Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I102 R1
|
278 |
39.1 |
2251254 |
95.8% |
2156701 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
716,126 |
A→G |
intergenic (‑210/+48) |
speF ← / ← BW25113_RS26090 |
ornithine decarboxylase SpeF/leader peptide SpeFL |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 716,126 | 0 | A | G | 100.0%
| 128.3
/ NA
| 34 | intergenic (‑210/+48) | speF/BW25113_RS26090 | ornithine decarboxylase SpeF/leader peptide SpeFL |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/13); total (21/13) |
ATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATACAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGC > NZ_CP009273/716039‑716211
|
aTGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCag < 2:208467/91‑1 (MQ=255)
ttACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAg > 2:621358/1‑91 (MQ=255)
ccAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGAcc < 1:558913/91‑1 (MQ=255)
tCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTa > 2:83554/1‑91 (MQ=255)
ggCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGa > 2:232442/1‑91 (MQ=255)
cTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAg < 2:896666/91‑1 (MQ=255)
cTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAg < 1:846990/91‑1 (MQ=255)
cTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAg < 2:1037514/91‑1 (MQ=255)
cTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAg < 1:98211/91‑1 (MQ=255)
aataCAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGccc > 1:91474/1‑91 (MQ=255)
cAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTg > 2:647931/1‑72 (MQ=255)
cAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTg < 1:647931/72‑1 (MQ=255)
aaGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGt > 1:632223/1‑91 (MQ=255)
aGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTc > 2:211334/1‑91 (MQ=255)
tAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAg > 1:637670/1‑91 (MQ=255)
agaATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAAc > 1:981539/1‑91 (MQ=255)
gaATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACg > 2:910435/1‑91 (MQ=255)
gaATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACg > 1:914677/1‑91 (MQ=255)
tGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGc < 2:815020/91‑1 (MQ=255)
aCTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTga > 1:338294/1‑91 (MQ=255)
tGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCa > 1:37278/1‑38 (MQ=37)
tGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCa < 2:37278/38‑1 (MQ=37)
tGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGAcc < 1:775387/46‑1 (MQ=255)
tGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGAcc > 2:775387/1‑46 (MQ=255)
cAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAg > 1:897963/1‑91 (MQ=255)
cAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAg > 1:547109/1‑91 (MQ=255)
atatGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGaa < 2:981539/91‑1 (MQ=255)
atatGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGaa < 1:915498/91‑1 (MQ=255)
atGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGc > 1:235476/1‑91 (MQ=255)
ccTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTAAAAGTCGAAGCTAt < 2:897963/91‑1 (MQ=255)
tGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTg > 2:813453/1‑91 (MQ=255)
tGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTg > 2:1026967/1‑91 (MQ=255)
tGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTg > 1:560782/1‑91 (MQ=255)
gTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGc > 1:238498/1‑91 (MQ=255)
|
ATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATACAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGC > NZ_CP009273/716039‑716211
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATACAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAA > NZ_CP009273/716027‑716220
|
GCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGC > SRR3722125.38974/1‑101 (MQ=60)
gcctagtctcgtgggctcggagatgtgtataagagacagGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATGCAGGCATATGCCTGTAATGCAG < SRR3722125.921694/62‑1 (MQ=60)
agtctcgtgggctcggagatgtgtataagagacagGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGA < SRR3722125.792252/66‑1 (MQ=60)
aagcagaagacggcatacgagatcatgcctagtctcgtgggctcggagatgtgtataagagacagCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGA < SRR3722125.537160/36‑1 (MQ=60)
CCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGA < SRR3722125.570874/101‑1 (MQ=60)
aagagacaGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAG < SRR3722125.661896/93‑1 (MQ=60)
GGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCC > SRR3722125.93182/1‑101 (MQ=60)
catacgatatcatgcctagtctcgtgggctcggagatgtgtataagagacaGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGT < SRR3722125.1141518/50‑1 (MQ=60)
CTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGT < SRR3722125.865629/101‑1 (MQ=60)
CTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGT < SRR3722125.100056/101‑1 (MQ=60)
CTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGT > SRR3722125.645839/1‑101 (MQ=60)
ATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAG > SRR3722125.651420/1‑101 (MQ=60)
gcctagtctcgtgggctcggagatgtgtataagagacagTGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAA < SRR3722125.737094/62‑1 (MQ=60)
GGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAAC > SRR3722125.1003464/1‑101 (MQ=60)
GGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACG > SRR3722125.934938/1‑101 (MQ=60)
GAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGA > SRR3722125.345334/1‑101 (MQ=60)
GAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGctgtctcttatacacatctccgagcccacgagactaggcatga > SRR3722125.37931/1‑58 (MQ=60)
CTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAG > SRR3722125.558824/1‑101 (MQ=60)
CTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAG > SRR3722125.917837/1‑101 (MQ=60)
ATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGC > SRR3722125.240187/1‑101 (MQ=60)
GGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTG > SRR3722125.572785/1‑101 (MQ=60)
GCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGC > SRR3722125.243268/1‑101 (MQ=60)
ATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGA < SRR3722125.935798/101‑1 (MQ=60)
TGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAA < SRR3722125.555700/101‑1 (MQ=60)
|
GCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATACAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAA > NZ_CP009273/716027‑716220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
