Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I97 R1
|
275 |
44.0 |
1733530 |
96.5% |
1672856 |
122.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
974,173 |
T→C |
S798P (TCC→CCC) |
mukB → |
chromosome partition protein MukB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 974,173 | 0 | T | C | 100.0%
| 78.4
/ NA
| 23 | S798P (TCC→CCC) | mukB | chromosome partition protein MukB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/11); total (12/11) |
GAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACTGAA > NZ_CP009273/974044‑974306
|
gAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt < 2:109096/139‑1 (MQ=255)
ggTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg < 2:454820/139‑1 (MQ=255)
ggTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg < 1:813801/139‑1 (MQ=255)
tAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg > 2:100693/1‑137 (MQ=255)
tAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg < 1:100693/137‑1 (MQ=255)
cGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCtctc < 2:698240/113‑1 (MQ=255)
cGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCtctc > 1:698240/1‑113 (MQ=255)
cGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTc > 2:856957/1‑139 (MQ=255)
tCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGtt > 1:858859/1‑139 (MQ=255)
ccGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTa > 1:628254/1‑139 (MQ=255)
ctgcGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGc > 1:465368/1‑139 (MQ=255)
cgcgTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCgg < 2:372083/138‑1 (MQ=255)
tATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct < 2:168875/54‑1 (MQ=255)
tATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct > 1:168875/1‑54 (MQ=255)
tATTGAAAGCCTCCATGCCGAGCGCGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCt > 1:613334/1‑139 (MQ=255)
aaaGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGAccc < 2:410317/139‑1 (MQ=255)
aGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGt > 2:139543/1‑62 (MQ=255)
aGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGt < 1:139543/62‑1 (MQ=255)
cGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAg > 1:828506/1‑69 (MQ=255)
cGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAg < 2:828506/69‑1 (MQ=255)
cGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGt > 1:832101/1‑138 (MQ=255)
cGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGt > 2:528466/1‑138 (MQ=255)
tGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACTGaa < 1:803934/139‑1 (MQ=255)
|
GAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACTGAA > NZ_CP009273/974044‑974306
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACTGAACAGCCGTCGCG > NZ_CP009273/974025‑974317
|
GTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCC > SRR3722120.377584/1‑151 (MQ=60)
cgctgatatgttgtgctctccatcggtttttttcaagcagaagacggcatacgatattcgccttagtctcgtgggctctgagatgtgtataagagacAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTT < SRR3722120.418740/53‑1 (MQ=60)
CGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGA < SRR3722120.102039/151‑1 (MQ=60)
CGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGA < SRR3722120.824761/151‑1 (MQ=60)
ATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACctgtctcttatacac > SRR3722120.707771/1‑135 (MQ=60)
caagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGC < SRR3722120.141430/86‑1 (MQ=60)
TTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTC > SRR3722120.870347/1‑151 (MQ=60)
TCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTAT > SRR3722120.636958/1‑151 (MQ=60)
GTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCG > SRR3722120.472120/1‑151 (MQ=60)
CGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcttctgcttgaaaaaaaa > SRR3722120.171183/1‑76 (MQ=60)
CGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGCGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTG > SRR3722120.621874/1‑151 (MQ=60)
TCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTgtctcttatacacatctccgagcccacgagactaaggcgaatctagtatgccgtcttc > SRR3722120.839645/1‑93 (MQ=60)
TCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTC > SRR3722120.843276/1‑150 (MQ=60)
GTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACTGAACAGCCGTCGCG < SRR3722120.814784/151‑1 (MQ=60)
|
GTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACTGAACAGCCGTCGCG > NZ_CP009273/974025‑974317
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
