Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I229 R1
|
214 |
17.4 |
943020 |
97.5% |
919444 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,131,004 |
A→G |
intergenic (‑280/‑379) |
wza ← / → yegH |
polysaccharide export protein Wza/TerC family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,131,004 | 0 | A | G | 100.0%
| 43.3
/ NA
| 15 | intergenic (‑280/‑379) | wza/yegH | polysaccharide export protein Wza/TerC family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (4/11); total (4/11) |
TTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTA > NZ_CP009273/2130922‑2131078
|
ttttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAAc < 1:146940/90‑1 (MQ=255)
aTACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGTATATaa < 2:171176/90‑1 (MQ=255)
cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt < 1:320188/90‑1 (MQ=255)
cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt < 2:20374/90‑1 (MQ=255)
cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt < 2:461717/90‑1 (MQ=255)
gTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATgg > 2:287826/1‑90 (MQ=255)
ttAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTa > 2:245215/1‑90 (MQ=255)
tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCttt < 2:231680/90‑1 (MQ=255)
gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg > 2:441657/1‑90 (MQ=255)
cAGTCGTTGATGAGAAAGGGTTATTACGGGAATTa < 1:51164/35‑1 (MQ=39)
cAGTCGTTGATGAGAAAGGGTTATTACGGGAATTa > 2:51164/1‑35 (MQ=39)
aGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCa < 2:100135/90‑1 (MQ=255)
gTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCaggag < 1:277495/90‑1 (MQ=255)
atgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAg < 1:170524/90‑1 (MQ=255)
aaaGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTa < 1:181622/90‑1 (MQ=255)
|
TTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTA > NZ_CP009273/2130922‑2131078
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGA > NZ_CP009273/2130920‑2131089
|
gattcgccttagtctcgtgggctcggagatgtgtataagagacagTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAAT < SRR3722113.51590/55‑1 (MQ=60)
TTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATAT < SRR3722113.148151/100‑1 (MQ=60)
CACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGG < SRR3722113.323347/100‑1 (MQ=60)
GTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTAC < SRR3722113.279951/100‑1 (MQ=60)
ATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCT < SRR3722113.171899/100‑1 (MQ=60)
AAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGG < SRR3722113.183055/100‑1 (MQ=60)
AGGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGA < SRR3722113.314280/100‑1 (MQ=60)
|
CTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGA > NZ_CP009273/2130920‑2131089
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
