Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I88 R1
|
217 |
30.5 |
1742422 |
95.9% |
1670982 |
87.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
987,566 |
A→T |
I497F (ATC→TTC) |
pepN → |
aminopeptidase N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 987,566 | 0 | A | T | 100.0%
| 81.4
/ NA
| 24 | I497F (ATC→TTC) | pepN | aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/13); total (11/13) |
ACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTA > NZ_CP009273/987482‑987651
|
aCGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGt > 1:668936/1‑91 (MQ=255)
gCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGc > 2:511/1‑91 (MQ=255)
gATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTc > 2:444855/1‑91 (MQ=255)
cAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATc < 2:83893/91‑1 (MQ=255)
cAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATc < 1:563633/91‑1 (MQ=255)
gAAAAACAGCCGCTGCATGTTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGa > 1:545890/1‑91 (MQ=255)
cAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCg < 1:399262/91‑1 (MQ=255)
gCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAAc < 2:37639/91‑1 (MQ=255)
ttGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGTAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGc < 1:545232/91‑1 (MQ=255)
ttGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGc < 2:133934/91‑1 (MQ=255)
ttGCCAGCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGc < 2:143409/91‑1 (MQ=255)
cATCGAACTGTATGATAACGAAGGCAAAGCGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGACCGTCACTCAGGCGGaa > 2:651516/1‑91 (MQ=255)
tGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCtt < 1:274214/91‑1 (MQ=255)
tGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCtt < 1:821709/91‑1 (MQ=255)
tGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTc > 1:450422/1‑91 (MQ=255)
tAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTACGTGCTGACAGTCACTCAGGCGGAACAGACCTTTGTCttt > 2:650534/1‑91 (MQ=255)
gAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATa > 2:172326/1‑91 (MQ=255)
gAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATa > 2:127293/1‑91 (MQ=255)
aaGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATaa < 2:483823/91‑1 (MQ=255)
aGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTc > 2:32065/1‑58 (MQ=255)
aGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTc < 1:32065/58‑1 (MQ=255)
cAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTc < 2:852410/91‑1 (MQ=255)
aaaGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCt < 2:31600/91‑1 (MQ=255)
aaGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTa > 1:270226/1‑91 (MQ=255)
|
ACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTA > NZ_CP009273/987482‑987651
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCA > NZ_CP009273/987472‑987657
|
CAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGT > SRR3722118.683430/1‑101 (MQ=60)
GGATCAGGCAGAAAAACAGCCGCTGCATGTTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGA > SRR3722118.557470/1‑101 (MQ=60)
CAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTC < SRR3722118.575572/101‑1 (MQ=60)
CAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGT < SRR3722118.407637/101‑1 (MQ=60)
tcggagatgtgtataagagacagATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGG < SRR3722118.32647/78‑1 (MQ=60)
TTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGTAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACC < SRR3722118.556803/101‑1 (MQ=60)
TCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTC > SRR3722118.459915/1‑101 (MQ=60)
TGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGAT < SRR3722118.279831/101‑1 (MQ=60)
TGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGAT < SRR3722118.839794/101‑1 (MQ=60)
AACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTA > SRR3722118.275752/1‑101 (MQ=60)
GGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCA > SRR3722118.90843/1‑101 (MQ=60)
|
CAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCA > NZ_CP009273/987472‑987657
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
