Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I176 R1
|
180 |
42.9 |
1710034 |
96.5% |
1650182 |
119.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
753,637 |
A→G |
N165D (AAT→GAT) |
sdhB → |
succinate dehydrogenase iron‑sulfur subunit SdhB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 753,637 | 0 | A | G | 97.7%
| 149.6
/ ‑6.4
| 44 | N165D (AAT→GAT) | sdhB | succinate dehydrogenase iron‑sulfur subunit SdhB |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (25/18); total (25/19) |
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
TGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACA > NZ_CP009273/753509‑753766
|
tGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 1:690730/1‑137 (MQ=255)
gAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAg > 1:695234/1‑139 (MQ=255)
aaaaTCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGccc > 2:621341/1‑139 (MQ=255)
tttACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat < 2:839079/139‑1 (MQ=255)
gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 2:404966/1‑94 (MQ=255)
gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt < 1:404966/94‑1 (MQ=255)
gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATTTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat < 1:644065/139‑1 (MQ=255)
gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat < 1:281006/139‑1 (MQ=255)
gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat < 2:796527/139‑1 (MQ=255)
agcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 2:103191/139‑1 (MQ=255)
cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTTCTGATTGATAGcc > 1:680512/1‑139 (MQ=255)
cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCGTGATTGATTGcc > 1:662775/1‑139 (MQ=255)
cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTTATAGcc > 1:328484/1‑139 (MQ=255)
cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:229423/1‑139 (MQ=255)
cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:116559/1‑139 (MQ=255)
cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 2:662002/1‑139 (MQ=255)
tATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAgc < 1:662002/138‑1 (MQ=255)
aTGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTc < 2:229423/139‑1 (MQ=255)
tGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCg < 2:116559/139‑1 (MQ=255)
tATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc > 1:256888/1‑139 (MQ=255)
ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGGTTGATAg > 2:671905/1‑106 (MQ=255)
ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 1:671905/106‑1 (MQ=255)
ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACa < 1:122775/126‑1 (MQ=255)
ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACa > 2:122775/1‑126 (MQ=255)
ctctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGTTTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGt < 1:191986/138‑1 (MQ=255)
ctctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGtt > 2:126046/1‑139 (MQ=255)
ctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGGTTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTg > 1:219755/1‑139 (MQ=255)
ctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGGGATACCGAGACTGACAGCCGCCTCGACGGTTTg > 2:613832/1‑139 (MQ=255)
tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt < 1:76931/48‑1 (MQ=255)
tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 2:76931/1‑48 (MQ=255)
tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTATCGGCATATCGTTTTCTGATTTATAGCCGTGATTCCGAGTATGACAGCCGCCTCGACGGgtt > 2:474489/1‑137 (MQ=255)
gcgcATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCCGGCTTGTTAGCGGCATATCGTTTCCTTATTTATAGCCGTGATACCGAGACTTACAGCCGCCTCGACGGTTTGAg > 2:86924/1‑139 (MQ=255)
cccATGTTGTTCAACCTCTTGTCCATCTTTCTGGTGGAATCCCGATAAGTTTAGCGGCCCGGCAGGCTTGTTAGCCGCATGTCGTTTCCGGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGa < 1:193477/135‑1 (MQ=255)
ccTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGGGACTGACAGCCGCCCCGACGGGTTGAGTGATGCATTCAGCg > 2:437953/1‑138 (MQ=255)
ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 1:790508/82‑1 (MQ=255)
ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg > 2:790508/1‑82 (MQ=255)
ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTAtt > 1:138634/1‑139 (MQ=255)
cGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTg > 1:736058/1‑139 (MQ=255)
tCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc > 2:70225/1‑105 (MQ=255)
tCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc < 1:70225/105‑1 (MQ=255)
cTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACGGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTca < 2:476637/139‑1 (MQ=255)
ttCTGGTGGGATCCCGATAAGTTTATCGGCCCGGGAGGCCTGTTTGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTTAGCGGATTTCGCTGTca > 2:8402/1‑137 (MQ=255)
tCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcaca < 2:256888/138‑1 (MQ=255)
tCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcaca < 1:616701/138‑1 (MQ=255)
|
TGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACA > NZ_CP009273/753509‑753766
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTC > NZ_CP009273/753498‑753783
|
GCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATA > SRR3722130.700962/1‑149 (MQ=60)
CCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGT > SRR3722130.705527/1‑151 (MQ=60)
agtctcgtgggctcggagatgtgtataagagacAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGG < SRR3722130.411398/118‑1 (MQ=60)
ttttttttttttttcaagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGG < SRR3722130.78028/71‑1 (MQ=60)
CATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGTTTTTTAACGGCATATCGTTTCCCG > SRR3722130.801936/1‑150 (MQ=60)
TGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTTCTGATTGATAGCCG > SRR3722130.690620/1‑151 (MQ=60)
TGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCGTGATTGATTGCCG > SRR3722130.672660/1‑151 (MQ=60)
TGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTTATAGCCG > SRR3722130.333558/1‑151 (MQ=60)
TGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCG > SRR3722130.118208/1‑151 (MQ=60)
TGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCG > SRR3722130.232946/1‑151 (MQ=60)
AGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATTTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATAC < SRR3722130.653714/151‑1 (MQ=60)
AGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATAC < SRR3722130.285343/151‑1 (MQ=60)
gagattcgccttagtctcgtgggctcggagatgtgtataagagacaGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCG < SRR3722130.802035/105‑1 (MQ=60)
cggagatgtgtataagagacagGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCG < SRR3722130.681890/128‑1 (MQ=60)
cagGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAC < SRR3722130.124529/148‑1 (MQ=60)
CTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGGGATACCGAGACTGACAGCCGCCTCGACG > SRR3722130.260904/1‑151 (MQ=60)
gctcggagatgtgtataagagacaGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGG < SRR3722130.57762/127‑1 (MQ=60)
GTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGG < SRR3722130.671882/150‑1 (MQ=60)
GAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGGTTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGG > SRR3722130.223065/1‑151 (MQ=60)
cggagatgtgtataagagacagCCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGA < SRR3722130.71219/127‑1 (MQ=60)
TCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGTTTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGC < SRR3722130.194882/150‑1 (MQ=60)
GCCCATGTTGTTCAACCTCTTGTCCATCTTTCTGGTGGAATCCCGATAAGTTTAGCGGCCCGGCAGGCTTGTTAGCCGCATGTCGTTTCCGGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTC < SRR3722130.196423/149‑1 (MQ=60)
TTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTC > SRR3722130.140694/1‑151 (MQ=60)
AACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGGGTGGTTCATTCAGCGTATTCCGCTGG > SRR3722130.746862/1‑151 (MQ=60)
CTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTTTTAGCGGCATATCGTTTCCTGCTTGTTAACCGTGATACCGCGACTGACCGCCGCCTCGACGGTTTTAGTGATGCATTCAGCGTATTCCGCTGTCAC > SRR3722130.123969/1‑151 (MQ=60)
TTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGGTTATTAACGGCCTATCGATTTCTGATTAATAGGCGTGATAACGAGACTGACAGCCGGCTCGGCGGGTTGGGTGATGGATTAAGGGTATTCCGATGTCACAGgctcattaa > SRR3722130.343122/1‑141 (MQ=41)
TTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAAC > SRR3722130.27776/1‑151 (MQ=60)
TTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAAC < SRR3722130.625968/150‑1 (MQ=60)
GTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTC < SRR3722130.176218/151‑1 (MQ=60)
|
GCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTC > NZ_CP009273/753498‑753783
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
