Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I102 R1
|
278 |
39.1 |
2251254 |
95.8% |
2156701 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
900,176 |
T→G |
V43G (GTT→GGT) |
ybjQ → |
heavy metal‑binding domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 900,176 | 0 | T | G | 100.0%
| 81.3
/ NA
| 23 | V43G (GTT→GGT) | ybjQ | heavy metal‑binding domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (7/16); total (7/16) |
TCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCG > NZ_CP009273/900089‑900261
|
tCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTgg > 1:251023/1‑91 (MQ=255)
gTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCg < 2:1116078/91‑1 (MQ=255)
tGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGa < 2:798134/91‑1 (MQ=255)
tGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGa < 2:251023/91‑1 (MQ=255)
gAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAc > 2:137168/1‑91 (MQ=255)
gCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCgg > 1:1040439/1‑91 (MQ=255)
gCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCgg < 1:427671/91‑1 (MQ=255)
ggTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTg < 1:968278/91‑1 (MQ=255)
ggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAt < 2:901127/91‑1 (MQ=255)
ggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAt < 2:501390/91‑1 (MQ=255)
ggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAt < 2:546503/91‑1 (MQ=255)
ggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAt < 2:654005/91‑1 (MQ=255)
gtgAGCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATg > 1:905567/1‑91 (MQ=255)
gCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGa > 1:893078/1‑91 (MQ=255)
gAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAAc < 1:15423/91‑1 (MQ=255)
ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa < 1:7437/91‑1 (MQ=255)
ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa < 2:188742/91‑1 (MQ=255)
gTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTCTGAAAAAGAACTGCGTAAAGCACg < 2:1078342/91‑1 (MQ=255)
gTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACg < 1:222064/91‑1 (MQ=255)
tttCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGcc > 2:158397/1‑91 (MQ=255)
gTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGa < 2:921160/91‑1 (MQ=255)
cgcgATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGgcgc < 2:1040221/91‑1 (MQ=255)
tatCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGgcg > 1:1092300/1‑91 (MQ=255)
|
TCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCG > NZ_CP009273/900089‑900261
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCC > NZ_CP009273/900079‑900276
|
GGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGG > SRR3722125.256079/1‑101 (MQ=60)
CAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGG < SRR3722125.1050917/101‑1 (MQ=60)
GACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAC < SRR3722125.343067/101‑1 (MQ=60)
GTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGG > SRR3722125.1063767/1‑101 (MQ=60)
TTGCGGTGTGGTGAGCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATG > SRR3722125.925636/1‑101 (MQ=60)
GCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAA < SRR3722125.436686/101‑1 (MQ=60)
GGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAA < SRR3722125.989875/101‑1 (MQ=60)
GTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGA > SRR3722125.912844/1‑101 (MQ=60)
GAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGC < SRR3722125.15708/101‑1 (MQ=60)
TTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAG < SRR3722125.7578/101‑1 (MQ=60)
GTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCC < SRR3722125.226458/101‑1 (MQ=60)
GTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCG > SRR3722125.1116878/1‑101 (MQ=60)
GTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCtaattc > SRR3722125.291513/1‑95 (MQ=60)
|
GGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCC > NZ_CP009273/900079‑900276
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
