Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I179 R1
|
182 |
40.3 |
1547118 |
96.8% |
1497610 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
634,370 |
A→C |
intergenic (‑341/‑31) |
dsbG ← / → ahpC |
thiol:disulfide interchange protein DsbG/alkyl hydroperoxide reductase subunit C |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 634,370 | 0 | A | C | 100.0%
| 104.6
/ NA
| 27 | intergenic (‑341/‑31) | dsbG/ahpC | thiol:disulfide interchange protein DsbG/alkyl hydroperoxide reductase subunit C |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (12/15); total (12/15) |
AGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCG > NZ_CP009273/634237‑634503
|
aGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCAtct > 2:237654/1‑139 (MQ=255)
ggggCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCa > 2:572228/1‑139 (MQ=255)
ggCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATc > 2:478413/1‑139 (MQ=255)
cTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACgg < 2:704609/139‑1 (MQ=255)
tttATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACgg < 2:468323/136‑1 (MQ=255)
ttATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACggagg < 1:433143/138‑1 (MQ=255)
ttATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACggagg < 1:692991/138‑1 (MQ=255)
ccgcTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGta > 1:369106/1‑137 (MQ=255)
gcTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGa < 2:769554/139‑1 (MQ=255)
cacaAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAacac > 2:744010/1‑138 (MQ=255)
gTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGaaaa < 1:123466/85‑1 (MQ=255)
gTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGaaaa > 2:123466/1‑85 (MQ=255)
gTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAAcc < 2:92969/138‑1 (MQ=255)
tCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTa < 1:742345/108‑1 (MQ=255)
tCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTa > 2:742345/1‑108 (MQ=255)
ttGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACggagg > 1:648635/1‑70 (MQ=255)
ttGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACggagg < 2:648635/70‑1 (MQ=255)
gATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTTCCTTCCATCTCATCGAAAACACGGAGGaa < 2:438223/70‑1 (MQ=255)
gATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGaa > 1:438223/1‑70 (MQ=255)
tGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCg > 1:560672/1‑138 (MQ=255)
aaaCGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAAt < 2:560672/139‑1 (MQ=255)
ttAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGaa < 2:224385/139‑1 (MQ=255)
tAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGaa > 1:312777/1‑138 (MQ=255)
gCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGaa < 2:6070/139‑1 (MQ=255)
cAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAg > 2:410005/1‑139 (MQ=255)
gTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGa < 1:572228/139‑1 (MQ=255)
ccTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCg < 1:478413/138‑1 (MQ=255)
|
AGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCG > NZ_CP009273/634237‑634503
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGGTCACCTGGAAAGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTC > NZ_CP009273/634224‑634514
|
agacagCCTGGAAAGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATC < SRR3722133.223367/145‑1 (MQ=60)
agtacggtctcgtgggctcggagatgtgtataagagacagGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAG < SRR3722133.125096/107‑1 (MQ=60)
ACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTAT > SRR3722133.374277/1‑149 (MQ=60)
TTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATG < SRR3722133.439065/150‑1 (MQ=60)
TTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATG < SRR3722133.701985/150‑1 (MQ=60)
ggagatgtgtataagagacagGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAA < SRR3722133.751941/130‑1 (MQ=60)
AACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtctt > SRR3722133.657101/1‑92 (MQ=60)
CTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTgtctcttatacacatctccgagcccacgagaccgtactagatatcgtatgccgaatt > SRR3722133.444196/1‑94 (MQ=60)
CGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGA > SRR3722133.568128/1‑150 (MQ=60)
TGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAA > SRR3722133.317076/1‑150 (MQ=60)
GGTTACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTC < SRR3722133.579803/151‑1 (MQ=60)
ACCTTCCATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTC < SRR3722133.484890/150‑1 (MQ=60)
|
CGGTCACCTGGAAAGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTC > NZ_CP009273/634224‑634514
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
