Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I88 R1
|
217 |
30.5 |
1742422 |
95.9% |
1670982 |
87.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
900,176 |
T→G |
V43G (GTT→GGT) |
ybjQ → |
heavy metal‑binding domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 900,176 | 0 | T | G | 100.0%
| 70.4
/ NA
| 20 | V43G (GTT→GGT) | ybjQ | heavy metal‑binding domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (9/11); total (9/11) |
TGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGG > NZ_CP009273/900093‑900253
|
tGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGa < 2:225730/91‑1 (MQ=255)
gAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAc > 1:223797/1‑91 (MQ=255)
gAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAc > 1:236529/1‑91 (MQ=255)
gtgtGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGc > 1:695148/1‑91 (MQ=255)
gtggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGt > 1:673915/1‑91 (MQ=255)
ggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAt < 1:714143/91‑1 (MQ=255)
gAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAAc < 1:729651/91‑1 (MQ=255)
gAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAAc < 2:199040/91‑1 (MQ=255)
gATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGt < 2:558574/91‑1 (MQ=255)
gATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGt < 2:685010/91‑1 (MQ=255)
ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa < 1:548552/91‑1 (MQ=255)
gTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACg < 2:515150/91‑1 (MQ=255)
ccAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGga > 1:442153/1‑91 (MQ=255)
ccAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGga > 2:626143/1‑91 (MQ=255)
tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATc < 1:333934/91‑1 (MQ=255)
tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATc < 1:330876/91‑1 (MQ=255)
cGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGCTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTg > 1:844704/1‑91 (MQ=255)
aTCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAgg > 2:295357/1‑91 (MQ=255)
tatCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa > 1:177589/1‑45 (MQ=255)
tatCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa < 2:177589/45‑1 (MQ=255)
|
TGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGG > NZ_CP009273/900093‑900253
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCT > NZ_CP009273/900084‑900263
|
GACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAC < SRR3722118.197370/101‑1 (MQ=60)
GACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAC > SRR3722118.228324/1‑101 (MQ=60)
GACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAC > SRR3722118.241301/1‑101 (MQ=60)
GAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGC > SRR3722118.710300/1‑101 (MQ=60)
ATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGT > SRR3722118.688524/1‑101 (MQ=60)
GGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGTAC < SRR3722118.729813/101‑1 (MQ=60)
GAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGC < SRR3722118.745682/101‑1 (MQ=60)
ATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGA > SRR3722118.451477/1‑101 (MQ=60)
TTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAG < SRR3722118.560186/101‑1 (MQ=60)
CAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGCTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTG > SRR3722118.863375/1‑101 (MQ=60)
TATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGG < SRR3722118.337683/101‑1 (MQ=60)
TATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGG < SRR3722118.340808/101‑1 (MQ=60)
GTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGctgtctcttatacacatctccgagcccacgagacgg > SRR3722118.181079/1‑65 (MQ=60)
ATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCT > SRR3722118.258549/1‑101 (MQ=60)
|
GACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCT > NZ_CP009273/900084‑900263
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
