Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I88 R1
|
217 |
30.5 |
1742422 |
95.9% |
1670982 |
87.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
974,173 |
T→C |
S798P (TCC→CCC) |
mukB → |
chromosome partition protein MukB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 974,173 | 0 | T | C | 100.0%
| 125.5
/ NA
| 33 | S798P (TCC→CCC) | mukB | chromosome partition protein MukB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/20); total (13/20) |
TATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGC > NZ_CP009273/974086‑974250
|
tATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCg > 1:435688/1‑91 (MQ=255)
tCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAAc > 2:747051/1‑91 (MQ=255)
aCGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGc < 2:455684/91‑1 (MQ=255)
cGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt > 2:455858/1‑91 (MQ=255)
cGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt > 2:259248/1‑91 (MQ=255)
cGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt > 1:684221/1‑91 (MQ=255)
tCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGc < 2:711350/91‑1 (MQ=255)
cGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCAc < 2:85985/91‑1 (MQ=255)
cGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCAc < 1:459667/91‑1 (MQ=255)
gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc < 2:703489/91‑1 (MQ=255)
gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc < 1:761750/91‑1 (MQ=255)
gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc < 2:296914/91‑1 (MQ=255)
gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc < 2:684221/91‑1 (MQ=255)
aGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc < 2:243490/91‑1 (MQ=255)
aGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc < 1:35702/91‑1 (MQ=255)
gCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTcc < 2:687663/91‑1 (MQ=255)
gCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTcc < 1:377735/91‑1 (MQ=255)
cGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCtt < 1:118329/91‑1 (MQ=255)
tGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTa < 1:424222/91‑1 (MQ=255)
gTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACa > 1:571635/1‑91 (MQ=255)
gTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc < 2:273842/74‑1 (MQ=255)
gTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc > 1:273842/1‑74 (MQ=255)
gctgcGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGaaaa > 2:350209/1‑91 (MQ=255)
cgcgTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCa < 2:343257/91‑1 (MQ=255)
aTTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAgg < 2:571635/91‑1 (MQ=255)
ccTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGc > 2:459408/1‑91 (MQ=255)
cTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGcc < 2:601524/91‑1 (MQ=255)
tCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCg < 2:535467/91‑1 (MQ=255)
tCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCg > 1:639013/1‑91 (MQ=255)
cGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCTGGCGTTCAGCCGCTTTATc > 1:297060/1‑91 (MQ=255)
cGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAAAGTCTGCATCAGGCGTTCAGCCGCTTTATc > 2:783573/1‑91 (MQ=255)
gCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGc < 1:310170/91‑1 (MQ=255)
gCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCGGCATCACGAGTTCAGCCGCTTTAtgtgc > 2:201905/1‑87 (MQ=255)
|
TATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGC > NZ_CP009273/974086‑974250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTT > NZ_CP009273/974076‑974271
|
TCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCG > SRR3722118.444882/1‑101 (MQ=60)
GCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCT > SRR3722118.699093/1‑101 (MQ=60)
CGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTT < SRR3722118.469369/101‑1 (MQ=60)
GAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGA < SRR3722118.778483/101‑1 (MQ=60)
AGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATG < SRR3722118.36350/101‑1 (MQ=60)
GCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTAC < SRR3722118.385629/101‑1 (MQ=60)
CCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACA > SRR3722118.583757/1‑101 (MQ=60)
CGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAG < SRR3722118.120549/101‑1 (MQ=60)
CTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGctgtctc > SRR3722118.279451/1‑94 (MQ=60)
TGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTC < SRR3722118.433188/101‑1 (MQ=60)
ATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCG > SRR3722118.652806/1‑101 (MQ=60)
GCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCTGGCGTTCAGCCGCTTTATC > SRR3722118.303149/1‑101 (MQ=60)
GCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGG < SRR3722118.316544/101‑1 (MQ=60)
CTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTT > SRR3722118.279840/1‑101 (MQ=60)
TTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTT > SRR3722118.443744/1‑101 (MQ=60)
|
TCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTT > NZ_CP009273/974076‑974271
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
