Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I102 R1
|
278 |
39.1 |
2251254 |
95.8% |
2156701 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
885,786 |
T→G |
L81R (CTG→CGG) |
ybjM → |
inner membrane protein YbjM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 885,786 | 0 | T | G | 100.0%
| 181.5
/ NA
| 49 | L81R (CTG→CGG) | ybjM | inner membrane protein YbjM |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (16/33); total (16/33) |
TTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTT > NZ_CP009273/885707‑885873
|
ttttCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCAc < 2:984130/91‑1 (MQ=255)
tCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGa < 2:1020547/91‑1 (MQ=255)
cacaGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGAc < 2:195021/91‑1 (MQ=255)
gCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACgcgc > 1:1122767/1‑91 (MQ=255)
gCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACgcgc > 2:595057/1‑91 (MQ=255)
cGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCt > 1:146404/1‑91 (MQ=255)
gTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc > 2:596183/1‑91 (MQ=255)
tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCa < 2:71056/91‑1 (MQ=255)
ccTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCaa > 1:683260/1‑91 (MQ=255)
tGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAaga < 2:658292/91‑1 (MQ=255)
aaaCCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGc < 1:730442/91‑1 (MQ=255)
ctctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc > 1:280668/1‑91 (MQ=255)
ctctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc > 2:335171/1‑91 (MQ=255)
ctctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc < 1:585406/91‑1 (MQ=255)
ctctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc > 1:541163/1‑91 (MQ=255)
ctctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc > 2:947425/1‑91 (MQ=255)
ctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCAt < 2:660085/91‑1 (MQ=255)
tttGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGt < 1:595057/91‑1 (MQ=255)
cAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAg < 2:146404/91‑1 (MQ=255)
aaTGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgc < 2:506157/91‑1 (MQ=255)
aTGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCa < 2:914759/68‑1 (MQ=255)
aTGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCa > 1:914759/1‑68 (MQ=255)
tGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcgc < 1:502331/91‑1 (MQ=255)
tGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcgc < 2:184945/91‑1 (MQ=255)
cTGGCTGCACCCTGTTCGATGCGCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACCAAGCGCgg < 2:116691/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGTCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 1:999628/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 1:307059/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 2:1122767/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 1:257523/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 2:1009969/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 1:818807/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 1:596183/91‑1 (MQ=255)
cTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg < 1:579833/91‑1 (MQ=255)
ggcACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTc < 1:668823/91‑1 (MQ=255)
ggcACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTc < 2:826579/91‑1 (MQ=255)
cACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTg > 2:275122/1‑91 (MQ=255)
tGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGtgtg < 2:683260/91‑1 (MQ=255)
gTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATgg < 1:846686/65‑1 (MQ=255)
gTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATgg > 2:846686/1‑65 (MQ=255)
gATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTg < 1:335171/91‑1 (MQ=255)
tGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTggg < 1:61981/91‑1 (MQ=255)
cTCATTGTGCGG‑GGGTTTTTTCACCGTCGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGc < 1:444960/91‑1 (MQ=255)
tCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGc < 2:1068688/91‑1 (MQ=255)
tCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGc > 2:965290/1‑91 (MQ=255)
tCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGc < 1:474808/91‑1 (MQ=255)
cATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc < 1:445998/51‑1 (MQ=255)
cATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGc > 2:445998/1‑51 (MQ=255)
cATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCa > 1:685035/1‑91 (MQ=255)
ttATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACt < 1:1025445/91‑1 (MQ=255)
aTGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtgt < 1:231047/91‑1 (MQ=255)
ggCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGttt > 2:1003533/1‑91 (MQ=255)
|
TTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTT > NZ_CP009273/885707‑885873
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATT > NZ_CP009273/885705‑885879
|
TCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGC > SRR3722125.1148119/1‑101 (MQ=60)
CTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCT > SRR3722125.149195/1‑101 (MQ=60)
GTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAA > SRR3722125.697997/1‑101 (MQ=60)
GTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGC > SRR3722125.286360/1‑101 (MQ=60)
GTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGC > SRR3722125.552716/1‑101 (MQ=60)
AAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTT < SRR3722125.746171/101‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTA < SRR3722125.455411/71‑1 (MQ=60)
CTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTA < SRR3722125.597982/101‑1 (MQ=60)
tgtgtataagagacaGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCG < SRR3722125.865314/86‑1 (MQ=60)
GTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCActgtctcttatac > SRR3722125.935021/1‑88 (MQ=60)
TTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGC < SRR3722125.607857/101‑1 (MQ=60)
TGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGG < SRR3722125.512962/101‑1 (MQ=60)
CTGGCGGCCCCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTG < SRR3722125.313360/101‑1 (MQ=60)
CTGGCGGCACCCTGTTCGATGCTCATTATGCGTCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTATTCTGGTG < SRR3722125.1021966/101‑1 (MQ=60)
CTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTG < SRR3722125.592253/101‑1 (MQ=60)
CTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTG < SRR3722125.836750/101‑1 (MQ=60)
CTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTG < SRR3722125.609004/101‑1 (MQ=60)
CTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTG < SRR3722125.262710/101‑1 (MQ=60)
GGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGC < SRR3722125.683219/101‑1 (MQ=60)
GTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCA > SRR3722125.699817/1‑101 (MQ=60)
GATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGT < SRR3722125.342114/101‑1 (MQ=60)
TGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGT < SRR3722125.63131/101‑1 (MQ=60)
TCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTC > SRR3722125.306841/1‑101 (MQ=60)
TCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTC < SRR3722125.484820/101‑1 (MQ=60)
CATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTAGTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCT > SRR3722125.876925/1‑101 (MQ=60)
TTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTA < SRR3722125.1048420/101‑1 (MQ=60)
ATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATT < SRR3722125.235664/101‑1 (MQ=60)
|
TCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATT > NZ_CP009273/885705‑885879
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
