Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I184 R1
|
278 |
29.6 |
1166432 |
97.3% |
1134938 |
124.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
485,143 |
A→G |
D1051G (GAT→GGT) |
mscK → |
mechanosensitive channel MscK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 485,143 | 0 | A | G | 100.0%
| 135.0
/ NA
| 38 | D1051G (GAT→GGT) | mscK | mechanosensitive channel MscK |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/18); total (20/18) |
GTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGC > NZ_CP009273/485008‑485262
|
gTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCa > 1:311290/1‑139 (MQ=255)
tctcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCAt > 2:378071/1‑139 (MQ=255)
tctcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCAt > 1:37848/1‑139 (MQ=255)
ggCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAg < 2:486330/138‑1 (MQ=255)
gCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt < 2:102051/139‑1 (MQ=255)
gCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt < 1:418102/139‑1 (MQ=255)
gCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt < 2:331848/139‑1 (MQ=255)
gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGt < 2:153592/138‑1 (MQ=255)
gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTc > 1:572958/1‑139 (MQ=255)
tGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTa < 2:572958/138‑1 (MQ=255)
ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCg > 2:350952/1‑139 (MQ=255)
cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGt > 1:421829/1‑139 (MQ=255)
cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGt > 2:208986/1‑139 (MQ=255)
cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGt > 1:517209/1‑139 (MQ=255)
cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGt > 1:536130/1‑139 (MQ=255)
aTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCCTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTg > 2:529579/1‑138 (MQ=255)
aGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt < 2:104353/111‑1 (MQ=255)
aGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt > 1:104353/1‑111 (MQ=255)
aGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGAc < 2:517209/139‑1 (MQ=255)
gCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg > 1:404430/1‑138 (MQ=255)
tgtTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc < 2:421829/139‑1 (MQ=255)
ggcggcGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg < 2:50331/139‑1 (MQ=255)
ggcggcGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCCTGACCGTAGTCGTACTGTCGATGAg > 1:78258/1‑139 (MQ=255)
ggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg < 1:377574/139‑1 (MQ=255)
ggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg < 2:37848/139‑1 (MQ=255)
ggcgACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCCTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg < 1:529579/139‑1 (MQ=255)
cTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCg > 1:458912/1‑139 (MQ=255)
gCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTGACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt < 2:375730/139‑1 (MQ=255)
ccAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaa < 1:54358/139‑1 (MQ=255)
aaTGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt < 1:424529/54‑1 (MQ=255)
aaTGCCGGAAGTCTTCTTTACGGCATGTGGTGCCAGCACGTTGGGTCATGAGCt > 2:424529/1‑54 (MQ=255)
tGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTa < 2:316275/61‑1 (MQ=255)
tGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTa > 1:316275/1‑61 (MQ=255)
aGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt > 1:103172/1‑45 (MQ=255)
aGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCt < 2:103172/45‑1 (MQ=255)
ttcttTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTg > 2:175314/1‑139 (MQ=255)
tACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCtt > 2:535947/1‑138 (MQ=255)
tttGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGc > 1:112430/1‑139 (MQ=255)
|
GTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGC > NZ_CP009273/485008‑485262
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCT > NZ_CP009273/484997‑485263
|
TCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCAT > SRR3722139.315147/1‑151 (MQ=60)
CTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATG > SRR3722139.38262/1‑151 (MQ=60)
GTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCT > SRR3722139.579508/1‑151 (MQ=60)
GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATG < SRR3722139.423051/151‑1 (MQ=60)
ttttttttcaagcagaagacggcatacgagatctagtacggtctcgtgggctcggagatgtgtataagagacaGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATG < SRR3722139.429531/77‑1 (MQ=60)
GCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTG > SRR3722139.542286/1‑151 (MQ=60)
GCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTG > SRR3722139.523151/1‑151 (MQ=60)
GCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTG > SRR3722139.426802/1‑151 (MQ=60)
GATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGctgtctcttatacacatc > SRR3722139.105595/1‑133 (MQ=60)
CTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGT > SRR3722139.409220/1‑150 (MQ=60)
GTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCCTGACCGTAGTCGTACTGTCGATGAGC > SRR3722139.79161/1‑151 (MQ=60)
GAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGT > SRR3722139.464290/1‑151 (MQ=60)
CGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTAT < SRR3722139.382086/151‑1 (MQ=60)
CGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCCTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTAT < SRR3722139.535675/151‑1 (MQ=60)
GCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtcttctgcttga > SRR3722139.320182/1‑83 (MQ=60)
ACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAAC < SRR3722139.54987/151‑1 (MQ=60)
CCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGctgtctcttatacacatctccgagcccacgagaccgtactagatatcgtatgccgtcttctgcttgaaaaaaaaaagaactgat > SRR3722139.104397/1‑67 (MQ=60)
TCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCT > SRR3722139.113787/1‑151 (MQ=60)
|
TCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCT > NZ_CP009273/484997‑485263
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
