Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I88 R1
|
217 |
30.5 |
1742422 |
95.9% |
1670982 |
87.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
485,143 |
A→G |
D1051G (GAT→GGT) |
mscK → |
mechanosensitive channel MscK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 485,143 | 0 | A | G | 100.0%
| 154.2
/ NA
| 41 | D1051G (GAT→GGT) | mscK | mechanosensitive channel MscK |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/28); total (13/28) |
AAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCC > NZ_CP009273/485061‑485229
|
aaGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGc < 1:751083/91‑1 (MQ=255)
aaGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGc < 2:488590/91‑1 (MQ=255)
aGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt > 2:540908/1‑91 (MQ=255)
aGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt > 1:535566/1‑91 (MQ=255)
aGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt > 2:779975/1‑91 (MQ=255)
ggcggcGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTg < 2:209712/91‑1 (MQ=255)
cggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCg < 1:328628/91‑1 (MQ=255)
cggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCg < 1:391259/91‑1 (MQ=255)
tGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTAt < 1:94416/91‑1 (MQ=255)
tGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTAt < 1:674879/91‑1 (MQ=255)
cAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGa < 2:383060/91‑1 (MQ=255)
cAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGa < 1:779975/91‑1 (MQ=255)
gATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGt < 2:349198/91‑1 (MQ=255)
gATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGt > 2:420251/1‑91 (MQ=255)
gATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGt < 2:127131/91‑1 (MQ=255)
aTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCAt < 2:789356/60‑1 (MQ=255)
aTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCAt > 1:789356/1‑60 (MQ=255)
aTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTg > 1:188946/1‑91 (MQ=255)
aTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTg > 2:870125/1‑91 (MQ=255)
aCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg > 1:699046/1‑91 (MQ=255)
gAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTa < 2:645010/91‑1 (MQ=255)
aTGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTa > 1:372319/1‑91 (MQ=255)
tgtcGGAAGTATTCTCTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTTCGTCTGTATGTGGGTGAACTGCGTGACCGTAGTCGTAc < 2:403265/88‑1 (MQ=255)
gCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACt < 2:659608/91‑1 (MQ=255)
aaGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGa < 1:485939/91‑1 (MQ=255)
ttcttTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGc < 2:593949/91‑1 (MQ=255)
ttcttTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGc < 1:540908/91‑1 (MQ=255)
tcttTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCt > 1:149568/1‑91 (MQ=255)
cttTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg < 1:383558/91‑1 (MQ=255)
tACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAAc < 1:870125/91‑1 (MQ=255)
aCGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAAc > 2:244680/1‑90 (MQ=255)
aCGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAAc < 1:244680/90‑1 (MQ=255)
ggCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGt < 2:382640/91‑1 (MQ=255)
aTTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt < 2:302471/91‑1 (MQ=255)
aTTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt > 1:518441/1‑91 (MQ=255)
aTTTGGCGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt < 1:846871/91‑1 (MQ=255)
gTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGa < 2:699046/91‑1 (MQ=255)
gTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGa < 2:822444/91‑1 (MQ=255)
gCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATc < 1:632589/91‑1 (MQ=255)
gTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGc < 1:495981/91‑1 (MQ=255)
ttGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGGACTATCGAACAGGtgtggc > 1:153524/1‑89 (MQ=255)
|
AAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCC > NZ_CP009273/485061‑485229
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACG > NZ_CP009273/485044‑485238
|
TGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGT < SRR3722118.331189/101‑1 (MQ=60)
CGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCA < SRR3722118.847578/101‑1 (MQ=60)
AAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGA < SRR3722118.716302/101‑1 (MQ=60)
AGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGC < SRR3722118.279474/101‑1 (MQ=60)
AGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGC < SRR3722118.374505/101‑1 (MQ=60)
GTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCT > SRR3722118.546929/1‑101 (MQ=60)
AAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTA < SRR3722118.767559/101‑1 (MQ=60)
CGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTG < SRR3722118.399471/101‑1 (MQ=60)
CGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTG < SRR3722118.335389/101‑1 (MQ=60)
TGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAAC < SRR3722118.689513/101‑1 (MQ=60)
TGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAAC < SRR3722118.96143/101‑1 (MQ=60)
CCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCctgtctcttatacacatctcc > SRR3722118.806695/1‑80 (MQ=60)
CCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTG > SRR3722118.192688/1‑101 (MQ=60)
CAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGAC < SRR3722118.797091/101‑1 (MQ=60)
AGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCG > SRR3722118.714298/1‑101 (MQ=60)
GCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTA > SRR3722118.380102/1‑101 (MQ=60)
CCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT > SRR3722118.152441/1‑101 (MQ=60)
AAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAAC < SRR3722118.496217/101‑1 (MQ=60)
TTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGCAC < SRR3722118.552381/101‑1 (MQ=60)
TCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACT > SRR3722118.529440/1‑101 (MQ=60)
CTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTA < SRR3722118.391597/101‑1 (MQ=60)
TACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCG < SRR3722118.249638/101‑1 (MQ=60)
TACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCG < SRR3722118.889513/101‑1 (MQ=60)
ATTTGGCGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGC < SRR3722118.865595/101‑1 (MQ=60)
TGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGGACTATCGAACAGGTGTGGC > SRR3722118.156467/1‑101 (MQ=60)
GCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCG < SRR3722118.646228/101‑1 (MQ=60)
GTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACG < SRR3722118.506492/101‑1 (MQ=60)
|
TGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACG > NZ_CP009273/485044‑485238
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
