Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I96 R1
|
227 |
23.3 |
882352 |
98.0% |
864704 |
126.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
890,897 |
T→C |
S151P (TCC→CCC) |
potG → |
putrescine ABC transporter ATP‑binding subunit PotG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 890,897 | 0 | T | C | 100.0%
| 69.0
/ NA
| 20 | S151P (TCC→CCC) | potG | putrescine ABC transporter ATP‑binding subunit PotG |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/11); total (9/11) |
TGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGG > NZ_CP009273/890763‑891026
|
tGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGAGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCg < 1:4585/138‑1 (MQ=255)
cGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCggtggt < 2:347708/139‑1 (MQ=255)
tGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCggtggt > 1:328924/1‑137 (MQ=255)
aTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACgtg < 2:238578/139‑1 (MQ=255)
aTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACgtg < 2:166104/139‑1 (MQ=255)
cGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGtgtg > 1:251878/1‑139 (MQ=255)
ttGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGtggccc < 2:306253/138‑1 (MQ=255)
gACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTg > 1:91960/1‑139 (MQ=255)
aCTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAg > 2:427527/1‑139 (MQ=255)
ccGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGcc < 2:251878/139‑1 (MQ=255)
ccGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGAt < 1:380951/139‑1 (MQ=255)
tGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGAt > 1:441174/1‑133 (MQ=255)
cTNGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGgcgcg < 2:441174/138‑1 (MQ=255)
cTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGgcgcgc < 1:266307/139‑1 (MQ=255)
tCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCt > 2:269233/1‑139 (MQ=255)
atGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGc > 1:349270/1‑139 (MQ=255)
ggAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGa > 1:217779/1‑138 (MQ=255)
gTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAg > 2:303785/1‑138 (MQ=255)
cGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGAt < 1:269233/138‑1 (MQ=255)
cATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGtggtgg < 2:217779/139‑1 (MQ=255)
|
TGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGG > NZ_CP009273/890763‑891026
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAG > NZ_CP009273/890758‑891020
|
GCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTC > SRR3722119.332269/1‑149 (MQ=60)
ATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGAGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGA < SRR3722119.4628/150‑1 (MQ=60)
GAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGG > SRR3722119.254508/1‑151 (MQ=60)
GCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGC > SRR3722119.92856/1‑151 (MQ=60)
ACCGACAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTCCGAAGCGCCaggaacaatt > SRR3722119.175309/1‑140 (MQ=60)
GCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGG < SRR3722119.384782/151‑1 (MQ=60)
GGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATG > SRR3722119.445577/1‑145 (MQ=60)
GCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAG < SRR3722119.269051/151‑1 (MQ=60)
GCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCG > SRR3722119.352806/1‑151 (MQ=60)
GTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGAC > SRR3722119.220115/1‑150 (MQ=60)
TCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAG < SRR3722119.272019/150‑1 (MQ=60)
|
GCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAG > NZ_CP009273/890758‑891020
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
