGTCGCCTTTCACCACT‑CCAACCGCCGTACCGCTAAAGCCAGTAAGAAGCTGAGCGAGCGTGGAGAGAGCGCAGTTAGTATCCGGGGTAATGGTGGTAGCGCCGTTTTCa               <  7:829457/140‑1 (MQ=255)
                                                                                                                              ttGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGAGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGAt          <  8:526132/140‑1 (MQ=255)
                                                                                                                              ttGACA‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAATGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGAt          <  6:555963/140‑1 (MQ=255)
                                                                                                                              tggACC‑GCCTGAACCAGCGCCATCCAGTCGCCTTTCAACACG‑CCAACCGCCGTACCGCTAAAGCAAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGAt          <  6:622916/138‑1 (MQ=255)
                                                                                                                                 aCC‑GCCTGTACCAACCCCAAGCAATCGCATTTCACCACG‑CCAAACCCCGTAACCCTAAAGACACTAAGAATCAGAGCGCGCGTGGAGAAATAACCGGTACTAACCGGGTATATGGTGGTAGCGCCGTTTTCAcggcag         >  2:885685/1‑133 (MQ=255)
                                                                                                                                     gCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGCTCACCGt    >  7:297191/1‑140 (MQ=255)
                                                                                                                                       cTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGATCACCGTgg  <  3:971706/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
CTGTTATTAGAGCCAACAGTAGAGCCGGTTAATACCGGAACGTCCTGGCCCACCATGAAGAAGGCTTCCTGGTTGTCCAGCGTGGTGATACTCGGCGTGGAGAGCACGTTCGAGCTGGAGTCGTTTTTGACC‑GCCTGTACCAGCGCCATCCAGTCGCCTTTCACCACG‑CCAACCGCCGTACCGCTAAAGCCAGAAAGAAGCTGAGCGAGCGTGGAGAGATCGCCGTTAGTATCCGGGTTAATGGTGGTAGCGCCGTTTTCACTGATCACCGTGG  >  NZ_CP022686/1833621‑1833894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A