Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I176 R1	180	42.9	1710034	96.5%	1650182	119.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	547,771	T→C	T94A (ACT→GCT)	purK ←	5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	547,771	0	T	C	100.0%	175.6 / NA	47	T94A (ACT→GCT)	purK	5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/22); total (25/22)

TTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATC  >  NZ_CP009273/547636‑547904

tttCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCgg                                                                                                                                    <  1:105681/139‑1 (MQ=255)

tttCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCgg                                                                                                                                    <  1:669491/139‑1 (MQ=255)

  tCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGc                                                                                                                                   <  1:480826/138‑1 (MQ=255)

       tGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTg                                                                                                                              <  2:126370/138‑1 (MQ=255)

                      ttGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTg                                                                                                                              <  2:786379/123‑1 (MQ=255)

                      ttGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTg                                                                                                                              >  1:786379/1‑123 (MQ=255)

                      ttGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgc                                                                                                              >  1:692224/1‑139 (MQ=255)

                        gACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgct                                                                                                             <  2:482141/138‑1 (MQ=255)

                              gACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCt                                                                                                       >  2:649177/1‑138 (MQ=255)

                                            ccaGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                        >  2:283926/1‑139 (MQ=255)

                                             caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                        >  2:854408/1‑138 (MQ=255)

                                             caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                        >  1:762780/1‑138 (MQ=255)

                                              aGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCa                                                                                      <  1:649177/139‑1 (MQ=255)

                                               gTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaa                                                                                     <  2:692224/139‑1 (MQ=255)

                                                  cGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaataa                                                                                  >  2:659859/1‑139 (MQ=255)

                                                                 gCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcgc                                                                   >  1:548361/1‑139 (MQ=255)

                                                                 gCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcgc                                                                   >  1:514587/1‑139 (MQ=255)

                                                                 gCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcg                                                                    <  2:686038/138‑1 (MQ=255)

                                                                  ccAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcgcg                                                                  >  1:695079/1‑139 (MQ=255)

                                                                      cTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGtt                                                               >  1:203813/1‑138 (MQ=255)

                                                                                cGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaataat                                                                                 >  2:356037/1‑110 (MQ=255)

                                                                                cGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaataat                                                                                 <  1:356037/110‑1 (MQ=255)

                                                                                   tCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGAt                                                 >  2:55622/1‑139 (MQ=255)

                                                                                   tCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGAt                                                 >  1:115910/1‑139 (MQ=255)

                                                                                     aaaCACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcac                                                            <  2:334249/126‑1 (MQ=255)

                                                                                     aaaCACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcac                                                            >  1:334249/1‑126 (MQ=255)

                                                                                            gCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcg                                                                    >  2:461005/1‑111 (MQ=255)

                                                                                            gCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcg                                                                    <  1:461005/111‑1 (MQ=255)

                                                                                             cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                       >  1:836797/1‑139 (MQ=255)

                                                                                             cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                       >  2:453757/1‑139 (MQ=255)

                                                                                             cAGGCCACTCGCTGCGATCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                       >  1:822109/1‑139 (MQ=255)

                                                                                                gCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTc                                    <  1:659859/139‑1 (MQ=255)

                                                                                                 ccACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTcg                                   <  1:283926/139‑1 (MQ=255)

                                                                                                     tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                               >  1:589947/1‑139 (MQ=255)

                                                                                                     tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                               >  2:680282/1‑139 (MQ=255)

                                                                                                            gTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGc                        <  2:548361/139‑1 (MQ=255)

                                                                                                             ttCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCg                       >  2:831112/1‑139 (MQ=255)

                                                                                                                  cAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgc                                                                                                              >  1:804453/1‑47 (MQ=255)

                                                                                                                  cAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgc                                                                                                              <  2:804453/47‑1 (MQ=255)

                                                                                                                      tAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc              <  2:822109/139‑1 (MQ=255)

                                                                                                                      tAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc              <  2:848523/139‑1 (MQ=255)

                                                                                                                        aCTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGa                                                                                               <  1:2979/56‑1 (MQ=255)

                                                                                                                        aCTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGa                                                                                               >  2:2979/1‑56 (MQ=255)

                                                                                                                        aCTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGcc             <  2:695079/138‑1 (MQ=255)

                                                                                                                         cTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAg           <  1:55622/139‑1 (MQ=255)

                                                                                                                              aCGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTc      <  2:589947/139‑1 (MQ=255)

                                                                                                                                  tGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAtc  >  1:623764/1‑139 (MQ=255)

TTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATC  >  NZ_CP009273/547636‑547904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCA  >  NZ_CP009273/547635‑547917

GTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCT                                                                                                                                      <  SRR3722130.107172/151‑1 (MQ=60)

GTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCT                                                                                                                                      <  SRR3722130.679446/151‑1 (MQ=60)

  TTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTT                                                                                                                                     <  SRR3722130.488271/150‑1 (MQ=60)

            CGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGActgtct                                                                                                                          >  SRR3722130.797850/1‑145 (MQ=60)

            CGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCT                                                                                                                          >  SRR3722130.702479/1‑151 (MQ=60)

                                   GTCACAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGC                                                                                                    >  SRR3722130.773918/1‑150 (MQ=60)

                                      ttttttcaagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAAT                                                                                                 <  SRR3722130.3024/79‑1 (MQ=60)

                                              CAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAA                                                                                        <  SRR3722130.658899/151‑1 (MQ=60)

                                                   agatgtgtataagagacagGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACAT                                                                                   <  SRR3722130.361573/132‑1 (MQ=60)

                                                       GCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCG                                                                               >  SRR3722130.522487/1‑151 (MQ=60)

                                                       GCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCG                                                                               >  SRR3722130.556700/1‑151 (MQ=60)

                                                        CTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGG                                                                              >  SRR3722130.705370/1‑151 (MQ=60)

                                                            ACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTC                                                                           >  SRR3722130.206913/1‑150 (MQ=60)

                                                                gatgtgtataagagacagGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAA                                                                      <  SRR3722130.468196/133‑1 (MQ=60)

                                                                         CACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATG                                                             >  SRR3722130.117545/1‑151 (MQ=60)

                                                                           CCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATctg                                                           >  SRR3722130.339417/1‑148 (MQ=60)

                                                                                   ATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGC                                                   >  SRR3722130.848930/1‑151 (MQ=60)

                                                                                   ATCAAACACCGCAGGCCACTCGCTGCGATCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGGCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGC                                                   >  SRR3722130.834060/1‑151 (MQ=60)

                                                                                           CCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGT                                           >  SRR3722130.598835/1‑151 (MQ=60)

                                                                                                GGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATG                                      <  SRR3722130.669713/151‑1 (MQ=60)

                                                                                                 GCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGC                                     <  SRR3722130.288345/151‑1 (MQ=60)

                                                                                                        GCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCtgtctcttatacacatctccgagcccacgagactaaggcgaatatcgtatgccgtcttctgcttgaaaaaaacaaaag                                 >  SRR3722130.816201/1‑70 (MQ=60)

                                                                                                                        AACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCT              >  SRR3722130.633120/1‑151 (MQ=60)

                                                                                                                         ACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTC             <  SRR3722130.56385/151‑1 (MQ=60)

                                                                                                                                  GTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAAT    >  SRR3722130.217987/1‑151 (MQ=60)

                                                                                                                                    GCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCA  <  SRR3722130.532154/151‑1 (MQ=60)

GTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCA  >  NZ_CP009273/547635‑547917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`